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The map label for this gene is trmE

Identifier: 126208954

GI number: 126208954

Start: 1705081

End: 1706439

Strand: Direct

Name: trmE

Synonym: APL_1490

Alternate gene names: 126208954

Gene position: 1705081-1706439 (Clockwise)

Preceding gene: 126208953

Following gene: 126208958

Centisome position: 74.97

GC content: 47.68

Gene sequence:

>1359_bases
ATGAAAGATACGATTGTCGCTCAGGCAACCCCGATTGGTCGTGGGGGCGTGGGCATTTTACGCATCTCGGGGCCATTGGC
TCAAGAGGTGGCAAAAGAAGTGCTGGGCAAAGAGCTAAAACCTCGTCTTGCCAATTACTTACCGTTTAAAGATCAAGACG
GCACGGTGTTAGACCAAGGTATTGCGTTGTTCTTTAAAGCGCCGAACTCGTTTACCGGAGAAGACGTGTTAGAACTGCAA
GGGCATGGCGGTCAAGTCATCTTAGATATTTTACTGAAACGTATTTTAACAATTAAAGGCATACGTATTGCCCGTGCCGG
CGAGTTTTCCGAGCAAGCCTTTTTAAACGATAAACTCGACCTCGCACAAGCAGAAGCGATTGCCGATTTAATTGATGCGA
CCAGCGAGCAAGCGGCACGTTCGGCGTTAAAATCGCTACAAGGCGAGTTCTCTAATAAGATTAATCAGCTAGTCGATTCG
GTCATTTATCTGCGCACCTATGTGGAAGCGGCGATTGATTTCCCTGATGAAGAAATCGATTTTTTAGCGGACGGCAAAAT
CGAAGGGCATTTAAACGATACTATTCGTCAACTGAACGGCGTACGCAAAGAAGCGAAACAAGGTGCGATTTTGCGTGAAG
GGATGAAAGTGGTGATTGCCGGTCGCCCGAATGCCGGTAAATCGAGCCTGTTAAACGCTTTAGCGGGCAGAGAAGCGGCG
ATTGTAACCAATATTGCCGGTACTACCCGTGACGTATTGCGTGAGCATATTCACATTGACGGTATGCCGCTGCATATTAT
TGATACCGCCGGTTTACGTGAAGCGAGTGATGAGGTGGAGAAAATCGGGATCCAACGTGCGTGGGATGAGATCGAGCAGG
CGGATCACGTATTATTGATGATCGACAGCACCGAACAAACGGCGGAAGCATTTAAGACCGAATGGGCGGACTTTTTAGCC
AAATTACCGCAAAGCATTCCGGTTACCGTGATTCGTAACAAAGTGGATTTATCCGGCGAGGCTGAAGGCTTACAAGAGTT
GGACGGTTTTACTTTGATTCGTTTATCGGCACAAACCAAAGTGGGCGTGGACTTACTGCGTGAGCACCTTAAAAAATCAA
TGGGTTATCAAAGCTCGACCGAGGGCGGCTTCTTAGCTCGCCGCCGTCATTTACAGGCATTGGAAACCGCCGCAGAGCAT
TTAGAACGCGGCCATATCCAATTAACCCAATTCTTCGCCGGCGAATTACTCGCAGAAGAATTGCGTATGGTACAAAATGC
GTTAAGCGAAATTACCGGTCAATTTACCTCGGATGATCTGCTAGGCAATATTTTCAGCTCGTTCTGTATCGGTAAATAA

Upstream 100 bases:

>100_bases
TAGCACCTTCCGTTTTATGCTATAATAGAACGACTACAAGCGGTCCGATTTGCAAATTTTTTTGCAAAAACGACCGCTTC
TTTAATAAGAAGGGATCCAA

Downstream 100 bases:

>100_bases
GCCTAAGAAAGTGAAGATATAAAAAACAAGCGGTCAATTTCTCAGGATTTTTGCAAAATTCCTATTGAAATTGACCGCTT
TTTATAAGTTTTAAAGCTAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKDTIVAQATPIGRGGVGILRISGPLAQEVAKEVLGKELKPRLANYLPFKDQDGTVLDQGIALFFKAPNSFTGEDVLELQ
GHGGQVILDILLKRILTIKGIRIARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINQLVDS
VIYLRTYVEAAIDFPDEEIDFLADGKIEGHLNDTIRQLNGVRKEAKQGAILREGMKVVIAGRPNAGKSSLLNALAGREAA
IVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVEKIGIQRAWDEIEQADHVLLMIDSTEQTAEAFKTEWADFLA
KLPQSIPVTVIRNKVDLSGEAEGLQELDGFTLIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQALETAAEH
LERGHIQLTQFFAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCIGK

Sequences:

>Translated_452_residues
MKDTIVAQATPIGRGGVGILRISGPLAQEVAKEVLGKELKPRLANYLPFKDQDGTVLDQGIALFFKAPNSFTGEDVLELQ
GHGGQVILDILLKRILTIKGIRIARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINQLVDS
VIYLRTYVEAAIDFPDEEIDFLADGKIEGHLNDTIRQLNGVRKEAKQGAILREGMKVVIAGRPNAGKSSLLNALAGREAA
IVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVEKIGIQRAWDEIEQADHVLLMIDSTEQTAEAFKTEWADFLA
KLPQSIPVTVIRNKVDLSGEAEGLQELDGFTLIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQALETAAEH
LERGHIQLTQFFAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCIGK
>Mature_452_residues
MKDTIVAQATPIGRGGVGILRISGPLAQEVAKEVLGKELKPRLANYLPFKDQDGTVLDQGIALFFKAPNSFTGEDVLELQ
GHGGQVILDILLKRILTIKGIRIARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINQLVDS
VIYLRTYVEAAIDFPDEEIDFLADGKIEGHLNDTIRQLNGVRKEAKQGAILREGMKVVIAGRPNAGKSSLLNALAGREAA
IVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVEKIGIQRAWDEIEQADHVLLMIDSTEQTAEAFKTEWADFLA
KLPQSIPVTVIRNKVDLSGEAEGLQELDGFTLIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQALETAAEH
LERGHIQLTQFFAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=466, Percent_Identity=34.549356223176, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI193082988, Length=497, Percent_Identity=32.5955734406439, Blast_Score=222, Evalue=7e-58,
Organism=Homo sapiens, GI193082993, Length=462, Percent_Identity=33.7662337662338, Blast_Score=211, Evalue=1e-54,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=75.609756097561, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=94, Percent_Identity=41.4893617021277, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=460, Percent_Identity=26.9565217391304, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=31.3883299798793, Blast_Score=209, Evalue=6e-55,
Organism=Drosophila melanogaster, GI45550813, Length=483, Percent_Identity=31.4699792960663, Blast_Score=198, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_ACTP2 (A3N2D8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054179.1
- ProteinModelPortal:   A3N2D8
- SMR:   A3N2D8
- STRING:   A3N2D8
- GeneID:   4850018
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1490
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   A3N2D8
- ProtClustDB:   PRK05291
- BioCyc:   APLE416269:APL_1490-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49448; Mature: 49448

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: BINDING 21-21 BINDING 78-78 BINDING 118-118 BINDING 452-452

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTIVAQATPIGRGGVGILRISGPLAQEVAKEVLGKELKPRLANYLPFKDQDGTVLDQG
CCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCC
IALFFKAPNSFTGEDVLELQGHGGQVILDILLKRILTIKGIRIARAGEFSEQAFLNDKLD
EEEEEECCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCHH
LAQAEAIADLIDATSEQAARSALKSLQGEFSNKINQLVDSVIYLRTYVEAAIDFPDEEID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHC
FLADGKIEGHLNDTIRQLNGVRKEAKQGAILREGMKVVIAGRPNAGKSSLLNALAGREAA
EEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCE
IVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVEKIGIQRAWDEIEQADHVLLM
EEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
IDSTEQTAEAFKTEWADFLAKLPQSIPVTVIRNKVDLSGEAEGLQELDGFTLIRLSAQTK
EECCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHCCCEEEEEEECCHH
VGVDLLREHLKKSMGYQSSTEGGFLARRRHLQALETAAEHLERGHIQLTQFFAGELLAEE
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LRMVQNALSEITGQFTSDDLLGNIFSSFCIGK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDTIVAQATPIGRGGVGILRISGPLAQEVAKEVLGKELKPRLANYLPFKDQDGTVLDQG
CCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCC
IALFFKAPNSFTGEDVLELQGHGGQVILDILLKRILTIKGIRIARAGEFSEQAFLNDKLD
EEEEEECCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCHH
LAQAEAIADLIDATSEQAARSALKSLQGEFSNKINQLVDSVIYLRTYVEAAIDFPDEEID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHC
FLADGKIEGHLNDTIRQLNGVRKEAKQGAILREGMKVVIAGRPNAGKSSLLNALAGREAA
EEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCE
IVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVEKIGIQRAWDEIEQADHVLLM
EEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
IDSTEQTAEAFKTEWADFLAKLPQSIPVTVIRNKVDLSGEAEGLQELDGFTLIRLSAQTK
EECCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHCCCEEEEEEECCHH
VGVDLLREHLKKSMGYQSSTEGGFLARRRHLQALETAAEHLERGHIQLTQFFAGELLAEE
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LRMVQNALSEITGQFTSDDLLGNIFSSFCIGK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA