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The map label for this gene is ptsI [H]

Identifier: 126208787

GI number: 126208787

Start: 1515636

End: 1517357

Strand: Direct

Name: ptsI [H]

Synonym: APL_1323

Alternate gene names: 126208787

Gene position: 1515636-1517357 (Clockwise)

Preceding gene: 126208786

Following gene: 126208788

Centisome position: 66.64

GC content: 42.86

Gene sequence:

>1722_bases
ATGATTACAGGTATTGCAGCTTCTCCGGGTGTTGTGTTTGGTAAAGCGCTCGTATTAAAAGAAGAACCTATTGTACTTAA
TACCCAAAAAATCACAGCGGATCAAATTGAAGCTGAAAAAGCCAAATTCTTTGCCGGCCGTGAAAAAGCGGCGGCACAAT
TGACTGCAATTAAAGAGAAAGCAAGAGGTACTCTCGGTGAAGAAAAAGAAGCTATCTTTGAAGGTCATTTAATGATCTTG
GAAGATGAAGAGCTGGAAGAAGAAATTCTGGGCTATATTGCGGATAATCTTGTAACGGCGGATGTTGCGGCAAGTAAAGT
TATTGATATGCAGGCATCTATGTTGGCAGAAATTGATGACGAATACCTAAAAGAACGTGCCGGCGATATTCGTGATATCG
GTAACCGTTTACTACGCAATATTTTAAATATGCACATCATCGATTTAGGTGATATTCAAGAGGAAGTTATTTTAGTTGCT
TACGACTTAACGCCTTCCGAAACCGCACAATTAAACTTAGATAAAGTATTAGGTTTCATTACCGATATCGGCGGTCGTAC
TTCGCATACTTCAATTATGGCTCGTTCATTAGAACTTCCTGCAATTGTCGGTACAAACGATATTACCGCTCGTGTTAAAA
CCGGCGATACATTAATTTTGGATGCGGTAAATAACCAAATTCATATTAATCCTTCCGATGCGGCACTTGCCGAATTTAAA
GCGATTCAAGAACGCGTTGCAGCGGAAAAAGCCGAACTTGCGAAATTAAAAGATTTACCGGCGGAAACTTTAGACGGTCA
TCGTATTGAAGTGGCGGGTAACATCGGCACCATTCGTGATGTGGACGGCGTCTTACGTAACGGCGGCGAATCGGTCGGTT
TATACCGTACCGAATTCTTATTTATGGATCGTAGCGAATTACCGAGCGAAGAAGAGCAATTCCAAGCGTATAAAGAAATT
GTTGAAGCGATGGACGGTAAACAAGTTGTATTACGTACCATGGATATCGGCGGCGACAAAGAATTACCGTACTTAAACCT
ACCGAAAGAAATGAATCCGTTCTTAGGTTGGCGTGCGGTGCGTATCGGTTTAACACGTCGTGAAATCTTAGATACGCAAT
TACGTGCGGTATTACGTGCTTCGGCATTCGGTAAATTAGCGGTAATGTTCCCAATGATTATCTCGGTTGAAGAGATTCGT
GAATTAAAAGCAATCGTTGCGGAATTAAAAGAACAGCTCCGTGCGGAAGGCAAAGCATTTGATGAGAACCTACAATTAGG
TATCATGGTTGAAACGCCGTCTGCAGCGGTAAATGCTCGCCACCTAGCGAAAGAAGCGGACTTTTTCAGTATCGGTACGA
ACGACTTAACCCAATATACCCTAGCGGTTGATCGCGGTAACGAAATTATTGCGCATTTATATAATCCGTTAAGTCCGTCG
GTATTGAACTTAATCAAACAAGTGATTGACGCTTCACATGCCGAAGGTAAATGGACCGGTATGTGCGGTGAGTTGGCCGG
TGACGTTCGTGCAACCGCATTATTACTCGGTATGGGCTTAGACGAGTTCAGTATGAGTGCGATTTCCGTCCCTCACGTGA
AAAAACTTGCTCGCTCGATTAATTATGCCGATGCAAAAGCATTAGCGGATGAAGCGTTAGCGCAACCGACGGCTACGGAT
ATTGAAAAGCTGGTCAATGATTTTTACGCTAAACTGAACTAA

Upstream 100 bases:

>100_bases
TGTAAAGCAGCCCTATTGCCATTCCTATTAAAAAATAGGGATGGAATAGGGTTGTATTGTGCTATATGAGCATTTAATCA
CTTTTAGACAAGGACATATT

Downstream 100 bases:

>100_bases
TTTTCAATAAATGGCATACAAAGACAAGATTCTCGTATAGAATGTACGGGTATTTTGTCTATTATTAAAATAACGGAGAT
TTCTATGGGCTTTTTCGACA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MITGIAASPGVVFGKALVLKEEPIVLNTQKITADQIEAEKAKFFAGREKAAAQLTAIKEKARGTLGEEKEAIFEGHLMIL
EDEELEEEILGYIADNLVTADVAASKVIDMQASMLAEIDDEYLKERAGDIRDIGNRLLRNILNMHIIDLGDIQEEVILVA
YDLTPSETAQLNLDKVLGFITDIGGRTSHTSIMARSLELPAIVGTNDITARVKTGDTLILDAVNNQIHINPSDAALAEFK
AIQERVAAEKAELAKLKDLPAETLDGHRIEVAGNIGTIRDVDGVLRNGGESVGLYRTEFLFMDRSELPSEEEQFQAYKEI
VEAMDGKQVVLRTMDIGGDKELPYLNLPKEMNPFLGWRAVRIGLTRREILDTQLRAVLRASAFGKLAVMFPMIISVEEIR
ELKAIVAELKEQLRAEGKAFDENLQLGIMVETPSAAVNARHLAKEADFFSIGTNDLTQYTLAVDRGNEIIAHLYNPLSPS
VLNLIKQVIDASHAEGKWTGMCGELAGDVRATALLLGMGLDEFSMSAISVPHVKKLARSINYADAKALADEALAQPTATD
IEKLVNDFYAKLN

Sequences:

>Translated_573_residues
MITGIAASPGVVFGKALVLKEEPIVLNTQKITADQIEAEKAKFFAGREKAAAQLTAIKEKARGTLGEEKEAIFEGHLMIL
EDEELEEEILGYIADNLVTADVAASKVIDMQASMLAEIDDEYLKERAGDIRDIGNRLLRNILNMHIIDLGDIQEEVILVA
YDLTPSETAQLNLDKVLGFITDIGGRTSHTSIMARSLELPAIVGTNDITARVKTGDTLILDAVNNQIHINPSDAALAEFK
AIQERVAAEKAELAKLKDLPAETLDGHRIEVAGNIGTIRDVDGVLRNGGESVGLYRTEFLFMDRSELPSEEEQFQAYKEI
VEAMDGKQVVLRTMDIGGDKELPYLNLPKEMNPFLGWRAVRIGLTRREILDTQLRAVLRASAFGKLAVMFPMIISVEEIR
ELKAIVAELKEQLRAEGKAFDENLQLGIMVETPSAAVNARHLAKEADFFSIGTNDLTQYTLAVDRGNEIIAHLYNPLSPS
VLNLIKQVIDASHAEGKWTGMCGELAGDVRATALLLGMGLDEFSMSAISVPHVKKLARSINYADAKALADEALAQPTATD
IEKLVNDFYAKLN
>Mature_573_residues
MITGIAASPGVVFGKALVLKEEPIVLNTQKITADQIEAEKAKFFAGREKAAAQLTAIKEKARGTLGEEKEAIFEGHLMIL
EDEELEEEILGYIADNLVTADVAASKVIDMQASMLAEIDDEYLKERAGDIRDIGNRLLRNILNMHIIDLGDIQEEVILVA
YDLTPSETAQLNLDKVLGFITDIGGRTSHTSIMARSLELPAIVGTNDITARVKTGDTLILDAVNNQIHINPSDAALAEFK
AIQERVAAEKAELAKLKDLPAETLDGHRIEVAGNIGTIRDVDGVLRNGGESVGLYRTEFLFMDRSELPSEEEQFQAYKEI
VEAMDGKQVVLRTMDIGGDKELPYLNLPKEMNPFLGWRAVRIGLTRREILDTQLRAVLRASAFGKLAVMFPMIISVEEIR
ELKAIVAELKEQLRAEGKAFDENLQLGIMVETPSAAVNARHLAKEADFFSIGTNDLTQYTLAVDRGNEIIAHLYNPLSPS
VLNLIKQVIDASHAEGKWTGMCGELAGDVRATALLLGMGLDEFSMSAISVPHVKKLARSINYADAKALADEALAQPTATD
IEKLVNDFYAKLN

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=568, Percent_Identity=70.9507042253521, Blast_Score=820, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=540, Percent_Identity=39.2592592592593, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI48994992, Length=500, Percent_Identity=39.4, Blast_Score=342, Evalue=5e-95,
Organism=Escherichia coli, GI1789193, Length=576, Percent_Identity=34.7222222222222, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1787994, Length=453, Percent_Identity=27.5938189845475, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI226510935, Length=210, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 62743; Mature: 62743

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGIAASPGVVFGKALVLKEEPIVLNTQKITADQIEAEKAKFFAGREKAAAQLTAIKEK
CCCCCCCCCCHHCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ARGTLGEEKEAIFEGHLMILEDEELEEEILGYIADNLVTADVAASKVIDMQASMLAEIDD
HCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EYLKERAGDIRDIGNRLLRNILNMHIIDLGDIQEEVILVAYDLTPSETAQLNLDKVLGFI
HHHHHHCCCHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEECCCCCCHHCCHHHHHHHH
TDIGGRTSHTSIMARSLELPAIVGTNDITARVKTGDTLILDAVNNQIHINPSDAALAEFK
HHCCCCCCHHHHHHHHCCCCEEECCCCCEEEEECCCEEEEEECCCEEEECCCHHHHHHHH
AIQERVAAEKAELAKLKDLPAETLDGHRIEVAGNIGTIRDVDGVLRNGGESVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEEE
FMDRSELPSEEEQFQAYKEIVEAMDGKQVVLRTMDIGGDKELPYLNLPKEMNPFLGWRAV
EECHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEECCCHHCCHHHCCEEE
RIGLTRREILDTQLRAVLRASAFGKLAVMFPMIISVEEIRELKAIVAELKEQLRAEGKAF
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DENLQLGIMVETPSAAVNARHLAKEADFFSIGTNDLTQYTLAVDRGNEIIAHLYNPLSPS
CCCCEEEEEEECCCHHHHHHHHHHHCCEEECCCCCCEEEEEEEECCCHHHHHHHCCCCHH
VLNLIKQVIDASHAEGKWTGMCGELAGDVRATALLLGMGLDEFSMSAISVPHVKKLARSI
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHC
NYADAKALADEALAQPTATDIEKLVNDFYAKLN
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITGIAASPGVVFGKALVLKEEPIVLNTQKITADQIEAEKAKFFAGREKAAAQLTAIKEK
CCCCCCCCCCHHCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ARGTLGEEKEAIFEGHLMILEDEELEEEILGYIADNLVTADVAASKVIDMQASMLAEIDD
HCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EYLKERAGDIRDIGNRLLRNILNMHIIDLGDIQEEVILVAYDLTPSETAQLNLDKVLGFI
HHHHHHCCCHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEECCCCCCHHCCHHHHHHHH
TDIGGRTSHTSIMARSLELPAIVGTNDITARVKTGDTLILDAVNNQIHINPSDAALAEFK
HHCCCCCCHHHHHHHHCCCCEEECCCCCEEEEECCCEEEEEECCCEEEECCCHHHHHHHH
AIQERVAAEKAELAKLKDLPAETLDGHRIEVAGNIGTIRDVDGVLRNGGESVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEEE
FMDRSELPSEEEQFQAYKEIVEAMDGKQVVLRTMDIGGDKELPYLNLPKEMNPFLGWRAV
EECHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEECCCHHCCHHHCCEEE
RIGLTRREILDTQLRAVLRASAFGKLAVMFPMIISVEEIRELKAIVAELKEQLRAEGKAF
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DENLQLGIMVETPSAAVNARHLAKEADFFSIGTNDLTQYTLAVDRGNEIIAHLYNPLSPS
CCCCEEEEEEECCCHHHHHHHHHHHCCEEECCCCCCEEEEEEEECCCHHHHHHHCCCCHH
VLNLIKQVIDASHAEGKWTGMCGELAGDVRATALLLGMGLDEFSMSAISVPHVKKLARSI
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHC
NYADAKALADEALAQPTATDIEKLVNDFYAKLN
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]