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The map label for this gene is xthA [H]

Identifier: 126208451

GI number: 126208451

Start: 1130902

End: 1131705

Strand: Direct

Name: xthA [H]

Synonym: APL_0977

Alternate gene names: 126208451

Gene position: 1130902-1131705 (Clockwise)

Preceding gene: 126208450

Following gene: 126208455

Centisome position: 49.72

GC content: 42.91

Gene sequence:

>804_bases
ATGAAATTTATTTCTTTTAATATTAACGGCTTACGCGCTCGCCCTCATCAATTGGAAGCGATTATTGAAAAACATCAACC
GGATGTACTCGGCTTACAAGAAATCAAAGTCGCTGATGAAGATTTCCCTTGGGACTTAGTCAATCATTTAGGCTACCACG
TGTTTCATCACGGTCAAAAAGGGCATTACGGCGTGGCGCTTTTAACCAAAAAAGAACCGCTTGCCGTGCGTAAGGGCTTT
CCGACCGATAATGCCGATGCGCAAAAACGTATGATTATGGCGGATATTGAAACGGAATTCGGCGTGCTGACCGTGTTAAA
CGGTTATTTCCCACAAGGTGAAAATCGCTCGCACGAAACCAAATTTCCGGCAAAAGAAAAATTTTATGCGGATTTACAAC
ATTACTTAGAAAGCGAACTGTCGCCGGAAAATCCGATTGTGATTATGGGCGATATGAATATCAGTCCGACCGATTTAGAT
ATCGGTATCGGTGAGCCGAATCGCAAACGTTGGCTACGTGACGGTAAATGTTCATTCCTACCGGAAGAACGTGAATGGTT
AGATCGTTTATATAGTTACGGTTTAATCGATACATTCCGTGCGATGAACCCGCAAGCTAATGATAAATTCTCGTGGTTTG
ATTACCGTTCGAAAGGATTTGATGATAATCGAGGACTACGTATTGATTTGGTACTCGCTTCAAAAGGATTAGCGGAACGT
TGTGTAGAAACCGGCATTGATCTTGAAATTCGCGCAATGGAAAAACCGTCGGATCATTCTCCGGTTTGGGCGGTATTCAA
ATAG

Upstream 100 bases:

>100_bases
GATGCTTTAGTCTTTTTGCTTATACCACGGATAATGCAAATTTTTTCGGAAATATAACCGCTTGTTTGCTTTATCCGTGG
TTTCTATTTGGATTTTACGT

Downstream 100 bases:

>100_bases
CAAACAAGCGGTCAAATTTAGCAAAAAATTTACAAAAAATTCTTTAAATTTGACCGCTTGTCTTTAATCTTTCGGCAACA
ATTCCCAAAATGCCTTAATA

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPWDLVNHLGYHVFHHGQKGHYGVALLTKKEPLAVRKGF
PTDNADAQKRMIMADIETEFGVLTVLNGYFPQGENRSHETKFPAKEKFYADLQHYLESELSPENPIVIMGDMNISPTDLD
IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLIDTFRAMNPQANDKFSWFDYRSKGFDDNRGLRIDLVLASKGLAER
CVETGIDLEIRAMEKPSDHSPVWAVFK

Sequences:

>Translated_267_residues
MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPWDLVNHLGYHVFHHGQKGHYGVALLTKKEPLAVRKGF
PTDNADAQKRMIMADIETEFGVLTVLNGYFPQGENRSHETKFPAKEKFYADLQHYLESELSPENPIVIMGDMNISPTDLD
IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLIDTFRAMNPQANDKFSWFDYRSKGFDDNRGLRIDLVLASKGLAER
CVETGIDLEIRAMEKPSDHSPVWAVFK
>Mature_267_residues
MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPWDLVNHLGYHVFHHGQKGHYGVALLTKKEPLAVRKGF
PTDNADAQKRMIMADIETEFGVLTVLNGYFPQGENRSHETKFPAKEKFYADLQHYLESELSPENPIVIMGDMNISPTDLD
IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLIDTFRAMNPQANDKFSWFDYRSKGFDDNRGLRIDLVLASKGLAER
CVETGIDLEIRAMEKPSDHSPVWAVFK

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=269, Percent_Identity=26.7657992565056, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI18375503, Length=269, Percent_Identity=26.7657992565056, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI18375501, Length=269, Percent_Identity=26.7657992565056, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=74.9063670411985, Blast_Score=432, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71989536, Length=266, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI221330655, Length=269, Percent_Identity=30.1115241635688, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17136678, Length=269, Percent_Identity=30.1115241635688, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30747; Mature: 30747

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPWDLVNHLGYHVFHHGQK
CEEEEEECCCEECCHHHHHHHHHHCCCCEECHHHEEECCCCCCHHHHHHCCHHEEECCCC
GHYGVALLTKKEPLAVRKGFPTDNADAQKRMIMADIETEFGVLTVLNGYFPQGENRSHET
CCEEEEEEECCCCCCEECCCCCCCCCHHHEEEEEECCCCCCEEEEECCCCCCCCCCCCCC
KFPAKEKFYADLQHYLESELSPENPIVIMGDMNISPTDLDIGIGEPNRKRWLRDGKCSFL
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCHHHHCCCCCCCCC
PEEREWLDRLYSYGLIDTFRAMNPQANDKFSWFDYRSKGFDDNRGLRIDLVLASKGLAER
CHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCHHHH
CVETGIDLEIRAMEKPSDHSPVWAVFK
HHHCCCCEEEEEECCCCCCCCEEEEEC
>Mature Secondary Structure
MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPWDLVNHLGYHVFHHGQK
CEEEEEECCCEECCHHHHHHHHHHCCCCEECHHHEEECCCCCCHHHHHHCCHHEEECCCC
GHYGVALLTKKEPLAVRKGFPTDNADAQKRMIMADIETEFGVLTVLNGYFPQGENRSHET
CCEEEEEEECCCCCCEECCCCCCCCCHHHEEEEEECCCCCCEEEEECCCCCCCCCCCCCC
KFPAKEKFYADLQHYLESELSPENPIVIMGDMNISPTDLDIGIGEPNRKRWLRDGKCSFL
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCHHHHCCCCCCCCC
PEEREWLDRLYSYGLIDTFRAMNPQANDKFSWFDYRSKGFDDNRGLRIDLVLASKGLAER
CHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCHHHH
CVETGIDLEIRAMEKPSDHSPVWAVFK
HHHCCCCEEEEEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]