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The map label for this gene is rpoA

Identifier: 126209248

GI number: 126209248

Start: 2009118

End: 2010107

Strand: Direct

Name: rpoA

Synonym: APL_1784

Alternate gene names: 126209248

Gene position: 2009118-2010107 (Clockwise)

Preceding gene: 126209247

Following gene: 126209249

Centisome position: 88.33

GC content: 42.02

Gene sequence:

>990_bases
ATGCAGGGTTCTGTGACAGAATTTTTAAAGCCGCACTTAGTGAATATCGAACAAGTTAGTTCTACTCACGCAAAAGTGAC
CTTAGAACCGTTAGAACGTGGTTTTGGCCATACATTAGGTAACGCACTTCGTCGCATTTTACTTTCGTCTATGCCGGGTT
GTGCCGTTACAGAAGTTGAAATTGATGGTGTATTGCATGAATACAGCAGCAAAGAAGGCGTACAAGAAGATATTCTTGAA
GTATTGTTAAACCTTAAAGGTCTAGCGGTAAAAGTGTCGGGTAAAGATGATATTATTTTGACACTAAATAAGTCTGGAAT
TGGCCCTGTAACTGCAGCCGACATTACGCATGATGGTGACGTAGAAATTGTAAATCCACACCATGTTATCTGCCACTTAA
CAGATAAAGACGCATCAATTAGTATGCGTATTCGTGTTCAGCGTGGTCGTGGTTATGTACCGGCATCTGCTCGCGTACAT
TCTCAAGATGAAGATCGTCCAATCGGTCGTTTATTAGTTGATGCTCGTTTCAGCCCAGTAGATCGCATTGCTTATAATGT
GGAAAGTGCGCGTGTTGAACAGCGTACTGACTTAGATAAACTCATCATTGAGATGGAAACAAATGGCACAATCGATCCAG
AAGAAGCCATTCGTCGCGCTGCTACAATTTTAGCAGAACAGTTAGATGCATTCGTTGATTTACGTGATGTTCGTCAGCCT
GAAGTGAAAGAAGAAAAACCGGAGTTCGATCCGATTTTACTTCGTCCGGTAGATGATTTAGAGCTGACAGTTCGTTCGCA
TAACTGTTTGAAAGCAGAGACAATTCACTATATCTGTGATTTAGTACAACGTACAGAAGTCGAGTTATTGAAAACGCCTA
ATCTTGGTAAGAAATCACTTACTGAGATTAAGGATGTGCTTGCTTCTCGCGGCTTATCACTGGGTATGCGCCTTGAGAAT
TGGCCTCCGGCAAGTATTGCCGAAGACTAA

Upstream 100 bases:

>100_bases
ACGAACATCTGATCGTTGAGCTTTACTCTAAATAATAGTTAATTTTTAAAATTACATTATTAGATTACTAAATAAAGCTT
AAAAACAAAGAGAGGATAAA

Downstream 100 bases:

>100_bases
TTTTAGGTATTAGATTTTCTAAGAAGGAATAGGTTATGCGCCATCGTAAGAGTGGACGTCAATTAAACCGTAACAGCAGC
CATCGCCAAGCGATGTTCCG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILE
VLLNLKGLAVKVSGKDDIILTLNKSGIGPVTAADITHDGDVEIVNPHHVICHLTDKDASISMRIRVQRGRGYVPASARVH
SQDEDRPIGRLLVDARFSPVDRIAYNVESARVEQRTDLDKLIIEMETNGTIDPEEAIRRAATILAEQLDAFVDLRDVRQP
EVKEEKPEFDPILLRPVDDLELTVRSHNCLKAETIHYICDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLEN
WPPASIAED

Sequences:

>Translated_329_residues
MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILE
VLLNLKGLAVKVSGKDDIILTLNKSGIGPVTAADITHDGDVEIVNPHHVICHLTDKDASISMRIRVQRGRGYVPASARVH
SQDEDRPIGRLLVDARFSPVDRIAYNVESARVEQRTDLDKLIIEMETNGTIDPEEAIRRAATILAEQLDAFVDLRDVRQP
EVKEEKPEFDPILLRPVDDLELTVRSHNCLKAETIHYICDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLEN
WPPASIAED
>Mature_329_residues
MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILE
VLLNLKGLAVKVSGKDDIILTLNKSGIGPVTAADITHDGDVEIVNPHHVICHLTDKDASISMRIRVQRGRGYVPASARVH
SQDEDRPIGRLLVDARFSPVDRIAYNVESARVEQRTDLDKLIIEMETNGTIDPEEAIRRAATILAEQLDAFVDLRDVRQP
EVKEEKPEFDPILLRPVDDLELTVRSHNCLKAETIHYICDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLEN
WPPASIAED

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=329, Percent_Identity=90.8814589665653, Blast_Score=614, Evalue=1e-177,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_ACTP2 (A3N382)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054473.1
- ProteinModelPortal:   A3N382
- STRING:   A3N382
- GeneID:   4849580
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1784
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   A3N382
- ProtClustDB:   PRK05182
- BioCyc:   APLE416269:APL_1784-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 36540; Mature: 36540

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
CCCCHHHHHCCHHCCHHHHCCCCCEEEHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEE
IDGVLHEYSSKEGVQEDILEVLLNLKGLAVKVSGKDDIILTLNKSGIGPVTAADITHDGD
HHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCEEEECCCCCCC
VEIVNPHHVICHLTDKDASISMRIRVQRGRGYVPASARVHSQDEDRPIGRLLVDARFSPV
EEEECCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCEECCCCCCCCHHHEEEECCCCCH
DRIAYNVESARVEQRTDLDKLIIEMETNGTIDPEEAIRRAATILAEQLDAFVDLRDVRQP
HHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EVKEEKPEFDPILLRPVDDLELTVRSHNCLKAETIHYICDLVQRTEVELLKTPNLGKKSL
CCCCCCCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHH
TEIKDVLASRGLSLGMRLENWPPASIAED
HHHHHHHHHCCCEECCEECCCCCCCCCCC
>Mature Secondary Structure
MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE
CCCCHHHHHCCHHCCHHHHCCCCCEEEHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEE
IDGVLHEYSSKEGVQEDILEVLLNLKGLAVKVSGKDDIILTLNKSGIGPVTAADITHDGD
HHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCEEEECCCCCCC
VEIVNPHHVICHLTDKDASISMRIRVQRGRGYVPASARVHSQDEDRPIGRLLVDARFSPV
EEEECCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCEECCCCCCCCHHHEEEECCCCCH
DRIAYNVESARVEQRTDLDKLIIEMETNGTIDPEEAIRRAATILAEQLDAFVDLRDVRQP
HHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EVKEEKPEFDPILLRPVDDLELTVRSHNCLKAETIHYICDLVQRTEVELLKTPNLGKKSL
CCCCCCCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHH
TEIKDVLASRGLSLGMRLENWPPASIAED
HHHHHHHHHCCCEECCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA