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The map label for this gene is apfA [H]

Identifier: 126208356

GI number: 126208356

Start: 1020635

End: 1021081

Strand: Reverse

Name: apfA [H]

Synonym: APL_0880

Alternate gene names: 126208356

Gene position: 1021081-1020635 (Counterclockwise)

Preceding gene: 126208358

Following gene: 126208355

Centisome position: 44.89

GC content: 41.83

Gene sequence:

>447_bases
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACGGTAACGGGGAA
GGGGAATCTGCAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGTGCGATAAATTAG

Upstream 100 bases:

>100_bases
GCGATACGGATCGCAGAAGTCGGTCATCTTACTGGTTAGCGAAAACAAATTATTGCAGACTGTTTAGTAAGCAGATCTTA
GATATGATAAGGAGAGGGAT

Downstream 100 bases:

>100_bases
AGTCTTCGGAGCAAGATATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTCCGGATTTCCGAGGAACAGTGG
CAAAAAAATTATGATGAACG

Product: type 4 prepilin subunit ApfA precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCAIN

Sequences:

>Translated_148_residues
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCAIN
>Mature_148_residues
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCAIN

Specific function: Not Yet Known. [C]

COG id: COG4969

COG function: function code NU; Tfp pilus assembly protein, major pilin PilA

Gene ontology:

Cell location: Fimbrium [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-Me-Phe pilin family [H]

Homologues:

Organism=Escherichia coli, GI1786297, Length=123, Percent_Identity=41.4634146341463, Blast_Score=93, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012902
- InterPro:   IPR001120 [H]

Pfam domain/function: PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 16007; Mature: 16007

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCE
EICIYNTGDSKNCSGGQNGVRKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPI
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCEEEEEECCCHHHCCCEECEE
HNGSTISWETKCKGEDLSLFPANFCAIN
CCCCEEEEECCCCCCCCEECCCCEEECC
>Mature Secondary Structure
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCE
EICIYNTGDSKNCSGGQNGVRKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPI
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCEEEEEECCCHHHCCCEECEE
HNGSTISWETKCKGEDLSLFPANFCAIN
CCCCEEEEECCCCCCCCEECCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]