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The map label for this gene is glyS

Identifier: 126209267

GI number: 126209267

Start: 2025782

End: 2027851

Strand: Direct

Name: glyS

Synonym: APL_1803

Alternate gene names: 126209267

Gene position: 2025782-2027851 (Clockwise)

Preceding gene: 126209266

Following gene: 126209268

Centisome position: 89.07

GC content: 45.65

Gene sequence:

>2070_bases
ATGACAACACAAAACTTCCTCGCCGAGATCGGCACTGAAGAGTTGCCACCGAAGGCACTCAAAAAATTAGCGACCGCATT
TGCGGAGAATGTAGAAAACGAGTTAAACCAAGCGGATTTATCATTCGAGAAAGTAGAATGGTTTGCAGCACCTCGTCGTT
TGGCGGTGAAAGCGTTAGGTTTGGCGACCGCTCAACCAAGCAAAGAAATCGAGAAACGTGGTCCTGCGGTGTCGGCGGCG
TTTGACGCAGACGGCAAGCCAACCAAAGCGGCGGAAGGCTGGGCGAGAGGTTGTGGTATTACCGTTGATCAAGCGGATCG
AATCGCTACCGATAAAGGCGAATGGTTAGTTCACCGTGCGGTAATTGAAGGTCAGCCGACCAAAAATTTATTGGTGGACA
TTATTTCTCGTTCTTTAGCGAATTTACCGATTCCAAAAATGATGCGTTGGGGCGATAAAACCGAGCAATTCGTGCGCCCT
GTGCATACGGTTACCTTATTCTTTGGTAGTGAGCTAATCGAAGGCGAAATTTTAGGCGTGAAAATCAATAATGTAATTCG
TGGACACCGTTTCTTAGGCGAGCGTGAATTTACTATTTCACACGCAGACGAATACCTTACTGCATTACGTGAAAAAGGTA
TGGTGGTTGCCGACTTCAACGAACGTAAAGCGTTGATTCTTGCAAAATCGCAAGAAAAAGCGACCGCTTTAGGTGGCGTG
GCAGACATCGAAGAAGACTTATTAGATGAAGTGACTTCATTGGTTGAGTTCCCGAACGTATTAACCGCAAAATTTGAAGA
GCGTTTCCTTGCTGTGCCTGCGGAAGCATTGGTTTACACAATGAAAGGCGACCAAAAATACTTCCCGATTTATGACAAAG
ATGGCAAATTATTACCGCACTTTATCTTTGTGTCGAACATTAATCCGGAAGATCCGACAGCGATTATCGAAGGTAATGAA
AAAGTGGTTCGTCCACGTTTAACCGATGCGGAATTCTTCTTCAAAACCGACTTAAAACAGCGTTTAGAAGACCGTTTACC
ACGCCTTGAAACCGTGTTGTTCCAACAACAGTTAGGTACGTTACGTGATAAAACCGCTCGTATTGAAGCATTAGCGGGCG
AAATCGCTGCACAAATCGGTGCGGACAAAGCGAAAGCAGAGCGTGCGGGCTTACTTTCTAAATGCGACTTAATGACCAAT
ATGGTGTTTGAATTTACCGACACGCAAGGCGTGATGGGTATGCACTATGCTCGTCATGACGGCGAAGATGAAGAAGTCGC
AGTGGCATTGAACGAGCAATATATGCCACGTTTCGCCGGAGATGAATTACCGAAATCATTAGTCGCTTGTTCAGTAGCAT
TAGCGGACAAATTCGACACGCTCACGGGGATTTTCGGTATCGGTCAAGCACCGAAAGGCAGTGCCGACCCATTCGCACTA
CGTCGTGCGGCATTAGGTTCGCTCCGTATTATCGTGGAGAAAAATTTACCGCTTGATTTAGAAGATTTAGTGCGTAAATC
GGCTGCATTATTCGGTAATAAATTGACCAATGCAAATGTAGTTGAAGATGTAGTGGACTTTATGCTCGGTCGTTTCCGTG
CGTGGTATCAAGACGAAGGTATTGCGGTGGACGTTATCCAAGCGGTATTGGCTCGCCGTCCAACTAAGCCGGCTGACTTT
GACGCTCGTGTGCGTGCGGTATCGCACTTCCGTACCTTAGATTCAGCGGAAGCCTTAGCCGCAGCGAACAAACGCGTAAG
CAATATTTTAGCGAAAGTGGAAGGCGAAATTTCAATCGAAATCGACCGCACTTTATTAGTTGAAGCGGAAGAAAAAGCCC
TTGCAGAACAGGTAATTAGCTTACAAACGGAATTAGCTCCGACATTTGCAAACGGCGAATATCAAACCGCACTTGATCGC
TTAGCGAGTTTACGTGAAACGGTGGATAACTTCTTTGAAAAAGTGATGGTAAACGCAGAAGATGCAAATCTTCGCCGTAA
CCGTCAGGCAATTCTAAATAATTTACGTAACTTGTTCTTACAAGTAGCGGATATTTCCGTTTTACAATAA

Upstream 100 bases:

>100_bases
CAGAAGAAAATAAGTGATTTAAATTTTGGACACGGTGCAGAAAAAACATATTTTGAAATTTAATGGTGCAATAAATTGCA
CTCTAGCAGAGAGAAATACA

Downstream 100 bases:

>100_bases
TTTGTTGAAATTAGACCGCTTTTTTAGGCGGTCTATTTTTAAGCGAAATAAGTTAGATTAATCACTAAGTCATTGATTAA
ATTTATAGCAAACACAACAA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MTTQNFLAEIGTEELPPKALKKLATAFAENVENELNQADLSFEKVEWFAAPRRLAVKALGLATAQPSKEIEKRGPAVSAA
FDADGKPTKAAEGWARGCGITVDQADRIATDKGEWLVHRAVIEGQPTKNLLVDIISRSLANLPIPKMMRWGDKTEQFVRP
VHTVTLFFGSELIEGEILGVKINNVIRGHRFLGEREFTISHADEYLTALREKGMVVADFNERKALILAKSQEKATALGGV
ADIEEDLLDEVTSLVEFPNVLTAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPHFIFVSNINPEDPTAIIEGNE
KVVRPRLTDAEFFFKTDLKQRLEDRLPRLETVLFQQQLGTLRDKTARIEALAGEIAAQIGADKAKAERAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVACSVALADKFDTLTGIFGIGQAPKGSADPFAL
RRAALGSLRIIVEKNLPLDLEDLVRKSAALFGNKLTNANVVEDVVDFMLGRFRAWYQDEGIAVDVIQAVLARRPTKPADF
DARVRAVSHFRTLDSAEALAAANKRVSNILAKVEGEISIEIDRTLLVEAEEKALAEQVISLQTELAPTFANGEYQTALDR
LASLRETVDNFFEKVMVNAEDANLRRNRQAILNNLRNLFLQVADISVLQ

Sequences:

>Translated_689_residues
MTTQNFLAEIGTEELPPKALKKLATAFAENVENELNQADLSFEKVEWFAAPRRLAVKALGLATAQPSKEIEKRGPAVSAA
FDADGKPTKAAEGWARGCGITVDQADRIATDKGEWLVHRAVIEGQPTKNLLVDIISRSLANLPIPKMMRWGDKTEQFVRP
VHTVTLFFGSELIEGEILGVKINNVIRGHRFLGEREFTISHADEYLTALREKGMVVADFNERKALILAKSQEKATALGGV
ADIEEDLLDEVTSLVEFPNVLTAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPHFIFVSNINPEDPTAIIEGNE
KVVRPRLTDAEFFFKTDLKQRLEDRLPRLETVLFQQQLGTLRDKTARIEALAGEIAAQIGADKAKAERAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVACSVALADKFDTLTGIFGIGQAPKGSADPFAL
RRAALGSLRIIVEKNLPLDLEDLVRKSAALFGNKLTNANVVEDVVDFMLGRFRAWYQDEGIAVDVIQAVLARRPTKPADF
DARVRAVSHFRTLDSAEALAAANKRVSNILAKVEGEISIEIDRTLLVEAEEKALAEQVISLQTELAPTFANGEYQTALDR
LASLRETVDNFFEKVMVNAEDANLRRNRQAILNNLRNLFLQVADISVLQ
>Mature_688_residues
TTQNFLAEIGTEELPPKALKKLATAFAENVENELNQADLSFEKVEWFAAPRRLAVKALGLATAQPSKEIEKRGPAVSAAF
DADGKPTKAAEGWARGCGITVDQADRIATDKGEWLVHRAVIEGQPTKNLLVDIISRSLANLPIPKMMRWGDKTEQFVRPV
HTVTLFFGSELIEGEILGVKINNVIRGHRFLGEREFTISHADEYLTALREKGMVVADFNERKALILAKSQEKATALGGVA
DIEEDLLDEVTSLVEFPNVLTAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPHFIFVSNINPEDPTAIIEGNEK
VVRPRLTDAEFFFKTDLKQRLEDRLPRLETVLFQQQLGTLRDKTARIEALAGEIAAQIGADKAKAERAGLLSKCDLMTNM
VFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVACSVALADKFDTLTGIFGIGQAPKGSADPFALR
RAALGSLRIIVEKNLPLDLEDLVRKSAALFGNKLTNANVVEDVVDFMLGRFRAWYQDEGIAVDVIQAVLARRPTKPADFD
ARVRAVSHFRTLDSAEALAAANKRVSNILAKVEGEISIEIDRTLLVEAEEKALAEQVISLQTELAPTFANGEYQTALDRL
ASLRETVDNFFEKVMVNAEDANLRRNRQAILNNLRNLFLQVADISVLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=69.2307692307692, Blast_Score=959, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_ACTP2 (A3N3A1)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054492.1
- ProteinModelPortal:   A3N3A1
- STRING:   A3N3A1
- GeneID:   4848617
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1803
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   A3N3A1
- ProtClustDB:   PRK01233
- BioCyc:   APLE416269:APL_1803-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 76380; Mature: 76249

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQNFLAEIGTEELPPKALKKLATAFAENVENELNQADLSFEKVEWFAAPRRLAVKALG
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHH
LATAQPSKEIEKRGPAVSAAFDADGKPTKAAEGWARGCGITVDQADRIATDKGEWLVHRA
HCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCEECCCHHHCCCCCCEEEEEH
VIEGQPTKNLLVDIISRSLANLPIPKMMRWGDKTEQFVRPVHTVTLFFGSELIEGEILGV
HHCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCHHHCCCEEEE
KINNVIRGHRFLGEREFTISHADEYLTALREKGMVVADFNERKALILAKSQEKATALGGV
EHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCHHHHHHCCH
ADIEEDLLDEVTSLVEFPNVLTAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHEEEEECCCCEECCEECCCCCCCCE
FIFVSNINPEDPTAIIEGNEKVVRPRLTDAEFFFKTDLKQRLEDRLPRLETVLFQQQLGT
EEEEECCCCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDKTARIEALAGEIAAQIGADKAKAERAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCC
GEDEEVAVALNEQYMPRFAGDELPKSLVACSVALADKFDTLTGIFGIGQAPKGSADPFAL
CCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGSLRIIVEKNLPLDLEDLVRKSAALFGNKLTNANVVEDVVDFMLGRFRAWYQDEG
HHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
IAVDVIQAVLARRPTKPADFDARVRAVSHFRTLDSAEALAAANKRVSNILAKVEGEISIE
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IDRTLLVEAEEKALAEQVISLQTELAPTFANGEYQTALDRLASLRETVDNFFEKVMVNAE
ECCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
DANLRRNRQAILNNLRNLFLQVADISVLQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQNFLAEIGTEELPPKALKKLATAFAENVENELNQADLSFEKVEWFAAPRRLAVKALG
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHH
LATAQPSKEIEKRGPAVSAAFDADGKPTKAAEGWARGCGITVDQADRIATDKGEWLVHRA
HCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCEECCCHHHCCCCCCEEEEEH
VIEGQPTKNLLVDIISRSLANLPIPKMMRWGDKTEQFVRPVHTVTLFFGSELIEGEILGV
HHCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCHHHCCCEEEE
KINNVIRGHRFLGEREFTISHADEYLTALREKGMVVADFNERKALILAKSQEKATALGGV
EHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCHHHHHHCCH
ADIEEDLLDEVTSLVEFPNVLTAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHEEEEECCCCEECCEECCCCCCCCE
FIFVSNINPEDPTAIIEGNEKVVRPRLTDAEFFFKTDLKQRLEDRLPRLETVLFQQQLGT
EEEEECCCCCCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDKTARIEALAGEIAAQIGADKAKAERAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCC
GEDEEVAVALNEQYMPRFAGDELPKSLVACSVALADKFDTLTGIFGIGQAPKGSADPFAL
CCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGSLRIIVEKNLPLDLEDLVRKSAALFGNKLTNANVVEDVVDFMLGRFRAWYQDEG
HHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
IAVDVIQAVLARRPTKPADFDARVRAVSHFRTLDSAEALAAANKRVSNILAKVEGEISIE
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IDRTLLVEAEEKALAEQVISLQTELAPTFANGEYQTALDRLASLRETVDNFFEKVMVNAE
ECCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
DANLRRNRQAILNNLRNLFLQVADISVLQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA