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The map label for this gene is metR [H]

Identifier: 126208626

GI number: 126208626

Start: 1334889

End: 1335818

Strand: Direct

Name: metR [H]

Synonym: APL_1158

Alternate gene names: 126208626

Gene position: 1334889-1335818 (Clockwise)

Preceding gene: 126208623

Following gene: 126208631

Centisome position: 58.69

GC content: 44.3

Gene sequence:

>930_bases
ATGAAACCGATTTTTCTTGAACTACGCCACTTAAAAACACTGGTCGCTTTAAAAGAAAGCGGCAGCGTTTCTCTTGCGGC
AAAACGCGTACATCTCACGCAATCCGCGCTCTCTCATCAGCTTAAATTATTGGAAGATCAATATGAACTGACCTTATTTG
AGCGTAAAACCCAGCCGCTACGCTTTACGCCCGCCGGAGATCGGCTGGTGAAGCTGGCGCATGATATCTTGCCTAAGATT
GTAGAAGCCGAACTCGATCTTGCCCGTGTTAAACAAGGGGAAGCGGGAGAATTACGTATTGCGGTCGAATGTCATACTTG
CTTTGACTGGCTGATGCCGGCAATGGATACTTTCCGCCAAAGTTGGCCTTTAGTGGAATTGGATATTGTATCCGGTTTCC
ATACCGATACTATCGGGTTACTCTTAACGCATCGTGCGGATTGGGCGGTTGTTGCCGAAGCGGAAGAAACGGAAGGCATT
ATTTATCGTCCGTTATTTTCTTATGAAATGGTTGGCATTTGTGCTAAGGATCATCCTTTAGCCGATAAAGATATTTGGCA
AGCGGAAGATTTTATTGACGAAACTTGGATTACTTATCCGGTACCGGACGATATGTTGGATTTACTGCGTAAAGTGTTGC
GCCCTGCGGGGATTATGCCTACACGCAGAACCAGCGAATTAACCATTGCGATTATTCAGTTAGTTGCGAGTAAAAGAGGG
ATTGCAACCTTACCGTATTGGGCGGTGAAACCTTATTTGGATCGAGGCTATGTGGTTGCCAAGAAAATTACTCAACAAGG
GCTATACAGTAATCTTTACGGAGCATTCAGAGAATCGGATCAACATTACGCTTATCTTAATGATTTCCATAATACGGTTA
AATCGCAAAGTTTTGAAACGCTACCGGGACTGATGGTATTGGATAAATAA

Upstream 100 bases:

>100_bases
AAACGCCGAACCCATGATTATTTTCATTTTTAGTCTTGGCATACCAAGCATTTATCTATAGTATCTTTCATCATCTTTCT
TTTTATAATGAACTATTTAC

Downstream 100 bases:

>100_bases
CTTTGAATAAAATTAAAAGCGGTAGAATTTGTTAAAATTTATACAAAATCTACCGCTTTGCGTATTAAATCAATATGTTA
CTTTTGTTGTTTTGCGCCAA

Product: HTH-type transcriptional regulator MetR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKPIFLELRHLKTLVALKESGSVSLAAKRVHLTQSALSHQLKLLEDQYELTLFERKTQPLRFTPAGDRLVKLAHDILPKI
VEAELDLARVKQGEAGELRIAVECHTCFDWLMPAMDTFRQSWPLVELDIVSGFHTDTIGLLLTHRADWAVVAEAEETEGI
IYRPLFSYEMVGICAKDHPLADKDIWQAEDFIDETWITYPVPDDMLDLLRKVLRPAGIMPTRRTSELTIAIIQLVASKRG
IATLPYWAVKPYLDRGYVVAKKITQQGLYSNLYGAFRESDQHYAYLNDFHNTVKSQSFETLPGLMVLDK

Sequences:

>Translated_309_residues
MKPIFLELRHLKTLVALKESGSVSLAAKRVHLTQSALSHQLKLLEDQYELTLFERKTQPLRFTPAGDRLVKLAHDILPKI
VEAELDLARVKQGEAGELRIAVECHTCFDWLMPAMDTFRQSWPLVELDIVSGFHTDTIGLLLTHRADWAVVAEAEETEGI
IYRPLFSYEMVGICAKDHPLADKDIWQAEDFIDETWITYPVPDDMLDLLRKVLRPAGIMPTRRTSELTIAIIQLVASKRG
IATLPYWAVKPYLDRGYVVAKKITQQGLYSNLYGAFRESDQHYAYLNDFHNTVKSQSFETLPGLMVLDK
>Mature_309_residues
MKPIFLELRHLKTLVALKESGSVSLAAKRVHLTQSALSHQLKLLEDQYELTLFERKTQPLRFTPAGDRLVKLAHDILPKI
VEAELDLARVKQGEAGELRIAVECHTCFDWLMPAMDTFRQSWPLVELDIVSGFHTDTIGLLLTHRADWAVVAEAEETEGI
IYRPLFSYEMVGICAKDHPLADKDIWQAEDFIDETWITYPVPDDMLDLLRKVLRPAGIMPTRRTSELTIAIIQLVASKRG
IATLPYWAVKPYLDRGYVVAKKITQQGLYSNLYGAFRESDQHYAYLNDFHNTVKSQSFETLPGLMVLDK

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=298, Percent_Identity=40.6040268456376, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI145693105, Length=183, Percent_Identity=28.9617486338798, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787879, Length=248, Percent_Identity=27.4193548387097, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786401, Length=169, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790399, Length=260, Percent_Identity=29.6153846153846, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787530, Length=296, Percent_Identity=24.6621621621622, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082024, Length=292, Percent_Identity=25, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788297, Length=255, Percent_Identity=28.2352941176471, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790208, Length=195, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI157672245, Length=176, Percent_Identity=27.8409090909091, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789204, Length=209, Percent_Identity=28.7081339712919, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35236; Mature: 35236

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIFLELRHLKTLVALKESGSVSLAAKRVHLTQSALSHQLKLLEDQYELTLFERKTQPL
CCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCE
RFTPAGDRLVKLAHDILPKIVEAELDLARVKQGEAGELRIAVECHTCFDWLMPAMDTFRQ
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
SWPLVELDIVSGFHTDTIGLLLTHRADWAVVAEAEETEGIIYRPLFSYEMVGICAKDHPL
CCCEEEEEECCCCCCCHHEEEEEECCCEEEEEECCCCCCEEEECHHHHHEEEEEECCCCC
ADKDIWQAEDFIDETWITYPVPDDMLDLLRKVLRPAGIMPTRRTSELTIAIIQLVASKRG
CCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
IATLPYWAVKPYLDRGYVVAKKITQQGLYSNLYGAFRESDQHYAYLNDFHNTVKSQSFET
CEECCHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHC
LPGLMVLDK
CCCEEEECC
>Mature Secondary Structure
MKPIFLELRHLKTLVALKESGSVSLAAKRVHLTQSALSHQLKLLEDQYELTLFERKTQPL
CCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCE
RFTPAGDRLVKLAHDILPKIVEAELDLARVKQGEAGELRIAVECHTCFDWLMPAMDTFRQ
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
SWPLVELDIVSGFHTDTIGLLLTHRADWAVVAEAEETEGIIYRPLFSYEMVGICAKDHPL
CCCEEEEEECCCCCCCHHEEEEEECCCEEEEEECCCCCCEEEECHHHHHEEEEEECCCCC
ADKDIWQAEDFIDETWITYPVPDDMLDLLRKVLRPAGIMPTRRTSELTIAIIQLVASKRG
CCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
IATLPYWAVKPYLDRGYVVAKKITQQGLYSNLYGAFRESDQHYAYLNDFHNTVKSQSFET
CEECCHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHC
LPGLMVLDK
CCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]