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The map label for this gene is pgk

Identifier: 126208717

GI number: 126208717

Start: 1437463

End: 1438638

Strand: Reverse

Name: pgk

Synonym: APL_1251

Alternate gene names: 126208717

Gene position: 1438638-1437463 (Counterclockwise)

Preceding gene: 126208718

Following gene: 126208716

Centisome position: 63.25

GC content: 45.32

Gene sequence:

>1176_bases
ATGTCAGTGATTAAAATGGCAGACCTTGATCTTGCCGGTAAACGTTTATTTATCCGTGCCGATTTAAACGTACCGGTAAA
AGATGGCAAAGTGACATCTGACGCACGTATTCGTGCAACCATTCCTACCTTAAAATTAGCTTTACAAAAAGGTGCTAAAG
TAATGGTGACTTCTCACTTAGGTCGTCCTACTGAAGGTGTATTCGAAGAAGCAAACTCTTTACAACCTGTGGTTGATTAC
TTAAACGCATCGGATTTAGGCGTACCTGTACGTTTAGTACGCGATTACCTAGACGGCGTTGAAGTTGCGGAAAACGAAAT
CGTAGTACTTGAAAACGTTCGTATCAACAAAGGCGAAAAGAAAAACGATCCTGAACTTGCGAAAAAATATGCGGCGCTTT
GTGACGTATTCGTGATGGATGCATTCGGTACGGCACACCGTGCGGAAGGTTCGACTTACGGTGTTGCGGAATACGCGCCG
GTTGCTTGTGCGGGTCCTCTATTAGCGGCGGAATTGGATGCGCTAGGTAAAGCCTTAAAAGAACCGCAACGTCCGATGTT
AGCGATCGTAGGCGGCTCAAAAGTTTCTACTAAATTAACCGTATTAGACAGCCTTTCAAAAATTGCAGACCAATTAATCG
TTGGCGGCGGTATCGCAAATACTTTCATTGCTGCGGAAGGTCACCCGGTAGGCAAATCGTTATACGAAGCGGATTTAATT
CCTGAAGCGAAACGTTTAGCGGCGGCAACCAATATTCCTGTTCCGGTTGATGTACGCGTCGGTACCGAATTCTCTGAAAT
CGCACCGGCAACGGAAAAAGCGGTATCTGAAGTTCAAGCGGACGAATCAATTTTCGATATCGGTGACAAATCTGCAGAAG
AATTGGCAAATATCATCAAATCGGCAAAAACCATTCTTTGGAACGGTCCGGTCGGTGTATTTGAATTCCCTAACTTCCGT
AAAGGTACCGAAGTGATTTCAAACGCTATCGCAGAAGCGACCGCAAACGGCGCATTCTCGATCGCAGGTGGCGGTGATAC
CTTAGCGGCAATCGATTTATTCGGTATTGCGGATAAAATTTCTTATATCTCAACCGGCGGCGGCGCCTTCTTAGAATTCG
TAGAAGGTAAAGTATTACCGGCGGTCGAAATTTTAGAAAAACGTGCAAACGGCTAA

Upstream 100 bases:

>100_bases
CAAAATTAAGCAAGAATTTGATCTACATTCGCCCAAAATAGGTAGAAAATGATATAATTGCCCTGTTTTATTTTTGAAAC
TTAAAAAAGAGGAACTTACT

Downstream 100 bases:

>100_bases
TTGATCTTGTGTAGGGGCGTATTGCATACGTCCTAAAACTCTCCATTAAGGGCGTATGTAATACGCCCCTACTGAATCAT
TTATTTAAAGGAAACTCAAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSVIKMADLDLAGKRLFIRADLNVPVKDGKVTSDARIRATIPTLKLALQKGAKVMVTSHLGRPTEGVFEEANSLQPVVDY
LNASDLGVPVRLVRDYLDGVEVAENEIVVLENVRINKGEKKNDPELAKKYAALCDVFVMDAFGTAHRAEGSTYGVAEYAP
VACAGPLLAAELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAEGHPVGKSLYEADLI
PEAKRLAAATNIPVPVDVRVGTEFSEIAPATEKAVSEVQADESIFDIGDKSAEELANIIKSAKTILWNGPVGVFEFPNFR
KGTEVISNAIAEATANGAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVEILEKRANG

Sequences:

>Translated_391_residues
MSVIKMADLDLAGKRLFIRADLNVPVKDGKVTSDARIRATIPTLKLALQKGAKVMVTSHLGRPTEGVFEEANSLQPVVDY
LNASDLGVPVRLVRDYLDGVEVAENEIVVLENVRINKGEKKNDPELAKKYAALCDVFVMDAFGTAHRAEGSTYGVAEYAP
VACAGPLLAAELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAEGHPVGKSLYEADLI
PEAKRLAAATNIPVPVDVRVGTEFSEIAPATEKAVSEVQADESIFDIGDKSAEELANIIKSAKTILWNGPVGVFEFPNFR
KGTEVISNAIAEATANGAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVEILEKRANG
>Mature_390_residues
SVIKMADLDLAGKRLFIRADLNVPVKDGKVTSDARIRATIPTLKLALQKGAKVMVTSHLGRPTEGVFEEANSLQPVVDYL
NASDLGVPVRLVRDYLDGVEVAENEIVVLENVRINKGEKKNDPELAKKYAALCDVFVMDAFGTAHRAEGSTYGVAEYAPV
ACAGPLLAAELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAEGHPVGKSLYEADLIP
EAKRLAAATNIPVPVDVRVGTEFSEIAPATEKAVSEVQADESIFDIGDKSAEELANIIKSAKTILWNGPVGVFEFPNFRK
GTEVISNAIAEATANGAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVEILEKRANG

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=409, Percent_Identity=37.8973105134474, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI31543397, Length=409, Percent_Identity=38.1418092909535, Blast_Score=245, Evalue=6e-65,
Organism=Escherichia coli, GI1789294, Length=389, Percent_Identity=80.719794344473, Blast_Score=627, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17508823, Length=414, Percent_Identity=42.0289855072464, Blast_Score=262, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6319857, Length=417, Percent_Identity=39.3285371702638, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI17136394, Length=414, Percent_Identity=40.0966183574879, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24581203, Length=414, Percent_Identity=38.8888888888889, Blast_Score=244, Evalue=5e-65,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_ACTP2 (A3N1Q1)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053942.1
- ProteinModelPortal:   A3N1Q1
- SMR:   A3N1Q1
- STRING:   A3N1Q1
- GeneID:   4849213
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1251
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   STYGIAQ
- PhylomeDB:   A3N1Q1
- ProtClustDB:   PRK00073
- BioCyc:   APLE416269:APL_1251-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41171; Mature: 41039

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 114-114 BINDING 147-147 BINDING 198-198 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIKMADLDLAGKRLFIRADLNVPVKDGKVTSDARIRATIPTLKLALQKGAKVMVTSHL
CCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCEEEEEECC
GRPTEGVFEEANSLQPVVDYLNASDLGVPVRLVRDYLDGVEVAENEIVVLENVRINKGEK
CCCCHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHCCCEECCCCEEEEEEEEECCCCC
KNDPELAKKYAALCDVFVMDAFGTAHRAEGSTYGVAEYAPVACAGPLLAAELDALGKALK
CCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHCCCHHHCCHHHHHHHHHHHHHHC
EPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAEGHPVGKSLYEADLI
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCHHHHEEEECCCCCCHHHHHHHCC
PEAKRLAAATNIPVPVDVRVGTEFSEIAPATEKAVSEVQADESIFDIGDKSAEELANIIK
CHHHHHHHHCCCCCEEEEEECCCHHHHCCHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHH
SAKTILWNGPVGVFEFPNFRKGTEVISNAIAEATANGAFSIAGGGDTLAAIDLFGIADKI
HHCEEEECCCCCCEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHH
SYISTGGGAFLEFVEGKVLPAVEILEKRANG
HHEECCCHHHHHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SVIKMADLDLAGKRLFIRADLNVPVKDGKVTSDARIRATIPTLKLALQKGAKVMVTSHL
CEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCEEEEEECC
GRPTEGVFEEANSLQPVVDYLNASDLGVPVRLVRDYLDGVEVAENEIVVLENVRINKGEK
CCCCHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHCCCEECCCCEEEEEEEEECCCCC
KNDPELAKKYAALCDVFVMDAFGTAHRAEGSTYGVAEYAPVACAGPLLAAELDALGKALK
CCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHCCCHHHCCHHHHHHHHHHHHHHC
EPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAEGHPVGKSLYEADLI
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCHHHHEEEECCCCCCHHHHHHHCC
PEAKRLAAATNIPVPVDVRVGTEFSEIAPATEKAVSEVQADESIFDIGDKSAEELANIIK
CHHHHHHHHCCCCCEEEEEECCCHHHHCCHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHH
SAKTILWNGPVGVFEFPNFRKGTEVISNAIAEATANGAFSIAGGGDTLAAIDLFGIADKI
HHCEEEECCCCCCEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHH
SYISTGGGAFLEFVEGKVLPAVEILEKRANG
HHEECCCHHHHHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA