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The map label for this gene is lipA

Identifier: 126209057

GI number: 126209057

Start: 1820527

End: 1821519

Strand: Reverse

Name: lipA

Synonym: APL_1593

Alternate gene names: 126209057

Gene position: 1821519-1820527 (Counterclockwise)

Preceding gene: 126209058

Following gene: 126209052

Centisome position: 80.09

GC content: 46.42

Gene sequence:

>993_bases
ATGACAACTGCGACAGGTACTAAGCCTAAAAAAATGGAAGCGTTTAAAATGGAGCGTGGCGTGAAATATCGCGATGCTGC
CAAAACATCGGTAATTCAAGTACGTAATATCGACCCCGATCAAGAGCTATTACCGAAACCGAGTTGGATGAAAATTAAAT
TACCGGCGGCTTCGGCGAAAATCGACAGTATCAAACACGGTATGCGCCGTCACGGCTTACACTCCGTTTGTGAAGAAGCG
TCTTGTCCTAATTTACACGAATGTTTTAACCACGGTACGGCAACCTTTATGATTATGGGTGCAATCTGTACGCGTCGTTG
TCCGTTCTGTGACGTTGCACACGGTAAACCGCTACCGCTAGATCCGGAAGAACCGCGTAAAGTGGCGGAAACCGTGCAAG
ATATGAAACTAAAATATGTGGTAATCACTTCGGTGGATCGTGACGATTTAGCTGACCGCGGAGCGGCGCACTTTGCGGCG
ACAGTACGTGAAATCAAAGCCTTGAATCCGGAATGTAAAGTGGAGATTCTAGTGCCGGATTTCCGTGGCCGTGTAGAACA
AGCGGTCGAAATTTTAAAACAAAATCCGCCGGACGTATTCAACCACAATCTTGAGAATGTGCCGCGTTTATACCGTGAAG
TACGCCCGGGTGCGGACTACAAATGGTCGCTTGAATTACTTAAAATCTTCAAACAAGAATTTCCGAATATTCCGACCAAA
TCGGGTTTAATGGTAGGGCTTGGCGAAACCAACGAAGAAATTTTAGAAGTGATGCAAGACTTGCGCGACCACGGCGTAAC
CATGCTGACGATCGGTCAATATTTACAACCTAGCCGTCACCATTTAAAAGTGGAACGTTATGTACCGCCGGAAGAATTTG
ATATGTTCCGTAGCGAAGCGGAAAGAATGGGCTTTGAGCATGCCGCTTGCGGTCCGTTTGTACGTTCTTCCTATCACGCC
GATTTACAAGCGAAAGGCGAATTAGTCAAATAA

Upstream 100 bases:

>100_bases
TATTTGCCCGTTAAGAGCAAATCATCAGAATTATAATATCTGTGTGTCGGGGCGGAGTTATCTCCGCCCTTTAACATTGC
ATAGAGAAGGATAGAACTTA

Downstream 100 bases:

>100_bases
ATTGCAAAAAGAAAGAAAAAGCCGACCGCTTTCTCTTTTCGTATAAGTTGATAGCAAAATGGCGAGCATAAGCTCGCCAT
TTATTTTCCTTAGGTAAGGC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTTATGTKPKKMEAFKMERGVKYRDAAKTSVIQVRNIDPDQELLPKPSWMKIKLPAASAKIDSIKHGMRRHGLHSVCEEA
SCPNLHECFNHGTATFMIMGAICTRRCPFCDVAHGKPLPLDPEEPRKVAETVQDMKLKYVVITSVDRDDLADRGAAHFAA
TVREIKALNPECKVEILVPDFRGRVEQAVEILKQNPPDVFNHNLENVPRLYREVRPGADYKWSLELLKIFKQEFPNIPTK
SGLMVGLGETNEEILEVMQDLRDHGVTMLTIGQYLQPSRHHLKVERYVPPEEFDMFRSEAERMGFEHAACGPFVRSSYHA
DLQAKGELVK

Sequences:

>Translated_330_residues
MTTATGTKPKKMEAFKMERGVKYRDAAKTSVIQVRNIDPDQELLPKPSWMKIKLPAASAKIDSIKHGMRRHGLHSVCEEA
SCPNLHECFNHGTATFMIMGAICTRRCPFCDVAHGKPLPLDPEEPRKVAETVQDMKLKYVVITSVDRDDLADRGAAHFAA
TVREIKALNPECKVEILVPDFRGRVEQAVEILKQNPPDVFNHNLENVPRLYREVRPGADYKWSLELLKIFKQEFPNIPTK
SGLMVGLGETNEEILEVMQDLRDHGVTMLTIGQYLQPSRHHLKVERYVPPEEFDMFRSEAERMGFEHAACGPFVRSSYHA
DLQAKGELVK
>Mature_329_residues
TTATGTKPKKMEAFKMERGVKYRDAAKTSVIQVRNIDPDQELLPKPSWMKIKLPAASAKIDSIKHGMRRHGLHSVCEEAS
CPNLHECFNHGTATFMIMGAICTRRCPFCDVAHGKPLPLDPEEPRKVAETVQDMKLKYVVITSVDRDDLADRGAAHFAAT
VREIKALNPECKVEILVPDFRGRVEQAVEILKQNPPDVFNHNLENVPRLYREVRPGADYKWSLELLKIFKQEFPNIPTKS
GLMVGLGETNEEILEVMQDLRDHGVTMLTIGQYLQPSRHHLKVERYVPPEEFDMFRSEAERMGFEHAACGPFVRSSYHAD
LQAKGELVK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=286, Percent_Identity=45.1048951048951, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=45.2, Blast_Score=213, Evalue=1e-55,
Organism=Escherichia coli, GI1786846, Length=315, Percent_Identity=71.7460317460317, Blast_Score=480, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI32564533, Length=283, Percent_Identity=42.756183745583, Blast_Score=224, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=281, Percent_Identity=44.4839857651246, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI221513272, Length=295, Percent_Identity=46.1016949152542, Blast_Score=248, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_ACTP2 (A3N2P1)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054282.1
- ProteinModelPortal:   A3N2P1
- STRING:   A3N2P1
- GeneID:   4849701
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1593
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   A3N2P1
- ProtClustDB:   PRK05481
- BioCyc:   APLE416269:APL_1593-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 37443; Mature: 37311

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTATGTKPKKMEAFKMERGVKYRDAAKTSVIQVRNIDPDQELLPKPSWMKIKLPAASAK
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHEEEEECCCCCHHHCCCCCCEEEECCCCHHH
IDSIKHGMRRHGLHSVCEEASCPNLHECFNHGTATFMIMGAICTRRCPFCDVAHGKPLPL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
DPEEPRKVAETVQDMKLKYVVITSVDRDDLADRGAAHFAATVREIKALNPECKVEILVPD
CCCCHHHHHHHHHHCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
FRGRVEQAVEILKQNPPDVFNHNLENVPRLYREVRPGADYKWSLELLKIFKQEFPNIPTK
HHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCC
SGLMVGLGETNEEILEVMQDLRDHGVTMLTIGQYLQPSRHHLKVERYVPPEEFDMFRSEA
CCEEEECCCCCHHHHHHHHHHHHCCEEEEEHHHHHCCCHHHEEEECCCCHHHHHHHHHHH
ERMGFEHAACGPFVRSSYHADLQAKGELVK
HHCCCCHHHCCHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTATGTKPKKMEAFKMERGVKYRDAAKTSVIQVRNIDPDQELLPKPSWMKIKLPAASAK
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHEEEEECCCCCHHHCCCCCCEEEECCCCHHH
IDSIKHGMRRHGLHSVCEEASCPNLHECFNHGTATFMIMGAICTRRCPFCDVAHGKPLPL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
DPEEPRKVAETVQDMKLKYVVITSVDRDDLADRGAAHFAATVREIKALNPECKVEILVPD
CCCCHHHHHHHHHHCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
FRGRVEQAVEILKQNPPDVFNHNLENVPRLYREVRPGADYKWSLELLKIFKQEFPNIPTK
HHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCC
SGLMVGLGETNEEILEVMQDLRDHGVTMLTIGQYLQPSRHHLKVERYVPPEEFDMFRSEA
CCEEEECCCCCHHHHHHHHHHHHCCEEEEEHHHHHCCCHHHEEEECCCCHHHHHHHHHHH
ERMGFEHAACGPFVRSSYHADLQAKGELVK
HHCCCCHHHCCHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA