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The map label for this gene is ushA [H]

Identifier: 126208247

GI number: 126208247

Start: 879630

End: 881273

Strand: Direct

Name: ushA [H]

Synonym: APL_0769

Alternate gene names: 126208247

Gene position: 879630-881273 (Clockwise)

Preceding gene: 126208246

Following gene: 126208248

Centisome position: 38.67

GC content: 40.21

Gene sequence:

>1644_bases
ATGAAATTTAATACAACGCTTCTCTGCACATCATTATTTTTTACAACTTCCGCAATCGCATATCAAGCGGATAAGACGTA
TTCTTTCACTCTGTTACATACTAATGATATTCACGGTCATTTTTGGCAGAATGAAAAAGGCGAATACGGATTAGCGGCAC
AAAAAACCTTAATCGACGGTATTAAGAAAGAAGTGGAAGCCAAAGGCGGTTCTACGATTATTTTAAATGCCGGCGATCTT
AATACCGGCGTACCGGAATCGGATATGCAAAACGCTCGTCCCGATTACGAAGGGCTGAACGCTATCGGCTATGAAGCCAT
GGTATTAGGTAATCATGAATTTGATTTCCCGCTTCAAGTGCTAAGTATGCAAGAAAAATGGGCGAAATTCCCGTTAATTT
CAGCTAACGTAATTAATAAAAAAACGCAAAAAGAATTGGTAAAACCTTATGTTATGCTTGATAAACAAGGATTAAAAGTC
GCCGTTGTCGGACTAACGACCGAGGATACCGGTAAATTAGGCAATCCCGACGTAACGGAAAACGTCGTCTTTAAAGATCC
GATTAAAACCGCTAAAGAAACATTAACTCAAATTAATCAAACCGAAAAGCCGGATATTCGTATCGCACTAACTCATATGG
GCTGGTATTTAGACGGTAAACACGGTACGAATGCACCCGGTGACGTAACAATGGCGAGAACGCTGGATAAAGGTGCGTTT
GATGTCATTATCGGCGGGCATACGCACGATACCGTGTGTTTTGACGATAAAGGCCAATTTAAAGCGAAATTCACACCGGG
TGAAAGCTGCAAACCGGATTTCCAAAACGGAACTTGGATTATGCAAGCCGGTGAATGGGGTAAATATGTCGGCCGCGCTG
ATTTCGAATTTAAAAACGGTAAAACGACACTGGTTAAATACGAGCTTATTCCGATTAATTTGAAAAAAACCATCAAAAAA
GAAGACGGTAAAAAAGAATATCAGCTTTACCAGCCGGAGATTAAAGCGGATCAAGCTCTCTTTGATCACTTGAAAAAATA
TCAAGACGAAGGCGATAAATTATTAGGTGTCGAAGTCGGTGAAGTAAAAGGTTTATTAGACGGAAAACGTGAACACGTAC
GTTTCTTCCAAACAAACTTAGGACGCTTAATCGCTCAATCGCAAAAAGAGCGTGTCAAAGCGGATATCGGCATTATGAAC
TCGGGCGGTGTACGCGCTTCGATTGAAGAAGGTAAAGTGACTTATAAAACGTTATTAACCGTTCAGCCTTTCGGTAATAT
GATTTCTACCGTAGATCTTAAAGGTCAAGAGTTACTGGATTTCTTAACCGCTATTGCATTAAAAGAAGTCGATACCGGTG
CTTATCCGCAATTTGCCGGTATTTCTATGAAAGTGGATCGTGCGGCAAAAGCAATCTCCGAGATTAAAATCGCAGGTAAA
GCCTTTGACCCGAATAAAACGTATAAAGTATCAATTCCTGACTACCTCACTGCCGGTGGCGACGGTTATCCCGTAATGAA
AAAGCATCCTAGCTACGTCAATACCGGTTTTATTGATGCCGAAATGTTGAAAAAATATTTTGAAGAAAATAAAGTCATCG
ACGCAAGCAAATTTGATCCTAAAGAAGACGTTATTTTTAAATAA

Upstream 100 bases:

>100_bases
TACTTATTCTTATTTTTTAAGGAGATATAGGTGATAGATAATCAGTGTAAAACGGATTATTTACCACCTATTTTTATATC
AATCAAAACTAGAGGTTATT

Downstream 100 bases:

>100_bases
AACCACAAGTCAAAAAAAGCTAAGCAATATGCTTAGCTTTTTTATTAGCTCATCACAATAAGAAGGGGTGTAATAGACAA
ATTTGTTGCTAAAAAGTGGC

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: UDP-sugar hydrolase; UDP-sugar diphosphatase; UDP-sugar pyrophosphatase; 5'-nucleotidase; 5'-NT [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MKFNTTLLCTSLFFTTSAIAYQADKTYSFTLLHTNDIHGHFWQNEKGEYGLAAQKTLIDGIKKEVEAKGGSTIILNAGDL
NTGVPESDMQNARPDYEGLNAIGYEAMVLGNHEFDFPLQVLSMQEKWAKFPLISANVINKKTQKELVKPYVMLDKQGLKV
AVVGLTTEDTGKLGNPDVTENVVFKDPIKTAKETLTQINQTEKPDIRIALTHMGWYLDGKHGTNAPGDVTMARTLDKGAF
DVIIGGHTHDTVCFDDKGQFKAKFTPGESCKPDFQNGTWIMQAGEWGKYVGRADFEFKNGKTTLVKYELIPINLKKTIKK
EDGKKEYQLYQPEIKADQALFDHLKKYQDEGDKLLGVEVGEVKGLLDGKREHVRFFQTNLGRLIAQSQKERVKADIGIMN
SGGVRASIEEGKVTYKTLLTVQPFGNMISTVDLKGQELLDFLTAIALKEVDTGAYPQFAGISMKVDRAAKAISEIKIAGK
AFDPNKTYKVSIPDYLTAGGDGYPVMKKHPSYVNTGFIDAEMLKKYFEENKVIDASKFDPKEDVIFK

Sequences:

>Translated_547_residues
MKFNTTLLCTSLFFTTSAIAYQADKTYSFTLLHTNDIHGHFWQNEKGEYGLAAQKTLIDGIKKEVEAKGGSTIILNAGDL
NTGVPESDMQNARPDYEGLNAIGYEAMVLGNHEFDFPLQVLSMQEKWAKFPLISANVINKKTQKELVKPYVMLDKQGLKV
AVVGLTTEDTGKLGNPDVTENVVFKDPIKTAKETLTQINQTEKPDIRIALTHMGWYLDGKHGTNAPGDVTMARTLDKGAF
DVIIGGHTHDTVCFDDKGQFKAKFTPGESCKPDFQNGTWIMQAGEWGKYVGRADFEFKNGKTTLVKYELIPINLKKTIKK
EDGKKEYQLYQPEIKADQALFDHLKKYQDEGDKLLGVEVGEVKGLLDGKREHVRFFQTNLGRLIAQSQKERVKADIGIMN
SGGVRASIEEGKVTYKTLLTVQPFGNMISTVDLKGQELLDFLTAIALKEVDTGAYPQFAGISMKVDRAAKAISEIKIAGK
AFDPNKTYKVSIPDYLTAGGDGYPVMKKHPSYVNTGFIDAEMLKKYFEENKVIDASKFDPKEDVIFK
>Mature_547_residues
MKFNTTLLCTSLFFTTSAIAYQADKTYSFTLLHTNDIHGHFWQNEKGEYGLAAQKTLIDGIKKEVEAKGGSTIILNAGDL
NTGVPESDMQNARPDYEGLNAIGYEAMVLGNHEFDFPLQVLSMQEKWAKFPLISANVINKKTQKELVKPYVMLDKQGLKV
AVVGLTTEDTGKLGNPDVTENVVFKDPIKTAKETLTQINQTEKPDIRIALTHMGWYLDGKHGTNAPGDVTMARTLDKGAF
DVIIGGHTHDTVCFDDKGQFKAKFTPGESCKPDFQNGTWIMQAGEWGKYVGRADFEFKNGKTTLVKYELIPINLKKTIKK
EDGKKEYQLYQPEIKADQALFDHLKKYQDEGDKLLGVEVGEVKGLLDGKREHVRFFQTNLGRLIAQSQKERVKADIGIMN
SGGVRASIEEGKVTYKTLLTVQPFGNMISTVDLKGQELLDFLTAIALKEVDTGAYPQFAGISMKVDRAAKAISEIKIAGK
AFDPNKTYKVSIPDYLTAGGDGYPVMKKHPSYVNTGFIDAEMLKKYFEENKVIDASKFDPKEDVIFK

Specific function: Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm. Note=Exported from the cell, except a small proportion that is internally localized [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=564, Percent_Identity=25.709219858156, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1786687, Length=542, Percent_Identity=53.1365313653137, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1790658, Length=574, Percent_Identity=22.1254355400697, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28573524, Length=561, Percent_Identity=27.0944741532977, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI19922444, Length=556, Percent_Identity=25.7194244604317, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24641187, Length=552, Percent_Identity=24.8188405797101, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922446, Length=527, Percent_Identity=26.7552182163188, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24654424, Length=527, Percent_Identity=26.7552182163188, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI221329836, Length=551, Percent_Identity=21.5970961887477, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.6.1.45; =3.1.3.5 [H]

Molecular weight: Translated: 60896; Mature: 60896

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNTTLLCTSLFFTTSAIAYQADKTYSFTLLHTNDIHGHFWQNEKGEYGLAAQKTLIDG
CCCCHHHHHHHHHHHHHHHEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
IKKEVEAKGGSTIILNAGDLNTGVPESDMQNARPDYEGLNAIGYEAMVLGNHEFDFPLQV
HHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHH
LSMQEKWAKFPLISANVINKKTQKELVKPYVMLDKQGLKVAVVGLTTEDTGKLGNPDVTE
HHHHHHHHCCCEEEHHHHCHHHHHHHHCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCC
NVVFKDPIKTAKETLTQINQTEKPDIRIALTHMGWYLDGKHGTNAPGDVTMARTLDKGAF
CCEECCHHHHHHHHHHHCCCCCCCCEEEEEEEECEEEECCCCCCCCCCEEEEEECCCCCE
DVIIGGHTHDTVCFDDKGQFKAKFTPGESCKPDFQNGTWIMQAGEWGKYVGRADFEFKNG
EEEECCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEECCC
KTTLVKYELIPINLKKTIKKEDGKKEYQLYQPEIKADQALFDHLKKYQDEGDKLLGVEVG
CEEEEEEEEEEECHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEHH
EVKGLLDGKREHVRFFQTNLGRLIAQSQKERVKADIGIMNSGGVRASIEEGKVTYKTLLT
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCEEEEEEEE
VQPFGNMISTVDLKGQELLDFLTAIALKEVDTGAYPQFAGISMKVDRAAKAISEIKIAGK
ECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHEEECC
AFDPNKTYKVSIPDYLTAGGDGYPVMKKHPSYVNTGFIDAEMLKKYFEENKVIDASKFDP
EECCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCC
KEDVIFK
CCCCCCC
>Mature Secondary Structure
MKFNTTLLCTSLFFTTSAIAYQADKTYSFTLLHTNDIHGHFWQNEKGEYGLAAQKTLIDG
CCCCHHHHHHHHHHHHHHHEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
IKKEVEAKGGSTIILNAGDLNTGVPESDMQNARPDYEGLNAIGYEAMVLGNHEFDFPLQV
HHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHH
LSMQEKWAKFPLISANVINKKTQKELVKPYVMLDKQGLKVAVVGLTTEDTGKLGNPDVTE
HHHHHHHHCCCEEEHHHHCHHHHHHHHCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCC
NVVFKDPIKTAKETLTQINQTEKPDIRIALTHMGWYLDGKHGTNAPGDVTMARTLDKGAF
CCEECCHHHHHHHHHHHCCCCCCCCEEEEEEEECEEEECCCCCCCCCCEEEEEECCCCCE
DVIIGGHTHDTVCFDDKGQFKAKFTPGESCKPDFQNGTWIMQAGEWGKYVGRADFEFKNG
EEEECCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEECCC
KTTLVKYELIPINLKKTIKKEDGKKEYQLYQPEIKADQALFDHLKKYQDEGDKLLGVEVG
CEEEEEEEEEEECHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEHH
EVKGLLDGKREHVRFFQTNLGRLIAQSQKERVKADIGIMNSGGVRASIEEGKVTYKTLLT
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCEEEEEEEE
VQPFGNMISTVDLKGQELLDFLTAIALKEVDTGAYPQFAGISMKVDRAAKAISEIKIAGK
ECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHEEECC
AFDPNKTYKVSIPDYLTAGGDGYPVMKKHPSYVNTGFIDAEMLKKYFEENKVIDASKFDP
EECCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCC
KEDVIFK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3012467; 9278503; 9298646; 10331872; 11491293; 11491294 [H]