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The map label for this gene is priB

Identifier: 126208638

GI number: 126208638

Start: 1346539

End: 1346913

Strand: Reverse

Name: priB

Synonym: APL_1170

Alternate gene names: 126208638

Gene position: 1346913-1346539 (Counterclockwise)

Preceding gene: 126208639

Following gene: 126208637

Centisome position: 59.22

GC content: 39.47

Gene sequence:

>375_bases
TTGAATCAGAGGAAGCTGGCGAGTAATTCGCCGATTGATAATTGCTTAATTCTGAGCGGCTCGGTCGCTTCAACGGTTAA
GCAAAGCCAAAACCCGTTAGGGGTTCCGAATTATCGTTTTTGGCTTGAACATCGTTCAATTCAAACCGAAGTAAATTTAG
AGCGTCAAGCGTGGTGTAAAATCCAAGTGATTTTAAATGGCAATCAATTTAGTTTAATAACCCAACAAATAAAGCTCGGC
GATAAAATTAGAGTTTACGGATTTATCCACACTCACAAAGACTATAACGGTTTAAGCCAATTAGTCGTTCATGCCGAACA
TATTGAATTTATAGATCAGGAGAAGCCAAATGGCACGTTATTTCCGTCGTCGTAA

Upstream 100 bases:

>100_bases
GTTCTTCGTAACTTAATCGTTCACACTAAAGCGGCAGTAACTGAAGCGTCACCAATGGTGAAAGCGAAAGAAAGCAAAGT
TGCTGAAGCGGTAGCTGAAG

Downstream 100 bases:

>100_bases
GTTCTGCCGTTTCACAGCGGAAAATGTTGTTGAGATCGATTACAAAGATATCGCTACATTAAAGAACTACATTTCTGAAA
GCGGCAAGATTGTTCCTAGC

Product: primosomal replication protein N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MNQRKLASNSPIDNCLILSGSVASTVKQSQNPLGVPNYRFWLEHRSIQTEVNLERQAWCKIQVILNGNQFSLITQQIKLG
DKIRVYGFIHTHKDYNGLSQLVVHAEHIEFIDQEKPNGTLFPSS

Sequences:

>Translated_124_residues
MNQRKLASNSPIDNCLILSGSVASTVKQSQNPLGVPNYRFWLEHRSIQTEVNLERQAWCKIQVILNGNQFSLITQQIKLG
DKIRVYGFIHTHKDYNGLSQLVVHAEHIEFIDQEKPNGTLFPSS
>Mature_124_residues
MNQRKLASNSPIDNCLILSGSVASTVKQSQNPLGVPNYRFWLEHRSIQTEVNLERQAWCKIQVILNGNQFSLITQQIKLG
DKIRVYGFIHTHKDYNGLSQLVVHAEHIEFIDQEKPNGTLFPSS

Specific function: Binds single-stranded DNA at the primosome assembly site (PAS)

COG id: COG2965

COG function: function code L; Primosomal replication protein N

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain

Homologues:

Organism=Escherichia coli, GI1790645, Length=101, Percent_Identity=47.5247524752475, Blast_Score=103, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIB_ACTP2 (A3N1H2)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053863.1
- ProteinModelPortal:   A3N1H2
- SMR:   A3N1H2
- STRING:   A3N1H2
- GeneID:   4849208
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1170
- eggNOG:   COG2965
- HOGENOM:   HBG632331
- OMA:   WCRMPVI
- PhylomeDB:   A3N1H2
- ProtClustDB:   PRK02801
- BioCyc:   APLE416269:APL_1170-MONOMER
- HAMAP:   MF_00720
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF00436 SSB; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 14131; Mature: 14131

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQRKLASNSPIDNCLILSGSVASTVKQSQNPLGVPNYRFWLEHRSIQTEVNLERQAWCK
CCCCCCCCCCCCCEEEEEECCHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCCCCEEE
IQVILNGNQFSLITQQIKLGDKIRVYGFIHTHKDYNGLSQLVVHAEHIEFIDQEKPNGTL
EEEEEECCEEEEEEEEHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCC
FPSS
CCCC
>Mature Secondary Structure
MNQRKLASNSPIDNCLILSGSVASTVKQSQNPLGVPNYRFWLEHRSIQTEVNLERQAWCK
CCCCCCCCCCCCCEEEEEECCHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCCCCEEE
IQVILNGNQFSLITQQIKLGDKIRVYGFIHTHKDYNGLSQLVVHAEHIEFIDQEKPNGTL
EEEEEECCEEEEEEEEHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCC
FPSS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA