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The map label for this gene is fabI [H]

Identifier: 126208233

GI number: 126208233

Start: 865141

End: 865929

Strand: Direct

Name: fabI [H]

Synonym: APL_0755

Alternate gene names: 126208233

Gene position: 865141-865929 (Clockwise)

Preceding gene: 126208232

Following gene: 126208234

Centisome position: 38.04

GC content: 44.74

Gene sequence:

>789_bases
ATGAGCTTTTTAACAGGTAAACGCATTTTAGTAACGGGTCTTGCAAGCAACCGTTCTATCGCATACGGCATTGCAAAAGC
AATGAAAGCACAAGGCGCAGAACTCGCATTCACTTATTTAAACGATAAATTACAACCGCGTGTTGAAGAATTTGCTAAAG
AATTCGGTTCGGACATCGTATTACCATTAGATGTAGCGACAGACGAATCAATCCAAAACTGCTTTGCAGAATTAAGCAAA
AAATGGGAAAAATTCGACGGTTTCGTACACGCCATCGCATTCGCACCGGGCGATCAATTAGACGGTGATTATGTAAACGC
GGCAACACGCGAAGGCTACCGTATCGCACACGACATCAGCGCATACAGCTTCGTTGCAATGGCTCAAGCGGCTCGTCCGT
ACTTAAACGAAAATGCGGCACTTTTAACCTTAAGCTACTTAGGTGCGGAACGTGCGATTCCTAACTACAACGTAATGTGT
TTAGCAAAAGCGTCATTAGAAGCGGCGACGCGTGTAATGGCGGCGGATTTAGGTAAAGACGGTATTCGTGTAAATGCAAT
CTCTGCAGGTCCAATCCGTACACTTGCGGCATCAGGCATCAAAAACTTCAAAAAAATGCTTTCTGCATTCGAGAAAACCG
CAGCATTACGTCGTACCGTTACCATTGACGATGTAGGTAACTCGGCGGCATTCTTATGTTCGGACTTAGCTTCAGGTGTA
ACCGGTGAAGTATTACACGTTGATGCAGGTTTCAGCATTACTGCAATGGGTGAATTAGGCGAAGAATAA

Upstream 100 bases:

>100_bases
GTATAATCGATAAAATCTCGTAGGGGCGTATCGAATACGTCCTTTATTTTATATGAAGTAGGCGTGTTACACGCTTCAAC
AATATTCAAAGGAATCCAGC

Downstream 100 bases:

>100_bases
TTCGGCTTGAATATATAAGTATGTAAAACGGACGCAATTTAGCGTCCGTTTTTTATTTCTGCGATCTTGATCGAAGAATC
AGAATTTTCGCCAGTCCTAA

Product: enoyl-[acyl-carrier-protein] reductase (NADH)

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSFLTGKRILVTGLASNRSIAYGIAKAMKAQGAELAFTYLNDKLQPRVEEFAKEFGSDIVLPLDVATDESIQNCFAELSK
KWEKFDGFVHAIAFAPGDQLDGDYVNAATREGYRIAHDISAYSFVAMAQAARPYLNENAALLTLSYLGAERAIPNYNVMC
LAKASLEAATRVMAADLGKDGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRRTVTIDDVGNSAAFLCSDLASGV
TGEVLHVDAGFSITAMGELGEE

Sequences:

>Translated_262_residues
MSFLTGKRILVTGLASNRSIAYGIAKAMKAQGAELAFTYLNDKLQPRVEEFAKEFGSDIVLPLDVATDESIQNCFAELSK
KWEKFDGFVHAIAFAPGDQLDGDYVNAATREGYRIAHDISAYSFVAMAQAARPYLNENAALLTLSYLGAERAIPNYNVMC
LAKASLEAATRVMAADLGKDGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRRTVTIDDVGNSAAFLCSDLASGV
TGEVLHVDAGFSITAMGELGEE
>Mature_261_residues
SFLTGKRILVTGLASNRSIAYGIAKAMKAQGAELAFTYLNDKLQPRVEEFAKEFGSDIVLPLDVATDESIQNCFAELSKK
WEKFDGFVHAIAFAPGDQLDGDYVNAATREGYRIAHDISAYSFVAMAQAARPYLNENAALLTLSYLGAERAIPNYNVMCL
AKASLEAATRVMAADLGKDGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRRTVTIDDVGNSAAFLCSDLASGVT
GEVLHVDAGFSITAMGELGEE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503301, Length=240, Percent_Identity=27.5, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI7705925, Length=258, Percent_Identity=25.5813953488372, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=74.1312741312741, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI87082100, Length=263, Percent_Identity=26.615969581749, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789378, Length=251, Percent_Identity=26.6932270916335, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=259, Percent_Identity=28.1853281853282, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=275, Percent_Identity=28, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644337, Length=120, Percent_Identity=36.6666666666667, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28007; Mature: 27876

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLTGKRILVTGLASNRSIAYGIAKAMKAQGAELAFTYLNDKLQPRVEEFAKEFGSDIV
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCEE
LPLDVATDESIQNCFAELSKKWEKFDGFVHAIAFAPGDQLDGDYVNAATREGYRIAHDIS
EEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHCCCEEEEHHHH
AYSFVAMAQAARPYLNENAALLTLSYLGAERAIPNYNVMCLAKASLEAATRVMAADLGKD
HHHHHHHHHHHCHHHCCCCEEEEEEHHCHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCC
GIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRRTVTIDDVGNSAAFLCSDLASGV
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCC
TGEVLHVDAGFSITAMGELGEE
CCCEEEEECCCEEEECCCCCCC
>Mature Secondary Structure 
SFLTGKRILVTGLASNRSIAYGIAKAMKAQGAELAFTYLNDKLQPRVEEFAKEFGSDIV
CCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCEE
LPLDVATDESIQNCFAELSKKWEKFDGFVHAIAFAPGDQLDGDYVNAATREGYRIAHDIS
EEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHCCCEEEEHHHH
AYSFVAMAQAARPYLNENAALLTLSYLGAERAIPNYNVMCLAKASLEAATRVMAADLGKD
HHHHHHHHHHHCHHHCCCCEEEEEEHHCHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCC
GIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRRTVTIDDVGNSAAFLCSDLASGV
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCC
TGEVLHVDAGFSITAMGELGEE
CCCEEEEECCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]