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The map label for this gene is yaeR [H]

Identifier: 126209202

GI number: 126209202

Start: 1973932

End: 1974324

Strand: Reverse

Name: yaeR [H]

Synonym: APL_1738

Alternate gene names: 126209202

Gene position: 1974324-1973932 (Counterclockwise)

Preceding gene: 126209207

Following gene: 126209201

Centisome position: 86.8

GC content: 42.24

Gene sequence:

>393_bases
ATGCTAACACCCATTCTCGGCTTTCATCATGTGGCGATTATTGCCGGTGATTATGCCAAATCCAAACATTTTTATACGCA
AATTCTCGGTGCGGAAATTATTGAAGAAACTTACCGTGCGGCACGTGAAAGCTATAAGCTCGATCTTCGTTTTGCCGACG
GTTCGCAAATTGAATTATTTAGTTTTCCGAATCCGCCACAACGCCCAAATAGTCCGGAGGCCTGTGGCTTGCGCCATTTA
GCGTTTCGTGTAAAAGATGTGCAACAAGCGGTCGAATTTTTAGCAAAAAATGCAGTGAAATGTGAACCGATTCGAATTGA
TGATTTGACCGGTAAACGCTTCACTTTCTTTAAAGATCCGGACGGTTTACCGCTAGAGTTTTATGAAATTTAA

Upstream 100 bases:

>100_bases
CACTCGAAGCCTTTTAATATCAAAATAATATCAAGCCGTGCATCATAACCTAAGCGGTCATATTTAACAGTTTTTTTGCA
AAACTTTTACAAGGAAATTT

Downstream 100 bases:

>100_bases
GGGTTGAAAATTTAAGCAATTCGACTTATAGTAAGAACACGGTGAGCAAACAAGTAATAACACTAAAAAGATAATGATAA
GCCAAAATGCTTTCATTTCT

Product: lactoylglutathione lyase and related lyases

Products: glutathione; methylglyoxal

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MLTPILGFHHVAIIAGDYAKSKHFYTQILGAEIIEETYRAARESYKLDLRFADGSQIELFSFPNPPQRPNSPEACGLRHL
AFRVKDVQQAVEFLAKNAVKCEPIRIDDLTGKRFTFFKDPDGLPLEFYEI

Sequences:

>Translated_130_residues
MLTPILGFHHVAIIAGDYAKSKHFYTQILGAEIIEETYRAARESYKLDLRFADGSQIELFSFPNPPQRPNSPEACGLRHL
AFRVKDVQQAVEFLAKNAVKCEPIRIDDLTGKRFTFFKDPDGLPLEFYEI
>Mature_130_residues
MLTPILGFHHVAIIAGDYAKSKHFYTQILGAEIIEETYRAARESYKLDLRFADGSQIELFSFPNPPQRPNSPEACGLRHL
AFRVKDVQQAVEFLAKNAVKCEPIRIDDLTGKRFTFFKDPDGLPLEFYEI

Specific function: Unknown

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis ywkD [H]

Homologues:

Organism=Escherichia coli, GI87081697, Length=121, Percent_Identity=62.8099173553719, Blast_Score=154, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5

Molecular weight: Translated: 14861; Mature: 14861

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPILGFHHVAIIAGDYAKSKHFYTQILGAEIIEETYRAARESYKLDLRFADGSQIELF
CCCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEE
SFPNPPQRPNSPEACGLRHLAFRVKDVQQAVEFLAKNAVKCEPIRIDDLTGKRFTFFKDP
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECC
DGLPLEFYEI
CCCCEEEEEC
>Mature Secondary Structure
MLTPILGFHHVAIIAGDYAKSKHFYTQILGAEIIEETYRAARESYKLDLRFADGSQIELF
CCCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEE
SFPNPPQRPNSPEACGLRHLAFRVKDVQQAVEFLAKNAVKCEPIRIDDLTGKRFTFFKDP
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECC
DGLPLEFYEI
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9723924; 9278503 [H]