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The map label for this gene is mscL

Identifier: 126209056

GI number: 126209056

Start: 1820008

End: 1820397

Strand: Direct

Name: mscL

Synonym: APL_1592

Alternate gene names: 126209056

Gene position: 1820008-1820397 (Clockwise)

Preceding gene: 126209055

Following gene: 126209070

Centisome position: 80.02

GC content: 41.03

Gene sequence:

>390_bases
ATGAGCATTTTAAAAGAATTCCGTGAATTTGCGGTAAAAGGTAACGTAGTCGATATGGCTGTCGGTGTGATCATCGGCGG
TGCATTCGGTAAAATCGTCTCTTCATTAGTCAGCGATGTAGTAATGCCGCCTATCGGTTGGTTAATCGGCGGCGTAGATT
TTAAAGATCTCGCCATCGAAATCGCACCGGCAAAAGAAGGTGCCGAAGCGGTAATGTTAAAATACGGTGCGTTTATTCAA
AACGTATTTGATTTCTTAATTATCGCTATCGCCGTATTCGGTATGGTAAAAGTAATCAACAAAATTAAAAAACCGGCGGA
AGCCGCACCGGCTGAACCGACAGCGGAAGAAAAATTATTAACTGAAATTCGTGATTTATTGAAAAAATAA

Upstream 100 bases:

>100_bases
AAATTTAGTTAAAAGTGTTAATAGGATATTACCGTTTGTTTTATAATTAACCGATTAATGCCTAATAGTGGGTATAAATT
TCAATGAAAAAGGAAAAGCA

Downstream 100 bases:

>100_bases
TTCGAATAAAAAAGAGGGAGCGGAAGCTCGCCTTACCTAAGGAAAATAAATGGCGAGCTTATGCTCGCCATTTTGCTATC
AACTTATACGAAAAGAGAAA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPPIGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQ
NVFDFLIIAIAVFGMVKVINKIKKPAEAAPAEPTAEEKLLTEIRDLLKK

Sequences:

>Translated_129_residues
MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPPIGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQ
NVFDFLIIAIAVFGMVKVINKIKKPAEAAPAEPTAEEKLLTEIRDLLKK
>Mature_128_residues
SILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPPIGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQN
VFDFLIIAIAVFGMVKVINKIKKPAEAAPAEPTAEEKLLTEIRDLLKK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=132, Percent_Identity=65.1515151515152, Blast_Score=167, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_ACTP2 (A3N2P0)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054281.1
- ProteinModelPortal:   A3N2P0
- SMR:   A3N2P0
- STRING:   A3N2P0
- GeneID:   4849008
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1592
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- PhylomeDB:   A3N2P0
- ProtClustDB:   PRK00567
- BioCyc:   APLE416269:APL_1592-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 13846; Mature: 13715

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e72de58)-; HASH(0x1ec693dc)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPPIGWLIGGVDFKDLAIE
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHEE
IAPAKEGAEAVMLKYGAFIQNVFDFLIIAIAVFGMVKVINKIKKPAEAAPAEPTAEEKLL
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
TEIRDLLKK
HHHHHHHCH
>Mature Secondary Structure 
SILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPPIGWLIGGVDFKDLAIE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHEE
IAPAKEGAEAVMLKYGAFIQNVFDFLIIAIAVFGMVKVINKIKKPAEAAPAEPTAEEKLL
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
TEIRDLLKK
HHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA