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The map label for this gene is miaB

Identifier: 126208789

GI number: 126208789

Start: 1518127

End: 1519554

Strand: Direct

Name: miaB

Synonym: APL_1325

Alternate gene names: 126208789

Gene position: 1518127-1519554 (Clockwise)

Preceding gene: 126208788

Following gene: 126208790

Centisome position: 66.75

GC content: 45.1

Gene sequence:

>1428_bases
ATGGCGAAATTGCATATTACGACTTGGGGCTGCCAAATGAATGAGTACGACTCATCCAAAATGGCGGATCTTTTAAATTC
CACACACGGTCTAGAACTGACCGATAAACCGGAAGAAGCGGACGTTTTATTACTTAATACCTGCTCAATCCGCGAAAAAG
CGCAAGAAAAAGTCTTCTCGCAACTTGGTCGTTGGAAAAATTGGAAAAAAGATAAACCGGATCTGATTATCGGTGTCGGC
GGTTGCGTAGCGTCGCAAGAAGGCGAACATATTCGTGATCGTGCGCCGTTTGTTGATATCGTATTCGGTCCGCAAACATT
GCACCGTTTACCGGAAATGATCAACAAAATCCGTGGCGGTGACCGTGCGATCGTCGATATTTCTTTCCCGGAAATTGAAA
AATTCGACCGCTTGCCGGAACCGCGTGCCGAAGGCCCGACTGCTTTCGTTTCCATTATGGAAGGTTGTAATAAATACTGC
TCGTTCTGCGTAGTGCCTTACACTCGTGGTGAAGAAGTTTCTCGTCCGGTGGATGACGTATTATTTGAAATCGCACAATT
AGCGGAACAAGGCGTACGTGAAGTGAACTTACTCGGTCAAAACGTGAATGCTTATCGCGGTGAAACTTTTGACGGTGGAA
TTTGCACATTTGCCGAATTGCTTCGTTTAGTCGCTGCGATTGACGGTATCGACCGTGTACGCTACACCACCAGTCACCCG
ATTGAATTTACCGATGACATCATTGAAGTTTATCGCGACACGCCCGAATTAGTGAGCTTCTTACACTTACCAATTCAAAG
CGGTGCGGATCGCGTATTAACCATGATGAAACGTAACCATACGGCATTAGAATATAAAGCGATCATTCGTAAATTACGTG
AAGTTCGTCCGAATATTCAGATCAGTTCGGACTTTATCGTTGGTTTCCCGGGTGAAACGGCGGAAGATTTCGAACAAACC
ATGAAAGTAATCGAGCAAGTAAACTTTGATATGAGCTTCAGCTTTATCTACTCTGCTCGCCCGGGTACACCGGCTGCCGA
TTTACCGGATGATATTTCGGAAGAAGAGAAAAAAGAGCGTTTAGCACGTTTACAACAACGTATCAATCACCAAGCTATGC
AATTCAGTCGTGCAATGTTAGGCACGGAACAACGTGTATTGGTGGAAGGCCCGTCGAAAAAAGATATTATGGAATTAACC
GGTCGTACCGAAAACAACCGAATTGTGAACTTCCAAGGCACACCGGATATGATTGGTAAATTTGTCGATATTAAAATCAC
CGATGTTTATACCAACTCATTACGCGGCGATGTGGTTCGTACCGAAGACGAAATGGGTTTACGTGTGGTCGAATCGGCGG
CAAGCGTGATTGCTCGTACTCGTAAAGAAGACGATCTCGGCGTCGGCAAATATGTTGTAAATCTATAA

Upstream 100 bases:

>100_bases
ATAAGCAAACGGGTACAATAAACCGAATTTGTGCGTAAAGGGATTCCTCTTTACGCTTTTATTTTTAACTTATTGATATA
TTTATTATTTTCTAAAAATT

Downstream 100 bases:

>100_bases
TAGTAAATTAAGCAGATCTTATTAAACTAAGATCTGCTTATTTTTTATCCTGGCTTTACCTCTTTCAATATAATTCAATC
CTTTCGTATTTTATTCTCAT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MAKLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADVLLLNTCSIREKAQEKVFSQLGRWKNWKKDKPDLIIGVG
GCVASQEGEHIRDRAPFVDIVFGPQTLHRLPEMINKIRGGDRAIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYC
SFCVVPYTRGEEVSRPVDDVLFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRVRYTTSHP
IEFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLREVRPNIQISSDFIVGFPGETAEDFEQT
MKVIEQVNFDMSFSFIYSARPGTPAADLPDDISEEEKKERLARLQQRINHQAMQFSRAMLGTEQRVLVEGPSKKDIMELT
GRTENNRIVNFQGTPDMIGKFVDIKITDVYTNSLRGDVVRTEDEMGLRVVESAASVIARTRKEDDLGVGKYVVNL

Sequences:

>Translated_475_residues
MAKLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADVLLLNTCSIREKAQEKVFSQLGRWKNWKKDKPDLIIGVG
GCVASQEGEHIRDRAPFVDIVFGPQTLHRLPEMINKIRGGDRAIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYC
SFCVVPYTRGEEVSRPVDDVLFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRVRYTTSHP
IEFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLREVRPNIQISSDFIVGFPGETAEDFEQT
MKVIEQVNFDMSFSFIYSARPGTPAADLPDDISEEEKKERLARLQQRINHQAMQFSRAMLGTEQRVLVEGPSKKDIMELT
GRTENNRIVNFQGTPDMIGKFVDIKITDVYTNSLRGDVVRTEDEMGLRVVESAASVIARTRKEDDLGVGKYVVNL
>Mature_474_residues
AKLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADVLLLNTCSIREKAQEKVFSQLGRWKNWKKDKPDLIIGVGG
CVASQEGEHIRDRAPFVDIVFGPQTLHRLPEMINKIRGGDRAIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCS
FCVVPYTRGEEVSRPVDDVLFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRVRYTTSHPI
EFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLREVRPNIQISSDFIVGFPGETAEDFEQTM
KVIEQVNFDMSFSFIYSARPGTPAADLPDDISEEEKKERLARLQQRINHQAMQFSRAMLGTEQRVLVEGPSKKDIMELTG
RTENNRIVNFQGTPDMIGKFVDIKITDVYTNSLRGDVVRTEDEMGLRVVESAASVIARTRKEDDLGVGKYVVNL

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=37.0833333333333, Blast_Score=295, Evalue=8e-80,
Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=37.0833333333333, Blast_Score=294, Evalue=2e-79,
Organism=Homo sapiens, GI93277076, Length=446, Percent_Identity=28.0269058295964, Blast_Score=169, Evalue=4e-42,
Organism=Escherichia coli, GI1786882, Length=469, Percent_Identity=81.6631130063966, Blast_Score=810, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=393, Percent_Identity=29.7709923664122, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=34.9240780911063, Blast_Score=264, Evalue=8e-71,
Organism=Caenorhabditis elegans, GI71996771, Length=301, Percent_Identity=28.9036544850498, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356207, Length=432, Percent_Identity=36.8055555555556, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=28.7581699346405, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_ACTP2 (A3N1X3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054014.1
- ProteinModelPortal:   A3N1X3
- SMR:   A3N1X3
- STRING:   A3N1X3
- GeneID:   4850295
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1325
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   A3N1X3
- ProtClustDB:   PRK14325
- BioCyc:   APLE416269:APL_1325-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53671; Mature: 53540

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADVLLLNTCSIREKAQEKVFS
CCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
QLGRWKNWKKDKPDLIIGVGGCVASQEGEHIRDRAPFVDIVFGPQTLHRLPEMINKIRGG
HHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHCCC
DRAIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV
CEEEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHH
LFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRVRYTTSHP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHEECCCCC
IEFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLREVRPNIQ
CCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
ISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFSFIYSARPGTPAADLPDDISEEEKKER
ECCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHH
LARLQQRINHQAMQFSRAMLGTEQRVLVEGPSKKDIMELTGRTENNRIVNFQGTPDMIGK
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCEEEEECCCHHHHCC
FVDIKITDVYTNSLRGDVVRTEDEMGLRVVESAASVIARTRKEDDLGVGKYVVNL
EEEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
>Mature Secondary Structure 
AKLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADVLLLNTCSIREKAQEKVFS
CEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
QLGRWKNWKKDKPDLIIGVGGCVASQEGEHIRDRAPFVDIVFGPQTLHRLPEMINKIRGG
HHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHCCC
DRAIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV
CEEEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHH
LFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRVRYTTSHP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHEECCCCC
IEFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLREVRPNIQ
CCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
ISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFSFIYSARPGTPAADLPDDISEEEKKER
ECCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHH
LARLQQRINHQAMQFSRAMLGTEQRVLVEGPSKKDIMELTGRTENNRIVNFQGTPDMIGK
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCEEEEECCCHHHHCC
FVDIKITDVYTNSLRGDVVRTEDEMGLRVVESAASVIARTRKEDDLGVGKYVVNL
EEEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA