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The map label for this gene is spoT [H]

Identifier: 126209287

GI number: 126209287

Start: 2047341

End: 2049047

Strand: Reverse

Name: spoT [H]

Synonym: APL_1825

Alternate gene names: 126209287

Gene position: 2049047-2047341 (Counterclockwise)

Preceding gene: 126209288

Following gene: 126209286

Centisome position: 90.09

GC content: 46.46

Gene sequence:

>1707_bases
GTGTATCTTTTTCAACCGTTAGATAGCATTATTCAAGGCTATTTACCTGCTGATAAAATCGAGCTGGTAAAACGTGCCTT
TGTGATTGCGCGTGATGCGCACGAGGGGCAAACTCGCTCAAGCGGGGAGCCTTACATAACGCATCCGGTAGCAGTCGCAT
CCATTATTGCGGAAATGAAACTCGACCATGAGGCGGTCATGGCGGCATTGTTACACGATGTGATTGAAGATACGCCTTAC
ACGGAAGAGCAGCTTGCCGCCGAATTCGGTTCGAGCGTAGCGGAAATCGTGCAAGGCGTTTCCAAGCTGGATAAATTAAA
ATTCCGTACTCGCCAAGAAGCGCAAGTCGAGAATTTCCGCAAAATGATTTTGGCGATGACCAAAGACATTCGAGTGGTGT
TAATCAAGTTGGCCGACCGTACCCATAATATGCGTACGTTGGGTGCGTTACGTCCGGATAAGCGTCGCCGTATTGCCAAA
GAAACCCTTGAGATTTACAGTCCGTTAGCACATCGCTTAGGTATTGAACATCTTAAAAACGAATTGGAAGATCTTTGCTT
CCAAGCGATGCACCCTCATCGTTATCGTGTGTTACGTATGGCGATTGATATGGCGCGCGGTACTCGCCAAGACCTTATTT
CGACTATTTCCCACGAAATTCAATCACGTTTAGATGAGGCCGGTATTCAAGGTCGAGTTTACGGTCGAGAAAAACACCTC
TACTCACTCTATGAAAAAATGCGTCAGCGTGATCAGCATTTCCATTCCATTTTAGACATTTATGCGTTTAGAGTTGTGGT
GGGAAATGTTGATCATTGTTACCGTGCGTTAGGGCAAATGCACGCGCTCTATAAACCGCGTCCGTATAAAATCCGTGATT
ATATTGCCGTACCGAAAACCAACGGCTATCAATCTTTACACACGTCAATGATTGGGCATAAAGGCGTGCCGATCGAAGTA
CAGATTCGTACCGAAGATATGGATTTAATGGCGGAACTCGGCGTGGCGGCACATTGGCGTTATACCGAAGATCAAGCAAC
CGCAACCAGCGTACAGCAAAAAGCGCAACAATGGTTGCGTAGCATCGTCGAACTGCAACAAAGTGCCGGCAACTCGAATG
AATTTATCGAAAACGTGAAATCCGATTTCTTCTCGGATGATATTTACGTATTTACCCCGAAAGGACGTATTGTCGAATTA
CCGGCAAATGCGACCGCTATCGATTTTGCCTATGCGGTACATTCCGATATCGGTGATCGTTGCGTCGGTGCGATTGTCGA
TCGCAATCCGTATCCGATTTCACAACCGTTACAATCCGGTCAAACCGTTGAAATCATTACCAAGCAAGGTAAACGTCCAA
GCCCGTATTGGTTAAATTTCGCCGTCAGCTCAAGAGCCAAATCCAAAATTCGTGCGGCGCTTAAACATCTTGAAATAGAC
GAGCAAACGTCACAAGAGCCGGAAAAACCGGATCATTTGGATGTCGATATCGAACTGGAGATTAAAGACCAACCGGGAGT
ACTGGCAAGTCTAACCAATGCGATTGCTTCGATGAACAGCAATATCGGCACGGTGGAAAGCCGACCGAACGGTACCGGTA
ATTATCAGGTCAAAATGCGTATTGCCGTTACCGATAATGCACACCTTTATGTGGTGATTCAGAAGCTAACCAAAGTAAAC
GGTGTCGTTCGAGTCACGAAAGCCTAA

Upstream 100 bases:

>100_bases
CTTAAGATCAATTTTATTCAAAATTTAGATGGATAATTGAAAATAAATAGTAGTACTATCTATTGGCAATTATCCATTTT
TAATTATATCGAGGTGTACC

Downstream 100 bases:

>100_bases
TAACCTAAACATAAGCGGTGAATTTTTGTAAATTTTTTACAAAATTTGACCGCTTATTTTCTCGTGAATCCCTCTCAATT
CGGCTATAATTGCCCGAATT

Product: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (ppGpp)ase)

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MYLFQPLDSIIQGYLPADKIELVKRAFVIARDAHEGQTRSSGEPYITHPVAVASIIAEMKLDHEAVMAALLHDVIEDTPY
TEEQLAAEFGSSVAEIVQGVSKLDKLKFRTRQEAQVENFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
ETLEIYSPLAHRLGIEHLKNELEDLCFQAMHPHRYRVLRMAIDMARGTRQDLISTISHEIQSRLDEAGIQGRVYGREKHL
YSLYEKMRQRDQHFHSILDIYAFRVVVGNVDHCYRALGQMHALYKPRPYKIRDYIAVPKTNGYQSLHTSMIGHKGVPIEV
QIRTEDMDLMAELGVAAHWRYTEDQATATSVQQKAQQWLRSIVELQQSAGNSNEFIENVKSDFFSDDIYVFTPKGRIVEL
PANATAIDFAYAVHSDIGDRCVGAIVDRNPYPISQPLQSGQTVEIITKQGKRPSPYWLNFAVSSRAKSKIRAALKHLEID
EQTSQEPEKPDHLDVDIELEIKDQPGVLASLTNAIASMNSNIGTVESRPNGTGNYQVKMRIAVTDNAHLYVVIQKLTKVN
GVVRVTKA

Sequences:

>Translated_568_residues
MYLFQPLDSIIQGYLPADKIELVKRAFVIARDAHEGQTRSSGEPYITHPVAVASIIAEMKLDHEAVMAALLHDVIEDTPY
TEEQLAAEFGSSVAEIVQGVSKLDKLKFRTRQEAQVENFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
ETLEIYSPLAHRLGIEHLKNELEDLCFQAMHPHRYRVLRMAIDMARGTRQDLISTISHEIQSRLDEAGIQGRVYGREKHL
YSLYEKMRQRDQHFHSILDIYAFRVVVGNVDHCYRALGQMHALYKPRPYKIRDYIAVPKTNGYQSLHTSMIGHKGVPIEV
QIRTEDMDLMAELGVAAHWRYTEDQATATSVQQKAQQWLRSIVELQQSAGNSNEFIENVKSDFFSDDIYVFTPKGRIVEL
PANATAIDFAYAVHSDIGDRCVGAIVDRNPYPISQPLQSGQTVEIITKQGKRPSPYWLNFAVSSRAKSKIRAALKHLEID
EQTSQEPEKPDHLDVDIELEIKDQPGVLASLTNAIASMNSNIGTVESRPNGTGNYQVKMRIAVTDNAHLYVVIQKLTKVN
GVVRVTKA
>Mature_568_residues
MYLFQPLDSIIQGYLPADKIELVKRAFVIARDAHEGQTRSSGEPYITHPVAVASIIAEMKLDHEAVMAALLHDVIEDTPY
TEEQLAAEFGSSVAEIVQGVSKLDKLKFRTRQEAQVENFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
ETLEIYSPLAHRLGIEHLKNELEDLCFQAMHPHRYRVLRMAIDMARGTRQDLISTISHEIQSRLDEAGIQGRVYGREKHL
YSLYEKMRQRDQHFHSILDIYAFRVVVGNVDHCYRALGQMHALYKPRPYKIRDYIAVPKTNGYQSLHTSMIGHKGVPIEV
QIRTEDMDLMAELGVAAHWRYTEDQATATSVQQKAQQWLRSIVELQQSAGNSNEFIENVKSDFFSDDIYVFTPKGRIVEL
PANATAIDFAYAVHSDIGDRCVGAIVDRNPYPISQPLQSGQTVEIITKQGKRPSPYWLNFAVSSRAKSKIRAALKHLEID
EQTSQEPEKPDHLDVDIELEIKDQPGVLASLTNAIASMNSNIGTVESRPNGTGNYQVKMRIAVTDNAHLYVVIQKLTKVN
GVVRVTKA

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=484, Percent_Identity=69.4214876033058, Blast_Score=721, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=444, Percent_Identity=38.2882882882883, Blast_Score=325, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI25143118, Length=141, Percent_Identity=35.4609929078014, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 64384; Mature: 64384

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLFQPLDSIIQGYLPADKIELVKRAFVIARDAHEGQTRSSGEPYITHPVAVASIIAEMK
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHH
LDHEAVMAALLHDVIEDTPYTEEQLAAEFGSSVAEIVQGVSKLDKLKFRTRQEAQVENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAKETLEIYSPLAHRLGIEHLKN
HHHHHHHCCHHHHHEEHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
ELEDLCFQAMHPHRYRVLRMAIDMARGTRQDLISTISHEIQSRLDEAGIQGRVYGREKHL
HHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHH
YSLYEKMRQRDQHFHSILDIYAFRVVVGNVDHCYRALGQMHALYKPRPYKIRDYIAVPKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
NGYQSLHTSMIGHKGVPIEVQIRTEDMDLMAELGVAAHWRYTEDQATATSVQQKAQQWLR
CCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCHHHEEEECCHHHHHHHHHHHHHHHHH
SIVELQQSAGNSNEFIENVKSDFFSDDIYVFTPKGRIVELPANATAIDFAYAVHSDIGDR
HHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHCHHHH
CVGAIVDRNPYPISQPLQSGQTVEIITKQGKRPSPYWLNFAVSSRAKSKIRAALKHLEID
HHHHHHCCCCCCCCHHHHCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC
EQTSQEPEKPDHLDVDIELEIKDQPGVLASLTNAIASMNSNIGTVESRPNGTGNYQVKMR
CHHCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
IAVTDNAHLYVVIQKLTKVNGVVRVTKA
EEEECCCEEEEHHHHHHHCCCEEEEECC
>Mature Secondary Structure
MYLFQPLDSIIQGYLPADKIELVKRAFVIARDAHEGQTRSSGEPYITHPVAVASIIAEMK
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHH
LDHEAVMAALLHDVIEDTPYTEEQLAAEFGSSVAEIVQGVSKLDKLKFRTRQEAQVENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAKETLEIYSPLAHRLGIEHLKN
HHHHHHHCCHHHHHEEHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
ELEDLCFQAMHPHRYRVLRMAIDMARGTRQDLISTISHEIQSRLDEAGIQGRVYGREKHL
HHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHH
YSLYEKMRQRDQHFHSILDIYAFRVVVGNVDHCYRALGQMHALYKPRPYKIRDYIAVPKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
NGYQSLHTSMIGHKGVPIEVQIRTEDMDLMAELGVAAHWRYTEDQATATSVQQKAQQWLR
CCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCHHHEEEECCHHHHHHHHHHHHHHHHH
SIVELQQSAGNSNEFIENVKSDFFSDDIYVFTPKGRIVELPANATAIDFAYAVHSDIGDR
HHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHCHHHH
CVGAIVDRNPYPISQPLQSGQTVEIITKQGKRPSPYWLNFAVSSRAKSKIRAALKHLEID
HHHHHHCCCCCCCCHHHHCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC
EQTSQEPEKPDHLDVDIELEIKDQPGVLASLTNAIASMNSNIGTVESRPNGTGNYQVKMR
CHHCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
IAVTDNAHLYVVIQKLTKVNGVVRVTKA
EEEECCCEEEEHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]