Click here to switch to the map view.

The map label for this gene is napA [H]

Identifier: 126208893

GI number: 126208893

Start: 1634007

End: 1636490

Strand: Reverse

Name: napA [H]

Synonym: APL_1429

Alternate gene names: 126208893

Gene position: 1636490-1634007 (Counterclockwise)

Preceding gene: 126208894

Following gene: 126208892

Centisome position: 71.95

GC content: 45.49

Gene sequence:

>2484_bases
ATGGAACTCAATCGTAGAGATTTTATGAAAGCAAATGCGGCGATGGCTGCGGCTGCTGCGGCAGGAATGACGATTCCTGT
AAAAAATGTTTATGCTGCTGATGACGGTATTAGATGGGATAAAGCTCCATGCCGTTTCTGCGGTACGGGTTGTAGCGTAC
TGGTCGGTACCAAAGACGGTCGTGTGGTTGCGACCCAAGGTGACCCGGATGCGGAAGTAAACCGCGGTTTAAACTGTATT
AAAGGTTACTTCTTATCAAAAATTATGTACGGCGCAGACCGTGTACAAACGCCTTTATTACGTATGAAAGACGGTAAATT
CCATAAAGAAGGTGACTTTACCCCGGTTTCTTGGGATCAGGCTTTCACCATTATGGCGGAGAAAGTAAAAGACATTCTGA
AGAAAAAAGAACCGAATTCGGTCGGTATGTTCTCATCAGGTCAAACTACTATTTTTGAAGGCTATGCGAAAGTTAAACTT
TGGAAAGGAGGTTTACGTTCTAACACTATCGACCCGAATGCGCGTCACTGTATGGCGTCTGCGGCGGTAGCCTTTATGCG
TACTTTCGGTATGGACGAACCGATGGGCTGCTATAACGATATTGAGAAAACCGACGCGTTCGTGCTTTGGGGTTCGAATA
TGGCGGAAATGCACCCGATTTTATGGAGCCGTATTTCCGACCGCCGCTTATCGGACGATAAAGTGAAAGTGGTGGTAATG
TCCACATTTGAACACCGTTCATTTGAATTGGCGGATACCCCGATTATCTTTAAACCGCATTCAGATTTAGCAATTCTTAA
CTATATCGCCAACTACATTATCCAAAACGATAAAGTAAACTGGGATTTCGTTAATAAACATACTAAATTTAAACGCGGCG
AAACGGATATCGGTTACGGTTTACGTCCGGATCATCCTCGCCAAAAAGCGGCGAAAAATGCGAAAACCGCCGGCAAAATG
TATGATTCCGATTTCGAAGAATTTAAGAAAATCGTTGAGCCTTATACATTAGAAAAAGCGCACGAGATTTCCGGCGTACC
GAAAGATCAGTTGGAAACGCTGGCGAAAATGTATGCGGATCCGAAACAAACGTTAGTATCTTTTTGGACAATGGGCTTTA
ACCAGCACGTACGCGGCGTATGGGTAAACCACATGGTTTATAACGTACACTTGCTTACCGGTAAAATCTCAACGCCGGGT
TGCGGTCCGTTCTCATTAACCGGTCAGCCGTCAGCTTGCGGTACGGCGCGCGAAGTCGGTACTTTCGTACACCGTTTACC
GGCGGACATGGTGGTAACCAATCCGAAACACGTTGAAATTGTAGAAAAAGCGTGGAAATTACCGAAAGGTACGATTCCGA
CCGTGCCGGGGTATCCTGCGGTTATGCAAAGCCGTATGTTAAAAGACGGTAAACTTAATTTCTTATGGCAGCTTTGTACC
AACAATATGCAAGGCGGTCCGAATATTAATGAAGAGATCTTCCCGGGTTGGCGTAATCCTGAAAACTTTATTGTGGTTTC
CGATCCGTATCCTTCTGTTTCTGCGGTAGCTGCGGACTTAATTCTTCCGACTTGTATGTGGGTGGAAAAAGAAGGGGCTT
ACGGTAATGCGGAACGTCGTACTCAATTCTGGCGTCAGCAAGTGAAAGGTCCGGGCGAGTCAAAATCAGACTTATGGCAA
ATCGTTGAATTCTCTAAATACTTCAAAACCGACGAAGTATGGGATGAAGCGTTACTTGCACAAATGCCGGAATATCGCGG
TAAAACTCTCTACGAAGTGCTGTATAAAAACGGTCAAGTGGATAAATTCGATACGCCGACTAACATACCGGGTTATATCA
ATGATGAAGCGGAACACTTCGGTTACTACTTACAAAAAGGTTTATTTGAAGAATATGCGGCATTCGGTCGCGGTCACGGA
CATGACTTAGCCGACTTTGATACTTACCATCAAGTGCGCGGTTTACGTTGGCCGGTAGTGGACGGTAAAGAAACCTTATG
GCGTTACCGTGAAGGCTTCGACCCGTACGTCAAAGCGGGTGAAGGCGTATCGTTCTACGGCTATCCGGATAAGAAAGCGA
TTATCTTAGGCGTACCTTACGAAGAGCCAGCGGAAAGTCCGGACGAAGAGTACGATTTATGGTTATGTACCGGTCGTGTT
CTTGAACATTGGCATACAGGTACGATGACACGTCGTGTACCGGAATTACACCGTTCGTTCCCGAATAACCTTTGCTGGAT
GCATCCGGACGATGCTAAGGCTCGCGGGTTACGTCACGGTGATAAAGTGAAATTAATTACTCGTCGCGGTGAAATTATTA
CTCACTTAGATACGCGCGGTCGTAATAAATGTCCGAAAGGCTTAATTTATACCACCTTCTTCGATGCGGGGCAGTTAGCC
AATAAATTAACTTTAGATGCAACCGACCCGATTTCAGGTGAAACCGACTATAAAAAATGTGCGGTTAAAGTGGTTAAGGC
GTAA

Upstream 100 bases:

>100_bases
GATCAGGTAAAGGATATTGACGGTGTGATTGTCGTATTGTTAATTTCCAATTATCTGGATGAACAATAGTTTTAATTTTT
TTATAAAAGTGGGAAACATT

Downstream 100 bases:

>100_bases
TGAAACATACGGTCGGAATTTACAAAGTTTTTTGAAAATCCGGCCGCTTGTAGGGTGAGTATGCTTGCTTACCAATATAA
TTTACGTCGTTAAAGAAGCG

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 827; Mature: 827

Protein sequence:

>827_residues
MELNRRDFMKANAAMAAAAAAGMTIPVKNVYAADDGIRWDKAPCRFCGTGCSVLVGTKDGRVVATQGDPDAEVNRGLNCI
KGYFLSKIMYGADRVQTPLLRMKDGKFHKEGDFTPVSWDQAFTIMAEKVKDILKKKEPNSVGMFSSGQTTIFEGYAKVKL
WKGGLRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMHPILWSRISDRRLSDDKVKVVVM
STFEHRSFELADTPIIFKPHSDLAILNYIANYIIQNDKVNWDFVNKHTKFKRGETDIGYGLRPDHPRQKAAKNAKTAGKM
YDSDFEEFKKIVEPYTLEKAHEISGVPKDQLETLAKMYADPKQTLVSFWTMGFNQHVRGVWVNHMVYNVHLLTGKISTPG
CGPFSLTGQPSACGTAREVGTFVHRLPADMVVTNPKHVEIVEKAWKLPKGTIPTVPGYPAVMQSRMLKDGKLNFLWQLCT
NNMQGGPNINEEIFPGWRNPENFIVVSDPYPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPGESKSDLWQ
IVEFSKYFKTDEVWDEALLAQMPEYRGKTLYEVLYKNGQVDKFDTPTNIPGYINDEAEHFGYYLQKGLFEEYAAFGRGHG
HDLADFDTYHQVRGLRWPVVDGKETLWRYREGFDPYVKAGEGVSFYGYPDKKAIILGVPYEEPAESPDEEYDLWLCTGRV
LEHWHTGTMTRRVPELHRSFPNNLCWMHPDDAKARGLRHGDKVKLITRRGEIITHLDTRGRNKCPKGLIYTTFFDAGQLA
NKLTLDATDPISGETDYKKCAVKVVKA

Sequences:

>Translated_827_residues
MELNRRDFMKANAAMAAAAAAGMTIPVKNVYAADDGIRWDKAPCRFCGTGCSVLVGTKDGRVVATQGDPDAEVNRGLNCI
KGYFLSKIMYGADRVQTPLLRMKDGKFHKEGDFTPVSWDQAFTIMAEKVKDILKKKEPNSVGMFSSGQTTIFEGYAKVKL
WKGGLRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMHPILWSRISDRRLSDDKVKVVVM
STFEHRSFELADTPIIFKPHSDLAILNYIANYIIQNDKVNWDFVNKHTKFKRGETDIGYGLRPDHPRQKAAKNAKTAGKM
YDSDFEEFKKIVEPYTLEKAHEISGVPKDQLETLAKMYADPKQTLVSFWTMGFNQHVRGVWVNHMVYNVHLLTGKISTPG
CGPFSLTGQPSACGTAREVGTFVHRLPADMVVTNPKHVEIVEKAWKLPKGTIPTVPGYPAVMQSRMLKDGKLNFLWQLCT
NNMQGGPNINEEIFPGWRNPENFIVVSDPYPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPGESKSDLWQ
IVEFSKYFKTDEVWDEALLAQMPEYRGKTLYEVLYKNGQVDKFDTPTNIPGYINDEAEHFGYYLQKGLFEEYAAFGRGHG
HDLADFDTYHQVRGLRWPVVDGKETLWRYREGFDPYVKAGEGVSFYGYPDKKAIILGVPYEEPAESPDEEYDLWLCTGRV
LEHWHTGTMTRRVPELHRSFPNNLCWMHPDDAKARGLRHGDKVKLITRRGEIITHLDTRGRNKCPKGLIYTTFFDAGQLA
NKLTLDATDPISGETDYKKCAVKVVKA
>Mature_827_residues
MELNRRDFMKANAAMAAAAAAGMTIPVKNVYAADDGIRWDKAPCRFCGTGCSVLVGTKDGRVVATQGDPDAEVNRGLNCI
KGYFLSKIMYGADRVQTPLLRMKDGKFHKEGDFTPVSWDQAFTIMAEKVKDILKKKEPNSVGMFSSGQTTIFEGYAKVKL
WKGGLRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMHPILWSRISDRRLSDDKVKVVVM
STFEHRSFELADTPIIFKPHSDLAILNYIANYIIQNDKVNWDFVNKHTKFKRGETDIGYGLRPDHPRQKAAKNAKTAGKM
YDSDFEEFKKIVEPYTLEKAHEISGVPKDQLETLAKMYADPKQTLVSFWTMGFNQHVRGVWVNHMVYNVHLLTGKISTPG
CGPFSLTGQPSACGTAREVGTFVHRLPADMVVTNPKHVEIVEKAWKLPKGTIPTVPGYPAVMQSRMLKDGKLNFLWQLCT
NNMQGGPNINEEIFPGWRNPENFIVVSDPYPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPGESKSDLWQ
IVEFSKYFKTDEVWDEALLAQMPEYRGKTLYEVLYKNGQVDKFDTPTNIPGYINDEAEHFGYYLQKGLFEEYAAFGRGHG
HDLADFDTYHQVRGLRWPVVDGKETLWRYREGFDPYVKAGEGVSFYGYPDKKAIILGVPYEEPAESPDEEYDLWLCTGRV
LEHWHTGTMTRRVPELHRSFPNNLCWMHPDDAKARGLRHGDKVKLITRRGEIITHLDTRGRNKCPKGLIYTTFFDAGQLA
NKLTLDATDPISGETDYKKCAVKVVKA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=828, Percent_Identity=70.6521739130435, Blast_Score=1235, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=795, Percent_Identity=24.5283018867925, Blast_Score=205, Evalue=8e-54,
Organism=Escherichia coli, GI3868719, Length=487, Percent_Identity=23.4086242299795, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI3868720, Length=667, Percent_Identity=22.4887556221889, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081797, Length=837, Percent_Identity=22.1027479091995, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI171474008, Length=555, Percent_Identity=23.0630630630631, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 93573; Mature: 93573

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNRRDFMKANAAMAAAAAAGMTIPVKNVYAADDGIRWDKAPCRFCGTGCSVLVGTKDG
CCCCHHHHHHHHHHHHHHHHCCCEEECCEEEECCCCCCCCCCCHHHHCCCCEEEEECCCC
RVVATQGDPDAEVNRGLNCIKGYFLSKIMYGADRVQTPLLRMKDGKFHKEGDFTPVSWDQ
EEEEECCCCCHHHHHCHHHHHHHHHHHHHHCHHHHHCCHHEECCCCCCCCCCCCCCCHHH
AFTIMAEKVKDILKKKEPNSVGMFSSGQTTIFEGYAKVKLWKGGLRSNTIDPNARHCMAS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECEEEEEEECCCCCCCCCCCCHHHHHHH
AAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMHPILWSRISDRRLSDDKVKVVVM
HHHHHHHHHCCCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEE
STFEHRSFELADTPIIFKPHSDLAILNYIANYIIQNDKVNWDFVNKHTKFKRGETDIGYG
ECCCCCCEEECCCCEEEECCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCC
LRPDHPRQKAAKNAKTAGKMYDSDFEEFKKIVEPYTLEKAHEISGVPKDQLETLAKMYAD
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCC
PKQTLVSFWTMGFNQHVRGVWVNHMVYNVHLLTGKISTPGCGPFSLTGQPSACGTAREVG
HHHHHHHHHHHCCCCCHHHEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
TFVHRLPADMVVTNPKHVEIVEKAWKLPKGTIPTVPGYPAVMQSRMLKDGKLNFLWQLCT
HHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHC
NNMQGGPNINEEIFPGWRNPENFIVVSDPYPSVSAVAADLILPTCMWVEKEGAYGNAERR
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHH
TQFWRQQVKGPGESKSDLWQIVEFSKYFKTDEVWDEALLAQMPEYRGKTLYEVLYKNGQV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCHHHHHHHCCCCC
DKFDTPTNIPGYINDEAEHFGYYLQKGLFEEYAAFGRGHGHDLADFDTYHQVRGLRWPVV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCC
DGKETLWRYREGFDPYVKAGEGVSFYGYPDKKAIILGVPYEEPAESPDEEYDLWLCTGRV
CCHHHHHHHHHCCCHHHHCCCCCEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHH
LEHWHTGTMTRRVPELHRSFPNNLCWMHPDDAKARGLRHGDKVKLITRRGEIITHLDTRG
HHHHCCCCHHHHHHHHHHCCCCCEEEECCCCHHHCCCCCCCEEEEEEECCCEEEEECCCC
RNKCPKGLIYTTFFDAGQLANKLTLDATDPISGETDYKKCAVKVVKA
CCCCCCCEEEEEECCHHHHHCEEEECCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MELNRRDFMKANAAMAAAAAAGMTIPVKNVYAADDGIRWDKAPCRFCGTGCSVLVGTKDG
CCCCHHHHHHHHHHHHHHHHCCCEEECCEEEECCCCCCCCCCCHHHHCCCCEEEEECCCC
RVVATQGDPDAEVNRGLNCIKGYFLSKIMYGADRVQTPLLRMKDGKFHKEGDFTPVSWDQ
EEEEECCCCCHHHHHCHHHHHHHHHHHHHHCHHHHHCCHHEECCCCCCCCCCCCCCCHHH
AFTIMAEKVKDILKKKEPNSVGMFSSGQTTIFEGYAKVKLWKGGLRSNTIDPNARHCMAS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECEEEEEEECCCCCCCCCCCCHHHHHHH
AAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMHPILWSRISDRRLSDDKVKVVVM
HHHHHHHHHCCCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEE
STFEHRSFELADTPIIFKPHSDLAILNYIANYIIQNDKVNWDFVNKHTKFKRGETDIGYG
ECCCCCCEEECCCCEEEECCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCC
LRPDHPRQKAAKNAKTAGKMYDSDFEEFKKIVEPYTLEKAHEISGVPKDQLETLAKMYAD
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCC
PKQTLVSFWTMGFNQHVRGVWVNHMVYNVHLLTGKISTPGCGPFSLTGQPSACGTAREVG
HHHHHHHHHHHCCCCCHHHEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
TFVHRLPADMVVTNPKHVEIVEKAWKLPKGTIPTVPGYPAVMQSRMLKDGKLNFLWQLCT
HHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHC
NNMQGGPNINEEIFPGWRNPENFIVVSDPYPSVSAVAADLILPTCMWVEKEGAYGNAERR
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHH
TQFWRQQVKGPGESKSDLWQIVEFSKYFKTDEVWDEALLAQMPEYRGKTLYEVLYKNGQV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCHHHHHHHCCCCC
DKFDTPTNIPGYINDEAEHFGYYLQKGLFEEYAAFGRGHGHDLADFDTYHQVRGLRWPVV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCC
DGKETLWRYREGFDPYVKAGEGVSFYGYPDKKAIILGVPYEEPAESPDEEYDLWLCTGRV
CCHHHHHHHHHCCCHHHHCCCCCEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHH
LEHWHTGTMTRRVPELHRSFPNNLCWMHPDDAKARGLRHGDKVKLITRRGEIITHLDTRG
HHHHCCCCHHHHHHHHHHCCCCCEEEECCCCHHHCCCCCCCEEEEEEECCCEEEEECCCC
RNKCPKGLIYTTFFDAGQLANKLTLDATDPISGETDYKKCAVKVVKA
CCCCCCCEEEEEECCHHHHHCEEEECCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA