Click here to switch to the map view.

The map label for this gene is nanK [H]

Identifier: 126209217

GI number: 126209217

Start: 1990190

End: 1991062

Strand: Direct

Name: nanK [H]

Synonym: APL_1753

Alternate gene names: 126209217

Gene position: 1990190-1991062 (Clockwise)

Preceding gene: 126209216

Following gene: 126209218

Centisome position: 87.5

GC content: 48.34

Gene sequence:

>873_bases
ATGCGTTGTTTAGCATTAGATATCGGCGGTACTAAGATTGCCTCGGCATTAGTCGAAAACGGAGTGATTTCCCAACGCCG
ACAAATCGGCACACCGCAACAAGATGCGGCGGAAGCAATGCATCAAACGTTGGCGGACATTTTGCAACAGTACCAAGGGC
AGTTTGGTGCGGTATCGGTAGCTTCGACCGGTATTATTAATAACGGCGTTTTAACCGCATTAAACCCGAAGAATTTAGGC
GGGTTGGCTTTTTTCCCGTTGCAAGAAAGTATTGCACGTCATACCGATAAACCGATTTTCTTACTCAATGACGTACAAGC
GGCGGCTTGTGCCGAATATCAACATCAAGATAAACAAGCGGTCGAAAATTTCGTCTTTATTACCGTTTCAACCGGGGTTG
GCGGCGGCATTATCCAAAATGGCAAATTGCTAACCCAACCGAATGGTGTAGCAGGACATATCGGGCATACTTTAGCGGAT
CCGAACGGGCCAATTTGTGGCTGTGGTCGCCGAGGTTGTGTGGAAGCGATTGCTTCCGGGCGAGCGATTGAAGCGGTTTC
AAGCCGATGGACGGAACCTTGTACACCGAAACAAGTGTTTGAGCAATTCCGAGCGGGCAAAGTGCAAGCGGTCGAATTAG
TCGAAAAATCTGCAAAAGCCATTGCCAATTTAGTGGCGGATTTAACCATCGGTTTAGATACGCAAAAAGTGGTGATCGGC
GGCAGTGTCGGCTTGGCGGAAGGTTATTTACCGCTCGTGCAAAAATATTTAGCCGAAATGCCGCATTTCTATCATTGTGA
ATTGGAAGCGGCGAAATATGGCGGCGATGCCGGTTTAATCGGTGCGGCAGCATGGGCGGAACAACATTTTTAA

Upstream 100 bases:

>100_bases
TGCGGATTGTGTTACGGTCGGTTCGGCACTCACTCGCTTGGAGCATATTGTCGGCTGGTTTGCTGATGAGATAAAAACTG
CGAAAGTTTAAGGGGAATGT

Downstream 100 bases:

>100_bases
TTAAATTTAAGGAACATAATATGAAAAACTTAACCGGTATTTTTAGTGCGTTACTCGTAGCATTTAACGAAGACGGTTCG
ATTAATGAGCAAGGCTTACG

Product: N-acetylmannosamine kinase

Products: NA

Alternate protein names: ManNAc kinase; N-acetyl-D-mannosamine kinase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRCLALDIGGTKIASALVENGVISQRRQIGTPQQDAAEAMHQTLADILQQYQGQFGAVSVASTGIINNGVLTALNPKNLG
GLAFFPLQESIARHTDKPIFLLNDVQAAACAEYQHQDKQAVENFVFITVSTGVGGGIIQNGKLLTQPNGVAGHIGHTLAD
PNGPICGCGRRGCVEAIASGRAIEAVSSRWTEPCTPKQVFEQFRAGKVQAVELVEKSAKAIANLVADLTIGLDTQKVVIG
GSVGLAEGYLPLVQKYLAEMPHFYHCELEAAKYGGDAGLIGAAAWAEQHF

Sequences:

>Translated_290_residues
MRCLALDIGGTKIASALVENGVISQRRQIGTPQQDAAEAMHQTLADILQQYQGQFGAVSVASTGIINNGVLTALNPKNLG
GLAFFPLQESIARHTDKPIFLLNDVQAAACAEYQHQDKQAVENFVFITVSTGVGGGIIQNGKLLTQPNGVAGHIGHTLAD
PNGPICGCGRRGCVEAIASGRAIEAVSSRWTEPCTPKQVFEQFRAGKVQAVELVEKSAKAIANLVADLTIGLDTQKVVIG
GSVGLAEGYLPLVQKYLAEMPHFYHCELEAAKYGGDAGLIGAAAWAEQHF
>Mature_290_residues
MRCLALDIGGTKIASALVENGVISQRRQIGTPQQDAAEAMHQTLADILQQYQGQFGAVSVASTGIINNGVLTALNPKNLG
GLAFFPLQESIARHTDKPIFLLNDVQAAACAEYQHQDKQAVENFVFITVSTGVGGGIIQNGKLLTQPNGVAGHIGHTLAD
PNGPICGCGRRGCVEAIASGRAIEAVSSRWTEPCTPKQVFEQFRAGKVQAVELVEKSAKAIANLVADLTIGLDTQKVVIG
GSVGLAEGYLPLVQKYLAEMPHFYHCELEAAKYGGDAGLIGAAAWAEQHF

Specific function: Catalyzes the phosphorylation of N-acetylmannosamine (ManNAc) to ManNAc-6-P [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NanK subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082230, Length=277, Percent_Identity=51.2635379061372, Blast_Score=244, Evalue=4e-66,
Organism=Escherichia coli, GI1787363, Length=301, Percent_Identity=27.2425249169435, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786891, Length=236, Percent_Identity=27.1186440677966, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787878, Length=213, Percent_Identity=25.3521126760563, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081733, Length=306, Percent_Identity=24.8366013071895, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87082127, Length=164, Percent_Identity=28.6585365853659, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.60 [H]

Molecular weight: Translated: 30587; Mature: 30587

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCLALDIGGTKIASALVENGVISQRRQIGTPQQDAAEAMHQTLADILQQYQGQFGAVSV
CEEEEEECCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
ASTGIINNGVLTALNPKNLGGLAFFPLQESIARHTDKPIFLLNDVQAAACAEYQHQDKQA
HHCCEECCCEEEEECCCCCCCEEEECHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
VENFVFITVSTGVGGGIIQNGKLLTQPNGVAGHIGHTLADPNGPICGCGRRGCVEAIASG
HCCEEEEEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHCC
RAIEAVSSRWTEPCTPKQVFEQFRAGKVQAVELVEKSAKAIANLVADLTIGLDTQKVVIG
CHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
GSVGLAEGYLPLVQKYLAEMPHFYHCELEAAKYGGDAGLIGAAAWAEQHF
CCCCHHHCCHHHHHHHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MRCLALDIGGTKIASALVENGVISQRRQIGTPQQDAAEAMHQTLADILQQYQGQFGAVSV
CEEEEEECCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
ASTGIINNGVLTALNPKNLGGLAFFPLQESIARHTDKPIFLLNDVQAAACAEYQHQDKQA
HHCCEECCCEEEEECCCCCCCEEEECHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
VENFVFITVSTGVGGGIIQNGKLLTQPNGVAGHIGHTLADPNGPICGCGRRGCVEAIASG
HCCEEEEEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHCC
RAIEAVSSRWTEPCTPKQVFEQFRAGKVQAVELVEKSAKAIANLVADLTIGLDTQKVVIG
CHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
GSVGLAEGYLPLVQKYLAEMPHFYHCELEAAKYGGDAGLIGAAAWAEQHF
CCCCHHHCCHHHHHHHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]