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The map label for this gene is mdh

Identifier: 126208762

GI number: 126208762

Start: 1485137

End: 1486090

Strand: Direct

Name: mdh

Synonym: APL_1296

Alternate gene names: 126208762

Gene position: 1485137-1486090 (Clockwise)

Preceding gene: 126208759

Following gene: 126208763

Centisome position: 65.3

GC content: 46.02

Gene sequence:

>954_bases
ATGAAAGTTGCACTTTTAGGCGCAGCGGGCGGGATTGGTCAAACCTTAGCCCTATTATTAAAACTTCGTTTACCTGTCGG
CACCGACTTAGCGTTATATGATATTTCCCCCGTAACGCCGGGGATTGCGGTCGATATCAGCCACATACCGACTTCGGTTA
GTGCGGTAGGTTACTCCGGTGAAGATCCGAGTGAGGCGTTAAAAGGCGCAAATCTTGTGATTATTACCGCCGGCGTAGCG
CGTAAACCGGGTATGACGCGTGCGGATTTATTCAATATCAATGCGGATATCGTTAAAAATCTAGTCGAAAAAGTAGCGGA
AGTTTGCCCGAAAGCTTGTATCGGTATCGTAACCAATCCGGTCAATACCCTTGTGCCGATTGCGGCGGAAGTACTACGTA
AAGCCGGTGTTTATGATAAACGTAAATTATTCGGTGTAACGACGCTTGACGTGGTTCGCGCCAAAACATTTACTTCCGAA
TTAAAAGAGAAACATGTCGAAACGGTTAAAGTTCCGGTAATCGGCGGTCACTCAGGTCCGACTATCCTTCCTCTTCTTTC
TCAAGCATTGTCCGAAGGTCTTCCGCTTTCATTTACTCAAGAAGAAATCGAGGCTTTAACTTATCGTATCCAAAATGCCG
GTACTGAAGTAGTAGAAGCTAAAGCCGGCGGCGGGTCGGCAACGCTTTCTATGGCGGAATCCGGCGCACGTTTTGCCGTT
GCGGTATTTAAAGCGTTATTAGGTGAAGATTGCGTACGTTACGCCTATGTAGAAAGTAAAGAGGGTTCTGGTTATCCCGA
ATTCTTTGCTCATCCGGTACGTTTCGGATTAACCGGTGTGGAAGAACTGTTACCTATCGGCAAATTAAGCGAATATGAAC
AAGCAAAACTGGGCGAGTTAAAACCTGTGCTTGAAGCGGATATTGCTCTCGGTAAAAATTTTGTAAATCCATAA

Upstream 100 bases:

>100_bases
ATTTTTTATAAATATTAGTTTATTAAGCATCAATCCGAATAAATGTTTGGTAAACTTACCCTAGTTCTATTTTTTAACTA
TTATTGATTAGGAGTTATTT

Downstream 100 bases:

>100_bases
TGAAATATAAATAAAGGGTTGAGTTATTCAACCCTTTTTTATATGCTCAAAGCGGTTGCTTATTTTTTAACTCTATAAGG
ATTTTAATTATGCGTAATCG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKVALLGAAGGIGQTLALLLKLRLPVGTDLALYDISPVTPGIAVDISHIPTSVSAVGYSGEDPSEALKGANLVIITAGVA
RKPGMTRADLFNINADIVKNLVEKVAEVCPKACIGIVTNPVNTLVPIAAEVLRKAGVYDKRKLFGVTTLDVVRAKTFTSE
LKEKHVETVKVPVIGGHSGPTILPLLSQALSEGLPLSFTQEEIEALTYRIQNAGTEVVEAKAGGGSATLSMAESGARFAV
AVFKALLGEDCVRYAYVESKEGSGYPEFFAHPVRFGLTGVEELLPIGKLSEYEQAKLGELKPVLEADIALGKNFVNP

Sequences:

>Translated_317_residues
MKVALLGAAGGIGQTLALLLKLRLPVGTDLALYDISPVTPGIAVDISHIPTSVSAVGYSGEDPSEALKGANLVIITAGVA
RKPGMTRADLFNINADIVKNLVEKVAEVCPKACIGIVTNPVNTLVPIAAEVLRKAGVYDKRKLFGVTTLDVVRAKTFTSE
LKEKHVETVKVPVIGGHSGPTILPLLSQALSEGLPLSFTQEEIEALTYRIQNAGTEVVEAKAGGGSATLSMAESGARFAV
AVFKALLGEDCVRYAYVESKEGSGYPEFFAHPVRFGLTGVEELLPIGKLSEYEQAKLGELKPVLEADIALGKNFVNP
>Mature_317_residues
MKVALLGAAGGIGQTLALLLKLRLPVGTDLALYDISPVTPGIAVDISHIPTSVSAVGYSGEDPSEALKGANLVIITAGVA
RKPGMTRADLFNINADIVKNLVEKVAEVCPKACIGIVTNPVNTLVPIAAEVLRKAGVYDKRKLFGVTTLDVVRAKTFTSE
LKEKHVETVKVPVIGGHSGPTILPLLSQALSEGLPLSFTQEEIEALTYRIQNAGTEVVEAKAGGGSATLSMAESGARFAV
AVFKALLGEDCVRYAYVESKEGSGYPEFFAHPVRFGLTGVEELLPIGKLSEYEQAKLGELKPVLEADIALGKNFVNP

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family

Homologues:

Organism=Homo sapiens, GI21735621, Length=316, Percent_Identity=51.5822784810127, Blast_Score=291, Evalue=6e-79,
Organism=Homo sapiens, GI47059044, Length=315, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI221136809, Length=315, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1789632, Length=319, Percent_Identity=64.8902821316615, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17554310, Length=317, Percent_Identity=54.5741324921136, Blast_Score=305, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6322765, Length=322, Percent_Identity=49.3788819875776, Blast_Score=261, Evalue=8e-71,
Organism=Saccharomyces cerevisiae, GI6320125, Length=343, Percent_Identity=40.2332361516035, Blast_Score=195, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6324446, Length=324, Percent_Identity=39.5061728395062, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24647881, Length=319, Percent_Identity=53.9184952978056, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24663599, Length=315, Percent_Identity=46.031746031746, Blast_Score=248, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24663595, Length=304, Percent_Identity=43.421052631579, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_ACTP2 (A3N1U6)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053987.1
- ProteinModelPortal:   A3N1U6
- SMR:   A3N1U6
- STRING:   A3N1U6
- GeneID:   4850462
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1296
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   FVRSEET
- PhylomeDB:   A3N1U6
- ProtClustDB:   PRK05086
- BioCyc:   APLE416269:APL_1296-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01516
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010097
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11540:SF1
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01772

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33347; Mature: 33347

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 177-177 BINDING 34-34 BINDING 81-81 BINDING 87-87 BINDING 94-94 BINDING 119-119 BINDING 153-153 BINDING 231-231

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVALLGAAGGIGQTLALLLKLRLPVGTDLALYDISPVTPGIAVDISHIPTSVSAVGYSG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCHHCCCCCC
EDPSEALKGANLVIITAGVARKPGMTRADLFNINADIVKNLVEKVAEVCPKACIGIVTNP
CCHHHHHCCCCEEEEECCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHCHHHHHHHHCCC
VNTLVPIAAEVLRKAGVYDKRKLFGVTTLDVVRAKTFTSELKEKHVETVKVPVIGGHSGP
HHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCC
TILPLLSQALSEGLPLSFTQEEIEALTYRIQNAGTEVVEAKAGGGSATLSMAESGARFAV
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHHHHCCCHHHH
AVFKALLGEDCVRYAYVESKEGSGYPEFFAHPVRFGLTGVEELLPIGKLSEYEQAKLGEL
HHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHCCC
KPVLEADIALGKNFVNP
CHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVALLGAAGGIGQTLALLLKLRLPVGTDLALYDISPVTPGIAVDISHIPTSVSAVGYSG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCHHCCCCCC
EDPSEALKGANLVIITAGVARKPGMTRADLFNINADIVKNLVEKVAEVCPKACIGIVTNP
CCHHHHHCCCCEEEEECCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHCHHHHHHHHCCC
VNTLVPIAAEVLRKAGVYDKRKLFGVTTLDVVRAKTFTSELKEKHVETVKVPVIGGHSGP
HHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCC
TILPLLSQALSEGLPLSFTQEEIEALTYRIQNAGTEVVEAKAGGGSATLSMAESGARFAV
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHHHHCCCHHHH
AVFKALLGEDCVRYAYVESKEGSGYPEFFAHPVRFGLTGVEELLPIGKLSEYEQAKLGEL
HHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHCCC
KPVLEADIALGKNFVNP
CHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA