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The map label for this gene is rfbB1 [H]

Identifier: 126208936

GI number: 126208936

Start: 1684665

End: 1685738

Strand: Direct

Name: rfbB1 [H]

Synonym: APL_1472

Alternate gene names: 126208936

Gene position: 1684665-1685738 (Clockwise)

Preceding gene: 126208935

Following gene: 126208937

Centisome position: 74.07

GC content: 42.74

Gene sequence:

>1074_bases
ATGAAAACTATCTTTATTACAGGCGGTGCGGGTTTTATCGGATCTGCGCTCATACGTTATGTGATTAAGCATACCGATTG
TATTGCGGTCAATATCGATAAACTGACCTACGCAGGGAATTTGGCTTCTTTGGCAAGTGTGAGCAACAGTTCACGCTACT
ATTTTGAACAAGTCGATATTTGTGATAGCGGTCGAATTTCGCAGCTTTTTTGCAAATATCAACCGGATGCGGTTATGCAC
CTTGCGGCGGAAAGCCATGTAGATCGTTCGATTGACGGACCGGCGGCGTTTATGCAAACCAATATTATCGGTACTTATAC
GTTACTGGAAGCAAGCCGCCGATACTGGTTGGATTTGTCGCGAGAGAAAAAGCAAGATTTTCGTTTTCATCATATCTCGA
CCGATGAAGTGTACGGCGATTTAGGTCACGATAAAGGACTATTTACGGAAACTTCTCCTTACGCACCTTCCAGTCCTTAT
TCGGCCTCTAAAGCATCAAGTGATCATTTGGTTCGGGCTTGGTTTCGTACTTATGGTTTACCGACATTAGTGACAAATTG
CTCGAATAATTACGGGCCTTACCAGTTTCCGGAAAAATTAATTCCATTGATGATTTTAAATGCGCTTGAAGGAAAATCGT
TACCTGTGTATGGAAACGGTTTACAAATTCGAGATTGGCTATTTGTCGAAGATCACGTACGTGCGCTTTATAAAGTGTTA
TGTGACGGGAAGGTTGGAGAAACTTACAATATCGGCGGGCATAACGAAAAAACTAATATCGAAGTGGTGCGCGCCATTTG
CCGAATCTTAGATGAATTGGTGCCGAATAAACGTAATGGGATAAATAAGTACGAAGAGCTAATTACTTATGTAAAAGATC
GTCCGGGGCATGACGTTCGTTATGCAATTGATGCGAGTAAAATTTGCACTGATTTGGGCTGGCAGCCGCAAGAAACCTTT
GAATCGGGGCTACGGAAAACCGTCGAGTGGTATTTATCGAATAAAGATTGGTGGCAGTCGATACTTAATGGTTCTTATCA
TAGCGAGCGATTAGGATCGGATCCGAATAGCTGA

Upstream 100 bases:

>100_bases
CGAGCGGGGGCTTATTAAAATGCAAGGGCGGAAAGAGTTTCTTTCTGCTTTTTTATTGTCTTGATAGAATAAAAGCTTCT
TTTCAATTAGGTATCGGCTT

Downstream 100 bases:

>100_bases
ATATATTTAAATTAAGATCGTGCGGATCTTTCTCGCCGAATAAATCAGTACTAATGTTTACTTTTTCAGCAAAAAGTATG
AAGTTTGTGTGATGTTCTTA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKTIFITGGAGFIGSALIRYVIKHTDCIAVNIDKLTYAGNLASLASVSNSSRYYFEQVDICDSGRISQLFCKYQPDAVMH
LAAESHVDRSIDGPAAFMQTNIIGTYTLLEASRRYWLDLSREKKQDFRFHHISTDEVYGDLGHDKGLFTETSPYAPSSPY
SASKASSDHLVRAWFRTYGLPTLVTNCSNNYGPYQFPEKLIPLMILNALEGKSLPVYGNGLQIRDWLFVEDHVRALYKVL
CDGKVGETYNIGGHNEKTNIEVVRAICRILDELVPNKRNGINKYEELITYVKDRPGHDVRYAIDASKICTDLGWQPQETF
ESGLRKTVEWYLSNKDWWQSILNGSYHSERLGSDPNS

Sequences:

>Translated_357_residues
MKTIFITGGAGFIGSALIRYVIKHTDCIAVNIDKLTYAGNLASLASVSNSSRYYFEQVDICDSGRISQLFCKYQPDAVMH
LAAESHVDRSIDGPAAFMQTNIIGTYTLLEASRRYWLDLSREKKQDFRFHHISTDEVYGDLGHDKGLFTETSPYAPSSPY
SASKASSDHLVRAWFRTYGLPTLVTNCSNNYGPYQFPEKLIPLMILNALEGKSLPVYGNGLQIRDWLFVEDHVRALYKVL
CDGKVGETYNIGGHNEKTNIEVVRAICRILDELVPNKRNGINKYEELITYVKDRPGHDVRYAIDASKICTDLGWQPQETF
ESGLRKTVEWYLSNKDWWQSILNGSYHSERLGSDPNS
>Mature_357_residues
MKTIFITGGAGFIGSALIRYVIKHTDCIAVNIDKLTYAGNLASLASVSNSSRYYFEQVDICDSGRISQLFCKYQPDAVMH
LAAESHVDRSIDGPAAFMQTNIIGTYTLLEASRRYWLDLSREKKQDFRFHHISTDEVYGDLGHDKGLFTETSPYAPSSPY
SASKASSDHLVRAWFRTYGLPTLVTNCSNNYGPYQFPEKLIPLMILNALEGKSLPVYGNGLQIRDWLFVEDHVRALYKVL
CDGKVGETYNIGGHNEKTNIEVVRAICRILDELVPNKRNGINKYEELITYVKDRPGHDVRYAIDASKICTDLGWQPQETF
ESGLRKTVEWYLSNKDWWQSILNGSYHSERLGSDPNS

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=36.3636363636364, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=25.3731343283582, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI56237023, Length=359, Percent_Identity=25.9052924791086, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI56118217, Length=359, Percent_Identity=25.9052924791086, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI189083684, Length=359, Percent_Identity=25.9052924791086, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=72.1590909090909, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=69.1218130311615, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI1788366, Length=193, Percent_Identity=29.5336787564767, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=37.4251497005988, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=35.6495468277946, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17539532, Length=344, Percent_Identity=24.4186046511628, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI133901790, Length=186, Percent_Identity=27.4193548387097, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=186, Percent_Identity=27.4193548387097, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=186, Percent_Identity=27.4193548387097, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=186, Percent_Identity=27.4193548387097, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=186, Percent_Identity=27.4193548387097, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=281, Percent_Identity=23.8434163701068, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=371, Percent_Identity=25.8760107816712, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356223, Length=350, Percent_Identity=24, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24158427, Length=339, Percent_Identity=24.188790560472, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI19923002, Length=367, Percent_Identity=25.0681198910082, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40410; Mature: 40410

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIFITGGAGFIGSALIRYVIKHTDCIAVNIDKLTYAGNLASLASVSNSSRYYFEQVDI
CEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCEEECCCHHHHHHCCCCCCEEEEEECC
CDSGRISQLFCKYQPDAVMHLAAESHVDRSIDGPAAFMQTNIIGTYTLLEASRRYWLDLS
CCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHEECCC
REKKQDFRFHHISTDEVYGDLGHDKGLFTETSPYAPSSPYSASKASSDHLVRAWFRTYGL
HHHHHCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PTLVTNCSNNYGPYQFPEKLIPLMILNALEGKSLPVYGNGLQIRDWLFVEDHVRALYKVL
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCEEEEEEEEHHHHHHHHHHHH
CDGKVGETYNIGGHNEKTNIEVVRAICRILDELVPNKRNGINKYEELITYVKDRPGHDVR
CCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEE
YAIDASKICTDLGWQPQETFESGLRKTVEWYLSNKDWWQSILNGSYHSERLGSDPNS
EEECHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCCCCCC
>Mature Secondary Structure
MKTIFITGGAGFIGSALIRYVIKHTDCIAVNIDKLTYAGNLASLASVSNSSRYYFEQVDI
CEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCEEECCCHHHHHHCCCCCCEEEEEECC
CDSGRISQLFCKYQPDAVMHLAAESHVDRSIDGPAAFMQTNIIGTYTLLEASRRYWLDLS
CCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHEECCC
REKKQDFRFHHISTDEVYGDLGHDKGLFTETSPYAPSSPYSASKASSDHLVRAWFRTYGL
HHHHHCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PTLVTNCSNNYGPYQFPEKLIPLMILNALEGKSLPVYGNGLQIRDWLFVEDHVRALYKVL
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCEEEEEEEEHHHHHHHHHHHH
CDGKVGETYNIGGHNEKTNIEVVRAICRILDELVPNKRNGINKYEELITYVKDRPGHDVR
CCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEE
YAIDASKICTDLGWQPQETFESGLRKTVEWYLSNKDWWQSILNGSYHSERLGSDPNS
EEECHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]