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The map label for this gene is aceF [H]

Identifier: 126208250

GI number: 126208250

Start: 885474

End: 887372

Strand: Reverse

Name: aceF [H]

Synonym: APL_0772

Alternate gene names: 126208250

Gene position: 887372-885474 (Counterclockwise)

Preceding gene: 126208251

Following gene: 126208249

Centisome position: 39.01

GC content: 44.92

Gene sequence:

>1899_bases
ATGTCAAAACAAATTAATGTGCCAGACATCGGTTCGGATGAAGTTAGCGTAACCGAAGTCATGGTAAAAGCAGGCGATAC
AATTACTGTTGATCAATCGATTATTAATGTAGAAGGCGACAAAGCTTCAATGGAAGTTCCGGCGCCGGAAGCAGGTGTTG
TTAAAGAAGTTTTAGTAAAAGTCGGCGATAAAGTTACAACCGGTTCACCAATGCTAGTATTAGAAGCGGCGGAATCTGCG
CCGGCTCTGGTAGCGGAAGCGCCTGTACAAGCCGTAGCGCCTGTGGCAACAGCATCAGCGGTTGTTGAAGTGAACGTACC
GGATATCGGTTCGGACGAAGTAAACGTAACCGAAATTATGGTTAAAGTTGGTGATTCGGTTGAAGTTGATCAATCAATCA
TTAATGTAGAAGGTGATAAAGCTTCAATGGAAGTTCCGGCACCTATCGCAGGCGTTGTAAAAGAAATTTTAATCAATGTA
GGCGATAAAGTAACAACCGGCAAATTAATTATGAAATTTGAAACGGTTTCAGCAGCGCCAGCTGCAGAGGAAGCTCCGGC
ACAAGCTGCCGCACCGGTTGCAACAGCTTCAGCAATCAAAGAAGTAAATGTACCGGATATCGGCGGTGACGAAGTAAACG
TAACCGAAATCATGGTAAACGTTGGCGATACGGTATCAGAAGAGCAATCTTTAATTACCGTTGAAGGCGACAAAGCTTCA
ATGGAAGTTCCGGCTCCGTTTGCAGGTGTGGTAAAAGAAATTTTAGTAAAATCGGGCGATAAAGTTTCAACCGGTTCATT
AATTATGAAATTTGAAGTAGCAGGTGCGGCACCGGCAGCAGCTCCTGCTCCTGCAGTACAAGCAGCTGCGCCAGCACCAG
CGGCACAACCGGCAGCGCAACCAGCTCAAAGCGGTAACGTTTCAGGTCTAAGTCAAGATGTGGTGGCAGCGGCAGGTGCT
TATGCACACGCAACACCGGTAATTCGTCGCTTAGCGCGTGAATTCGGCGTGAACTTAGATAAAGTGAAAGGCTCTGGTCG
TAAAGGTCGTATCGTTAAAGAAGATATCCAAACATACGTTAAAACGGCAGTTCAAGTATTTGAAAAACAAGGCGGAACGG
CAGCGGCTGCAACAGGTGCGGCGAATGGCGCAGGTTTAGGTTTATTACCATGGCCGAAAGTGGACTTCAGCAAATTCGGT
GAAGTTGAAGAAGTAGAATTAAGCCGTATCAATAAGATTTCAGGTGCGAACTTACACCGTAACTGGGTAATGATTCCTCA
CGTTACACACTTCGACCGTACGGATATCACAGATTTAGAAGCATTCCGTAAAGAACAAAATAAACTTGCGGAAAAACAAA
AATTAGATGTGAAGATCACACCGGTTGTATTCATTATGAAAGCGGTTGCGAAAGCGTTAGAAGCGTTCCCACGTTTCAAC
AGTTCAATCTCTGAAGATGCACAACGTTTAACCTTGAAAAAATACATCAATATCGGCGTGGCGGTAGATACTCCGAACGG
CTTAGTTGTTCCTGTATTTAAAAATGTGAACAAAAAAGGTATTGTTGAACTTTCTCGCGAGTTAATGGAAGTATCGAAGA
AAGCGCGTGACGGTAAATTAACCGCATCGGATATGCAAGGCGGATGTTTCACGATTTCAAGTATCGGCGGTTTAGGTACA
ACACACTTCACTCCAATCGTGAATGCGCCGGAAGTTGCAATTTTAGGCGTATCTAAGTCTGAAATGGCACCGGTATGGAA
CGGTAAAGAATTCGAACCGCGCTTAATGCTTCCGTTAGCGTTATCATTCGACCACCGTGTAATCGACGGTGCTGACGGTG
CAAGATTCTTAACCTACATCAATGGTGTATTAGCGGACATCCGTCGCTTAGTTATGTAA

Upstream 100 bases:

>100_bases
TCGGCTTAAACGTAGATCGTATTAACCCGTTATACGCATAAGAAAATAGCATTCTCCCCCTTATGGGGGAGAATAATTTA
TCAAATGAAGGATTACCAAA

Downstream 100 bases:

>100_bases
TAGGTAAGCGGTTGAATTTACAAAAAATGTTGTAAAATCGACCGCTTGTATCGTAGGGGGCGCAGTACTTGTGCCCCTAC
AACAAAGGAATAAATATGAG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MSKQINVPDIGSDEVSVTEVMVKAGDTITVDQSIINVEGDKASMEVPAPEAGVVKEVLVKVGDKVTTGSPMLVLEAAESA
PALVAEAPVQAVAPVATASAVVEVNVPDIGSDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPIAGVVKEILINV
GDKVTTGKLIMKFETVSAAPAAEEAPAQAAAPVATASAIKEVNVPDIGGDEVNVTEIMVNVGDTVSEEQSLITVEGDKAS
MEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAGAAPAAAPAPAVQAAAPAPAAQPAAQPAQSGNVSGLSQDVVAAAGA
YAHATPVIRRLAREFGVNLDKVKGSGRKGRIVKEDIQTYVKTAVQVFEKQGGTAAAATGAANGAGLGLLPWPKVDFSKFG
EVEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITDLEAFRKEQNKLAEKQKLDVKITPVVFIMKAVAKALEAFPRFN
SSISEDAQRLTLKKYINIGVAVDTPNGLVVPVFKNVNKKGIVELSRELMEVSKKARDGKLTASDMQGGCFTISSIGGLGT
THFTPIVNAPEVAILGVSKSEMAPVWNGKEFEPRLMLPLALSFDHRVIDGADGARFLTYINGVLADIRRLVM

Sequences:

>Translated_632_residues
MSKQINVPDIGSDEVSVTEVMVKAGDTITVDQSIINVEGDKASMEVPAPEAGVVKEVLVKVGDKVTTGSPMLVLEAAESA
PALVAEAPVQAVAPVATASAVVEVNVPDIGSDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPIAGVVKEILINV
GDKVTTGKLIMKFETVSAAPAAEEAPAQAAAPVATASAIKEVNVPDIGGDEVNVTEIMVNVGDTVSEEQSLITVEGDKAS
MEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAGAAPAAAPAPAVQAAAPAPAAQPAAQPAQSGNVSGLSQDVVAAAGA
YAHATPVIRRLAREFGVNLDKVKGSGRKGRIVKEDIQTYVKTAVQVFEKQGGTAAAATGAANGAGLGLLPWPKVDFSKFG
EVEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITDLEAFRKEQNKLAEKQKLDVKITPVVFIMKAVAKALEAFPRFN
SSISEDAQRLTLKKYINIGVAVDTPNGLVVPVFKNVNKKGIVELSRELMEVSKKARDGKLTASDMQGGCFTISSIGGLGT
THFTPIVNAPEVAILGVSKSEMAPVWNGKEFEPRLMLPLALSFDHRVIDGADGARFLTYINGVLADIRRLVM
>Mature_631_residues
SKQINVPDIGSDEVSVTEVMVKAGDTITVDQSIINVEGDKASMEVPAPEAGVVKEVLVKVGDKVTTGSPMLVLEAAESAP
ALVAEAPVQAVAPVATASAVVEVNVPDIGSDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPIAGVVKEILINVG
DKVTTGKLIMKFETVSAAPAAEEAPAQAAAPVATASAIKEVNVPDIGGDEVNVTEIMVNVGDTVSEEQSLITVEGDKASM
EVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAGAAPAAAPAPAVQAAAPAPAAQPAAQPAQSGNVSGLSQDVVAAAGAY
AHATPVIRRLAREFGVNLDKVKGSGRKGRIVKEDIQTYVKTAVQVFEKQGGTAAAATGAANGAGLGLLPWPKVDFSKFGE
VEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITDLEAFRKEQNKLAEKQKLDVKITPVVFIMKAVAKALEAFPRFNS
SISEDAQRLTLKKYINIGVAVDTPNGLVVPVFKNVNKKGIVELSRELMEVSKKARDGKLTASDMQGGCFTISSIGGLGTT
HFTPIVNAPEVAILGVSKSEMAPVWNGKEFEPRLMLPLALSFDHRVIDGADGARFLTYINGVLADIRRLVM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=444, Percent_Identity=30.8558558558559, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=30.0230946882217, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI19923748, Length=205, Percent_Identity=37.5609756097561, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI203098816, Length=238, Percent_Identity=33.1932773109244, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI203098753, Length=238, Percent_Identity=33.1932773109244, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI260898739, Length=143, Percent_Identity=37.0629370629371, Blast_Score=90, Evalue=6e-18,
Organism=Escherichia coli, GI1786305, Length=640, Percent_Identity=69.375, Blast_Score=812, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=411, Percent_Identity=28.4671532846715, Blast_Score=168, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=27.6190476190476, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=436, Percent_Identity=30.2752293577982, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI25146366, Length=207, Percent_Identity=38.6473429951691, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=28.7096774193548, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=423, Percent_Identity=29.5508274231678, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=441, Percent_Identity=27.891156462585, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI18859875, Length=418, Percent_Identity=30.3827751196172, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24645909, Length=218, Percent_Identity=35.7798165137615, Blast_Score=120, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=31.4893617021277, Blast_Score=119, Evalue=8e-27,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=31.4893617021277, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 66146; Mature: 66014

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQINVPDIGSDEVSVTEVMVKAGDTITVDQSIINVEGDKASMEVPAPEAGVVKEVLVK
CCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCEEEEECCCCCEEECCCCCHHHHHHHHHH
VGDKVTTGSPMLVLEAAESAPALVAEAPVQAVAPVATASAVVEVNVPDIGSDEVNVTEIM
CCCCCCCCCCEEEEEECCCCCCHHHHCCHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHE
VKVGDSVEVDQSIINVEGDKASMEVPAPIAGVVKEILINVGDKVTTGKLIMKFETVSAAP
EECCCCCCCCHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEECCEEEEEEEECCCCC
AAEEAPAQAAAPVATASAIKEVNVPDIGGDEVNVTEIMVNVGDTVSEEQSLITVEGDKAS
CCCCCCCHHHCCHHHHHHHHHCCCCCCCCCCEEHEEEEEECCCCCCCCCCEEEEECCCCC
MEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAGAAPAAAPAPAVQAAAPAPAAQPAAQ
EECCCCHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCCCCCCHHCCCCCCCCCCCCC
PAQSGNVSGLSQDVVAAAGAYAHATPVIRRLAREFGVNLDKVKGSGRKGRIVKEDIQTYV
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEHCCCCCCCCCEEHHHHHHHH
KTAVQVFEKQGGTAAAATGAANGAGLGLLPWPKVDFSKFGEVEEVELSRINKISGANLHR
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCC
NWVMIPHVTHFDRTDITDLEAFRKEQNKLAEKQKLDVKITPVVFIMKAVAKALEAFPRFN
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHCC
SSISEDAQRLTLKKYINIGVAVDTPNGLVVPVFKNVNKKGIVELSRELMEVSKKARDGKL
CHHHHHHHHHHHHHHHEEEEEEECCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHCCCCE
TASDMQGGCFTISSIGGLGTTHFTPIVNAPEVAILGVSKSEMAPVWNGKEFEPRLMLPLA
ECCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEE
LSFDHRVIDGADGARFLTYINGVLADIRRLVM
ECCCCEEECCCCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SKQINVPDIGSDEVSVTEVMVKAGDTITVDQSIINVEGDKASMEVPAPEAGVVKEVLVK
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCEEEEECCCCCEEECCCCCHHHHHHHHHH
VGDKVTTGSPMLVLEAAESAPALVAEAPVQAVAPVATASAVVEVNVPDIGSDEVNVTEIM
CCCCCCCCCCEEEEEECCCCCCHHHHCCHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHE
VKVGDSVEVDQSIINVEGDKASMEVPAPIAGVVKEILINVGDKVTTGKLIMKFETVSAAP
EECCCCCCCCHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEECCEEEEEEEECCCCC
AAEEAPAQAAAPVATASAIKEVNVPDIGGDEVNVTEIMVNVGDTVSEEQSLITVEGDKAS
CCCCCCCHHHCCHHHHHHHHHCCCCCCCCCCEEHEEEEEECCCCCCCCCCEEEEECCCCC
MEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAGAAPAAAPAPAVQAAAPAPAAQPAAQ
EECCCCHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCCCCCCHHCCCCCCCCCCCCC
PAQSGNVSGLSQDVVAAAGAYAHATPVIRRLAREFGVNLDKVKGSGRKGRIVKEDIQTYV
CCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEHCCCCCCCCCEEHHHHHHHH
KTAVQVFEKQGGTAAAATGAANGAGLGLLPWPKVDFSKFGEVEEVELSRINKISGANLHR
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCC
NWVMIPHVTHFDRTDITDLEAFRKEQNKLAEKQKLDVKITPVVFIMKAVAKALEAFPRFN
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHCC
SSISEDAQRLTLKKYINIGVAVDTPNGLVVPVFKNVNKKGIVELSRELMEVSKKARDGKL
CHHHHHHHHHHHHHHHEEEEEEECCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHCCCCE
TASDMQGGCFTISSIGGLGTTHFTPIVNAPEVAILGVSKSEMAPVWNGKEFEPRLMLPLA
ECCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEE
LSFDHRVIDGADGARFLTYINGVLADIRRLVM
ECCCCEEECCCCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]