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The map label for this gene is apfB [H]

Identifier: 126208355

GI number: 126208355

Start: 1019223

End: 1020617

Strand: Reverse

Name: apfB [H]

Synonym: APL_0879

Alternate gene names: 126208355

Gene position: 1020617-1019223 (Counterclockwise)

Preceding gene: 126208356

Following gene: 126208354

Centisome position: 44.87

GC content: 40.14

Gene sequence:

>1395_bases
ATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTCCGGATTTCCGAGGAACAGTGGCAAAAAAATTATGATGA
ACGTCATATCTTATTACGTTATCTCACCGTACCGCTTCGGGAAGACCAACAAACATTATGGTTGGCAATCGATGATATGA
ATAATCTCAGTGCTTGCGAGATTTTTGCTTTTCTTACGCATAAGCGAATTGAGCCGGTATTGGTCTCTTCCGAAGAATTG
AAATACCTGCTCGGGCAATTAATGCCGGAACAACAATCGGTATATGACGAAACGGAATTACCTTATCAACCTTTAGCACA
AGCGCAGCATTCGGATGTTAATGATCCGATTATTCAAGTGTTGGACGGCCTGTTTAAATACGGTTTAAAACATAATGCTT
CGGATATTCATTTTGAACCGCAAAAAGACAAAATGTTGATTCGATTACGTATCGACGGCGTGTTACATCCGTATAAATCA
TTTTCTCTCCAACTTGCCAATCGACTTATTTCTCGCATTAAATTATTAGCTAAACTGGATATTAGTGAATCCCGTCTTCC
GCAAGACGGGCAATTCGGTTTTAAGACAATCTTATCCGAAACATTGGATTTCCGTGTTTCGAGTTTACCGACTTATTGGG
GCGAAAAAGTCGTTTTACGGCTACAAAAAAATAAACCGACGCATTTTGATTTTACTACGTTGGGCTTCTTGGCGGAGCAG
AAGGAAAAGTTGTTGCGGGCATTGCGGCAGCCTCAAGGATTAATATTAGTTACGGGACCGACCGGCAGTGGTAAAAGTAT
TACCTTATATAGTGCGTTGGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCCGAAGATCCGATAGAAATCGAAC
TTGAAGGCATAATCCAAACGCAAATAAACCCAGCGATAGATTTAGGGTTCAGCCGGCTATTAAGAACGTTTTTACGACAA
GATCCGGATGTGATTATGTTAGGTGAAATTCGTGATCAAGAAAGTGCCGAAATTGCATTAAGTGCGGCACAAACCGGGCA
TTTAGTGCTTTCGACCTTACATACCAATGATGCGCCTTCGGCAATCGAACGCTTATTGCAGCTCGGTATCAAAGAATATG
AAATCAATAATTCGTTATTACTTGTCGTGGCGCAACGTTTAGTTCGTAAATTATGTACCGAATGCGGCGGAAAAGGCTGT
AAATATTGTTATCACGGCTACAGAGGCAGAATCGGCGTTTATCAACTGTTTTCAAAAAATGGAAAAATTTTTGAGAAATC
GACCGCTTGTTTGGATTTTGAAAACTTATATCAAAGTGCGCAACAGAAATTCAAGGCGGGAATCACTTCATTAGAAGAAA
TCGAGAGAGTGTTAGGTCATGTTGAAAGTGTATGA

Upstream 100 bases:

>100_bases
ACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAGGGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGTGCGATAAAT
TAGAGTCTTCGGAGCAAGAT

Downstream 100 bases:

>100_bases
ATTTCATTGGAAAGCTAAAAATCGTTTTCAACAACTTCAGAAAGGAAAAGCGCTAGCTTGTCATCGAACGGAATTGGAAC
GGCGTTTATTAGCAAAAGGC

Product: fimbrial biogenesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHFEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKAGITSLEEIERVLGHVESV

Sequences:

>Translated_464_residues
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHFEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKAGITSLEEIERVLGHVESV
>Mature_464_residues
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHFEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKAGITSLEEIERVLGHVESV

Specific function: Unknown

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=464, Percent_Identity=40.5172413793103, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1789723, Length=385, Percent_Identity=41.8181818181818, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI87082188, Length=254, Percent_Identity=37.007874015748, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 53028; Mature: 53028

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACE
CCCEEECCCCCEEEEECHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHH
IFAFLTHKRIEPVLVSSEELKYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQV
HHHHHHHCCCCCEEECHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHCCCCCCHHHHH
LDGLFKYGLKHNASDIHFEPQKDKMLIRLRIDGVLHPYKSFSLQLANRLISRIKLLAKLD
HHHHHHHCCCCCCCCCEECCCCCCEEEEEEECCHHCCHHHHHHHHHHHHHHHHHHHHHCC
ISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
CCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHH
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQT
HHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHHCCCCCEEEECCCCCEEEEEEEEEE
QINPAIDLGFSRLLRTFLRQDPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPS
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHEEEECCCCCCEEEEEECCCCCHH
AIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGCKYCYHGYRGRIGVYQLFSKN
HHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHCCC
GKIFEKSTACLDFENLYQSAQQKFKAGITSLEEIERVLGHVESV
CCEEECCHHEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACE
CCCEEECCCCCEEEEECHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHH
IFAFLTHKRIEPVLVSSEELKYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQV
HHHHHHHCCCCCEEECHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHCCCCCCHHHHH
LDGLFKYGLKHNASDIHFEPQKDKMLIRLRIDGVLHPYKSFSLQLANRLISRIKLLAKLD
HHHHHHHCCCCCCCCCEECCCCCCEEEEEEECCHHCCHHHHHHHHHHHHHHHHHHHHHCC
ISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
CCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHH
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQT
HHHHHHHHCCCCCEEEEECCCCCCCEEEHHHHHHHHCCCCCEEEECCCCCEEEEEEEEEE
QINPAIDLGFSRLLRTFLRQDPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPS
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHEEEECCCCCCEEEEEECCCCCHH
AIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGCKYCYHGYRGRIGVYQLFSKN
HHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHCCC
GKIFEKSTACLDFENLYQSAQQKFKAGITSLEEIERVLGHVESV
CCEEECCHHEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]