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The map label for this gene is aspA [H]

Identifier: 126208563

GI number: 126208563

Start: 1261851

End: 1263278

Strand: Reverse

Name: aspA [H]

Synonym: APL_1091

Alternate gene names: 126208563

Gene position: 1263278-1261851 (Counterclockwise)

Preceding gene: 126208564

Following gene: 126208562

Centisome position: 55.54

GC content: 39.85

Gene sequence:

>1428_bases
ATGAGTAATGTACGTGTTGAAGTGGATTTATTAGGCGAAAGAGAAGTACCTGCAGAAGCTTATTGGGGTATCCACACACT
AAGAGCGGTTGAGAATTTTAATATTTCAGAAAATACTATTTCGGATGTACCGGAATTTGTTCGCGGTATGGTAATGGTAA
AAAAAGCGACCGCATTAGCGAACGGTGAATTAGGTGCGATTCCGAAAAAAATTGCGAAAGCGATTGTCGAGGCTTGTGAC
GAAATTTTAGTGAAAGGCCGCTGTATGGATCAGTTCCCATCAGATGTATATCAAGGTGGTGCCGGTACATCGGTTAATAT
GAATACTAATGAAGTAGTAGCAAATTTAGCTTTAGAGTTATTAGGTCATAAAAAAGGCGAATATAGCGTGATTAACCCGA
TGGATCATGTGAATGCAAGCCAATCAACCAATGATGCTTATCCGACCGGTTTCCGTATTGCGGTCTATAACAGTATCTTG
CATTTAATTAAAAAAGTCCGTTATTTACAAAAAGGCTTCGAAGCAAAAGCGGAAGAATTTAAAGACGTGTTAAAAATGGG
CCGTACTCAGTTACAAGATGCGGTACCAATGACAGTCGGTCAGGAATTTAAAGCTTTCGCTGTGTTATTGGAAGAAGAAG
TACGTAATTTAAAACGTACCGCTAAATTATTGTTAGAAGTGAATTTAGGCGCAACCGCAATCGGTACAGGTTTAAATACA
CCGGAAGGTTATTCGGAATTAGTGGTTAAACATTTATCGGAAGTAACCTGTTTACCTTGTGTTTTATCAGAGAACTTAAT
TGAAGCGACTTCAGACTGTGGTGCTTATGTAATGGTTCACGGTGCGTTAAAACGTACGGCGGTTAAACTTTCAAAAGTAT
GTAACGACTTACGTTTACTTTCATCAGGCCCACGTGCCGGTTTAAATGAAATCAATTTACCGGAATTACAAGCAGGTTCT
TCAATTATGCCGGCAAAAGTAAACCCGGTTGTGCCGGAAGTTGTGAACCAAGTTTGTTTTAAAGTAATTGGTAATGACAC
GACCGTAACCTTTGCAGCGGAAGCAGGTCAGTTACAATTAAACGTAATGGAACCGGTAATCGGTCAAGCGATGTTTGAAT
CTATCGACATTCTTGCCAACGCTTGTGTGAATTTACGTGATAAATGTATCGACGGCATCACGGTCAATAAAGAAATCTGT
GAAAACTTCGTATTTAATTCAATCGGTATCGTCACTTACCTCAATCCGTTTATCGGTCACCATAACGGTGATATTGTTGG
TAAAATCTGTGCAACAACCGGCAGAGGCGTACGTGAAGTGGTATTAGAACGCGGCTTACTCACAGAAGCGCAATTAGACG
ATATTCTTTCAGTTGAAAACTTAATGAACCCGACTTATAAAGCGGCGAAATTTACTGAAGAAGAGTAG

Upstream 100 bases:

>100_bases
GTCTATAAAATGTGATCTAAATCACATTTCTTTTTATACAAAATCATTATGATCTCCACCAAATTTAGTACCTGTTATTT
TAATTTATTGAGGTGAATTT

Downstream 100 bases:

>100_bases
TTTCAGCTTAGTTTTATAAACTGACAAGCGGTCGTATTTTGTTATTTTTTGCAAAGACGACCGCTATTTTTTTATTGAAT
ATTTCAATGGTTATTGAAAT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSNVRVEVDLLGEREVPAEAYWGIHTLRAVENFNISENTISDVPEFVRGMVMVKKATALANGELGAIPKKIAKAIVEACD
EILVKGRCMDQFPSDVYQGGAGTSVNMNTNEVVANLALELLGHKKGEYSVINPMDHVNASQSTNDAYPTGFRIAVYNSIL
HLIKKVRYLQKGFEAKAEEFKDVLKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAKLLLEVNLGATAIGTGLNT
PEGYSELVVKHLSEVTCLPCVLSENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGS
SIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIDILANACVNLRDKCIDGITVNKEIC
ENFVFNSIGIVTYLNPFIGHHNGDIVGKICATTGRGVREVVLERGLLTEAQLDDILSVENLMNPTYKAAKFTEEE

Sequences:

>Translated_475_residues
MSNVRVEVDLLGEREVPAEAYWGIHTLRAVENFNISENTISDVPEFVRGMVMVKKATALANGELGAIPKKIAKAIVEACD
EILVKGRCMDQFPSDVYQGGAGTSVNMNTNEVVANLALELLGHKKGEYSVINPMDHVNASQSTNDAYPTGFRIAVYNSIL
HLIKKVRYLQKGFEAKAEEFKDVLKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAKLLLEVNLGATAIGTGLNT
PEGYSELVVKHLSEVTCLPCVLSENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGS
SIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIDILANACVNLRDKCIDGITVNKEIC
ENFVFNSIGIVTYLNPFIGHHNGDIVGKICATTGRGVREVVLERGLLTEAQLDDILSVENLMNPTYKAAKFTEEE
>Mature_474_residues
SNVRVEVDLLGEREVPAEAYWGIHTLRAVENFNISENTISDVPEFVRGMVMVKKATALANGELGAIPKKIAKAIVEACDE
ILVKGRCMDQFPSDVYQGGAGTSVNMNTNEVVANLALELLGHKKGEYSVINPMDHVNASQSTNDAYPTGFRIAVYNSILH
LIKKVRYLQKGFEAKAEEFKDVLKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAKLLLEVNLGATAIGTGLNTP
EGYSELVVKHLSEVTCLPCVLSENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGSS
IMPAKVNPVVPEVVNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIDILANACVNLRDKCIDGITVNKEICE
NFVFNSIGIVTYLNPFIGHHNGDIVGKICATTGRGVREVVLERGLLTEAQLDDILSVENLMNPTYKAAKFTEEE

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=464, Percent_Identity=37.9310344827586, Blast_Score=322, Evalue=5e-88,
Organism=Escherichia coli, GI87082375, Length=473, Percent_Identity=78.2241014799154, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=37.7969762419007, Blast_Score=330, Evalue=8e-92,
Organism=Escherichia coli, GI1790398, Length=282, Percent_Identity=23.0496453900709, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17553882, Length=465, Percent_Identity=41.2903225806452, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=41.1042944785276, Blast_Score=260, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324993, Length=464, Percent_Identity=39.8706896551724, Blast_Score=337, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=39.9132321041215, Blast_Score=341, Evalue=5e-94,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=39.9132321041215, Blast_Score=341, Evalue=6e-94,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=39.1397849462366, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=38.8768898488121, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24583245, Length=450, Percent_Identity=33.3333333333333, Blast_Score=271, Evalue=9e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51683; Mature: 51552

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVRVEVDLLGEREVPAEAYWGIHTLRAVENFNISENTISDVPEFVRGMVMVKKATALA
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
NGELGAIPKKIAKAIVEACDEILVKGRCMDQFPSDVYQGGAGTSVNMNTNEVVANLALEL
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCCCEECCCHHHHHHHHHHHH
LGHKKGEYSVINPMDHVNASQSTNDAYPTGFRIAVYNSILHLIKKVRYLQKGFEAKAEEF
HCCCCCCEEEECCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDVLKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAKLLLEVNLGATAIGTGLNT
HHHHHHHHHHHHHCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCC
PEGYSELVVKHLSEVTCLPCVLSENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFAAEAGQLQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEE
NVMEPVIGQAMFESIDILANACVNLRDKCIDGITVNKEICENFVFNSIGIVTYLNPFIGH
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHCC
HNGDIVGKICATTGRGVREVVLERGLLTEAQLDDILSVENLMNPTYKAAKFTEEE
CCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure 
SNVRVEVDLLGEREVPAEAYWGIHTLRAVENFNISENTISDVPEFVRGMVMVKKATALA
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
NGELGAIPKKIAKAIVEACDEILVKGRCMDQFPSDVYQGGAGTSVNMNTNEVVANLALEL
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCCCEECCCHHHHHHHHHHHH
LGHKKGEYSVINPMDHVNASQSTNDAYPTGFRIAVYNSILHLIKKVRYLQKGFEAKAEEF
HCCCCCCEEEECCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDVLKMGRTQLQDAVPMTVGQEFKAFAVLLEEEVRNLKRTAKLLLEVNLGATAIGTGLNT
HHHHHHHHHHHHHCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCC
PEGYSELVVKHLSEVTCLPCVLSENLIEATSDCGAYVMVHGALKRTAVKLSKVCNDLRLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTFAAEAGQLQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEE
NVMEPVIGQAMFESIDILANACVNLRDKCIDGITVNKEICENFVFNSIGIVTYLNPFIGH
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHCC
HNGDIVGKICATTGRGVREVVLERGLLTEAQLDDILSVENLMNPTYKAAKFTEEE
CCCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]