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The map label for this gene is purB [H]

Identifier: 126208300

GI number: 126208300

Start: 952229

End: 953596

Strand: Reverse

Name: purB [H]

Synonym: APL_0824

Alternate gene names: 126208300

Gene position: 953596-952229 (Counterclockwise)

Preceding gene: 126208301

Following gene: 126208299

Centisome position: 41.93

GC content: 42.03

Gene sequence:

>1368_bases
ATGGAACTTACCGCTTTAACAGCATTATCCCCAATCGATGGTCGTTACCAAGACAAAGCTGCCGCATTACGCCCGATTTT
TAGTGAATTCGGTTTACTAAAATTCCGTGTGACGGTAGAAGTTCGTTGGTTACAAAAATTGGCTTCTCAGGCACAGCTCA
AAGAAGTTCCAGCTTTTTCTGAAAAAGCAAACGATTACCTAAATCAAATTGTAGCAAATTTCTCGTTAGAAGATGCAAAT
CGTATCAAAACGATTGAACGCACTACCAACCACGACGTAAAAGCGGTGGAATATTTCTTAAAAGAAAAATGTGAAGCGTT
ACCCGAATTACAAGCGGTAAATGAATTTATTCATTTTGCTTGTACTTCGGAAGATATTAACAATACTTCACACGCATTAA
TGTTAAAAACGGCACGTGAAGAAGTATTATTGCCGGAATGGAAAAAAATGATTGATGCGGTGGTGGAATTAGCAAAACGT
TATCAACATATTCCACTACTTTCTCGTACGCACGGTCAGCCGGCAAGCCCGACCACTATCGGTAAAGAGATGGCAAACGT
GGCATATCGTCTTAAACGCCAATACAAACAGCTTGAAAACTTAGAGATCTTAGCGAAAATCAACGGTGCGGTGGGTAACT
ACAATGCGCATTTATCCGCTTATCCTGATGTGGATTGGCATACTTTCAGCCAAGAGTTTGTAGAATCGTTAGGCGTAACT
TGGAACCCGTACACTACACAAATTGAACCGCACGATTACATTGCGGAATTCTTTGATTGTGTGGCACGTTTTAATACGAT
CGTGATCGATTTTGACCGTGATATGTGGGGCTATATTGCATTAAATCACTTCAAACAACGCACGATTGCCGGTGAAATAG
GTTCGTCCACCATGCCGCATAAAGTGAACCCGATCGACTTTGAAAACTCGGAAGGTAACCTTGGCTTAGCGAATGCGGTA
ATGAACCATTTGGGTCAAAAACTACCGATTTCTCGTTGGCAGCGCGACTTAACCGACTCAACCGTATTACGTAATTTAGG
CGTGGGTTTAGGTTATTGCTTAATCGCTTATGCGGCAACCTTAAAAGGCGTAAGTAAGTTGGAAGTAAACGAGCAACATT
TACGTGACGAGTTAAATCAAAACTGGGAAGTGTTGGCGGAGCCGATTCAAACGGTAATGCGTCGTTACGGTATCGAAAAA
CCATACGAGAAACTGAAAGAATTAACGCGCGGTAAACGTGTCGATGAAGCGGCAATGCGAGAGTTTATCGAGAAACTTGA
GATTCCGGCGGAAGAAAAAGTGCGCTTAAAAGAGATGACGCCGGCAAGTTATATCGGTTATGCGGTAGAACTTGTTGAAA
AATTGTAA

Upstream 100 bases:

>100_bases
GAAAGATTCTTAATCAGGCACAACAGTTTTACAAAAGTATTTAAACAAGTTCTCCGTAATGGAGAACTTGTTCCGAACAT
TTATTTATTTGGAGAAAACA

Downstream 100 bases:

>100_bases
AATTTAAGTTAAAAATGACCGCTGGTAAGCGGTCATTTTTTTGAAAACTTTGCGGAGGAATAAGTAAATGAAACAATCTA
TTCGTCACAATAAAATTATC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MELTALTALSPIDGRYQDKAAALRPIFSEFGLLKFRVTVEVRWLQKLASQAQLKEVPAFSEKANDYLNQIVANFSLEDAN
RIKTIERTTNHDVKAVEYFLKEKCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKKMIDAVVELAKR
YQHIPLLSRTHGQPASPTTIGKEMANVAYRLKRQYKQLENLEILAKINGAVGNYNAHLSAYPDVDWHTFSQEFVESLGVT
WNPYTTQIEPHDYIAEFFDCVARFNTIVIDFDRDMWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MNHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYAATLKGVSKLEVNEQHLRDELNQNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDEAAMREFIEKLEIPAEEKVRLKEMTPASYIGYAVELVEKL

Sequences:

>Translated_455_residues
MELTALTALSPIDGRYQDKAAALRPIFSEFGLLKFRVTVEVRWLQKLASQAQLKEVPAFSEKANDYLNQIVANFSLEDAN
RIKTIERTTNHDVKAVEYFLKEKCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKKMIDAVVELAKR
YQHIPLLSRTHGQPASPTTIGKEMANVAYRLKRQYKQLENLEILAKINGAVGNYNAHLSAYPDVDWHTFSQEFVESLGVT
WNPYTTQIEPHDYIAEFFDCVARFNTIVIDFDRDMWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MNHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYAATLKGVSKLEVNEQHLRDELNQNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDEAAMREFIEKLEIPAEEKVRLKEMTPASYIGYAVELVEKL
>Mature_455_residues
MELTALTALSPIDGRYQDKAAALRPIFSEFGLLKFRVTVEVRWLQKLASQAQLKEVPAFSEKANDYLNQIVANFSLEDAN
RIKTIERTTNHDVKAVEYFLKEKCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKKMIDAVVELAKR
YQHIPLLSRTHGQPASPTTIGKEMANVAYRLKRQYKQLENLEILAKINGAVGNYNAHLSAYPDVDWHTFSQEFVESLGVT
WNPYTTQIEPHDYIAEFFDCVARFNTIVIDFDRDMWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MNHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYAATLKGVSKLEVNEQHLRDELNQNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDEAAMREFIEKLEIPAEEKVRLKEMTPASYIGYAVELVEKL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=455, Percent_Identity=78.021978021978, Blast_Score=767, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 52035; Mature: 52035

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELTALTALSPIDGRYQDKAAALRPIFSEFGLLKFRVTVEVRWLQKLASQAQLKEVPAFS
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHCCCHH
EKANDYLNQIVANFSLEDANRIKTIERTTNHDVKAVEYFLKEKCEALPELQAVNEFIHFA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNTSHALMLKTAREEVLLPEWKKMIDAVVELAKRYQHIPLLSRTHGQPASPTTI
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHH
GKEMANVAYRLKRQYKQLENLEILAKINGAVGNYNAHLSAYPDVDWHTFSQEFVESLGVT
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAEFFDCVARFNTIVIDFDRDMWGYIALNHFKQRTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLANAVMNHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYAAT
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGVSKLEVNEQHLRDELNQNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDEAAMR
HCCHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
EFIEKLEIPAEEKVRLKEMTPASYIGYAVELVEKL
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MELTALTALSPIDGRYQDKAAALRPIFSEFGLLKFRVTVEVRWLQKLASQAQLKEVPAFS
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHCCCHH
EKANDYLNQIVANFSLEDANRIKTIERTTNHDVKAVEYFLKEKCEALPELQAVNEFIHFA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNTSHALMLKTAREEVLLPEWKKMIDAVVELAKRYQHIPLLSRTHGQPASPTTI
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHH
GKEMANVAYRLKRQYKQLENLEILAKINGAVGNYNAHLSAYPDVDWHTFSQEFVESLGVT
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAEFFDCVARFNTIVIDFDRDMWGYIALNHFKQRTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLANAVMNHLGQKLPISRWQRDLTDSTVLRNLGVGLGYCLIAYAAT
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGVSKLEVNEQHLRDELNQNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDEAAMR
HCCHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
EFIEKLEIPAEEKVRLKEMTPASYIGYAVELVEKL
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]