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The map label for this gene is nudE [H]

Identifier: 126208777

GI number: 126208777

Start: 1504539

End: 1505096

Strand: Reverse

Name: nudE [H]

Synonym: APL_1313

Alternate gene names: 126208777

Gene position: 1505096-1504539 (Counterclockwise)

Preceding gene: 126208780

Following gene: 126208776

Centisome position: 66.17

GC content: 44.09

Gene sequence:

>558_bases
ATGACCACAGTACGTCAGCTCCCGGAAATTCTTTCAGTAGAAACGATCGCTAAAACACGTATCTTTGAAGTACAGGCGGT
CGATTTGCGTTTTTCAAACGGTGAAGAACGTCGATTTGAGCGTTTAACGCCGCAACGTCGTTCTTCCGTGATGGTCATAC
CGATTCAAGATCAGTATCTTATTTTTGTCAAAGAATATGCGGTAGGTTCCGAACGTTATGAGTTAGGTTTTCCGAAAGGG
ATCGTTGATCCGGGCGAAGAGCCGATAGTCAGTGCCAATCGAGAATTACAAGAAGAAATCGGCTTCGGCGCAAAACGGAT
CGAATTTTTACGTTCGCTTTATAGCGGCCCGAGCCATATGTTCGGTTTAATGCACTTATTTATTGCTCAAGATCTTTATC
CGTCCAAATTAGCGGGTGATGAGCCAGAGCCGTTAGAAATCGTACGCATACCGTTAGCCGATATAGACGAATTACTGGCT
GATCCGAATTTTGCGGAATCAAGAAATTTATCCGCTCTGTTTATGCTAAGAGATTATCTGGCAAGCGGTCGGAAATAA

Upstream 100 bases:

>100_bases
ATGATTTTATTTATACGGAATTTACTGCATAAATTTAAATAAATTTTATCATAAAATATGGTATCTTTAGAAAATTAAAT
AGTAGTAATGAGAATATCTT

Downstream 100 bases:

>100_bases
GGGAATTTTAGTATGCAACCATTAAATTCAACGCTGTTGGAAGCGGTTAAACGGATTGCGATAGAAGCGGGAGAGCACCT
TAAACGTTTTTATCAGTGTT

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MTTVRQLPEILSVETIAKTRIFEVQAVDLRFSNGEERRFERLTPQRRSSVMVIPIQDQYLIFVKEYAVGSERYELGFPKG
IVDPGEEPIVSANRELQEEIGFGAKRIEFLRSLYSGPSHMFGLMHLFIAQDLYPSKLAGDEPEPLEIVRIPLADIDELLA
DPNFAESRNLSALFMLRDYLASGRK

Sequences:

>Translated_185_residues
MTTVRQLPEILSVETIAKTRIFEVQAVDLRFSNGEERRFERLTPQRRSSVMVIPIQDQYLIFVKEYAVGSERYELGFPKG
IVDPGEEPIVSANRELQEEIGFGAKRIEFLRSLYSGPSHMFGLMHLFIAQDLYPSKLAGDEPEPLEIVRIPLADIDELLA
DPNFAESRNLSALFMLRDYLASGRK
>Mature_184_residues
TTVRQLPEILSVETIAKTRIFEVQAVDLRFSNGEERRFERLTPQRRSSVMVIPIQDQYLIFVKEYAVGSERYELGFPKGI
VDPGEEPIVSANRELQEEIGFGAKRIEFLRSLYSGPSHMFGLMHLFIAQDLYPSKLAGDEPEPLEIVRIPLADIDELLAD
PNFAESRNLSALFMLRDYLASGRK

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=185, Percent_Identity=54.0540540540541, Blast_Score=210, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 21121; Mature: 20989

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVRQLPEILSVETIAKTRIFEVQAVDLRFSNGEERRFERLTPQRRSSVMVIPIQDQYL
CCCHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHHCCHHHHCCEEEEEECCCEE
IFVKEYAVGSERYELGFPKGIVDPGEEPIVSANRELQEEIGFGAKRIEFLRSLYSGPSHM
EEEEEHHCCCCCEECCCCCCCCCCCCCCCHHCCHHHHHHHCCCHHHHHHHHHHHCCHHHH
FGLMHLFIAQDLYPSKLAGDEPEPLEIVRIPLADIDELLADPNFAESRNLSALFMLRDYL
HHHHHHHHHHCCCCHHHCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
ASGRK
HCCCC
>Mature Secondary Structure 
TTVRQLPEILSVETIAKTRIFEVQAVDLRFSNGEERRFERLTPQRRSSVMVIPIQDQYL
CCHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHHCCHHHHCCEEEEEECCCEE
IFVKEYAVGSERYELGFPKGIVDPGEEPIVSANRELQEEIGFGAKRIEFLRSLYSGPSHM
EEEEEHHCCCCCEECCCCCCCCCCCCCCCHHCCHHHHHHHCCCHHHHHHHHHHHCCHHHH
FGLMHLFIAQDLYPSKLAGDEPEPLEIVRIPLADIDELLADPNFAESRNLSALFMLRDYL
HHHHHHHHHHCCCCHHHCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
ASGRK
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]