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The map label for this gene is ruvC

Identifier: 126208623

GI number: 126208623

Start: 1328410

End: 1328988

Strand: Direct

Name: ruvC

Synonym: APL_1153

Alternate gene names: 126208623

Gene position: 1328410-1328988 (Clockwise)

Preceding gene: 126208622

Following gene: 126208626

Centisome position: 58.4

GC content: 49.05

Gene sequence:

>579_bases
ATGCCGATTATTTTAGGAATTGACCCCGGTTCACGGGTTACCGGTTATGGCGTAATTCGCCAAACCGGACGCCATTTAGA
ATATTTAGGAAGCGGTGCTATTCGCACTTCCGTCGATGATTTACCGACTCGCTTAAAACGCATTTATGCGGGCGTAACCG
AAATCATCACTCAATTCCACCCCGATATGTTTGCAATTGAACAGGTGTTTATGGCGAAAAACGCCGATTCCGCCTTAAAA
CTCGGGCAAGCTCGCGGTACGGCGATTGTCGCGGCGGTAAATCACGACTTACCGGTGTTTGAATATGCCGCCCGTTTAGT
GAAACAAACCGTCACCGGTATCGGCTCGGCGGATAAAATCCAAGTGCAGGATATGGTCACTCGAATGCTTCAACTTTCTT
CCAAACCGCAAGCCGATGCCGCCGATGCGCTTGCGATTGCGATTACGCACGCTCACTCGATCCAACATTCCTTAATCGTT
GCGAAGCAGAGCGACCAAAAAGTCGGTAGCGACAAAGAACAAATCCTCGCTTTGATGAAAACTCGTTACAGTCGGGGACG
ATTCCGTTTAAAAGGGTAA

Upstream 100 bases:

>100_bases
CAAAATTAAATAAATAACAGATTGATTCGTTTAGATAAGCGGGCGGATTTGTAAAATTTTTTGCAAATCCGCCCGCTTGT
TTTCATTCATTGGACAAGCT

Downstream 100 bases:

>100_bases
AAATAAAGAGTAATCAATCGCTTGATTACTCTTTCCGCTCTAAGGATTTTTTTCGGTAGCCGGCTGAGTCGCTTCCGTTT
TTTCTACTTCTGCTTTTTCC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MPIILGIDPGSRVTGYGVIRQTGRHLEYLGSGAIRTSVDDLPTRLKRIYAGVTEIITQFHPDMFAIEQVFMAKNADSALK
LGQARGTAIVAAVNHDLPVFEYAARLVKQTVTGIGSADKIQVQDMVTRMLQLSSKPQADAADALAIAITHAHSIQHSLIV
AKQSDQKVGSDKEQILALMKTRYSRGRFRLKG

Sequences:

>Translated_192_residues
MPIILGIDPGSRVTGYGVIRQTGRHLEYLGSGAIRTSVDDLPTRLKRIYAGVTEIITQFHPDMFAIEQVFMAKNADSALK
LGQARGTAIVAAVNHDLPVFEYAARLVKQTVTGIGSADKIQVQDMVTRMLQLSSKPQADAADALAIAITHAHSIQHSLIV
AKQSDQKVGSDKEQILALMKTRYSRGRFRLKG
>Mature_191_residues
PIILGIDPGSRVTGYGVIRQTGRHLEYLGSGAIRTSVDDLPTRLKRIYAGVTEIITQFHPDMFAIEQVFMAKNADSALKL
GQARGTAIVAAVNHDLPVFEYAARLVKQTVTGIGSADKIQVQDMVTRMLQLSSKPQADAADALAIAITHAHSIQHSLIVA
KQSDQKVGSDKEQILALMKTRYSRGRFRLKG

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=158, Percent_Identity=80.379746835443, Blast_Score=235, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_ACTP2 (A3N1F7)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053848.1
- ProteinModelPortal:   A3N1F7
- SMR:   A3N1F7
- STRING:   A3N1F7
- GeneID:   4848696
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1153
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- PhylomeDB:   A3N1F7
- ProtClustDB:   PRK00039
- BioCyc:   APLE416269:APL_1153-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 20924; Mature: 20792

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIILGIDPGSRVTGYGVIRQTGRHLEYLGSGAIRTSVDDLPTRLKRIYAGVTEIITQFH
CCEEEECCCCCCCHHHHHHHHHCHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHC
PDMFAIEQVFMAKNADSALKLGQARGTAIVAAVNHDLPVFEYAARLVKQTVTGIGSADKI
CCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEE
QVQDMVTRMLQLSSKPQADAADALAIAITHAHSIQHSLIVAKQSDQKVGSDKEQILALMK
EHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHH
TRYSRGRFRLKG
HHHCCCCEEECC
>Mature Secondary Structure 
PIILGIDPGSRVTGYGVIRQTGRHLEYLGSGAIRTSVDDLPTRLKRIYAGVTEIITQFH
CEEEECCCCCCCHHHHHHHHHCHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHC
PDMFAIEQVFMAKNADSALKLGQARGTAIVAAVNHDLPVFEYAARLVKQTVTGIGSADKI
CCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEE
QVQDMVTRMLQLSSKPQADAADALAIAITHAHSIQHSLIVAKQSDQKVGSDKEQILALMK
EHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHH
TRYSRGRFRLKG
HHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA