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The map label for this gene is iscA [H]

Identifier: 126208405

GI number: 126208405

Start: 1070849

End: 1071172

Strand: Reverse

Name: iscA [H]

Synonym: APL_0929

Alternate gene names: 126208405

Gene position: 1071172-1070849 (Counterclockwise)

Preceding gene: 126208406

Following gene: 126208404

Centisome position: 47.1

GC content: 39.2

Gene sequence:

>324_bases
ATGGCAATTACATTAACCGAAACAGCAGCAAATCGAGTTCGTACATTCTTAGAAAACCGTGGTAAAGGGATCGGTTTACG
TTTAGGTGTGAAAACGTCGGGCTGTTCGGGACTGGCTTATGTGCTTGAGTTTGTTGATGTGTTAAATGAGGATGATCATG
TCTTTGAACAACACGGCGTAAAAGTGATTGTAGATGAAAAAAGCCTGACTTATTTAGACGGTACCGAATTGGATTTCGTC
AAAGAAGGTTTAAACGAAGGTTTTAAATATAACAATCCGAATGTGAAAGATTCTTGCGGCTGTGGTGAAAGTTTTAACGT
ATAG

Upstream 100 bases:

>100_bases
TTGTTTTTTCGCAAAATTTTGCAAAATCAACTTTTTCGTGTATTTCCGTGTTTTCTGTGGTAAAGATCGCTTTCATTTAA
CAGATTAAAAAGGATAACTT

Downstream 100 bases:

>100_bases
AGATAAACAATGAATAATCCTTTTGCACTATTTGATTTACCGGTTCAATTTGAATTGGATAACGCGCTACTTTCCGAACG
CTATTTGGCATTGCAAAGAC

Product: iron-binding protein IscA

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MAITLTETAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEFVDVLNEDDHVFEQHGVKVIVDEKSLTYLDGTELDFV
KEGLNEGFKYNNPNVKDSCGCGESFNV

Sequences:

>Translated_107_residues
MAITLTETAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEFVDVLNEDDHVFEQHGVKVIVDEKSLTYLDGTELDFV
KEGLNEGFKYNNPNVKDSCGCGESFNV
>Mature_106_residues
AITLTETAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEFVDVLNEDDHVFEQHGVKVIVDEKSLTYLDGTELDFVK
EGLNEGFKYNNPNVKDSCGCGESFNV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=42.4528301886792, Blast_Score=107, Evalue=2e-24,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=43.3734939759036, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=74.7663551401869, Blast_Score=166, Evalue=2e-43,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=46.6666666666667, Blast_Score=112, Evalue=7e-27,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=39.0909090909091, Blast_Score=79, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=40.5660377358491, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=40.5660377358491, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=36.7924528301887, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=39.0476190476191, Blast_Score=87, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=44.3396226415094, Blast_Score=105, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24649697, Length=108, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11748; Mature: 11617

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITLTETAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEFVDVLNEDDHVFEQHGV
CEEEECHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCC
KVIVDEKSLTYLDGTELDFVKEGLNEGFKYNNPNVKDSCGCGESFNV
EEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AITLTETAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEFVDVLNEDDHVFEQHGV
EEEECHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCC
KVIVDEKSLTYLDGTELDFVKEGLNEGFKYNNPNVKDSCGCGESFNV
EEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA