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The map label for this gene is folA [H]

Identifier: 126208359

GI number: 126208359

Start: 1023046

End: 1023534

Strand: Reverse

Name: folA [H]

Synonym: APL_0883

Alternate gene names: 126208359

Gene position: 1023534-1023046 (Counterclockwise)

Preceding gene: 126208366

Following gene: 126208358

Centisome position: 45.0

GC content: 41.72

Gene sequence:

>489_bases
ATGAAGATAAGTTTAATTGTCGCCAGAACCTTAAATCATGTGATTGGTAAAGAGAATCAAATGCCATGGAATTTACCGGT
GGATTTGGCTTGGTTTCGACAAAATACGCTAGGTAAGCCGGTGATTATGGGGCGCAAAACCTATGAATCTATCGGACGAC
TTTTACCGAAACGACCGAATATTATTTTGTCACGTTCCGGCTTTTGTGTGGAAGGGGCTTATACGGCGCAAAATTTTGCA
CAAGCGGCCGAATTAGCCGAAACTTTTGCAAATACGGATGAAATTATGGTAATCGGCGGCGGTGAGTTATTTAAACAAAC
GATTCCGCAAGCGGACAGACTTTATTTAACGGAAATTCAAGCCGAAATCGAGGGCGATACGCTATTTGAGTTTGACGAAT
CGAATTGGACGTTAGTGGAAGAAAAATGGTCGGAAATTGACGAGCAAAACGCCTATCGTTGCCGCTTTATGATTTTAGAG
AGAAAATAA

Upstream 100 bases:

>100_bases
ATTGTCTTTTGCATAGTTTTATCCCGATAAAAAATTTAGCACCAAAGTATCACTTTTATTTGTTAAAATCACGCCTAATT
TTGTTGAAAAGGAAGAAAAA

Downstream 100 bases:

>100_bases
AACGCTTATTTTATAAGCAAACAATTAATCTTTACAAAACGCAAACCTTTGCATTTCTATTGCTTAAACACAAAATAAAA
AAGAAAGCGTACAATCGGTT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MKISLIVARTLNHVIGKENQMPWNLPVDLAWFRQNTLGKPVIMGRKTYESIGRLLPKRPNIILSRSGFCVEGAYTAQNFA
QAAELAETFANTDEIMVIGGGELFKQTIPQADRLYLTEIQAEIEGDTLFEFDESNWTLVEEKWSEIDEQNAYRCRFMILE
RK

Sequences:

>Translated_162_residues
MKISLIVARTLNHVIGKENQMPWNLPVDLAWFRQNTLGKPVIMGRKTYESIGRLLPKRPNIILSRSGFCVEGAYTAQNFA
QAAELAETFANTDEIMVIGGGELFKQTIPQADRLYLTEIQAEIEGDTLFEFDESNWTLVEEKWSEIDEQNAYRCRFMILE
RK
>Mature_162_residues
MKISLIVARTLNHVIGKENQMPWNLPVDLAWFRQNTLGKPVIMGRKTYESIGRLLPKRPNIILSRSGFCVEGAYTAQNFA
QAAELAETFANTDEIMVIGGGELFKQTIPQADRLYLTEIQAEIEGDTLFEFDESNWTLVEEKWSEIDEQNAYRCRFMILE
RK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=161, Percent_Identity=49.0683229813665, Blast_Score=152, Evalue=1e-38,
Organism=Drosophila melanogaster, GI116008026, Length=179, Percent_Identity=27.9329608938547, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24647458, Length=179, Percent_Identity=27.9329608938547, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18633; Mature: 18633

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISLIVARTLNHVIGKENQMPWNLPVDLAWFRQNTLGKPVIMGRKTYESIGRLLPKRPN
CEEEEEHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEECCHHHHHHHHHCCCCCC
IILSRSGFCVEGAYTAQNFAQAAELAETFANTDEIMVIGGGELFKQTIPQADRLYLTEIQ
EEEECCCCEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCEEEEEEE
AEIEGDTLFEFDESNWTLVEEKWSEIDEQNAYRCRFMILERK
EECCCCEEEEECCCCCEEEHHHHHHCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MKISLIVARTLNHVIGKENQMPWNLPVDLAWFRQNTLGKPVIMGRKTYESIGRLLPKRPN
CEEEEEHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEECCHHHHHHHHHCCCCCC
IILSRSGFCVEGAYTAQNFAQAAELAETFANTDEIMVIGGGELFKQTIPQADRLYLTEIQ
EEEECCCCEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCEEEEEEE
AEIEGDTLFEFDESNWTLVEEKWSEIDEQNAYRCRFMILERK
EECCCCEEEEECCCCCEEEHHHHHHCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]