Click here to switch to the map view.

The map label for this gene is topB2 [H]

Identifier: 126208224

GI number: 126208224

Start: 849112

End: 851049

Strand: Reverse

Name: topB2 [H]

Synonym: APL_0744

Alternate gene names: 126208224

Gene position: 851049-849112 (Counterclockwise)

Preceding gene: 126208225

Following gene: 126208223

Centisome position: 37.42

GC content: 43.24

Gene sequence:

>1938_bases
ATGATATCAGAGAAAAGAATTTTGAGAGTATTTTTAGCGGAAAAACCGAGTTTGGCAAGAGCAATCGCAGACGTATTGCC
GAAGCCGCATACCCGTGGTGACGGCTATATTCAATGTGGTGAAAATGATACGGTAACTTGGTGTATCGGTCATTTATTGG
AACAAGCGGAACCTGATATTTACGACGAACGTTTTAAAATGTGGCGAATGGAGCATTTGCCGATTGTCCCGCAGCAATGG
AAATTAGTGCCGAAAAAAGAAACGTTGAAGCAGTTAAAAGCGGTCGAAAAATTGGTAAAAAAAGCGGACGTATTGGTAAA
CGCCGGCGACCCGGACCGAGAAGGGCAGCTATTAGTTGATGAGGTCTTTAGCTATCTGAACGTACCGCCGGAAAAACGTA
ATCGGATTCAACGTTGTTTGGTGAGCGATCTCAACCCGAGTGCGGTAAAAAAAGCGATTGATAAATTGCAAAATAATCGT
GATTTTATACCGCTTGCCACCTCTGCCTTAGCACGTGCAAGAGCCGATTGGCTATACGGCATTAATATGACGCGAGCTTA
TACCTTACAAGGACGTTGGGCGGGCTATCAAGGGGTATTGTCGGTGGGGCGTGTTCAAACCCCCGTGTTAGGTTTAATTG
TGCGCCGCGATCTGGAAATAGAAAACTTTGTGCCGAAAGACTATTTTGAAGTATTGGCGCATATCGTTGTGCCGGAAACT
CAAGAACGTTTTACTGCCCAATGGCAACCGAGTAAGGCGTGCGAAGATTATCAAGATGAAGACGGTCGAGTATTATCTAA
AAGTTTGGCGGAAAATGTCGTTAAACGCATTACCGACCAAGCAGCATTAGTAACGGATTATCAAGATAAAATTGAAACGG
AAACCGCACCGCTGCCTTATTCATTATCGGTATTACAAATTGATGCGGCACGCCGTTACGGCCTATCGGCACAAGCTGTG
TTGGATATTTGTCAGAAACTGTATGAAACGCATAAGCTGATTACCTATCCCCGTTCGGATAACCGTTATCTACCGAATGA
GCATTACGCTGATCGTTTTAAAGTGATGGCGGCGATTGCACATCATCTGGATGAGTATAAAGAAAAACCGGAGGCGGTTA
ATCCGGAAATTCGTAATCGTTGTTGGAATGATAAAAAGGTAGAAGCGCACCATGCTATTATTCCGACCGTACGCCAAGGC
TCGGTAAAACTAACTGAAAACGAAAAGCGTATTTATCAACTGATTGCCCGTCAATACTTAATGCAATTTTGTCCGGATGC
GGAATATCGCAAAGGTAAAATTGTATTGGAGATTGCCGGAGGAACTTTTAACGCGCAGGCTCGGAACTTAATTGTTGCCG
GTTGGAAAGCATTATTAGGCAAAGAAGACAGTGACGAAGTGTTAGAGCCGTTATTACCGGTTGTGAAAAAAGGGCAGCAA
TTACATTGTGAAAAAGGTGAGATTGTCAGTAAGAAAACCCAGCCGCCTCGTCATTTTACCGACGCAAGTTTATTATCGGC
AATGACCGGTATCGCTCGTTTCGTACAAGATAAACAACTTAAAAAAGTCTTACGTGAAACGGACGGCTTAGGTACTGAAG
CAACACGTGCCGGTATTATCGAATTGCTGTTTAAACGCGGATTTTTAATCAAAAAAGGACGAAATATCCACAGTACGGAA
GCCGGGCGAATTCTAATTAGTGCCTTACCGGACGAAGCGACTCAGCCGGATATGACTGCGCATTGGGAAATGCAACTTAC
CGACATTAGTAAGAAGCAAGCGAGTTATCAGCAGTTTATGCAAGTACTTACGAGTAAATTACCTGAGTTATTACATACGG
AACGACAAAAATTGCAAAATTTAAGTCGAATTCAACCGCCCGCCTCTTATCGTAAGCGGTCAAATTTTAAGAAAAATTTA
CAAACTGCTGAAAAATAA

Upstream 100 bases:

>100_bases
TTTCTATGACAGTAAATATTTTCCGTAAGAAAGGCGGATATAAGACTCGCTATTGCTTTTTAAAAAGAAAAATCAGACAA
TTTTCATTAATAAAATCAGA

Downstream 100 bases:

>100_bases
GGCAAATGTATCGTTCGGATAAAAAAATTACTTGCAAGCGAATGGCTAAATCTATATGATGCACGCCATCGCAATTTGAT
TTTAACTTTAAAATCTGGAA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MISEKRILRVFLAEKPSLARAIADVLPKPHTRGDGYIQCGENDTVTWCIGHLLEQAEPDIYDERFKMWRMEHLPIVPQQW
KLVPKKETLKQLKAVEKLVKKADVLVNAGDPDREGQLLVDEVFSYLNVPPEKRNRIQRCLVSDLNPSAVKKAIDKLQNNR
DFIPLATSALARARADWLYGINMTRAYTLQGRWAGYQGVLSVGRVQTPVLGLIVRRDLEIENFVPKDYFEVLAHIVVPET
QERFTAQWQPSKACEDYQDEDGRVLSKSLAENVVKRITDQAALVTDYQDKIETETAPLPYSLSVLQIDAARRYGLSAQAV
LDICQKLYETHKLITYPRSDNRYLPNEHYADRFKVMAAIAHHLDEYKEKPEAVNPEIRNRCWNDKKVEAHHAIIPTVRQG
SVKLTENEKRIYQLIARQYLMQFCPDAEYRKGKIVLEIAGGTFNAQARNLIVAGWKALLGKEDSDEVLEPLLPVVKKGQQ
LHCEKGEIVSKKTQPPRHFTDASLLSAMTGIARFVQDKQLKKVLRETDGLGTEATRAGIIELLFKRGFLIKKGRNIHSTE
AGRILISALPDEATQPDMTAHWEMQLTDISKKQASYQQFMQVLTSKLPELLHTERQKLQNLSRIQPPASYRKRSNFKKNL
QTAEK

Sequences:

>Translated_645_residues
MISEKRILRVFLAEKPSLARAIADVLPKPHTRGDGYIQCGENDTVTWCIGHLLEQAEPDIYDERFKMWRMEHLPIVPQQW
KLVPKKETLKQLKAVEKLVKKADVLVNAGDPDREGQLLVDEVFSYLNVPPEKRNRIQRCLVSDLNPSAVKKAIDKLQNNR
DFIPLATSALARARADWLYGINMTRAYTLQGRWAGYQGVLSVGRVQTPVLGLIVRRDLEIENFVPKDYFEVLAHIVVPET
QERFTAQWQPSKACEDYQDEDGRVLSKSLAENVVKRITDQAALVTDYQDKIETETAPLPYSLSVLQIDAARRYGLSAQAV
LDICQKLYETHKLITYPRSDNRYLPNEHYADRFKVMAAIAHHLDEYKEKPEAVNPEIRNRCWNDKKVEAHHAIIPTVRQG
SVKLTENEKRIYQLIARQYLMQFCPDAEYRKGKIVLEIAGGTFNAQARNLIVAGWKALLGKEDSDEVLEPLLPVVKKGQQ
LHCEKGEIVSKKTQPPRHFTDASLLSAMTGIARFVQDKQLKKVLRETDGLGTEATRAGIIELLFKRGFLIKKGRNIHSTE
AGRILISALPDEATQPDMTAHWEMQLTDISKKQASYQQFMQVLTSKLPELLHTERQKLQNLSRIQPPASYRKRSNFKKNL
QTAEK
>Mature_645_residues
MISEKRILRVFLAEKPSLARAIADVLPKPHTRGDGYIQCGENDTVTWCIGHLLEQAEPDIYDERFKMWRMEHLPIVPQQW
KLVPKKETLKQLKAVEKLVKKADVLVNAGDPDREGQLLVDEVFSYLNVPPEKRNRIQRCLVSDLNPSAVKKAIDKLQNNR
DFIPLATSALARARADWLYGINMTRAYTLQGRWAGYQGVLSVGRVQTPVLGLIVRRDLEIENFVPKDYFEVLAHIVVPET
QERFTAQWQPSKACEDYQDEDGRVLSKSLAENVVKRITDQAALVTDYQDKIETETAPLPYSLSVLQIDAARRYGLSAQAV
LDICQKLYETHKLITYPRSDNRYLPNEHYADRFKVMAAIAHHLDEYKEKPEAVNPEIRNRCWNDKKVEAHHAIIPTVRQG
SVKLTENEKRIYQLIARQYLMQFCPDAEYRKGKIVLEIAGGTFNAQARNLIVAGWKALLGKEDSDEVLEPLLPVVKKGQQ
LHCEKGEIVSKKTQPPRHFTDASLLSAMTGIARFVQDKQLKKVLRETDGLGTEATRAGIIELLFKRGFLIKKGRNIHSTE
AGRILISALPDEATQPDMTAHWEMQLTDISKKQASYQQFMQVLTSKLPELLHTERQKLQNLSRIQPPASYRKRSNFKKNL
QTAEK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=622, Percent_Identity=22.1864951768489, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI10835218, Length=604, Percent_Identity=24.1721854304636, Blast_Score=91, Evalue=4e-18,
Organism=Escherichia coli, GI1788061, Length=624, Percent_Identity=66.3461538461538, Blast_Score=862, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=548, Percent_Identity=26.4598540145985, Blast_Score=123, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32563869, Length=616, Percent_Identity=22.8896103896104, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17555378, Length=651, Percent_Identity=22.273425499232, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640096, Length=633, Percent_Identity=23.3807266982622, Blast_Score=109, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24585251, Length=590, Percent_Identity=23.3898305084746, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 73725; Mature: 73725

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISEKRILRVFLAEKPSLARAIADVLPKPHTRGDGYIQCGENDTVTWCIGHLLEQAEPDI
CCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCH
YDERFKMWRMEHLPIVPQQWKLVPKKETLKQLKAVEKLVKKADVLVNAGDPDREGQLLVD
HHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHH
EVFSYLNVPPEKRNRIQRCLVSDLNPSAVKKAIDKLQNNRDFIPLATSALARARADWLYG
HHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
INMTRAYTLQGRWAGYQGVLSVGRVQTPVLGLIVRRDLEIENFVPKDYFEVLAHIVVPET
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHCCCH
QERFTAQWQPSKACEDYQDEDGRVLSKSLAENVVKRITDQAALVTDYQDKIETETAPLPY
HHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCC
SLSVLQIDAARRYGLSAQAVLDICQKLYETHKLITYPRSDNRYLPNEHYADRFKVMAAIA
EEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
HHLDEYKEKPEAVNPEIRNRCWNDKKVEAHHAIIPTVRQGSVKLTENEKRIYQLIARQYL
HHHHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHH
MQFCPDAEYRKGKIVLEIAGGTFNAQARNLIVAGWKALLGKEDSDEVLEPLLPVVKKGQQ
HHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCE
LHCEKGEIVSKKTQPPRHFTDASLLSAMTGIARFVQDKQLKKVLRETDGLGTEATRAGII
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ELLFKRGFLIKKGRNIHSTEAGRILISALPDEATQPDMTAHWEMQLTDISKKQASYQQFM
HHHHHCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHH
QVLTSKLPELLHTERQKLQNLSRIQPPASYRKRSNFKKNLQTAEK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISEKRILRVFLAEKPSLARAIADVLPKPHTRGDGYIQCGENDTVTWCIGHLLEQAEPDI
CCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCH
YDERFKMWRMEHLPIVPQQWKLVPKKETLKQLKAVEKLVKKADVLVNAGDPDREGQLLVD
HHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHH
EVFSYLNVPPEKRNRIQRCLVSDLNPSAVKKAIDKLQNNRDFIPLATSALARARADWLYG
HHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
INMTRAYTLQGRWAGYQGVLSVGRVQTPVLGLIVRRDLEIENFVPKDYFEVLAHIVVPET
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHCCCH
QERFTAQWQPSKACEDYQDEDGRVLSKSLAENVVKRITDQAALVTDYQDKIETETAPLPY
HHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCC
SLSVLQIDAARRYGLSAQAVLDICQKLYETHKLITYPRSDNRYLPNEHYADRFKVMAAIA
EEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
HHLDEYKEKPEAVNPEIRNRCWNDKKVEAHHAIIPTVRQGSVKLTENEKRIYQLIARQYL
HHHHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHH
MQFCPDAEYRKGKIVLEIAGGTFNAQARNLIVAGWKALLGKEDSDEVLEPLLPVVKKGQQ
HHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCE
LHCEKGEIVSKKTQPPRHFTDASLLSAMTGIARFVQDKQLKKVLRETDGLGTEATRAGII
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ELLFKRGFLIKKGRNIHSTEAGRILISALPDEATQPDMTAHWEMQLTDISKKQASYQQFM
HHHHHCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHH
QVLTSKLPELLHTERQKLQNLSRIQPPASYRKRSNFKKNLQTAEK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]