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The map label for this gene is birA [H]

Identifier: 126209312

GI number: 126209312

Start: 2081220

End: 2081984

Strand: Reverse

Name: birA [H]

Synonym: APL_1850

Alternate gene names: 126209312

Gene position: 2081984-2081220 (Counterclockwise)

Preceding gene: 126209313

Following gene: 126209311

Centisome position: 91.54

GC content: 42.48

Gene sequence:

>765_bases
ATGAGCAAATTAAACCAATCTCAGATCCAAACGGCATTATTGTGCGGGCAAGCGTTGGTGTTTGATGAAATTGATTCGAC
CAACGAATATTTGCTTAAACACTATCAAACCTTGCCACAAGGTTCGGTATGTTTGGCGGAGCAACAAACCGCCGGCAGAG
GCAGACGTGGGCGCACTTGGTATTCACCGCAAAGTGAAAATGTCTATTTTTCGATTTTATGGCATTACCCGACTGAAGAC
GCTGCGCATATTTCTTCGCTGAGTTTGGTGGTTTCACTGATTATTGCTGAAAGTTTGCAAGCGCAACAGGTTGAAAATAT
CCAAATCAAATGGCCGAATGATATTTATTATTGTGGCAAAAAAATGGGCGGGATTCTGATTGAAACTAAAGCGGATCGCA
GTGGTATCCATTTAGTGATCGGCATCGGTTTAAATTTGGGAATGACCAAAGTGGATGAAAAGATCGTGACTCAAGCGTGG
TCTGATTTATCCGACTATCAATTTGATCGTAACGCATTAGTTTGTCGCCTTGCGTATGAATTGCAAAAAAATCTTAAAAT
TTACCCGCTTGTCGGCTTTGAACATTATGCCGAGCGTTGGCAAACGTTCGATATCTTTCGTGATCAGCCGGTAAAACTGG
TGACGGAAGGTAACGAAATTCACGGTATTTCACGAGGGATCAATCAACAAGGCGAATTATTGCTCGAACAAAACGGTACA
ATTACGCCGTTCGGTATCGGCGAAATGTCTTTAAGAGCGGAATAA

Upstream 100 bases:

>100_bases
GTGTACGTGAAAAATTCGGGGTTGAGCTGCATCCGGAAGTACGTTTTATGGGCAAAAATGGCGAAGTGAATAGCGAAGAA
GTCACGAGATAACCAAAGTT

Downstream 100 bases:

>100_bases
TACGCTTGCTTTCATAAGCTCTCTTTACTTTTATAAAGCAAAGAGAGCTTTTTCATTTCTGTGATCTGCGCCACACTTTC
ACAAAAAATTTACATCTAAT

Product: bifunctional biotin operon repressor/biotin synthetase BirA

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSKLNQSQIQTALLCGQALVFDEIDSTNEYLLKHYQTLPQGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHYPTED
AAHISSLSLVVSLIIAESLQAQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGIHLVIGIGLNLGMTKVDEKIVTQAW
SDLSDYQFDRNALVCRLAYELQKNLKIYPLVGFEHYAERWQTFDIFRDQPVKLVTEGNEIHGISRGINQQGELLLEQNGT
ITPFGIGEMSLRAE

Sequences:

>Translated_254_residues
MSKLNQSQIQTALLCGQALVFDEIDSTNEYLLKHYQTLPQGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHYPTED
AAHISSLSLVVSLIIAESLQAQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGIHLVIGIGLNLGMTKVDEKIVTQAW
SDLSDYQFDRNALVCRLAYELQKNLKIYPLVGFEHYAERWQTFDIFRDQPVKLVTEGNEIHGISRGINQQGELLLEQNGT
ITPFGIGEMSLRAE
>Mature_253_residues
SKLNQSQIQTALLCGQALVFDEIDSTNEYLLKHYQTLPQGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHYPTEDA
AHISSLSLVVSLIIAESLQAQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGIHLVIGIGLNLGMTKVDEKIVTQAWS
DLSDYQFDRNALVCRLAYELQKNLKIYPLVGFEHYAERWQTFDIFRDQPVKLVTEGNEIHGISRGINQQGELLLEQNGTI
TPFGIGEMSLRAE

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=253, Percent_Identity=43.8735177865613, Blast_Score=212, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24644246, Length=142, Percent_Identity=35.2112676056338, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28756; Mature: 28625

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLNQSQIQTALLCGQALVFDEIDSTNEYLLKHYQTLPQGSVCLAEQQTAGRGRRGRTW
CCCCCHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHCCCCCEEEECCHHCCCCCCCCCC
YSPQSENVYFSILWHYPTEDAAHISSLSLVVSLIIAESLQAQQVENIQIKWPNDIYYCGK
CCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEEECH
KMGGILIETKADRSGIHLVIGIGLNLGMTKVDEKIVTQAWSDLSDYQFDRNALVCRLAYE
HHCCEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
LQKNLKIYPLVGFEHYAERWQTFDIFRDQPVKLVTEGNEIHGISRGINQQGELLLEQNGT
HHHCCEEEEEECHHHHHHHHHEEHHHCCCCEEEEECCCEEEHHHHCCCCCCCEEEECCCC
ITPFGIGEMSLRAE
CCEECCCCEEEECC
>Mature Secondary Structure 
SKLNQSQIQTALLCGQALVFDEIDSTNEYLLKHYQTLPQGSVCLAEQQTAGRGRRGRTW
CCCCHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHCCCCCEEEECCHHCCCCCCCCCC
YSPQSENVYFSILWHYPTEDAAHISSLSLVVSLIIAESLQAQQVENIQIKWPNDIYYCGK
CCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEEECH
KMGGILIETKADRSGIHLVIGIGLNLGMTKVDEKIVTQAWSDLSDYQFDRNALVCRLAYE
HHCCEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
LQKNLKIYPLVGFEHYAERWQTFDIFRDQPVKLVTEGNEIHGISRGINQQGELLLEQNGT
HHHCCEEEEEECHHHHHHHHHEEHHHCCCCEEEEECCCEEEHHHHCCCCCCCEEEECCCC
ITPFGIGEMSLRAE
CCEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]