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The map label for this gene is ulaG [H]

Identifier: 126209165

GI number: 126209165

Start: 1933388

End: 1934479

Strand: Direct

Name: ulaG [H]

Synonym: APL_1701

Alternate gene names: 126209165

Gene position: 1933388-1934479 (Clockwise)

Preceding gene: 126209160

Following gene: 126209166

Centisome position: 85.0

GC content: 42.86

Gene sequence:

>1092_bases
ATGGCTAAAGTTAATGAAATCACACGTGAAAGTTGGATTCTTTCTACCTTCCCTGAATGGGGCACTTGGCTAAACGAAGA
AATTGAGCAAGAAGTTGTACCGGAAGGTAACTTCGCAATGTGGTGGCTGGGTTGTGTCGGTGTGTGGATTAAAACGCCTG
CCGGTGCAAATATTTGTATGGATTTATGGTGTAGCCGTGGTAAATCCACTAAAAAAGTAAAAGATATGGTACGCGGTCAC
CAAATGGCGAATATGGCGGGCGTACGCAAATTACAACCGAACTTACGTGCCCAACCGATGGTATTGGATCCGTTTGCGAT
TAACGAAGTCGATTTTATTCTCGCTTCCCATTATCATAGCGACCATATTGATGTGAATGTGGCGGCGGCGATTGTAAACA
ACCCTAATTTAAATCATGTGAAATTCGTCGGTCCGTGGCACTGCGGCGAATTATGGAAAAAATGGGGCGTGCCGGAAGAT
CGTATCATCGTAGTAAAACCAGGAGACGTAGTAAAAATTAAAGACGTAGAAATTCATGCGTTAGATTCGTTTGACCGTAC
TTGTTTAGTAACTCTACCGGTTGAAGGGGCGGAAGAACAAGGCGGAGAATTAAAAGGTTTATGCCCGTCTGACGAAGAAA
TGGGACGTAAAGCGGTAAATTACGTATTTAAAACACCGGGCGGTAATATTTACCACGGTGCGGACTCTCACTACTCAATC
CAATTTGCGAAACACGGTAAAGAATTCGATATTGACGTGGCATTAAATAACTACGGTGAAAACCCGGTCGGTATCGCAGA
TAAAATGACCTCTATCGACTTACTACGTATGGCGGAATGTTTACGTACCAACGTGATTATTCCGGTACACCATGATATTT
GGACGAACTTTATGGCAAGTACGCAAGAAATTATTGATCTATGGCGTATGCGTAAAGATCGCTTACAATATAAATTCCAT
CCGTTTATTTGGGAAGTCGGTGGCAAATACGTTTATCCGCGCGATAAAGACTTAATTGAATATCACCACCCGCGCGGCTT
TGACGATTGCTTCGAGCAAGAGCCGAATATTCAATTTAAATCAATGCTTTAA

Upstream 100 bases:

>100_bases
ACATTTTTCACAAAAATCATTTTACCAACGTCGAATGATTACTTATGATTACTCTTGATTAAAATATTCATCTCTTCCTT
CCAAAAATTAGGAGTTTACT

Downstream 100 bases:

>100_bases
TTGAAGGCGAGCCACTCTTAACGGAATTTTAAGAGTGGCTTATTTTTCAGTTCATTGTTCATGGTTTAAAATGGATGACT
TATATAAGCAAGGACATGGT

Product: putative L-ascorbate 6-phosphate lactonase

Products: NA

Alternate protein names: L-ascorbate utilization protein G [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAKVNEITRESWILSTFPEWGTWLNEEIEQEVVPEGNFAMWWLGCVGVWIKTPAGANICMDLWCSRGKSTKKVKDMVRGH
QMANMAGVRKLQPNLRAQPMVLDPFAINEVDFILASHYHSDHIDVNVAAAIVNNPNLNHVKFVGPWHCGELWKKWGVPED
RIIVVKPGDVVKIKDVEIHALDSFDRTCLVTLPVEGAEEQGGELKGLCPSDEEMGRKAVNYVFKTPGGNIYHGADSHYSI
QFAKHGKEFDIDVALNNYGENPVGIADKMTSIDLLRMAECLRTNVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYKFH
PFIWEVGGKYVYPRDKDLIEYHHPRGFDDCFEQEPNIQFKSML

Sequences:

>Translated_363_residues
MAKVNEITRESWILSTFPEWGTWLNEEIEQEVVPEGNFAMWWLGCVGVWIKTPAGANICMDLWCSRGKSTKKVKDMVRGH
QMANMAGVRKLQPNLRAQPMVLDPFAINEVDFILASHYHSDHIDVNVAAAIVNNPNLNHVKFVGPWHCGELWKKWGVPED
RIIVVKPGDVVKIKDVEIHALDSFDRTCLVTLPVEGAEEQGGELKGLCPSDEEMGRKAVNYVFKTPGGNIYHGADSHYSI
QFAKHGKEFDIDVALNNYGENPVGIADKMTSIDLLRMAECLRTNVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYKFH
PFIWEVGGKYVYPRDKDLIEYHHPRGFDDCFEQEPNIQFKSML
>Mature_362_residues
AKVNEITRESWILSTFPEWGTWLNEEIEQEVVPEGNFAMWWLGCVGVWIKTPAGANICMDLWCSRGKSTKKVKDMVRGHQ
MANMAGVRKLQPNLRAQPMVLDPFAINEVDFILASHYHSDHIDVNVAAAIVNNPNLNHVKFVGPWHCGELWKKWGVPEDR
IIVVKPGDVVKIKDVEIHALDSFDRTCLVTLPVEGAEEQGGELKGLCPSDEEMGRKAVNYVFKTPGGNIYHGADSHYSIQ
FAKHGKEFDIDVALNNYGENPVGIADKMTSIDLLRMAECLRTNVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYKFHP
FIWEVGGKYVYPRDKDLIEYHHPRGFDDCFEQEPNIQFKSML

Specific function: Probably catalyzes the hydrolysis of L-ascorbate-6-P into 3-keto-L-gulonate-6-P. Is essential for L-ascorbate utilization under anaerobic conditions [H]

COG id: COG2220

COG function: function code R; Predicted Zn-dependent hydrolases of the beta-lactamase fold

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ulaG family [H]

Homologues:

Organism=Escherichia coli, GI87082387, Length=363, Percent_Identity=62.2589531680441, Blast_Score=478, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 41589; Mature: 41458

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVNEITRESWILSTFPEWGTWLNEEIEQEVVPEGNFAMWWLGCVGVWIKTPAGANICM
CCCHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHEEECCCCCHHHH
DLWCSRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVLDPFAINEVDFILASHYHS
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHCCCC
DHIDVNVAAAIVNNPNLNHVKFVGPWHCGELWKKWGVPEDRIIVVKPGDVVKIKDVEIHA
CCCEEEEEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCCCEEEECCCCEEEEEEEEEEE
LDSFDRTCLVTLPVEGAEEQGGELKGLCPSDEEMGRKAVNYVFKTPGGNIYHGADSHYSI
ECCCCCEEEEEEECCCCHHCCCCEECCCCCHHHHHHHHHHEEEECCCCCEEECCCCCEEE
QFAKHGKEFDIDVALNNYGENPVGIADKMTSIDLLRMAECLRTNVIIPVHHDIWTNFMAS
EEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHH
TQEIIDLWRMRKDRLQYKFHPFIWEVGGKYVYPRDKDLIEYHHPRGFDDCFEQEPNIQFK
HHHHHHHHHHHHHHHCEEECCEEEECCCEEECCCCCCHHHHCCCCCCHHHHCCCCCEEEE
SML
CCC
>Mature Secondary Structure 
AKVNEITRESWILSTFPEWGTWLNEEIEQEVVPEGNFAMWWLGCVGVWIKTPAGANICM
CCHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHEEECCCCCHHHH
DLWCSRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVLDPFAINEVDFILASHYHS
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHCCCC
DHIDVNVAAAIVNNPNLNHVKFVGPWHCGELWKKWGVPEDRIIVVKPGDVVKIKDVEIHA
CCCEEEEEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCCCEEEECCCCEEEEEEEEEEE
LDSFDRTCLVTLPVEGAEEQGGELKGLCPSDEEMGRKAVNYVFKTPGGNIYHGADSHYSI
ECCCCCEEEEEEECCCCHHCCCCEECCCCCHHHHHHHHHHEEEECCCCCEEECCCCCEEE
QFAKHGKEFDIDVALNNYGENPVGIADKMTSIDLLRMAECLRTNVIIPVHHDIWTNFMAS
EEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHH
TQEIIDLWRMRKDRLQYKFHPFIWEVGGKYVYPRDKDLIEYHHPRGFDDCFEQEPNIQFK
HHHHHHHHHHHHHHHCEEECCEEEECCCEEECCCCCCHHHHCCCCCCHHHHCCCCCEEEE
SML
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA