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The map label for this gene is yecO

Identifier: 126208232

GI number: 126208232

Start: 864217

End: 864942

Strand: Direct

Name: yecO

Synonym: APL_0754

Alternate gene names: 126208232

Gene position: 864217-864942 (Clockwise)

Preceding gene: 126208231

Following gene: 126208233

Centisome position: 38.0

GC content: 41.46

Gene sequence:

>726_bases
ATGAATAAAGATACGATTTTTTCCGCACCGATTGAGAAACTCGGTGACTTTACCTTTGACGAATCGGTTGCCGAAGTGTT
TCCCGATATGATCCAACGTTCCGTACCGGGCTACTCGAATATCATTACCGCAATCGGTATGTTGGCACAACGTTTCGTTA
CCGAAGGATCGCAAGTTTATGATTTAGGTTGTTCTCGTGGTGCCGGCATTTTATCAATACGCCGCAACCTACAAACCAAT
CAAGTAAAAATTATCGGTGTAGATAATTCTCAACCGATGGTGGAACGCTGCCGCTCGCATATTAATGCTTATCACTCTGA
AGTACCGGTAGAGATCCTTTGTGACGATATTCGCCATATCGAAATCAAAAACGCTTCAATGGTCGTGTTGAATTTTACCT
TGCAATTTTTGCCTCGTGCGGATCGTCTTGAATTGCTGACTAAAATTTATCAGGGCTTAAACCCGAACGGTATTTTGGTG
CTATCTGAAAAGTTCACTTTTGAAAACCAAGTCATGAATGAATTACTCATTGACTTACATCATACCTTTAAACGTGCTAA
CGGTTACAGCGAACTTGAAGTCAGCCAAAAACGTACCGCTCTCGAAAACGTGATGCTGACCGATAGCATTGAAACCCATA
AAGATCGCTTAAAACAAGCCGGTTTTTCCCAAATCGAACTGTGGTTCCAATGTTTTAATTTCGGCTCGATGATTGCAGTT
AAATAA

Upstream 100 bases:

>100_bases
TCGTTTGTCGTCGCCAATGCGCAACACTGGGTACACGCGGAAAAACCGGAAACGGTCGCTCGCGCCATTCAAAAATTTTT
AGACAAGGAATAATAACATT

Downstream 100 bases:

>100_bases
GCGGTCAAATTTGCGAAAAACTTTGCAAAAACTGCTCGGATCAGACTGCTTGCTCAACCTGAAACCTTAGGAAATTTGCG
CAAGCGGTCATTTTTTCGGT

Product: S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MNKDTIFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITAIGMLAQRFVTEGSQVYDLGCSRGAGILSIRRNLQTN
QVKIIGVDNSQPMVERCRSHINAYHSEVPVEILCDDIRHIEIKNASMVVLNFTLQFLPRADRLELLTKIYQGLNPNGILV
LSEKFTFENQVMNELLIDLHHTFKRANGYSELEVSQKRTALENVMLTDSIETHKDRLKQAGFSQIELWFQCFNFGSMIAV
K

Sequences:

>Translated_241_residues
MNKDTIFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITAIGMLAQRFVTEGSQVYDLGCSRGAGILSIRRNLQTN
QVKIIGVDNSQPMVERCRSHINAYHSEVPVEILCDDIRHIEIKNASMVVLNFTLQFLPRADRLELLTKIYQGLNPNGILV
LSEKFTFENQVMNELLIDLHHTFKRANGYSELEVSQKRTALENVMLTDSIETHKDRLKQAGFSQIELWFQCFNFGSMIAV
K
>Mature_241_residues
MNKDTIFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITAIGMLAQRFVTEGSQVYDLGCSRGAGILSIRRNLQTN
QVKIIGVDNSQPMVERCRSHINAYHSEVPVEILCDDIRHIEIKNASMVVLNFTLQFLPRADRLELLTKIYQGLNPNGILV
LSEKFTFENQVMNELLIDLHHTFKRANGYSELEVSQKRTALENVMLTDSIETHKDRLKQAGFSQIELWFQCFNFGSMIAV
K

Specific function: Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U)

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. CmoA family

Homologues:

Organism=Escherichia coli, GI1788177, Length=240, Percent_Identity=69.1666666666667, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CMOA_ACTP2 (A3N0B6)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053457.1
- ProteinModelPortal:   A3N0B6
- SMR:   A3N0B6
- STRING:   A3N0B6
- GeneID:   4850360
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0754
- eggNOG:   COG0500
- HOGENOM:   HBG296860
- OMA:   YSELEVS
- PhylomeDB:   A3N0B6
- ProtClustDB:   CLSK870321
- BioCyc:   APLE416269:APL_0754-MONOMER
- HAMAP:   MF_01589
- InterPro:   IPR013216
- InterPro:   IPR005271
- PIRSF:   PIRSF006325
- TIGRFAMs:   TIGR00740

Pfam domain/function: PF08241 Methyltransf_11

EC number: NA

Molecular weight: Translated: 27392; Mature: 27392

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: BINDING 38-38 BINDING 88-88 BINDING 131-131 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDTIFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITAIGMLAQRFVTEGSQVY
CCCCCEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEE
DLGCSRGAGILSIRRNLQTNQVKIIGVDNSQPMVERCRSHINAYHSEVPVEILCDDIRHI
ECCCCCCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHEE
EIKNASMVVLNFTLQFLPRADRLELLTKIYQGLNPNGILVLSEKFTFENQVMNELLIDLH
EECCCCEEEEEHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHH
HTFKRANGYSELEVSQKRTALENVMLTDSIETHKDRLKQAGFSQIELWFQCFNFGSMIAV
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEE
K
C
>Mature Secondary Structure
MNKDTIFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITAIGMLAQRFVTEGSQVY
CCCCCEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEE
DLGCSRGAGILSIRRNLQTNQVKIIGVDNSQPMVERCRSHINAYHSEVPVEILCDDIRHI
ECCCCCCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHEE
EIKNASMVVLNFTLQFLPRADRLELLTKIYQGLNPNGILVLSEKFTFENQVMNELLIDLH
EECCCCEEEEEHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHH
HTFKRANGYSELEVSQKRTALENVMLTDSIETHKDRLKQAGFSQIELWFQCFNFGSMIAV
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEE
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA