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The map label for this gene is ispH

Identifier: 126208984

GI number: 126208984

Start: 1734606

End: 1735550

Strand: Direct

Name: ispH

Synonym: APL_1520

Alternate gene names: 126208984

Gene position: 1734606-1735550 (Clockwise)

Preceding gene: 126208983

Following gene: 126208985

Centisome position: 76.26

GC content: 46.35

Gene sequence:

>945_bases
ATGAATATTTTATTAGCAAACCCAAGAGGGTTCTGTGCCGGCGTTGATCGAGCGATTTCGATTGTGGAACTTGCCCTTGA
AATTCACGGCGCACCGATTTATGTACGTCACGAAGTGGTACATAACCGCTTTGTGGTGGACGGTTTAAAAGCCAAAGGTG
CGGTGTTTGTCGAAGAGTTGGACGAAGTGCCTGACGGTGCGATTGTGATTTTCTCGGCACACGGTGTCTCGCAAGAGGTT
CGCCAAGAAGCTAAACGCCGCGGCTTAAAAGTATTTGACGCAACTTGCCCGTTAGTAACAAAAGTACATATGCAAGTGGC
TCGTGCCAGCAAAAAAGGCACTAAAGCGATTTTAATCGGACATGAAGGTCATCCGGAAGTGATCGGCACGATGGGGCAAT
ACGATAACCAAGACGCCGGTATATTCTTGGTGGAATCGGTCGAAGATATTGCCAAACTGCCGGTCAGCAGCCAAGACGAT
TTAACTTTTATGACGCAAACCACACTTTCGATTGACGACACCAGCGATGTGATTGAAGCGTTAAAAGAGAAATATCCGGC
AATTCAAGGCCCGCGCAAAAACGATATTTGTTATGCGACAACCAACCGCCAACAAGCGGTACGAGAATTGGCAAAACAGT
CTCAGCTTGTATTAGTGGTCGGTTCCAAAAATTCGTCAAATTCTAACCGCTTGGCAGAACTGGCTTCACGTATGGGTGTA
CCGTCTAAATTGATTGACGGTCCTCAAGATATTGATCCGAGTTGGTTGGACGGTGTGGAAACTATCGGCATTACCGCCGG
TGCATCGGCACCGGAAGTGTTAGTACAATCGGTGGTTGAACACTTAAAAACTTTAGGTGTAACCGGTGTGAGCAACTTAG
AAGGTTGCGAAGAAAATATGGTATTTGAAGTGCCGAAAGAACTACGTATTCATGAGGTGAAATAA

Upstream 100 bases:

>100_bases
TCATTATCCCGTATTTAATGTGGCGGATATTGCCATTGTAGTGGGTGCGGGTTTGCTCATCTTGGAAGCATTCCTCGACA
AAAAGAAAAAGAGTGATTAA

Downstream 100 bases:

>100_bases
TGGCTGAATTAAATCGTTTTAGAATTGAGTGGGAATGCCGCCGTGGTATGCGCGAATTAGACAAAATGATTATGCCTTTT
TACAAAGCGCATTTTGATGA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MNILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNRFVVDGLKAKGAVFVEELDEVPDGAIVIFSAHGVSQEV
RQEAKRRGLKVFDATCPLVTKVHMQVARASKKGTKAILIGHEGHPEVIGTMGQYDNQDAGIFLVESVEDIAKLPVSSQDD
LTFMTQTTLSIDDTSDVIEALKEKYPAIQGPRKNDICYATTNRQQAVRELAKQSQLVLVVGSKNSSNSNRLAELASRMGV
PSKLIDGPQDIDPSWLDGVETIGITAGASAPEVLVQSVVEHLKTLGVTGVSNLEGCEENMVFEVPKELRIHEVK

Sequences:

>Translated_314_residues
MNILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNRFVVDGLKAKGAVFVEELDEVPDGAIVIFSAHGVSQEV
RQEAKRRGLKVFDATCPLVTKVHMQVARASKKGTKAILIGHEGHPEVIGTMGQYDNQDAGIFLVESVEDIAKLPVSSQDD
LTFMTQTTLSIDDTSDVIEALKEKYPAIQGPRKNDICYATTNRQQAVRELAKQSQLVLVVGSKNSSNSNRLAELASRMGV
PSKLIDGPQDIDPSWLDGVETIGITAGASAPEVLVQSVVEHLKTLGVTGVSNLEGCEENMVFEVPKELRIHEVK
>Mature_314_residues
MNILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNRFVVDGLKAKGAVFVEELDEVPDGAIVIFSAHGVSQEV
RQEAKRRGLKVFDATCPLVTKVHMQVARASKKGTKAILIGHEGHPEVIGTMGQYDNQDAGIFLVESVEDIAKLPVSSQDD
LTFMTQTTLSIDDTSDVIEALKEKYPAIQGPRKNDICYATTNRQQAVRELAKQSQLVLVVGSKNSSNSNRLAELASRMGV
PSKLIDGPQDIDPSWLDGVETIGITAGASAPEVLVQSVVEHLKTLGVTGVSNLEGCEENMVFEVPKELRIHEVK

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=69.6774193548387, Blast_Score=451, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_ACTP2 (A3N2G8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054209.1
- STRING:   A3N2G8
- GeneID:   4849344
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1520
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- PhylomeDB:   A3N2G8
- ProtClustDB:   PRK01045
- BioCyc:   APLE416269:APL_1520-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34035; Mature: 34035

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNRFVVDGLKAKGAVFVEEL
CCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHEEECCCCCCCHHHHHH
DEVPDGAIVIFSAHGVSQEVRQEAKRRGLKVFDATCPLVTKVHMQVARASKKGTKAILIG
HHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEE
HEGHPEVIGTMGQYDNQDAGIFLVESVEDIAKLPVSSQDDLTFMTQTTLSIDDTSDVIEA
CCCCCHHHCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCEEEEEEEEEECCCHHHHHHH
LKEKYPAIQGPRKNDICYATTNRQQAVRELAKQSQLVLVVGSKNSSNSNRLAELASRMGV
HHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCC
PSKLIDGPQDIDPSWLDGVETIGITAGASAPEVLVQSVVEHLKTLGVTGVSNLEGCEENM
CHHHCCCCCCCCCHHHCCHHHEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
VFEVPKELRIHEVK
EEECCCCCCEECCC
>Mature Secondary Structure
MNILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNRFVVDGLKAKGAVFVEEL
CCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHEEECCCCCCCHHHHHH
DEVPDGAIVIFSAHGVSQEVRQEAKRRGLKVFDATCPLVTKVHMQVARASKKGTKAILIG
HHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEE
HEGHPEVIGTMGQYDNQDAGIFLVESVEDIAKLPVSSQDDLTFMTQTTLSIDDTSDVIEA
CCCCCHHHCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCEEEEEEEEEECCCHHHHHHH
LKEKYPAIQGPRKNDICYATTNRQQAVRELAKQSQLVLVVGSKNSSNSNRLAELASRMGV
HHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCC
PSKLIDGPQDIDPSWLDGVETIGITAGASAPEVLVQSVVEHLKTLGVTGVSNLEGCEENM
CHHHCCCCCCCCCHHHCCHHHEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
VFEVPKELRIHEVK
EEECCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA