| Definition | Actinobacillus pleuropneumoniae serovar 5b str. L20 chromosome, complete genome. |
|---|---|
| Accession | NC_009053 |
| Length | 2,274,482 |
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The map label for this gene is gshA [H]
Identifier: 126208872
GI number: 126208872
Start: 1609024
End: 1611297
Strand: Direct
Name: gshA [H]
Synonym: APL_1408
Alternate gene names: 126208872
Gene position: 1609024-1611297 (Clockwise)
Preceding gene: 126208871
Following gene: 126208874
Centisome position: 70.74
GC content: 42.66
Gene sequence:
>2274_bases ATGAAATTACAACAACTGATTAAAACTCATCACCTTGGTTTACTATTTCAACAAGGTAAATTTGGCATCGAAAAAGAAAG CCAACGCATTGATAATAAAGGGAATATTGTTACTACCGCCCATCCTAGCGTTTTTGGTAACCGCAGTTATCATCCGTATA TTCAAACCGATTTTGCAGAAAGTCAGTTAGAACTTATCACACCGCCAAACGATAAATTGGAAAACACATATCGTTGGCTA TCCGCTATTCACGAGGTAACGCTACGTTCATTACCTGATGATGAATATATTTTCCCATTCAGTATGCCTGCCGGTTTACC ACCGGAATCCGAGATCAAAGAAGCACAATTAGATAACGAATGGGACGTGAAATATCGTGAACACCTTTCTGCCATTTATG GCAAATACAAACAGATGGTGAGCGGTATTCATTACAATTTCCAGATTTCTGACGAGTTTGTCGAGAGTGCTTTTGCGTTA CAAACGGAATACCCCAATAAAATTGCGTTCCGCAATGCGCTATATATGAAATTAGCCAATAACTTTTTACGTTATCAATG GATTTTAGTTTATTTGCTTGCCGCAACACCAACCGTAGAAGCGCAATATTTTGGCGAAAATCGACCGCTTGCAGGAGGGC AATTAGTACGTAGTTTACGTTCCGGCCCTTATGGTTATGTAAATGCGCCACATATTGTGATCAACCACGACAGCTTACAA CAATATGTCGAGTCGCTAGAACATTTTGTAGCAACCGGCGATTTGTTGGCAGAAAAAGAATTCTATTCAAACGTTCGTTT ACGTGGTGCGAAAAAAGCACGCGAATTGCTTGAGAAAGGAGTTAAATATGCGGAATTCCGTTTATTTGATCTTAATCCGT TCTCGCCTTACGGTATCGAGCTTGCAGACGCGAAATTTATTCATCTGTTCTTACTTGCGATGTTGTGGATGGATGAAACA AGCGGTCAAAGAGAAGTCGAAATCGGCACACAAAAACTATACCAAGTTGCCCTTGAAGATCCTCGTTCGCACACTGCGTT CCAAGCAGAGGGTGAGGCGATCCTTAACCTGATGCTGGCAATGCTGGACGATCTTTCTGTACCACAAAACGAGAAAGATT TATTACAACAAAAACTGGCGCAATTTGCCGATCCTAGTCAAACGGTAAACGGTCGTTTATTAGCCGCAGTTGAACAAGCC GGCAGCTATAAAGCACTCGGTGCACAACTTGCTCAACAATATAAAGCGCAAGCATTCGAGCGTTTTTATGCGATTTCCGC TTTCGATAATATGGAGCTTTCTACACAGGCTTTGTTATTTGATGCGATCCAACAAGGCTTACAGATCGAATTGCTTGATG AAAATGATCAGTTCCTCGCACTCAAATTCGGCGATCATCTCGAATATGTGAAAAACGGTAATATGACCAGCCACGATCAG TATATTTCGCCATTAATTATGGAAAACAAAGTCGTGACCAAAAAAGTATTGGCGAAAGCCGGTTTTAATGTGCCGAAAAG CGTTGAATTTACTTCAGTAGAACAAGCGGTGGCACACTATCCGTTATTTGAAGGTAAAGCGGTGGTAATTAAGCCGAAAT CAACTAATTACGGCTTGGGTATTACAATTTTCCAGCAAGGCGTGACGGATAAAGCCGACTTTGCCAAAGCGATTGAGATT GCGTTCCGTGAAGATAAAGAAGTGATGGTGGAAGACTATTTAGTCGGCACCGAATACCGTTTCTTTGTGTTAGGCGATGA AACACTGGCGGTATTGTTACGTGTGCCGGCAAATGTGAAAGGTGACGGCATACATACAGTGCGTGAATTGGTGGAAGCGA AAAACAGTGATCCGCTACGAGGTGACGGCTCTCGTTCACCATTGAAGAAAATCGCCCTCGGTGACATTGAATTGCTTCAG CTTAAAGAGCAAGGTTTAACGCCTGATTCGATTCCGGCTGACGGGCAAATCGTACAATTACGTGCCAACTCTAATATTAG TACCGGCGGCGATTCAATCGATATGACCGATCAAATGCATGACAGTTATAAACAATTAGCGGTCGGTCTTGCCAAAGAGA TGGGGGCAAAAGTCTGCGGTGTGGATTTAATCATTCCGGATTTAACCAAAGCCGCCGAGCCGTCTCTGCGTTCATGGGGT GTGATTGAAGCAAACTTTAATCCGATGATGATGATGCATATTTTCCCTTACCAAGGAAAATCTCGCCGCTTAACCAAAGC CGTGTTAAAAATGCTGTTCCCAGAACTGCCTTAA
Upstream 100 bases:
>100_bases TCATCTACCACCTTATCAATCGGCTATAAAACCAATCAAAAAAGTATGTTATTTTAGGGACGGGACTACGTCTAACGCAT TTGTCGCGAAAGGGAAAGCT
Downstream 100 bases:
>100_bases TCAAAAATCAAGCGGTCAAATTTGCAGAAAATTTTGCAAATTCGACCGCTTGTAACATGATAGATATCGCTAAAATAGTG AAAAGTAAGGGTTAATACTT
Product: bifunctional glutamate--cysteine ligase/glutathione synthetase
Products: NA
Alternate protein names: Gamma-GCS-GS; GCS-GS; Glutamate--cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Glutathione synthetase; GSH synthetase; GS; GSH-S; GSHase; Glutathione synthase [H]
Number of amino acids: Translated: 757; Mature: 757
Protein sequence:
>757_residues MKLQQLIKTHHLGLLFQQGKFGIEKESQRIDNKGNIVTTAHPSVFGNRSYHPYIQTDFAESQLELITPPNDKLENTYRWL SAIHEVTLRSLPDDEYIFPFSMPAGLPPESEIKEAQLDNEWDVKYREHLSAIYGKYKQMVSGIHYNFQISDEFVESAFAL QTEYPNKIAFRNALYMKLANNFLRYQWILVYLLAATPTVEAQYFGENRPLAGGQLVRSLRSGPYGYVNAPHIVINHDSLQ QYVESLEHFVATGDLLAEKEFYSNVRLRGAKKARELLEKGVKYAEFRLFDLNPFSPYGIELADAKFIHLFLLAMLWMDET SGQREVEIGTQKLYQVALEDPRSHTAFQAEGEAILNLMLAMLDDLSVPQNEKDLLQQKLAQFADPSQTVNGRLLAAVEQA GSYKALGAQLAQQYKAQAFERFYAISAFDNMELSTQALLFDAIQQGLQIELLDENDQFLALKFGDHLEYVKNGNMTSHDQ YISPLIMENKVVTKKVLAKAGFNVPKSVEFTSVEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQGVTDKADFAKAIEI AFREDKEVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVKGDGIHTVRELVEAKNSDPLRGDGSRSPLKKIALGDIELLQ LKEQGLTPDSIPADGQIVQLRANSNISTGGDSIDMTDQMHDSYKQLAVGLAKEMGAKVCGVDLIIPDLTKAAEPSLRSWG VIEANFNPMMMMHIFPYQGKSRRLTKAVLKMLFPELP
Sequences:
>Translated_757_residues MKLQQLIKTHHLGLLFQQGKFGIEKESQRIDNKGNIVTTAHPSVFGNRSYHPYIQTDFAESQLELITPPNDKLENTYRWL SAIHEVTLRSLPDDEYIFPFSMPAGLPPESEIKEAQLDNEWDVKYREHLSAIYGKYKQMVSGIHYNFQISDEFVESAFAL QTEYPNKIAFRNALYMKLANNFLRYQWILVYLLAATPTVEAQYFGENRPLAGGQLVRSLRSGPYGYVNAPHIVINHDSLQ QYVESLEHFVATGDLLAEKEFYSNVRLRGAKKARELLEKGVKYAEFRLFDLNPFSPYGIELADAKFIHLFLLAMLWMDET SGQREVEIGTQKLYQVALEDPRSHTAFQAEGEAILNLMLAMLDDLSVPQNEKDLLQQKLAQFADPSQTVNGRLLAAVEQA GSYKALGAQLAQQYKAQAFERFYAISAFDNMELSTQALLFDAIQQGLQIELLDENDQFLALKFGDHLEYVKNGNMTSHDQ YISPLIMENKVVTKKVLAKAGFNVPKSVEFTSVEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQGVTDKADFAKAIEI AFREDKEVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVKGDGIHTVRELVEAKNSDPLRGDGSRSPLKKIALGDIELLQ LKEQGLTPDSIPADGQIVQLRANSNISTGGDSIDMTDQMHDSYKQLAVGLAKEMGAKVCGVDLIIPDLTKAAEPSLRSWG VIEANFNPMMMMHIFPYQGKSRRLTKAVLKMLFPELP >Mature_757_residues MKLQQLIKTHHLGLLFQQGKFGIEKESQRIDNKGNIVTTAHPSVFGNRSYHPYIQTDFAESQLELITPPNDKLENTYRWL SAIHEVTLRSLPDDEYIFPFSMPAGLPPESEIKEAQLDNEWDVKYREHLSAIYGKYKQMVSGIHYNFQISDEFVESAFAL QTEYPNKIAFRNALYMKLANNFLRYQWILVYLLAATPTVEAQYFGENRPLAGGQLVRSLRSGPYGYVNAPHIVINHDSLQ QYVESLEHFVATGDLLAEKEFYSNVRLRGAKKARELLEKGVKYAEFRLFDLNPFSPYGIELADAKFIHLFLLAMLWMDET SGQREVEIGTQKLYQVALEDPRSHTAFQAEGEAILNLMLAMLDDLSVPQNEKDLLQQKLAQFADPSQTVNGRLLAAVEQA GSYKALGAQLAQQYKAQAFERFYAISAFDNMELSTQALLFDAIQQGLQIELLDENDQFLALKFGDHLEYVKNGNMTSHDQ YISPLIMENKVVTKKVLAKAGFNVPKSVEFTSVEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQGVTDKADFAKAIEI AFREDKEVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVKGDGIHTVRELVEAKNSDPLRGDGSRSPLKKIALGDIELLQ LKEQGLTPDSIPADGQIVQLRANSNISTGGDSIDMTDQMHDSYKQLAVGLAKEMGAKVCGVDLIIPDLTKAAEPSLRSWG VIEANFNPMMMMHIFPYQGKSRRLTKAVLKMLFPELP
Specific function: Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine [H]
COG id: COG2918
COG function: function code H; Gamma-glutamylcysteine synthetase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ATP-grasp domain [H]
Homologues:
Organism=Escherichia coli, GI1789044, Length=454, Percent_Identity=25.3303964757709, Blast_Score=144, Evalue=2e-35,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011761 - InterPro: IPR013815 - InterPro: IPR013816 - InterPro: IPR011095 - InterPro: IPR007370 - InterPro: IPR006335 [H]
Pfam domain/function: PF07478 Dala_Dala_lig_C; PF04262 Glu_cys_ligase [H]
EC number: =6.3.2.2; =6.3.2.3 [H]
Molecular weight: Translated: 85252; Mature: 85252
Theoretical pI: Translated: 5.37; Mature: 5.37
Prosite motif: PS50975 ATP_GRASP
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKLQQLIKTHHLGLLFQQGKFGIEKESQRIDNKGNIVTTAHPSVFGNRSYHPYIQTDFAE CCHHHHHHHHHHHEEEECCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCCCCEECCCCC SQLELITPPNDKLENTYRWLSAIHEVTLRSLPDDEYIFPFSMPAGLPPESEIKEAQLDNE CCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHCCCC WDVKYREHLSAIYGKYKQMVSGIHYNFQISDEFVESAFALQTEYPNKIAFRNALYMKLAN CCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH NFLRYQWILVYLLAATPTVEAQYFGENRPLAGGQLVRSLRSGPYGYVNAPHIVINHDSLQ HHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECHHHH QYVESLEHFVATGDLLAEKEFYSNVRLRGAKKARELLEKGVKYAEFRLFDLNPFSPYGIE HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCEE LADAKFIHLFLLAMLWMDETSGQREVEIGTQKLYQVALEDPRSHTAFQAEGEAILNLMLA ECHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH MLDDLSVPQNEKDLLQQKLAQFADPSQTVNGRLLAAVEQAGSYKALGAQLAQQYKAQAFE HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHH RFYAISAFDNMELSTQALLFDAIQQGLQIELLDENDQFLALKFGDHLEYVKNGNMTSHDQ HHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCHHHHHHCCCCCCCHH YISPLIMENKVVTKKVLAKAGFNVPKSVEFTSVEQAVAHYPLFEGKAVVIKPKSTNYGLG HHCHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCE ITIFQQGVTDKADFAKAIEIAFREDKEVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVK EEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHEEECCCEEEEEECCHHHEEEEECCCCCC GDGIHTVRELVEAKNSDPLRGDGSRSPLKKIALGDIELLQLKEQGLTPDSIPADGQIVQL CCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEE RANSNISTGGDSIDMTDQMHDSYKQLAVGLAKEMGAKVCGVDLIIPDLTKAAEPSLRSWG EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHCCCCCCCCE VIEANFNPMMMMHIFPYQGKSRRLTKAVLKMLFPELP EEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCC >Mature Secondary Structure MKLQQLIKTHHLGLLFQQGKFGIEKESQRIDNKGNIVTTAHPSVFGNRSYHPYIQTDFAE CCHHHHHHHHHHHEEEECCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCCCCEECCCCC SQLELITPPNDKLENTYRWLSAIHEVTLRSLPDDEYIFPFSMPAGLPPESEIKEAQLDNE CCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHCCCC WDVKYREHLSAIYGKYKQMVSGIHYNFQISDEFVESAFALQTEYPNKIAFRNALYMKLAN CCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH NFLRYQWILVYLLAATPTVEAQYFGENRPLAGGQLVRSLRSGPYGYVNAPHIVINHDSLQ HHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECHHHH QYVESLEHFVATGDLLAEKEFYSNVRLRGAKKARELLEKGVKYAEFRLFDLNPFSPYGIE HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCEE LADAKFIHLFLLAMLWMDETSGQREVEIGTQKLYQVALEDPRSHTAFQAEGEAILNLMLA ECHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH MLDDLSVPQNEKDLLQQKLAQFADPSQTVNGRLLAAVEQAGSYKALGAQLAQQYKAQAFE HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHH RFYAISAFDNMELSTQALLFDAIQQGLQIELLDENDQFLALKFGDHLEYVKNGNMTSHDQ HHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCHHHHHHCCCCCCCHH YISPLIMENKVVTKKVLAKAGFNVPKSVEFTSVEQAVAHYPLFEGKAVVIKPKSTNYGLG HHCHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCE ITIFQQGVTDKADFAKAIEIAFREDKEVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVK EEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHEEECCCEEEEEECCHHHEEEEECCCCCC GDGIHTVRELVEAKNSDPLRGDGSRSPLKKIALGDIELLQLKEQGLTPDSIPADGQIVQL CCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEE RANSNISTGGDSIDMTDQMHDSYKQLAVGLAKEMGAKVCGVDLIIPDLTKAAEPSLRSWG EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHCCCCCCCCE VIEANFNPMMMMHIFPYQGKSRRLTKAVLKMLFPELP EEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA