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The map label for this gene is queF

Identifier: 126208320

GI number: 126208320

Start: 980048

End: 980887

Strand: Reverse

Name: queF

Synonym: APL_0844

Alternate gene names: 126208320

Gene position: 980887-980048 (Counterclockwise)

Preceding gene: 126208321

Following gene: 126208318

Centisome position: 43.13

GC content: 40.36

Gene sequence:

>840_bases
ATGAACTACAACGATAAATCATTATCTGCTCTGAAATTAGGGCAAAAAACCGAATATAAAAGTGAATATGATCCTACGCT
TTTACAGCCTGTTCCCCGTAAATTAAACCGTGACGGTTTGGGGATTACCGAACAACAACCGTTTGATCGAGGTGCGGATG
TTTGGACTTGTTATGAACTGTCTTGGCTAAATGAAAATGGTTTACCACAGGTGGCGATAGCGGATGTGGCGATTGATTTT
CGTAGCGAGAATTTAATTGAATCAAAAAGTTTTAAACTCTATCTAAACAGCTTTAACCAAACCAAATTTGCCTCATTGGA
ACAAGTGGAACAAACATTAGCCAAAGATCTCAGCCAATGTGCAAGCGGTCAAGTTTCGGTAAAAGTTTACAAATTATCCG
CTTACACCCAGCAACCGATTGTGGATTTTGCCGGTGAATGTATTGATGAACAAGATATTCAAATTGACAGTTACGAATTT
TCTAACGAGCATTTAGCAAGCGTGGCAGAAGGCGAAGTGGTTGAAGAGACCTTAGTCAGCCATTTGCTGAAATCAAACTG
TTTGATTACCTCGCAACCGGACTGGGGTAGCGTACAAATTCACTATGTCGGCAAAAAATTAAATCGAGAAAAATTATTAC
GCTATTTAGTGTCATTCCGTGAACATAATGAGTTCCACGAGCAATGCGTAGAGCGTATCTTTATCGATTTGATTCAATTT
ACACAGCCGGAAAAACTGACGGTCTATGCACGTTATACCCGCCGAGGCGGTTTGGATATCAACCCGTTCCGTTCAAATTT
TGAGTCTGTTCCGCAAAATTTGAGAATGGCAAGGCAGTAA

Upstream 100 bases:

>100_bases
AACAGGCAAACCTGTTACCGCAGAAGGCGAAGCGAAAGCCGATTTGGTATTAGACAGCTTAGTGGATTTAGTCCGTTATA
TTAAATAATAAAGAAAGAAG

Downstream 100 bases:

>100_bases
GAAAAATACCCCAGAAAATCGAGAACGATATTTTGGGGCGTTTTTTTATTTATTTTCTATTTGCTTTTCAACCGAAGTCA
GCATTCCTCTTAATAAATTC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNYNDKSLSALKLGQKTEYKSEYDPTLLQPVPRKLNRDGLGITEQQPFDRGADVWTCYELSWLNENGLPQVAIADVAIDF
RSENLIESKSFKLYLNSFNQTKFASLEQVEQTLAKDLSQCASGQVSVKVYKLSAYTQQPIVDFAGECIDEQDIQIDSYEF
SNEHLASVAEGEVVEETLVSHLLKSNCLITSQPDWGSVQIHYVGKKLNREKLLRYLVSFREHNEFHEQCVERIFIDLIQF
TQPEKLTVYARYTRRGGLDINPFRSNFESVPQNLRMARQ

Sequences:

>Translated_279_residues
MNYNDKSLSALKLGQKTEYKSEYDPTLLQPVPRKLNRDGLGITEQQPFDRGADVWTCYELSWLNENGLPQVAIADVAIDF
RSENLIESKSFKLYLNSFNQTKFASLEQVEQTLAKDLSQCASGQVSVKVYKLSAYTQQPIVDFAGECIDEQDIQIDSYEF
SNEHLASVAEGEVVEETLVSHLLKSNCLITSQPDWGSVQIHYVGKKLNREKLLRYLVSFREHNEFHEQCVERIFIDLIQF
TQPEKLTVYARYTRRGGLDINPFRSNFESVPQNLRMARQ
>Mature_279_residues
MNYNDKSLSALKLGQKTEYKSEYDPTLLQPVPRKLNRDGLGITEQQPFDRGADVWTCYELSWLNENGLPQVAIADVAIDF
RSENLIESKSFKLYLNSFNQTKFASLEQVEQTLAKDLSQCASGQVSVKVYKLSAYTQQPIVDFAGECIDEQDIQIDSYEF
SNEHLASVAEGEVVEETLVSHLLKSNCLITSQPDWGSVQIHYVGKKLNREKLLRYLVSFREHNEFHEQCVERIFIDLIQF
TQPEKLTVYARYTRRGGLDINPFRSNFESVPQNLRMARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=278, Percent_Identity=64.3884892086331, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_ACTP2 (A3N0K4)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053545.1
- ProteinModelPortal:   A3N0K4
- SMR:   A3N0K4
- STRING:   A3N0K4
- GeneID:   4850362
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0844
- eggNOG:   COG0780
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- PhylomeDB:   A3N0K4
- ProtClustDB:   PRK11792
- BioCyc:   APLE416269:APL_0844-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 32106; Mature: 32106

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYNDKSLSALKLGQKTEYKSEYDPTLLQPVPRKLNRDGLGITEQQPFDRGADVWTCYEL
CCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEE
SWLNENGLPQVAIADVAIDFRSENLIESKSFKLYLNSFNQTKFASLEQVEQTLAKDLSQC
EECCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ASGQVSVKVYKLSAYTQQPIVDFAGECIDEQDIQIDSYEFSNEHLASVAEGEVVEETLVS
HCCCEEEEEEEEECCCCCCHHHHHHHHCCCCCCEEECEECCCHHHHHHHCCHHHHHHHHH
HLLKSNCLITSQPDWGSVQIHYVGKKLNREKLLRYLVSFREHNEFHEQCVERIFIDLIQF
HHHHCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
TQPEKLTVYARYTRRGGLDINPFRSNFESVPQNLRMARQ
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNYNDKSLSALKLGQKTEYKSEYDPTLLQPVPRKLNRDGLGITEQQPFDRGADVWTCYEL
CCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEE
SWLNENGLPQVAIADVAIDFRSENLIESKSFKLYLNSFNQTKFASLEQVEQTLAKDLSQC
EECCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ASGQVSVKVYKLSAYTQQPIVDFAGECIDEQDIQIDSYEFSNEHLASVAEGEVVEETLVS
HCCCEEEEEEEEECCCCCCHHHHHHHHCCCCCCEEECEECCCHHHHHHHCCHHHHHHHHH
HLLKSNCLITSQPDWGSVQIHYVGKKLNREKLLRYLVSFREHNEFHEQCVERIFIDLIQF
HHHHCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
TQPEKLTVYARYTRRGGLDINPFRSNFESVPQNLRMARQ
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA