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The map label for this gene is ttcA

Identifier: 126208449

GI number: 126208449

Start: 1128272

End: 1129228

Strand: Direct

Name: ttcA

Synonym: APL_0975

Alternate gene names: 126208449

Gene position: 1128272-1129228 (Clockwise)

Preceding gene: 126208448

Following gene: 126208450

Centisome position: 49.61

GC content: 40.86

Gene sequence:

>957_bases
ATGAATCAAGATACTCAATCAGCGAATACTCAACAAGAAAAAAAGATTGCCTATAATTTCAACAAATTACAAAAACGTTT
ACGCCGTAATGTCGGTAATGCGATTGCCGATTTCAATATGATTGAAGACGGTGACAAAGTAATGGTGTGTTTATCCGGCG
GTAAAGACAGCTACACATTATTAGATATTCTACTTAATCTCAAACTCAGCGCACCGATTCACTTCGATATCGTAGCGGTT
AATTTAGACCAAAAACAACCCGGCTTTCCGGAACATATTTTGCCTGAATATCTGGAATCTATCGGCGTTGAATATAAAAT
CGTCGAAGAAAATACCTACGGCATCGTAAAAGAGAAAATTCCGGAAGGGAAAACCACTTGCTCGCTTTGCTCGCGTTTAC
GTCGCGGCATTTTATACCGCACCGCTACCGAACTCGGTGCAACAAAAATTGCGCTTGGCCACCACCGTGACGATATGTTG
GAAACGTTATTCTTAAATATGTTTTACGGCGGTAAATTAAAATCTATGCCGCCTAAATTGATTTCCGATGATGGCAAACA
AATCGTGATTCGTCCGTTGGCTTATTGTAAAGAAAAAGATATCGAAAAATATTCGCAGGCAAAACAATTCCCGATTATTC
CGTGTAACTTATGCGGTTCTCAGCCGAATCTACAGCGCCAAGTGGTTAAAGAAATGCTCCAGACATGGGATCGCCAATAT
CCGGGACGAATCGAAACGATGTTTAGCGCAATGCAAAACATTACGCTTTCACATCTTTGTGATCCGAGTCTGTTCGATTT
TAAAGGATTAAAACGCGGTCAGGTATTGGACGGCGTGGAAGGCGATATTGCTTTCGATAAAGCGGAAATCCCAAATCAAC
CGTTAATTCAAGATGAAGACGAACAAACCACCGACTACGGCGAAAACGGTATGATTCAATTTAAACAGGTTCAATAA

Upstream 100 bases:

>100_bases
CCAATGAAACGCTTGCAAAAAATCCGGAAGTCTTTAGTATGACGGACTTCATTTTTTATCTCATATACCGAACCGAACAT
TTATTTAAAAATTCACGATT

Downstream 100 bases:

>100_bases
TCCTAAAGAGCAAACTTTTAAGTTTGCTCTTTTTTCTATAACTTGTAGCTGAAACATAAAATCTTCAGTAAAAAAGCTAA
AACGACAAGATTTATAGCGA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNQDTQSANTQQEKKIAYNFNKLQKRLRRNVGNAIADFNMIEDGDKVMVCLSGGKDSYTLLDILLNLKLSAPIHFDIVAV
NLDQKQPGFPEHILPEYLESIGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDML
ETLFLNMFYGGKLKSMPPKLISDDGKQIVIRPLAYCKEKDIEKYSQAKQFPIIPCNLCGSQPNLQRQVVKEMLQTWDRQY
PGRIETMFSAMQNITLSHLCDPSLFDFKGLKRGQVLDGVEGDIAFDKAEIPNQPLIQDEDEQTTDYGENGMIQFKQVQ

Sequences:

>Translated_318_residues
MNQDTQSANTQQEKKIAYNFNKLQKRLRRNVGNAIADFNMIEDGDKVMVCLSGGKDSYTLLDILLNLKLSAPIHFDIVAV
NLDQKQPGFPEHILPEYLESIGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDML
ETLFLNMFYGGKLKSMPPKLISDDGKQIVIRPLAYCKEKDIEKYSQAKQFPIIPCNLCGSQPNLQRQVVKEMLQTWDRQY
PGRIETMFSAMQNITLSHLCDPSLFDFKGLKRGQVLDGVEGDIAFDKAEIPNQPLIQDEDEQTTDYGENGMIQFKQVQ
>Mature_318_residues
MNQDTQSANTQQEKKIAYNFNKLQKRLRRNVGNAIADFNMIEDGDKVMVCLSGGKDSYTLLDILLNLKLSAPIHFDIVAV
NLDQKQPGFPEHILPEYLESIGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDML
ETLFLNMFYGGKLKSMPPKLISDDGKQIVIRPLAYCKEKDIEKYSQAKQFPIIPCNLCGSQPNLQRQVVKEMLQTWDRQY
PGRIETMFSAMQNITLSHLCDPSLFDFKGLKRGQVLDGVEGDIAFDKAEIPNQPLIQDEDEQTTDYGENGMIQFKQVQ

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=300, Percent_Identity=77.3333333333333, Blast_Score=489, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI193211090, Length=236, Percent_Identity=31.7796610169492, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=237, Percent_Identity=26.5822784810127, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24651972, Length=233, Percent_Identity=27.8969957081545, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_ACTP2 (A3N0Y3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053674.1
- ProteinModelPortal:   A3N0Y3
- SMR:   A3N0Y3
- STRING:   A3N0Y3
- GeneID:   4848931
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0975
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- PhylomeDB:   A3N0Y3
- ProtClustDB:   PRK10696
- BioCyc:   APLE416269:APL_0975-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 36145; Mature: 36145

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQDTQSANTQQEKKIAYNFNKLQKRLRRNVGNAIADFNMIEDGDKVMVCLSGGKDSYTL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEEECCCCCHHH
LDILLNLKLSAPIHFDIVAVNLDQKQPGFPEHILPEYLESIGVEYKIVEENTYGIVKEKI
HHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHC
PEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLETLFLNMFYGGKLKSMPPKL
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHH
ISDDGKQIVIRPLAYCKEKDIEKYSQAKQFPIIPCNLCGSQPNLQRQVVKEMLQTWDRQY
HCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCCCCHHHHHHHHHHHHHHHCC
PGRIETMFSAMQNITLSHLCDPSLFDFKGLKRGQVLDGVEGDIAFDKAEIPNQPLIQDED
CCHHHHHHHHHHHCHHHHCCCCCCHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCC
EQTTDYGENGMIQFKQVQ
CCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MNQDTQSANTQQEKKIAYNFNKLQKRLRRNVGNAIADFNMIEDGDKVMVCLSGGKDSYTL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEEECCCCCHHH
LDILLNLKLSAPIHFDIVAVNLDQKQPGFPEHILPEYLESIGVEYKIVEENTYGIVKEKI
HHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHC
PEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLETLFLNMFYGGKLKSMPPKL
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHH
ISDDGKQIVIRPLAYCKEKDIEKYSQAKQFPIIPCNLCGSQPNLQRQVVKEMLQTWDRQY
HCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCCCCHHHHHHHHHHHHHHHCC
PGRIETMFSAMQNITLSHLCDPSLFDFKGLKRGQVLDGVEGDIAFDKAEIPNQPLIQDED
CCHHHHHHHHHHHCHHHHCCCCCCHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCC
EQTTDYGENGMIQFKQVQ
CCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA