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The map label for this gene is aspS

Identifier: 126208619

GI number: 126208619

Start: 1324625

End: 1326400

Strand: Direct

Name: aspS

Synonym: APL_1149

Alternate gene names: 126208619

Gene position: 1324625-1326400 (Clockwise)

Preceding gene: 126208618

Following gene: 126208620

Centisome position: 58.24

GC content: 45.1

Gene sequence:

>1776_bases
ATGATGCGTTCTCATTACTGCGGTGCTTTAAACCGCTCGCACGTTGGTCAAACCGTAACATTAAGCGGTTGGGTTCACCG
TGTGCGTAACCTCGGTCGTTTTATTTTTATGCAAATCCGTGACCGTGAAGGTATCGTGCAAGTTTTCTTTGACGAGAAAG
ACGAAGCGATTTTCAAAATCGCTTCAAGCCTACGTTCGGAAGCCTGTGTGCAAATTCAAGGTGAAGTGATTGCCCGTGAC
GAATCGCAAATCAACAAAGAGATGGCGACCGGTGAAATCGAAGTATTAGTGAAAAACGTCGTTGTGTACAACAATGCCGA
CGTTTTACCGCTTGATTTCAACCAAAACAATACTGAAGAACAACGTTTAAAATATCGCTATCTTGACTTACGCCGTCCGG
AAATGGCGGAAAAACTCAAAACACGTGCGAAAATCACCAGCTTCGTACGCCGCTATATGGACGACAACGGTTTCCTTGAT
ATCGAGACGCCAATGCTAACCAAAGCGACACCGGAAGGCGCACGTGACTATTTAGTACCAAGCCGTGTACACAACGGTAA
ATTCTATGCCTTACCGCAATCACCGCAGCTTTTCAAACAGTTGCTAATGATGTCGGGTTTTGACCGTTACTACCAAATCG
TAAAATGTTTCCGTGATGAAGATTTACGTGCCGATCGTCAGCCGGAATTTACCCAAATCGATGTGGAAACCTCATTCTTA
ACCGCTGAAGAAGTGCGTGAATTAATGGAAAATATGATTCACGGCTTATGGTTGGATCGCTTAAACGTAGATTTAGGCAA
ATTCCCAATCATGACGTGGCAGGAAGCGATGCAACGTTTCGGTTCTGATAAGCCGGATTTACGTAACCCGTTAGAATTAG
TGGACGTGGCGGATATTTTAAAAGACGTTGAGTTTAAAGTATTTAACGAGCCGGCAAATTCGGCGGACGGTCGTGTAACC
GTGCTTCGTGTGCCGAACGGTGCAAGCCTTACCCGCAAACAAATTGACGAATATACCCAATTTGTCGGTATTTACGGTGC
AAAAGGCTTAGCATGGGCAAAAATCAATAATGTGAATGCTGGTATGGAAGGCATCCAAAGCCCGGTCGCAAAATTCTTAA
ACGAAGAGGTTTTCAAAGCGTTAATCGAGCGTACTAACGCAACCAGCGGCGACATCCTATTCTTCGGTGCGGATAAATGG
CAAGTCGTTACCGACTCAATGGGTGCTTTACGTCTGAAAGTCGGTCGTGATTTAGCATTAACCGATCTTTCAGCTTGGAA
ACCGCTTTGGGTAATTGACTTCCCAATGTTTGAAAAAGACGATGAAGGCAATCTTTCTGCAATGCACCACCCGTTCACTT
CACCGAAAAATTTAACGCCGGAAGAACTGGCGGCAAATCCGGTGAATGCGGTTGCGAACGCTTACGATATGGTTATTAAC
GGTTATGAAGTGGGCGGCGGTTCAGTACGTATTTACGATCCGAAAATGCAACAAACCGTATTCGGTATCCTCGGCATTAA
CGAACAAGATCAACAAGAAAAATTCGGCTTCTTATTAGACGCATTAAAATTCGGTACGCCTCCGCACGCCGGTCTTGCAT
TCGGTTTAGACCGTTTAACTATGCTTATTACCGGCACGGAAAATATTCGTGATGTCATCGCATTCCCGAAAACTACCGCA
GCGGCGTGTTTAATGACCGAAGCACCAAGTTTCGCAAACCCGCAAGCGTTGGAAGAGCTTGGTATTGCAGTATTGAAAAA
AGAAAAAGCGGAATAA

Upstream 100 bases:

>100_bases
TTATCTCCAATTTCTGCCAATTTTCCGCTATAATTTCAAAGATTTTGTCTCCGCATTTCTCACAGCGGAAAACCTATTTT
TAAATCTTAAGGATATTTTT

Downstream 100 bases:

>100_bases
AGCAAGCGGTTAAAAATTGCTGATTTTTTGCAAATGAAATACAAAAATCCTAACTCCGTTTTAGTTGTGATTTATGCGAA
AAATTCGGGAAGAGTCTTAA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MMRSHYCGALNRSHVGQTVTLSGWVHRVRNLGRFIFMQIRDREGIVQVFFDEKDEAIFKIASSLRSEACVQIQGEVIARD
ESQINKEMATGEIEVLVKNVVVYNNADVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRYMDDNGFLD
IETPMLTKATPEGARDYLVPSRVHNGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAEEVRELMENMIHGLWLDRLNVDLGKFPIMTWQEAMQRFGSDKPDLRNPLELVDVADILKDVEFKVFNEPANSADGRVT
VLRVPNGASLTRKQIDEYTQFVGIYGAKGLAWAKINNVNAGMEGIQSPVAKFLNEEVFKALIERTNATSGDILFFGADKW
QVVTDSMGALRLKVGRDLALTDLSAWKPLWVIDFPMFEKDDEGNLSAMHHPFTSPKNLTPEELAANPVNAVANAYDMVIN
GYEVGGGSVRIYDPKMQQTVFGILGINEQDQQEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTENIRDVIAFPKTTA
AACLMTEAPSFANPQALEELGIAVLKKEKAE

Sequences:

>Translated_591_residues
MMRSHYCGALNRSHVGQTVTLSGWVHRVRNLGRFIFMQIRDREGIVQVFFDEKDEAIFKIASSLRSEACVQIQGEVIARD
ESQINKEMATGEIEVLVKNVVVYNNADVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRYMDDNGFLD
IETPMLTKATPEGARDYLVPSRVHNGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAEEVRELMENMIHGLWLDRLNVDLGKFPIMTWQEAMQRFGSDKPDLRNPLELVDVADILKDVEFKVFNEPANSADGRVT
VLRVPNGASLTRKQIDEYTQFVGIYGAKGLAWAKINNVNAGMEGIQSPVAKFLNEEVFKALIERTNATSGDILFFGADKW
QVVTDSMGALRLKVGRDLALTDLSAWKPLWVIDFPMFEKDDEGNLSAMHHPFTSPKNLTPEELAANPVNAVANAYDMVIN
GYEVGGGSVRIYDPKMQQTVFGILGINEQDQQEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTENIRDVIAFPKTTA
AACLMTEAPSFANPQALEELGIAVLKKEKAE
>Mature_591_residues
MMRSHYCGALNRSHVGQTVTLSGWVHRVRNLGRFIFMQIRDREGIVQVFFDEKDEAIFKIASSLRSEACVQIQGEVIARD
ESQINKEMATGEIEVLVKNVVVYNNADVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRYMDDNGFLD
IETPMLTKATPEGARDYLVPSRVHNGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAEEVRELMENMIHGLWLDRLNVDLGKFPIMTWQEAMQRFGSDKPDLRNPLELVDVADILKDVEFKVFNEPANSADGRVT
VLRVPNGASLTRKQIDEYTQFVGIYGAKGLAWAKINNVNAGMEGIQSPVAKFLNEEVFKALIERTNATSGDILFFGADKW
QVVTDSMGALRLKVGRDLALTDLSAWKPLWVIDFPMFEKDDEGNLSAMHHPFTSPKNLTPEELAANPVNAVANAYDMVIN
GYEVGGGSVRIYDPKMQQTVFGILGINEQDQQEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTENIRDVIAFPKTTA
AACLMTEAPSFANPQALEELGIAVLKKEKAE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=600, Percent_Identity=38.5, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI194272210, Length=249, Percent_Identity=26.5060240963855, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI5031815, Length=249, Percent_Identity=26.5060240963855, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI45439306, Length=264, Percent_Identity=25.3787878787879, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI226491502, Length=256, Percent_Identity=25, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4758762, Length=254, Percent_Identity=25.5905511811024, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788173, Length=586, Percent_Identity=74.7440273037543, Blast_Score=920, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=288, Percent_Identity=26.3888888888889, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790571, Length=294, Percent_Identity=25.1700680272109, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=608, Percent_Identity=35.6907894736842, Blast_Score=328, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI17551876, Length=301, Percent_Identity=27.5747508305648, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994340, Length=252, Percent_Identity=23.8095238095238, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=252, Percent_Identity=23.8095238095238, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=252, Percent_Identity=23.8095238095238, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=312, Percent_Identity=38.1410256410256, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321807, Length=249, Percent_Identity=28.9156626506024, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=257, Percent_Identity=27.2373540856031, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=245, Percent_Identity=25.3061224489796, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24584738, Length=608, Percent_Identity=35.6907894736842, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24640851, Length=246, Percent_Identity=28.4552845528455, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640849, Length=246, Percent_Identity=28.4552845528455, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17136276, Length=241, Percent_Identity=28.2157676348548, Blast_Score=86, Evalue=1e-16,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_ACTP2 (A3N1F3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053844.1
- ProteinModelPortal:   A3N1F3
- SMR:   A3N1F3
- STRING:   A3N1F3
- GeneID:   4849850
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1149
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   A3N1F3
- ProtClustDB:   PRK00476
- BioCyc:   APLE416269:APL_1149-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66810; Mature: 66810

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRSHYCGALNRSHVGQTVTLSGWVHRVRNLGRFIFMQIRDREGIVQVFFDEKDEAIFKI
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCHHHHHHH
ASSLRSEACVQIQGEVIARDESQINKEMATGEIEVLVKNVVVYNNADVLPLDFNQNNTEE
HHHHHHCCEEEECCEEEECCHHHHHHHHHCCHHHEEEEEEEEECCCCEEEEECCCCCCHH
QRLKYRYLDLRRPEMAEKLKTRAKITSFVRRYMDDNGFLDIETPMLTKATPEGARDYLVP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
SRVHNGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
CEECCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHC
TAEEVRELMENMIHGLWLDRLNVDLGKFPIMTWQEAMQRFGSDKPDLRNPLELVDVADIL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KDVEFKVFNEPANSADGRVTVLRVPNGASLTRKQIDEYTQFVGIYGAKGLAWAKINNVNA
HHCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
GMEGIQSPVAKFLNEEVFKALIERTNATSGDILFFGADKWQVVTDSMGALRLKVGRDLAL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCEEEEECCCCEEE
TDLSAWKPLWVIDFPMFEKDDEGNLSAMHHPFTSPKNLTPEELAANPVNAVANAYDMVIN
ECCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHEEE
GYEVGGGSVRIYDPKMQQTVFGILGINEQDQQEKFGFLLDALKFGTPPHAGLAFGLDRLT
CEEECCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEE
MLITGTENIRDVIAFPKTTAAACLMTEAPSFANPQALEELGIAVLKKEKAE
EEEECCHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMRSHYCGALNRSHVGQTVTLSGWVHRVRNLGRFIFMQIRDREGIVQVFFDEKDEAIFKI
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCHHHHHHH
ASSLRSEACVQIQGEVIARDESQINKEMATGEIEVLVKNVVVYNNADVLPLDFNQNNTEE
HHHHHHCCEEEECCEEEECCHHHHHHHHHCCHHHEEEEEEEEECCCCEEEEECCCCCCHH
QRLKYRYLDLRRPEMAEKLKTRAKITSFVRRYMDDNGFLDIETPMLTKATPEGARDYLVP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
SRVHNGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
CEECCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHC
TAEEVRELMENMIHGLWLDRLNVDLGKFPIMTWQEAMQRFGSDKPDLRNPLELVDVADIL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KDVEFKVFNEPANSADGRVTVLRVPNGASLTRKQIDEYTQFVGIYGAKGLAWAKINNVNA
HHCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
GMEGIQSPVAKFLNEEVFKALIERTNATSGDILFFGADKWQVVTDSMGALRLKVGRDLAL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCEEEEECCCCEEE
TDLSAWKPLWVIDFPMFEKDDEGNLSAMHHPFTSPKNLTPEELAANPVNAVANAYDMVIN
ECCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHEEE
GYEVGGGSVRIYDPKMQQTVFGILGINEQDQQEKFGFLLDALKFGTPPHAGLAFGLDRLT
CEEECCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEE
MLITGTENIRDVIAFPKTTAAACLMTEAPSFANPQALEELGIAVLKKEKAE
EEEECCHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA