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The map label for this gene is nhaA

Identifier: 126209405

GI number: 126209405

Start: 2177937

End: 2179121

Strand: Direct

Name: nhaA

Synonym: APL_1947

Alternate gene names: 126209405

Gene position: 2177937-2179121 (Clockwise)

Preceding gene: 126209398

Following gene: 126209406

Centisome position: 95.76

GC content: 42.36

Gene sequence:

>1185_bases
ATGTTAAAACAAATTCAGAAATTTCTGAAATTAGAAGCGGCGAGTGGTATCTTGCTATTGGTATCGGCACTACTTGCGAT
GATTTTTGCCAATACTGATTTGAATCAGTTATATTTTAGTTTTCTACAAACGGAAGTAGCGATTAAATTCGGTGCTTTTA
GTATTGATAAGCCGTTATTAATGTGGGTAAACGACGGCTTTATGGCGGTCTTTTTTATCTTAGTCGGTATGGAAGTAAAA
AGAGAGCTGTTTGAGGGCTCGCTTTCAAGCTATCAAAAAGCGATCTTCCCTGCCGTTGCCGCGCTGGGGGGGATGGTTGT
CCCTGCTTTGGTTTATTGGTTTATTAACCAAAATCATCCGGAATATCAATCAGGTTGGGCGATTCCGATGGCAACCGACA
TTGCCTTTGCATTAGGAATTGTCGCCTTACTCAGTAAACAGGTGCCGCCGGCACTCAAAGTATTTTTACTTGCATTGGCG
ATTATTGACGACTTAGGGGCGATTATCGTGATTGCCTTATTCTTCTCGCATGAGATGAGTATGCAAGCTTTAACTATTGC
AAGTATCGCGATTGTTATTTTAGTGGCAATGAATCGTTATAAAGTAACCGGATTAATCAACTACGCCATTATCGGTACTA
TTCTTTGGGCTTCGGTTTTAAAATCCGGAGTACACGCAACCTTAGCGGGTGTGATTATCGGTTTCTGTATTCCGTTGCGC
GGTAAAAACGGCGAAGCGCCGTTACATCATTTGGAACATGCGCTTGCCCCGTGGTGTTCGTTTGCGATTTTACCGTTATT
CGCTTTCTCGAATGCCGGCGTATCGCTGGAAGGTATGAGCTTGGATAAATTAGCCTCTCCGTTACCATTAGGGGTGGCAT
TAGGTTTAATTATCGGTAAACCGGTCGGTGTTTTCTTATTTAGCTATGTAGCCGTATTGTTAGGTATAGCAAAAGTACCT
GAAGGTATCAATTTAAAACAAATTTTCGCGATTGCGGTACTTTGCGGTATCGGGTTTACTATGTCGATGTTTATTGCCGG
ACTTGCATTCGGAGAGGAAGAGGCGAGTGAAAATATGCTTGCCTTAGCTCGTTTAGGTATTCTCATGGGTACATTTGTTT
CGGCGATAATCGGTTATTTCTTATTGAAAATCACGACGAAGCCAAGCCTAATGAAAGCGGCGTAA

Upstream 100 bases:

>100_bases
TTGCTTTTGTATTCGGTTAAAAAAATGTTAAGGTCGATATTAAGTTTATTGGCTATACGATCGAAGGTTTTCGGTCGTAC
TATAGAGGAGATTTTTTTGC

Downstream 100 bases:

>100_bases
GCGGTCTGATTTCAGTCGTTTTTCACAAACTAAATGCCTCAGCGAATCGGTTGGGGCATTTTCATTTCAACCGTTCGGGA
ACTTTCAGGTGTAACATCAT

Product: pH-dependent sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLKQIQKFLKLEAASGILLLVSALLAMIFANTDLNQLYFSFLQTEVAIKFGAFSIDKPLLMWVNDGFMAVFFILVGMEVK
RELFEGSLSSYQKAIFPAVAALGGMVVPALVYWFINQNHPEYQSGWAIPMATDIAFALGIVALLSKQVPPALKVFLLALA
IIDDLGAIIVIALFFSHEMSMQALTIASIAIVILVAMNRYKVTGLINYAIIGTILWASVLKSGVHATLAGVIIGFCIPLR
GKNGEAPLHHLEHALAPWCSFAILPLFAFSNAGVSLEGMSLDKLASPLPLGVALGLIIGKPVGVFLFSYVAVLLGIAKVP
EGINLKQIFAIAVLCGIGFTMSMFIAGLAFGEEEASENMLALARLGILMGTFVSAIIGYFLLKITTKPSLMKAA

Sequences:

>Translated_394_residues
MLKQIQKFLKLEAASGILLLVSALLAMIFANTDLNQLYFSFLQTEVAIKFGAFSIDKPLLMWVNDGFMAVFFILVGMEVK
RELFEGSLSSYQKAIFPAVAALGGMVVPALVYWFINQNHPEYQSGWAIPMATDIAFALGIVALLSKQVPPALKVFLLALA
IIDDLGAIIVIALFFSHEMSMQALTIASIAIVILVAMNRYKVTGLINYAIIGTILWASVLKSGVHATLAGVIIGFCIPLR
GKNGEAPLHHLEHALAPWCSFAILPLFAFSNAGVSLEGMSLDKLASPLPLGVALGLIIGKPVGVFLFSYVAVLLGIAKVP
EGINLKQIFAIAVLCGIGFTMSMFIAGLAFGEEEASENMLALARLGILMGTFVSAIIGYFLLKITTKPSLMKAA
>Mature_394_residues
MLKQIQKFLKLEAASGILLLVSALLAMIFANTDLNQLYFSFLQTEVAIKFGAFSIDKPLLMWVNDGFMAVFFILVGMEVK
RELFEGSLSSYQKAIFPAVAALGGMVVPALVYWFINQNHPEYQSGWAIPMATDIAFALGIVALLSKQVPPALKVFLLALA
IIDDLGAIIVIALFFSHEMSMQALTIASIAIVILVAMNRYKVTGLINYAIIGTILWASVLKSGVHATLAGVIIGFCIPLR
GKNGEAPLHHLEHALAPWCSFAILPLFAFSNAGVSLEGMSLDKLASPLPLGVALGLIIGKPVGVFLFSYVAVLLGIAKVP
EGINLKQIFAIAVLCGIGFTMSMFIAGLAFGEEEASENMLALARLGILMGTFVSAIIGYFLLKITTKPSLMKAA

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=390, Percent_Identity=53.0769230769231, Blast_Score=387, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA_ACTP2 (A3N3N9)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054630.1
- ProteinModelPortal:   A3N3N9
- SMR:   A3N3N9
- STRING:   A3N3N9
- GeneID:   4848576
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1947
- eggNOG:   COG3004
- HOGENOM:   HBG665619
- OMA:   QKAIFPA
- PhylomeDB:   A3N3N9
- ProtClustDB:   PRK09560
- BioCyc:   APLE416269:APL_1947-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 42230; Mature: 42230

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1ee26b18)-; HASH(0x1f1b8650)-; HASH(0x1ef36ed8)-; HASH(0x1f1d6c0c)-; HASH(0x1f55186c)-; HASH(0x1b29bd10)-; HASH(0x1dfe10f4)-; HASH(0x1f4356d0)-; HASH(0x1f54f690)-; HASH(0x1f0181a8)-; HASH(0x1b5968b0)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQIQKFLKLEAASGILLLVSALLAMIFANTDLNQLYFSFLQTEVAIKFGAFSIDKPLL
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEECCCCEE
MWVNDGFMAVFFILVGMEVKRELFEGSLSSYQKAIFPAVAALGGMVVPALVYWFINQNHP
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EYQSGWAIPMATDIAFALGIVALLSKQVPPALKVFLLALAIIDDLGAIIVIALFFSHEMS
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQALTIASIAIVILVAMNRYKVTGLINYAIIGTILWASVLKSGVHATLAGVIIGFCIPLR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKNGEAPLHHLEHALAPWCSFAILPLFAFSNAGVSLEGMSLDKLASPLPLGVALGLIIGK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHCCCCCHHHHHHHHHCC
PVGVFLFSYVAVLLGIAKVPEGINLKQIFAIAVLCGIGFTMSMFIAGLAFGEEEASENML
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ALARLGILMGTFVSAIIGYFLLKITTKPSLMKAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCH
>Mature Secondary Structure
MLKQIQKFLKLEAASGILLLVSALLAMIFANTDLNQLYFSFLQTEVAIKFGAFSIDKPLL
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEECCCCEE
MWVNDGFMAVFFILVGMEVKRELFEGSLSSYQKAIFPAVAALGGMVVPALVYWFINQNHP
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EYQSGWAIPMATDIAFALGIVALLSKQVPPALKVFLLALAIIDDLGAIIVIALFFSHEMS
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQALTIASIAIVILVAMNRYKVTGLINYAIIGTILWASVLKSGVHATLAGVIIGFCIPLR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKNGEAPLHHLEHALAPWCSFAILPLFAFSNAGVSLEGMSLDKLASPLPLGVALGLIIGK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHCCCCCHHHHHHHHHCC
PVGVFLFSYVAVLLGIAKVPEGINLKQIFAIAVLCGIGFTMSMFIAGLAFGEEEASENML
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ALARLGILMGTFVSAIIGYFLLKITTKPSLMKAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA