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The map label for this gene is dapA

Identifier: 126208375

GI number: 126208375

Start: 1042413

End: 1043300

Strand: Reverse

Name: dapA

Synonym: APL_0899

Alternate gene names: 126208375

Gene position: 1043300-1042413 (Counterclockwise)

Preceding gene: 126208376

Following gene: 126208367

Centisome position: 45.87

GC content: 42.68

Gene sequence:

>888_bases
ATGGCAACCCCACTATTTCACGGCAGCATTGTTGCACTTGTCACGCCGATGACTCACGGTGAAGTAAATTACGAAGAGTT
AAAAAAATTGGTTGAATACCATGTTCAAGCCGGTACTCACGGTATTGTATCGGTCGGTACGACAGGCGAATCGACCACAT
TAAGCATTGATGAAAATGTGAAAGTGATCAAGAAAACCGTGGAATTTGCAGATGGTCGTATTCCGATTATTGCCGGTACG
GGGTCGAATGCAACCAGCGAAGCGATTATTTTAACCAAATTATTAACCAATAGTGGCGTTGCAGGTTGTTTAAGTGTGGT
TCCTTATTACAATAAACCGACTCAAGAGGGAATGTATTTACACTATAAAGCGATTGCCGAAAGTACCGACTTACCGCAAA
TTTTATATAACGTACCGAGCCGTACCGGCAGTGATTTAAAACCGGAAACCATCGGACGTTTAGCCGAGATCCCGAATATT
GTCGGTGTAAAAGAAGCAACGGGTGATTTAACTCGTTTGCCGTTAATTAAAAAATTAGCCGGTGAAGATTTTATTTTCTT
AAGCGGTGACGATGCGACAGGCTTAGAATCAATGAAACTTGGCGGTCAAGGCGTAATTTCGGTAACCAATAACGTTGCGG
CGGCGGATATGGCAAAAATGTGCGAGCTTGCATTAGCCGGTAAATTTGATGAAGCGGAAGCGATTAATCAGCGTTTAATG
GCATTGCACCACGACTTATTTATTGAAGCGAATCCGATTCCGGTCAAATGGGCTGCTTATAAATTAGGTTTAATCAGTGA
GCCGAATTTACGTTTACCATTAACCACGTTATCGGAATCCGCTCAACCTACCGTGTTAGCGGCATTGCAAAAAGCCGGTT
TAATTTAA

Upstream 100 bases:

>100_bases
AATTTTTTAGTTGTTATTCGCAAAAAATACTTGCATATTTAAAGCGAATTTTGCAATCTAACAAAATATTGTACATAACA
AAAAAATACAGGAGATGATT

Downstream 100 bases:

>100_bases
CCGCTTGTTATTAGCTATAGATAAAAACCCCGTACTTGAATACGGGGTTTTATATTAGACAAGCGGTCAAATTTACTAAA
AAATTTGCAATACTACTTTA

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MATPLFHGSIVALVTPMTHGEVNYEELKKLVEYHVQAGTHGIVSVGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGT
GSNATSEAIILTKLLTNSGVAGCLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPSRTGSDLKPETIGRLAEIPNI
VGVKEATGDLTRLPLIKKLAGEDFIFLSGDDATGLESMKLGGQGVISVTNNVAAADMAKMCELALAGKFDEAEAINQRLM
ALHHDLFIEANPIPVKWAAYKLGLISEPNLRLPLTTLSESAQPTVLAALQKAGLI

Sequences:

>Translated_295_residues
MATPLFHGSIVALVTPMTHGEVNYEELKKLVEYHVQAGTHGIVSVGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGT
GSNATSEAIILTKLLTNSGVAGCLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPSRTGSDLKPETIGRLAEIPNI
VGVKEATGDLTRLPLIKKLAGEDFIFLSGDDATGLESMKLGGQGVISVTNNVAAADMAKMCELALAGKFDEAEAINQRLM
ALHHDLFIEANPIPVKWAAYKLGLISEPNLRLPLTTLSESAQPTVLAALQKAGLI
>Mature_294_residues
ATPLFHGSIVALVTPMTHGEVNYEELKKLVEYHVQAGTHGIVSVGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGTG
SNATSEAIILTKLLTNSGVAGCLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPSRTGSDLKPETIGRLAEIPNIV
GVKEATGDLTRLPLIKKLAGEDFIFLSGDDATGLESMKLGGQGVISVTNNVAAADMAKMCELALAGKFDEAEAINQRLMA
LHHDLFIEANPIPVKWAAYKLGLISEPNLRLPLTTLSESAQPTVLAALQKAGLI

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=236, Percent_Identity=29.6610169491525, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI13540533, Length=224, Percent_Identity=27.2321428571429, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788823, Length=292, Percent_Identity=63.3561643835616, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI87082415, Length=281, Percent_Identity=29.5373665480427, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789620, Length=294, Percent_Identity=27.2108843537415, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786463, Length=243, Percent_Identity=28.3950617283951, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_ACTP2 (A3N0Q9)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053600.1
- ProteinModelPortal:   A3N0Q9
- SMR:   A3N0Q9
- STRING:   A3N0Q9
- GeneID:   4848494
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0899
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   GMYRHFK
- PhylomeDB:   A3N0Q9
- ProtClustDB:   PRK03170
- BioCyc:   APLE416269:APL_0899-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31420; Mature: 31288

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 164-164 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPLFHGSIVALVTPMTHGEVNYEELKKLVEYHVQAGTHGIVSVGTTGESTTLSIDENV
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCH
KVIKKTVEFADGRIPIIAGTGSNATSEAIILTKLLTNSGVAGCLSVVPYYNKPTQEGMYL
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEE
HYKAIAESTDLPQILYNVPSRTGSDLKPETIGRLAEIPNIVGVKEATGDLTRLPLIKKLA
EEEEHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHC
GEDFIFLSGDDATGLESMKLGGQGVISVTNNVAAADMAKMCELALAGKFDEAEAINQRLM
CCCEEEEECCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ALHHDLFIEANPIPVKWAAYKLGLISEPNLRLPLTTLSESAQPTVLAALQKAGLI
HHHHHEEEECCCCCEEEEEEEECCCCCCCCEEEHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ATPLFHGSIVALVTPMTHGEVNYEELKKLVEYHVQAGTHGIVSVGTTGESTTLSIDENV
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCH
KVIKKTVEFADGRIPIIAGTGSNATSEAIILTKLLTNSGVAGCLSVVPYYNKPTQEGMYL
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEE
HYKAIAESTDLPQILYNVPSRTGSDLKPETIGRLAEIPNIVGVKEATGDLTRLPLIKKLA
EEEEHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHC
GEDFIFLSGDDATGLESMKLGGQGVISVTNNVAAADMAKMCELALAGKFDEAEAINQRLM
CCCEEEEECCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ALHHDLFIEANPIPVKWAAYKLGLISEPNLRLPLTTLSESAQPTVLAALQKAGLI
HHHHHEEEECCCCCEEEEEEEECCCCCCCCEEEHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA