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The map label for this gene is manZ [H]

Identifier: 126209124

GI number: 126209124

Start: 1886502

End: 1887329

Strand: Reverse

Name: manZ [H]

Synonym: APL_1660

Alternate gene names: 126209124

Gene position: 1887329-1886502 (Counterclockwise)

Preceding gene: 126209125

Following gene: 126209123

Centisome position: 82.98

GC content: 45.53

Gene sequence:

>828_bases
ATGTCAGAGAAAAAACAACTTACCAGTGCGGATATTCGTGCGACTTATTGGCGTTCAACTTTCCTATTAGGATCATTTAA
CTTTGAACGTATGCAATCAATGGGCTTCTGCGTATCGATGATCCCAACGATTAAACGTCTTTACAGCCGTAAAGAAGATC
AAGCGGCGGCATTAAAACGCCACTTAGAGTTCTTTAATACCCAGCCGTGGGTCGGTTCGGCGATTATGGGTGTAACCGCT
GCGATGGAACAAGAACGTGCTAACGGAGCAGACATTGACGATGCGGCAATTAGCGGGGTGAAAGTCGGTTTAATGGGGCC
GCTTGCCGGTGTCGGCGATCCGATTTTCTGGGGAACATTACGTCCGGTATTAGCCGCACTAGGTGCAGGTTTAGCGATTA
GCGGTAGCATTTTAGGTCCACTATTATTCTTTATCGGTATTAACCTTTGCCGTGCGCTTACCCGTTGGTACGGCTTCAAA
TACGGCTACCAAAAAGGAACGGAAATCGTTTCGGATATGGGTGGCGGTCGCCTGCAAAAAGTCACGCAAGGCGCTTCGAT
TCTCGGCTTATTTGTAATGGGTTCGTTGGTATCGAAGTGGACCAGTATCAACATACCGTTTGAGCTTTCTCGCTATAAAA
ATGCAATGGGTGAAGAAGTGGTTACCACCGTGCAAAGCGTTCTTAATGACTTATTACCGGGTTTAGCGGCATTATTACTG
ACCTTCTTATGTATGTGGTTATTACGTAAGAAAGTAAATGCGATGTACATTATCTTCGCGCTATTCGGCGTCGGTATTTT
AGGTTATTGGTTAGGCATTTTAGCTTAA

Upstream 100 bases:

>100_bases
TACATCCAATTAAACCCACAATTCAACCAATCGGTAAGTGCGCCTCGTACTACGAAAAAATTAGCGGATAACGAGTTAGA
AGGTCTATAAGGAGCAAATT

Downstream 100 bases:

>100_bases
TTCCCTCTTTAACTCGTTTGCCCCCTCTTTATCCAAGAGGGGGTATTTTTTATAATACAAGCGGTCAAATTTACAGGAAA
TTTTGCAATGGATTTAAAAA

Product: mannose permease IID component

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSEKKQLTSADIRATYWRSTFLLGSFNFERMQSMGFCVSMIPTIKRLYSRKEDQAAALKRHLEFFNTQPWVGSAIMGVTA
AMEQERANGADIDDAAISGVKVGLMGPLAGVGDPIFWGTLRPVLAALGAGLAISGSILGPLLFFIGINLCRALTRWYGFK
YGYQKGTEIVSDMGGGRLQKVTQGASILGLFVMGSLVSKWTSINIPFELSRYKNAMGEEVVTTVQSVLNDLLPGLAALLL
TFLCMWLLRKKVNAMYIIFALFGVGILGYWLGILA

Sequences:

>Translated_275_residues
MSEKKQLTSADIRATYWRSTFLLGSFNFERMQSMGFCVSMIPTIKRLYSRKEDQAAALKRHLEFFNTQPWVGSAIMGVTA
AMEQERANGADIDDAAISGVKVGLMGPLAGVGDPIFWGTLRPVLAALGAGLAISGSILGPLLFFIGINLCRALTRWYGFK
YGYQKGTEIVSDMGGGRLQKVTQGASILGLFVMGSLVSKWTSINIPFELSRYKNAMGEEVVTTVQSVLNDLLPGLAALLL
TFLCMWLLRKKVNAMYIIFALFGVGILGYWLGILA
>Mature_274_residues
SEKKQLTSADIRATYWRSTFLLGSFNFERMQSMGFCVSMIPTIKRLYSRKEDQAAALKRHLEFFNTQPWVGSAIMGVTAA
MEQERANGADIDDAAISGVKVGLMGPLAGVGDPIFWGTLRPVLAALGAGLAISGSILGPLLFFIGINLCRALTRWYGFKY
GYQKGTEIVSDMGGGRLQKVTQGASILGLFVMGSLVSKWTSINIPFELSRYKNAMGEEVVTTVQSVLNDLLPGLAALLLT
FLCMWLLRKKVNAMYIIFALFGVGILGYWLGILA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=274, Percent_Identity=64.2335766423358, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1789529, Length=273, Percent_Identity=32.967032967033, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 30045; Mature: 29914

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKKQLTSADIRATYWRSTFLLGSFNFERMQSMGFCVSMIPTIKRLYSRKEDQAAALKR
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
HLEFFNTQPWVGSAIMGVTAAMEQERANGADIDDAAISGVKVGLMGPLAGVGDPIFWGTL
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHCCCCCCCCCHHHHHH
RPVLAALGAGLAISGSILGPLLFFIGINLCRALTRWYGFKYGYQKGTEIVSDMGGGRLQK
HHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCHHHHHHHHHHCCCCHHHH
VTQGASILGLFVMGSLVSKWTSINIPFELSRYKNAMGEEVVTTVQSVLNDLLPGLAALLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFLCMWLLRKKVNAMYIIFALFGVGILGYWLGILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SEKKQLTSADIRATYWRSTFLLGSFNFERMQSMGFCVSMIPTIKRLYSRKEDQAAALKR
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
HLEFFNTQPWVGSAIMGVTAAMEQERANGADIDDAAISGVKVGLMGPLAGVGDPIFWGTL
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHCCCCCCCCCHHHHHH
RPVLAALGAGLAISGSILGPLLFFIGINLCRALTRWYGFKYGYQKGTEIVSDMGGGRLQK
HHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCHHHHHHHHHHCCCCHHHH
VTQGASILGLFVMGSLVSKWTSINIPFELSRYKNAMGEEVVTTVQSVLNDLLPGLAALLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFLCMWLLRKKVNAMYIIFALFGVGILGYWLGILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]