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The map label for this gene is tyrS [H]

Identifier: 126208940

GI number: 126208940

Start: 1690213

End: 1691403

Strand: Reverse

Name: tyrS [H]

Synonym: APL_1476

Alternate gene names: 126208940

Gene position: 1691403-1690213 (Counterclockwise)

Preceding gene: 126208941

Following gene: 126208926

Centisome position: 74.36

GC content: 43.07

Gene sequence:

>1191_bases
ATGGCTCAATCTATTGAAACGACATTAGCCGAATTGAAACGCGGTGTTGAAAATATTTATTCTGAAGAAGATTTAATTGA
AAAGTTAAAGGAAAATCGTCCGTTACGCATTAAATTAGGCGCAGACCCTACAGCACCCGATATTCATTTAGGTCATACCG
TTGTTTTAAATAAGCTCCGCCAATTCCAACAACTTGGTCATGAAGTGATTTTCTTAATCGGCGACTTTACCGGTATGGTC
GGTGATCCGTCAGGCAAAAACTCAACTCGCCCGCCGCTCACTCGTGAAGACGTGTTACGCAATGCGGAAACCTATAAACA
ACAATTATTCAAAATCTTAGACCCGAATAAAACGCGTGTGGTCTTCAACTCCGAATGGTTGGGTAAATTAGGGACGGAAG
GTATGATTCGCCTTGCCTCTAATTATACGGTTGCCCGTATGCTTGAGCGTGACGATTTCAAAAAACGTTTTACCAATAAT
CAATCTATCGCAATCCACGAATTTATTTATCCGTTACTTCAAGGTCATGATTCTGTTGCGTTAGAAGCGGATGTTGAGTT
AGGCGGTACAGACCAAACCTTTAACTTATTAGTCGGTCGTGAGTTACAAAAATCAGCAGGTCAAAAACCGCAGGTAGCAA
TGACATTACCATTACTTGTCGGCTTAGACGGCGAGAAAAAAATGTCGAAATCACTCGGTAACTATATCGGTGTAACCGAT
GCGCCGAGCGATATGTTCGGTAAAGTGATGTCGATTTCAGACGAGTTAATGTGGGATTGGTACAATCTACTTTCATTCCG
TCCGTTAGACGAAATCGCTCAATTGAAAGCGGACGTTGCGGAAGGTAAAAATCCGCGTGATGTGAAGATTCTTTTAGCGA
AAGAGTTAATTGCTCGTTTCCACGATCAAGCGGCGGCGGATTCGGCGGAACAAGAGTTTATCAACCGTTTCCAAAAAGGC
GCAATCCCGGATGAAATGCCGGAATTTACGTTTGAAGGCGAAATCGGTTTAGCGACTTTATTAAAAGAAGCCGGTTTAGT
CGCATCAACTTCCGAGGCGATTCGTTCCGCACAACAAGGCGGGGTAAAAATCGACGGCGAAAAAGTAGAAGACGTTCGTC
AAAATGCACAACAGGGTACGTTCGTTTACCAAGTCGGTAAACGTAAATTTGCCCGTGTAACGGTTAAATAA

Upstream 100 bases:

>100_bases
TTTTTTTTCGTCTATTGCCGATTAAACCTTATCAATTCCAATGTATCCCGTTATAATAGAAGTTATTTTCATATTTAAGA
CAAGATATAAGGAATCATAA

Downstream 100 bases:

>100_bases
GTCTTAGATATAAACGAAAAGGAAGCCTAGGCTTCCTTTTTTATGCTTTGGACAAGCGGTTAAATTTGTTAATTTTTTTG
CAAATTCGACCGCTTGTCAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAQSIETTLAELKRGVENIYSEEDLIEKLKENRPLRIKLGADPTAPDIHLGHTVVLNKLRQFQQLGHEVIFLIGDFTGMV
GDPSGKNSTRPPLTREDVLRNAETYKQQLFKILDPNKTRVVFNSEWLGKLGTEGMIRLASNYTVARMLERDDFKKRFTNN
QSIAIHEFIYPLLQGHDSVALEADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPLLVGLDGEKKMSKSLGNYIGVTD
APSDMFGKVMSISDELMWDWYNLLSFRPLDEIAQLKADVAEGKNPRDVKILLAKELIARFHDQAAADSAEQEFINRFQKG
AIPDEMPEFTFEGEIGLATLLKEAGLVASTSEAIRSAQQGGVKIDGEKVEDVRQNAQQGTFVYQVGKRKFARVTVK

Sequences:

>Translated_396_residues
MAQSIETTLAELKRGVENIYSEEDLIEKLKENRPLRIKLGADPTAPDIHLGHTVVLNKLRQFQQLGHEVIFLIGDFTGMV
GDPSGKNSTRPPLTREDVLRNAETYKQQLFKILDPNKTRVVFNSEWLGKLGTEGMIRLASNYTVARMLERDDFKKRFTNN
QSIAIHEFIYPLLQGHDSVALEADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPLLVGLDGEKKMSKSLGNYIGVTD
APSDMFGKVMSISDELMWDWYNLLSFRPLDEIAQLKADVAEGKNPRDVKILLAKELIARFHDQAAADSAEQEFINRFQKG
AIPDEMPEFTFEGEIGLATLLKEAGLVASTSEAIRSAQQGGVKIDGEKVEDVRQNAQQGTFVYQVGKRKFARVTVK
>Mature_395_residues
AQSIETTLAELKRGVENIYSEEDLIEKLKENRPLRIKLGADPTAPDIHLGHTVVLNKLRQFQQLGHEVIFLIGDFTGMVG
DPSGKNSTRPPLTREDVLRNAETYKQQLFKILDPNKTRVVFNSEWLGKLGTEGMIRLASNYTVARMLERDDFKKRFTNNQ
SIAIHEFIYPLLQGHDSVALEADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPLLVGLDGEKKMSKSLGNYIGVTDA
PSDMFGKVMSISDELMWDWYNLLSFRPLDEIAQLKADVAEGKNPRDVKILLAKELIARFHDQAAADSAEQEFINRFQKGA
IPDEMPEFTFEGEIGLATLLKEAGLVASTSEAIRSAQQGGVKIDGEKVEDVRQNAQQGTFVYQVGKRKFARVTVK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=411, Percent_Identity=26.0340632603406, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1787925, Length=410, Percent_Identity=28.2926829268293, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI25145802, Length=223, Percent_Identity=32.2869955156951, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6325160, Length=337, Percent_Identity=26.1127596439169, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI20130383, Length=403, Percent_Identity=26.0545905707196, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44222; Mature: 44091

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSIETTLAELKRGVENIYSEEDLIEKLKENRPLRIKLGADPTAPDIHLGHTVVLNKLR
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
QFQQLGHEVIFLIGDFTGMVGDPSGKNSTRPPLTREDVLRNAETYKQQLFKILDPNKTRV
HHHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
VFNSEWLGKLGTEGMIRLASNYTVARMLERDDFKKRFTNNQSIAIHEFIYPLLQGHDSVA
EECCHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCEE
LEADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPLLVGLDGEKKMSKSLGNYIGVTD
EEEECEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHEECCCHHHHHHHHCCCCCCCC
APSDMFGKVMSISDELMWDWYNLLSFRPLDEIAQLKADVAEGKNPRDVKILLAKELIARF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
HDQAAADSAEQEFINRFQKGAIPDEMPEFTFEGEIGLATLLKEAGLVASTSEAIRSAQQG
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHCCCCCCCHHHHHHHHHC
GVKIDGEKVEDVRQNAQQGTFVYQVGKRKFARVTVK
CEEECCHHHHHHHHHHHCCCEEEEECCCEEEEEECC
>Mature Secondary Structure 
AQSIETTLAELKRGVENIYSEEDLIEKLKENRPLRIKLGADPTAPDIHLGHTVVLNKLR
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
QFQQLGHEVIFLIGDFTGMVGDPSGKNSTRPPLTREDVLRNAETYKQQLFKILDPNKTRV
HHHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
VFNSEWLGKLGTEGMIRLASNYTVARMLERDDFKKRFTNNQSIAIHEFIYPLLQGHDSVA
EECCHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCEE
LEADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPLLVGLDGEKKMSKSLGNYIGVTD
EEEECEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHEECCCHHHHHHHHCCCCCCCC
APSDMFGKVMSISDELMWDWYNLLSFRPLDEIAQLKADVAEGKNPRDVKILLAKELIARF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
HDQAAADSAEQEFINRFQKGAIPDEMPEFTFEGEIGLATLLKEAGLVASTSEAIRSAQQG
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHCCCCCCCHHHHHHHHHC
GVKIDGEKVEDVRQNAQQGTFVYQVGKRKFARVTVK
CEEECCHHHHHHHHHHHCCCEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA