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The map label for this gene is ostA

Identifier: 126208436

GI number: 126208436

Start: 1113814

End: 1116150

Strand: Reverse

Name: ostA

Synonym: APL_0962

Alternate gene names: 126208436

Gene position: 1116150-1113814 (Counterclockwise)

Preceding gene: 126208437

Following gene: 126208435

Centisome position: 49.07

GC content: 41.04

Gene sequence:

>2337_bases
ATGAAAACACGTTATAGTGTTCTTTCTGTTGCAATGACGGCAGCATTTTACACTCAATATGCGCAGGCGGATTTGAGAGA
ACAATGCCTATTGGGCGTACCGCATTTTCAGGGCGAAGAGGTAACGGGCGATCAAACTATGATGCCGATTGAAATTGAAG
CGGATAATGCGGTTATCAATCAGCCTAAAGATGCGACCTATACCGGTGATGTAGCGATTAAACAAGGTAATCGTTCTCTT
TTTGCGGATGAAGTAAGGGTTGAGCAAAACGGCGAGCAAGAACGCCGAGCGTTTTTGAAAGGCAGTTACCGATATCAAGA
TAATCTGATTCAAGCGCACGGACGAGATGCGGCGATGGATCTCGGCTCCGAAACGGCAGAGTTGCAAAATACCGAATTCC
AATTAGTCGGTCGTCAAGGACGAGGTACGGCGGAAAGCGGTTCGTTTAATCACAATAAACGCATTTTAAAAAATGCGACC
TTTACCGCCTGTTTACCGAATGACAATGCGTGGTCAATTGAAGGAAATGAAATGATTCAGCATATCGATGAAGAGTATGC
TGAAATTTGGCATGCGCGTTTTAAAGTATTGGGTATGCCGGTATTTTATTCGCCTTATTTACAATTTCCGATTGGCGATC
GCCGCCGTTCGGGGTTATTGATTCCGAATTTTCATCGTTCAAGTAAGGACGGTTTTGCTTATTCTCAGCCGTTTTATTGG
AATATCGCACCGAATATGGATGCGACAATTACACCGACTTATTATTCGCGCCGAGGTTGGCAAATTAGCCCTGAATACCG
TTATTTAACCAAATTGGGAGAAGGCATTGTTGCCGGTGAATATATCGGAAAAGATCGTCTAGACGAATATAGACCGGATG
ATAATGATCGCAAGCGTTACTTAATGCACTGGCGTCACAATATGAGTTTCTTAACCGGCTGGCGTTTGTATGTGGATTAT
ACCAAAGTCAGCGACAAGCGTTATTTCTCCGATTTCGATTCGGAATACGGTAGCAGTACCGACGGTTATGCCACTCAACA
ATTTAAATTAGGTTACTATCAACCGAATTATAATTTATCGATTTCCGGTAAGAAATTCCAAACCTTTGACGAATTGGATG
TCGGTCCGTATCGCGTATTACCGCAAATAGATTTTAACTACTATAACGATGAGTTGGTAAAAGGCGGAGATTTTAAACTC
TTTGCTCAGACGGCTCGTTTTGAGAATGACAGTAAGTTAATGCCGAAAGCGTGGCGTTTTCACGTAGAACCGACGCTTAA
TTTCCCACTTGCGAATCGCTACGGCAGCTTGAATTTTGAGACGAAATTGTATGCGACTCACTATCTTCAAGAAAAAGGCA
GTAGTAAGCAAGCGGATGATATGGACAAGAACGTTACTCGAATTATTCCGCAAGTAAAAGTGGATTTACAAACGGTACTG
GAAGCGGATAAGCAATTATTTAAAGGTTTTAATCAAACCTTTGAGCCGAGAGTACAATACGTTTATCGTCCGTATAAAGA
TCAAAGTAATATCGGCTCAGGCTTAAATCAAAGCGTAAGTTTCGGTTATGATTCGGCATTATTGCAAAGCGATTATTTTT
CTCTATTTAATGATCGTCGATATAGTGGTTTAGACCGTATTTCTTCTGCTAATTTAATTACTGCCGGTGGCACGAATCGT
TTCTTCAATGAAAAAACAGGGGTGGAAGTATTTAACTTTAGTATAGGACAGACTTATTACTTATCTCCATCTAAAATTGA
TGATTTATCGCAGAATAGTACAACAAAACGTTCGTCTTCATGGGCATTGGAGTCCAACTGGAAATTCCACCGTAAATGGA
ACTGGCACGGTGCTTATCAGTATGATACGCGTTTAAATCAAACTTCGTTGGCTAATACGTCATTACAATACAAGCCGAGC
CAAGATAAACTTGTTCAATTGAGTTATCGCTTTGCGAGTAAAGATTATATTAACCAGAACTTACGCAGTAATACGTATGG
GCAAGATATTAAACAAGTCGGTGCGGTTGTAGGTTGGGAACTAACGGATCGTGTCGCATTTATGGCAAGTCATTATCATG
ATATCGCACTTAAAAAGCCGGTAGAAAGCCAACTTTCCGTAAACTATAACACTTGTTGCTGGAGTGCTAACGTATATGTA
GCGCGTAAACTGACCGCAACGCCAATCGGATCGCCGGATACGATCAATGATTTATATTACGATAATAAATTCGGGGTGAA
TTTTGAATTACGCTTCGGTACGAACTACAGTAGCGGCGTACGTAAAATGCTGAAAAAAGGTATGATTCCTTATACGGAGC
AATATGGAATTAATTAA

Upstream 100 bases:

>100_bases
CAAGACAATTGGCGAAAAGACAACAATTTTCGAGAATTATTTAAGAGCAAGCGGTCATATTTTCGAACCATTTATAGCTT
TCTATTGACAAGGAATCATT

Downstream 100 bases:

>100_bases
TTTTTAATTAGTTTTGCAATAAGGATTTTATTTTATGTATTAAGAGTCGATCCTAGAATGATAGTGATCGACTCTTTTAT
TTTATATATATTAGTATATA

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein

Number of amino acids: Translated: 778; Mature: 778

Protein sequence:

>778_residues
MKTRYSVLSVAMTAAFYTQYAQADLREQCLLGVPHFQGEEVTGDQTMMPIEIEADNAVINQPKDATYTGDVAIKQGNRSL
FADEVRVEQNGEQERRAFLKGSYRYQDNLIQAHGRDAAMDLGSETAELQNTEFQLVGRQGRGTAESGSFNHNKRILKNAT
FTACLPNDNAWSIEGNEMIQHIDEEYAEIWHARFKVLGMPVFYSPYLQFPIGDRRRSGLLIPNFHRSSKDGFAYSQPFYW
NIAPNMDATITPTYYSRRGWQISPEYRYLTKLGEGIVAGEYIGKDRLDEYRPDDNDRKRYLMHWRHNMSFLTGWRLYVDY
TKVSDKRYFSDFDSEYGSSTDGYATQQFKLGYYQPNYNLSISGKKFQTFDELDVGPYRVLPQIDFNYYNDELVKGGDFKL
FAQTARFENDSKLMPKAWRFHVEPTLNFPLANRYGSLNFETKLYATHYLQEKGSSKQADDMDKNVTRIIPQVKVDLQTVL
EADKQLFKGFNQTFEPRVQYVYRPYKDQSNIGSGLNQSVSFGYDSALLQSDYFSLFNDRRYSGLDRISSANLITAGGTNR
FFNEKTGVEVFNFSIGQTYYLSPSKIDDLSQNSTTKRSSSWALESNWKFHRKWNWHGAYQYDTRLNQTSLANTSLQYKPS
QDKLVQLSYRFASKDYINQNLRSNTYGQDIKQVGAVVGWELTDRVAFMASHYHDIALKKPVESQLSVNYNTCCWSANVYV
ARKLTATPIGSPDTINDLYYDNKFGVNFELRFGTNYSSGVRKMLKKGMIPYTEQYGIN

Sequences:

>Translated_778_residues
MKTRYSVLSVAMTAAFYTQYAQADLREQCLLGVPHFQGEEVTGDQTMMPIEIEADNAVINQPKDATYTGDVAIKQGNRSL
FADEVRVEQNGEQERRAFLKGSYRYQDNLIQAHGRDAAMDLGSETAELQNTEFQLVGRQGRGTAESGSFNHNKRILKNAT
FTACLPNDNAWSIEGNEMIQHIDEEYAEIWHARFKVLGMPVFYSPYLQFPIGDRRRSGLLIPNFHRSSKDGFAYSQPFYW
NIAPNMDATITPTYYSRRGWQISPEYRYLTKLGEGIVAGEYIGKDRLDEYRPDDNDRKRYLMHWRHNMSFLTGWRLYVDY
TKVSDKRYFSDFDSEYGSSTDGYATQQFKLGYYQPNYNLSISGKKFQTFDELDVGPYRVLPQIDFNYYNDELVKGGDFKL
FAQTARFENDSKLMPKAWRFHVEPTLNFPLANRYGSLNFETKLYATHYLQEKGSSKQADDMDKNVTRIIPQVKVDLQTVL
EADKQLFKGFNQTFEPRVQYVYRPYKDQSNIGSGLNQSVSFGYDSALLQSDYFSLFNDRRYSGLDRISSANLITAGGTNR
FFNEKTGVEVFNFSIGQTYYLSPSKIDDLSQNSTTKRSSSWALESNWKFHRKWNWHGAYQYDTRLNQTSLANTSLQYKPS
QDKLVQLSYRFASKDYINQNLRSNTYGQDIKQVGAVVGWELTDRVAFMASHYHDIALKKPVESQLSVNYNTCCWSANVYV
ARKLTATPIGSPDTINDLYYDNKFGVNFELRFGTNYSSGVRKMLKKGMIPYTEQYGIN
>Mature_778_residues
MKTRYSVLSVAMTAAFYTQYAQADLREQCLLGVPHFQGEEVTGDQTMMPIEIEADNAVINQPKDATYTGDVAIKQGNRSL
FADEVRVEQNGEQERRAFLKGSYRYQDNLIQAHGRDAAMDLGSETAELQNTEFQLVGRQGRGTAESGSFNHNKRILKNAT
FTACLPNDNAWSIEGNEMIQHIDEEYAEIWHARFKVLGMPVFYSPYLQFPIGDRRRSGLLIPNFHRSSKDGFAYSQPFYW
NIAPNMDATITPTYYSRRGWQISPEYRYLTKLGEGIVAGEYIGKDRLDEYRPDDNDRKRYLMHWRHNMSFLTGWRLYVDY
TKVSDKRYFSDFDSEYGSSTDGYATQQFKLGYYQPNYNLSISGKKFQTFDELDVGPYRVLPQIDFNYYNDELVKGGDFKL
FAQTARFENDSKLMPKAWRFHVEPTLNFPLANRYGSLNFETKLYATHYLQEKGSSKQADDMDKNVTRIIPQVKVDLQTVL
EADKQLFKGFNQTFEPRVQYVYRPYKDQSNIGSGLNQSVSFGYDSALLQSDYFSLFNDRRYSGLDRISSANLITAGGTNR
FFNEKTGVEVFNFSIGQTYYLSPSKIDDLSQNSTTKRSSSWALESNWKFHRKWNWHGAYQYDTRLNQTSLANTSLQYKPS
QDKLVQLSYRFASKDYINQNLRSNTYGQDIKQVGAVVGWELTDRVAFMASHYHDIALKKPVESQLSVNYNTCCWSANVYV
ARKLTATPIGSPDTINDLYYDNKFGVNFELRFGTNYSSGVRKMLKKGMIPYTEQYGIN

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family

Homologues:

Organism=Escherichia coli, GI1786239, Length=797, Percent_Identity=42.7854454203262, Blast_Score=629, Evalue=0.0,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LPTD_ACTP2 (A3N0X0)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053661.1
- ProteinModelPortal:   A3N0X0
- STRING:   A3N0X0
- GeneID:   4849645
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0962
- eggNOG:   COG1452
- HOGENOM:   HBG563511
- OMA:   TPYYWNI
- PhylomeDB:   A3N0X0
- ProtClustDB:   PRK03761
- BioCyc:   APLE416269:APL_0962-MONOMER
- HAMAP:   MF_01411
- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543

Pfam domain/function: PF03968 OstA; PF04453 OstA_C

EC number: NA

Molecular weight: Translated: 89824; Mature: 89824

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRYSVLSVAMTAAFYTQYAQADLREQCLLGVPHFQGEEVTGDQTMMPIEIEADNAVIN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCEEC
QPKDATYTGDVAIKQGNRSLFADEVRVEQNGEQERRAFLKGSYRYQDNLIQAHGRDAAMD
CCCCCEEECCEEEECCCCCEEHHHEEECCCCCHHHHHHHCCCCEECCCHHHHCCCCHHHH
LGSETAELQNTEFQLVGRQGRGTAESGSFNHNKRILKNATFTACLPNDNAWSIEGNEMIQ
CCCCHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCEEEEECCCCCEEEECHHHHHH
HIDEEYAEIWHARFKVLGMPVFYSPYLQFPIGDRRRSGLLIPNFHRSSKDGFAYSQPFYW
HHHHHHHHHHHHHHHHCCCCEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCEECCCEEE
NIAPNMDATITPTYYSRRGWQISPEYRYLTKLGEGIVAGEYIGKDRLDEYRPDDNDRKRY
EECCCCCCEECCCCCCCCCCEECCCHHHHHHHCCCCEECHHCCCHHHHHCCCCCCCHHHH
LMHWRHNMSFLTGWRLYVDYTKVSDKRYFSDFDSEYGSSTDGYATQQFKLGYYQPNYNLS
HHHHHCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCEE
ISGKKFQTFDELDVGPYRVLPQIDFNYYNDELVKGGDFKLFAQTARFENDSKLMPKAWRF
ECCCCCCCHHCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEE
HVEPTLNFPLANRYGSLNFETKLYATHYLQEKGSSKQADDMDKNVTRIIPQVKVDLQTVL
EECCEECCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCHHHCHHHHHHHHHHHHHHHHH
EADKQLFKGFNQTFEPRVQYVYRPYKDQSNIGSGLNQSVSFGYDSALLQSDYFSLFNDRR
HHHHHHHHHCCCCCCCHHHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
YSGLDRISSANLITAGGTNRFFNEKTGVEVFNFSIGQTYYLSPSKIDDLSQNSTTKRSSS
CCCHHHCCCCCEEEECCCCCCCCCCCCCEEEEEECCCEEEECCCHHCCCCCCCCCCCCCC
WALESNWKFHRKWNWHGAYQYDTRLNQTSLANTSLQYKPSQDKLVQLSYRFASKDYINQN
EEECCCCEEEEEECCCCCEEECCCCCCCCCCCCEEEECCCCCCEEEEEEHHHCHHHHHHH
LRSNTYGQDIKQVGAVVGWELTDRVAFMASHYHDIALKKPVESQLSVNYNTCCWSANVYV
HCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCHHHCCCCCEEEEECCEEE
ARKLTATPIGSPDTINDLYYDNKFGVNFELRFGTNYSSGVRKMLKKGMIPYTEQYGIN
EEEEEECCCCCCCCCHHHEECCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure
MKTRYSVLSVAMTAAFYTQYAQADLREQCLLGVPHFQGEEVTGDQTMMPIEIEADNAVIN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCEEC
QPKDATYTGDVAIKQGNRSLFADEVRVEQNGEQERRAFLKGSYRYQDNLIQAHGRDAAMD
CCCCCEEECCEEEECCCCCEEHHHEEECCCCCHHHHHHHCCCCEECCCHHHHCCCCHHHH
LGSETAELQNTEFQLVGRQGRGTAESGSFNHNKRILKNATFTACLPNDNAWSIEGNEMIQ
CCCCHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCEEEEECCCCCEEEECHHHHHH
HIDEEYAEIWHARFKVLGMPVFYSPYLQFPIGDRRRSGLLIPNFHRSSKDGFAYSQPFYW
HHHHHHHHHHHHHHHHCCCCEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCEECCCEEE
NIAPNMDATITPTYYSRRGWQISPEYRYLTKLGEGIVAGEYIGKDRLDEYRPDDNDRKRY
EECCCCCCEECCCCCCCCCCEECCCHHHHHHHCCCCEECHHCCCHHHHHCCCCCCCHHHH
LMHWRHNMSFLTGWRLYVDYTKVSDKRYFSDFDSEYGSSTDGYATQQFKLGYYQPNYNLS
HHHHHCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCEE
ISGKKFQTFDELDVGPYRVLPQIDFNYYNDELVKGGDFKLFAQTARFENDSKLMPKAWRF
ECCCCCCCHHCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEE
HVEPTLNFPLANRYGSLNFETKLYATHYLQEKGSSKQADDMDKNVTRIIPQVKVDLQTVL
EECCEECCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCHHHCHHHHHHHHHHHHHHHHH
EADKQLFKGFNQTFEPRVQYVYRPYKDQSNIGSGLNQSVSFGYDSALLQSDYFSLFNDRR
HHHHHHHHHCCCCCCCHHHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
YSGLDRISSANLITAGGTNRFFNEKTGVEVFNFSIGQTYYLSPSKIDDLSQNSTTKRSSS
CCCHHHCCCCCEEEECCCCCCCCCCCCCEEEEEECCCEEEECCCHHCCCCCCCCCCCCCC
WALESNWKFHRKWNWHGAYQYDTRLNQTSLANTSLQYKPSQDKLVQLSYRFASKDYINQN
EEECCCCEEEEEECCCCCEEECCCCCCCCCCCCEEEECCCCCCEEEEEEHHHCHHHHHHH
LRSNTYGQDIKQVGAVVGWELTDRVAFMASHYHDIALKKPVESQLSVNYNTCCWSANVYV
HCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCHHHCCCCCEEEEECCEEE
ARKLTATPIGSPDTINDLYYDNKFGVNFELRFGTNYSSGVRKMLKKGMIPYTEQYGIN
EEEEEECCCCCCCCCHHHEECCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA