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The map label for this gene is eno

Identifier: 126208585

GI number: 126208585

Start: 1290405

End: 1291715

Strand: Direct

Name: eno

Synonym: APL_1113

Alternate gene names: 126208585

Gene position: 1290405-1291715 (Clockwise)

Preceding gene: 126208584

Following gene: 126208586

Centisome position: 56.73

GC content: 41.95

Gene sequence:

>1311_bases
ATGGCTAAAATCGTTAAAGTAATCGGTCGTGAAATCATCGACTCACGTGGTAACCCAACTGTTGAAGCGGAAGTTCACTT
AGAAGGTGGTTTCGTCGGTTTAGCGGCCGCTCCATCTGGCGCATCTACCGGTTCACGTGAAGCATTAGAATTACGTGACG
GTGATAAATCACGTTTCTTAGGTAAAGGCGTATTAAAAGCTGTTTCTGCAGTAAACAACGAAATCGCACAAGCTATCTTA
GGTAAAGACGGTTCCGCTCAAGCTGAAATCGACCAAATCATGATCGATTTAGACGGCACGGACAATAAATCTAAATTCGG
TGCAAACGCAATCTTAGCGGTTTCTTTAGCAACCGCTAAAGCGGCGGCGGCATCTAAAGGCTTACCGTTATATGCTTATA
TCGCTGAATTAAACGGTACGCCGGGCGTATATTCAATGCCGTTACCGATGATGAACATCATCAACGGTGGTGAACACGCT
GACAATAACGTTGATATCCAAGAATTTATGATTCAACCGGTTGGCGCATCAACATTAAAAGAAGCGCTTCGTATCGGTGC
GGAAGTATTCCACAACTTAGCGAAAGTATTAAAATCTAAAGGTTTAAATACTGCTGTGGGTGACGAAGGTGGTTTTGCGC
CTAACTTAGCATCAAACGCAGATGCGTTAGCATGTATCAAAGAAGCGGTTGAAAAAGCGGGTTATGTTTTAGGTAAAGAC
GTAACTTTAGCGATGGACTGCGCATCTTCTGAGTTCTATAACAAAGAAAACGGTTTATACGAAATGAAAGGTGAAGGTAA
ATCTTTCACTTCTCAAGAATTTACTCACTACTTAGAAGGTTTATGCAACGAATACCCAATCAAATCGATTGAAGACGGTC
AAGACGAATCCGACTGGGAAGGTTTTGCATATCAAACTAAAGTTTTAGGCGGTAAAATCCAATTAGTTGGTGACGACTTA
TTCGTAACTAACACCAAAATCTTAAAAGAAGGTATCGAAAAAGGTATCGCAAACTCAATCTTAATCAAATTCAACCAAAT
CGGTTCATTAACCGAAACTTTAGCGGCAATCAAAATGGCTAAAGATGCCGGTTATACCGCAGTTATCTCACACCGTTCTG
GTGAAACTGAAGATGCGACTATCGCGGATTTAGCGGTTGGTACGGCTGCAGGTCAAATCAAAACAGGTTCTATGAGCCGT
TCAGACCGTGTTGCTAAATACAACCAATTAATTCGTATCGAAGAAGCATTAGCGGCAGCAGGTACGCCTGCACCGTTTAA
CGGTTTAAAAGAAGTTAAAGGTCAAGCGTAA

Upstream 100 bases:

>100_bases
TTTTGTGAGCTAAATCACTTTTCTACAAGACAAAAGCGTAAAATATCTTTACTATATCACTATTCTTTTTTTATTAACTC
TCAATTAGAGGATATTAACA

Downstream 100 bases:

>100_bases
TCTTATTTGATTCGTAAGTAATAAATGCCACAGAAATTTCTGTGGCATTTTTGTAATTTTTACTTGGTATTCCTTATGCA
AGAAGTTCTCCTTTCCGAGC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVSAVNNEIAQAIL
GKDGSAQAEIDQIMIDLDGTDNKSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKSKGLNTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKD
VTLAMDCASSEFYNKENGLYEMKGEGKSFTSQEFTHYLEGLCNEYPIKSIEDGQDESDWEGFAYQTKVLGGKIQLVGDDL
FVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRVAKYNQLIRIEEALAAAGTPAPFNGLKEVKGQA

Sequences:

>Translated_436_residues
MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVSAVNNEIAQAIL
GKDGSAQAEIDQIMIDLDGTDNKSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKSKGLNTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKD
VTLAMDCASSEFYNKENGLYEMKGEGKSFTSQEFTHYLEGLCNEYPIKSIEDGQDESDWEGFAYQTKVLGGKIQLVGDDL
FVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRVAKYNQLIRIEEALAAAGTPAPFNGLKEVKGQA
>Mature_435_residues
AKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVSAVNNEIAQAILG
KDGSAQAEIDQIMIDLDGTDNKSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHAD
NNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKSKGLNTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKDV
TLAMDCASSEFYNKENGLYEMKGEGKSFTSQEFTHYLEGLCNEYPIKSIEDGQDESDWEGFAYQTKVLGGKIQLVGDDLF
VTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRS
DRVAKYNQLIRIEEALAAAGTPAPFNGLKEVKGQA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=53.7931034482759, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=53.7931034482759, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI5803011, Length=434, Percent_Identity=50.9216589861751, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=50.6912442396313, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=48.729792147806, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=341, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI169201757, Length=341, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI239744207, Length=341, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789141, Length=437, Percent_Identity=84.4393592677345, Blast_Score=723, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71995829, Length=435, Percent_Identity=52.8735632183908, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=435, Percent_Identity=52.8735632183908, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32563855, Length=194, Percent_Identity=45.360824742268, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6321693, Length=425, Percent_Identity=49.1764705882353, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323985, Length=425, Percent_Identity=47.5294117647059, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324974, Length=425, Percent_Identity=47.5294117647059, Blast_Score=358, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324969, Length=425, Percent_Identity=47.5294117647059, Blast_Score=358, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6321968, Length=425, Percent_Identity=48.4705882352941, Blast_Score=335, Evalue=8e-93,
Organism=Drosophila melanogaster, GI24580918, Length=426, Percent_Identity=51.1737089201878, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580916, Length=426, Percent_Identity=51.1737089201878, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580920, Length=426, Percent_Identity=51.1737089201878, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580914, Length=426, Percent_Identity=51.1737089201878, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI281360527, Length=420, Percent_Identity=51.6666666666667, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI17137654, Length=420, Percent_Identity=51.6666666666667, Blast_Score=371, Evalue=1e-103,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_ACTP2 (A3N1B9)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053810.1
- ProteinModelPortal:   A3N1B9
- SMR:   A3N1B9
- STRING:   A3N1B9
- GeneID:   4849738
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1113
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   A3N1B9
- ProtClustDB:   PRK00077
- BioCyc:   APLE416269:APL_1113-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45857; Mature: 45726

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 343-343 BINDING 159-159 BINDING 168-168 BINDING 291-291 BINDING 318-318 BINDING 343-343 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKSRFL
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVSAVNNEIAQAILGKDGSAQAEIDQIMIDLDGTDNKSKFGANAILAVSLATAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEHHHHH
AAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHADNNVDIQEFMIQPVGASTLK
HHHHCCCCEEEEEEEECCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRIGAEVFHNLAKVLKSKGLNTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLAMDCASSEFYNKENGLYEMKGEGKSFTSQEFTHYLEGLCNEYPIKSIEDGQDESDWE
EEEEEECCCCHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GFAYQTKVLGGKIQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMA
CEEEEEEECCCEEEEEECCEEECHHHHHHHHHHHCCCHHEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALAAA
HCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHC
GTPAPFNGLKEVKGQA
CCCCCCCCHHHHCCCC
>Mature Secondary Structure 
AKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKSRFL
HHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVSAVNNEIAQAILGKDGSAQAEIDQIMIDLDGTDNKSKFGANAILAVSLATAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEHHHHH
AAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHADNNVDIQEFMIQPVGASTLK
HHHHCCCCEEEEEEEECCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRIGAEVFHNLAKVLKSKGLNTAVGDEGGFAPNLASNADALACIKEAVEKAGYVLGKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLAMDCASSEFYNKENGLYEMKGEGKSFTSQEFTHYLEGLCNEYPIKSIEDGQDESDWE
EEEEEECCCCHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GFAYQTKVLGGKIQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMA
CEEEEEEECCCEEEEEECCEEECHHHHHHHHHHHCCCHHEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALAAA
HCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHC
GTPAPFNGLKEVKGQA
CCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA