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The map label for this gene is ackA

Identifier: 126208127

GI number: 126208127

Start: 738008

End: 739216

Strand: Reverse

Name: ackA

Synonym: APL_0645

Alternate gene names: 126208127

Gene position: 739216-738008 (Counterclockwise)

Preceding gene: 126208129

Following gene: 126208126

Centisome position: 32.5

GC content: 42.6

Gene sequence:

>1209_bases
ATGTCTAAAAACTTAATCCTTATCCTTAACTGCGGTAGTTCTTCTTTAAAATTTGCGGTGTTAGATCCTAAAACGGGTGA
CGAGAAATTATCCGGTTTAGCGGAAGCATTTAATTTAGAAGATGCTCGTATTAAATGGAAATTACACGGCGAAAAAGGCA
ATGCAGATTTAGGTGCGGGTGCAGCGCATAGCGAAGCATTAACTTTCATTGCAAATGAATTATTATCAGAAGAATTAAGA
TCCAGTATCGGTGCTATCGGTCACCGTATCGTTCACGGTGGTGAGCAATTCACTTCATCCGTAGTAATCAATGATGATGT
AGTTAAAGGCATCGAACACGCAATTCAATTCGCACCGCTTCACAATCCGGCGCATTTAATCGGTATCAAAGAAGCATTCC
GTATTTTCCCTGAGTTAAAAGAGAAAAACGTTGCGGTATTCGATACGGCTTTCCACCAAACTATGCCGGAAGAAGCATTT
TTATACGCACTTCCGTACAAACTCTATAAAGAACACGGTATCCGTCGTTACGGCGCTCACGGTACCAGCCATTTATTCAT
CACCTCACAAGTTGCCGAATTAGCGGGCAAACCGGTAGATCAAACCAATGCGATTATCTGTCACTTAGGTAACGGCGGTT
CCGTATCTGTGGTACGTAACGGTAAATGTATCGATACGTCAATGGGTTTAACACCGTTAGAAGGTTTAGTGATGGGGACT
CGTTCGGGCGATATCGACCCTGCAATCGTTTTCTACCTATACAAAAACTTAGGTATGTCAATGGAGCAAATCGAAGATAC
GTTAGTGAAAAAATCGGGTCTTTTAGGTTTAACTGAAGTAACCAGCGACTGTCGTTATGCGGAAGATAACTACGAGGACG
CGTCAAAACCTGAAGCAAAACGTGCATTAGACGTATATAGCTACCGTTTAGCAAAATACATCGGTGCATATATGGCGATC
TTAGGTGATGACCACTTAGACGCTATCGCATTTACCGGCGGTATCGGTGAGAACTCGGGTCACGTACGTGAATTAGCGTT
AAATCACTTAAAATTATTCGGCGTTAAATTAGACGTTGAACGTAACTTAGCAGCGCGCTTCGGTAAATCCGGCGTTATCA
CCGCGGACGACTCAACATTCAAAGCGGTCGTAATTCCGACTAACGAAGAATTAGTGATTGCACAAGATACGGCTAAATTA
GCACTATAA

Upstream 100 bases:

>100_bases
ATTTTAGCGCATGAAACGTGTATGCTTATAAAGACGTACGCATATTTGCGTCACACAAGTTATCAATCCTTTCATAAACA
AAGAAATAAAGGTGTTTGAC

Downstream 100 bases:

>100_bases
TTTCCAAGGGCAGATATTCTGCCCTTTTACTATTTCACACAAACGGAATTTAAATATGTCCAGAACTATTATCTTAATCC
CAACAACAACCGGTGTCGGC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSKNLILILNCGSSSLKFAVLDPKTGDEKLSGLAEAFNLEDARIKWKLHGEKGNADLGAGAAHSEALTFIANELLSEELR
SSIGAIGHRIVHGGEQFTSSVVINDDVVKGIEHAIQFAPLHNPAHLIGIKEAFRIFPELKEKNVAVFDTAFHQTMPEEAF
LYALPYKLYKEHGIRRYGAHGTSHLFITSQVAELAGKPVDQTNAIICHLGNGGSVSVVRNGKCIDTSMGLTPLEGLVMGT
RSGDIDPAIVFYLYKNLGMSMEQIEDTLVKKSGLLGLTEVTSDCRYAEDNYEDASKPEAKRALDVYSYRLAKYIGAYMAI
LGDDHLDAIAFTGGIGENSGHVRELALNHLKLFGVKLDVERNLAARFGKSGVITADDSTFKAVVIPTNEELVIAQDTAKL
AL

Sequences:

>Translated_402_residues
MSKNLILILNCGSSSLKFAVLDPKTGDEKLSGLAEAFNLEDARIKWKLHGEKGNADLGAGAAHSEALTFIANELLSEELR
SSIGAIGHRIVHGGEQFTSSVVINDDVVKGIEHAIQFAPLHNPAHLIGIKEAFRIFPELKEKNVAVFDTAFHQTMPEEAF
LYALPYKLYKEHGIRRYGAHGTSHLFITSQVAELAGKPVDQTNAIICHLGNGGSVSVVRNGKCIDTSMGLTPLEGLVMGT
RSGDIDPAIVFYLYKNLGMSMEQIEDTLVKKSGLLGLTEVTSDCRYAEDNYEDASKPEAKRALDVYSYRLAKYIGAYMAI
LGDDHLDAIAFTGGIGENSGHVRELALNHLKLFGVKLDVERNLAARFGKSGVITADDSTFKAVVIPTNEELVIAQDTAKL
AL
>Mature_401_residues
SKNLILILNCGSSSLKFAVLDPKTGDEKLSGLAEAFNLEDARIKWKLHGEKGNADLGAGAAHSEALTFIANELLSEELRS
SIGAIGHRIVHGGEQFTSSVVINDDVVKGIEHAIQFAPLHNPAHLIGIKEAFRIFPELKEKNVAVFDTAFHQTMPEEAFL
YALPYKLYKEHGIRRYGAHGTSHLFITSQVAELAGKPVDQTNAIICHLGNGGSVSVVRNGKCIDTSMGLTPLEGLVMGTR
SGDIDPAIVFYLYKNLGMSMEQIEDTLVKKSGLLGLTEVTSDCRYAEDNYEDASKPEAKRALDVYSYRLAKYIGAYMAIL
GDDHLDAIAFTGGIGENSGHVRELALNHLKLFGVKLDVERNLAARFGKSGVITADDSTFKAVVIPTNEELVIAQDTAKLA
L

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=402, Percent_Identity=68.6567164179104, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=41.9191919191919, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_ACTP2 (A3N011)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053352.1
- ProteinModelPortal:   A3N011
- SMR:   A3N011
- STRING:   A3N011
- GeneID:   4848842
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0645
- eggNOG:   COG0282
- HOGENOM:   HBG285863
- OMA:   PHLKDKN
- PhylomeDB:   A3N011
- ProtClustDB:   PRK00180
- BioCyc:   APLE416269:APL_0645-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43583; Mature: 43452

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNLILILNCGSSSLKFAVLDPKTGDEKLSGLAEAFNLEDARIKWKLHGEKGNADLGAG
CCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCC
AAHSEALTFIANELLSEELRSSIGAIGHRIVHGGEQFTSSVVINDDVVKGIEHAIQFAPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCEEEECHHHHHHHHHHHHCCCC
HNPAHLIGIKEAFRIFPELKEKNVAVFDTAFHQTMPEEAFLYALPYKLYKEHGIRRYGAH
CCCHHEEEHHHHHHHHHHHHHCCEEEEEHHHHHCCCCHHEEEECCHHHHHHCCCEEECCC
GTSHLFITSQVAELAGKPVDQTNAIICHLGNGGSVSVVRNGKCIDTSMGLTPLEGLVMGT
CCEEEEEEHHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCEEEECCCCCCCCCCEEEEC
RSGDIDPAIVFYLYKNLGMSMEQIEDTLVKKSGLLGLTEVTSDCRYAEDNYEDASKPEAK
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCHHH
RALDVYSYRLAKYIGAYMAILGDDHLDAIAFTGGIGENSGHVRELALNHLKLFGVKLDVE
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHEEEEEEEECC
RNLAARFGKSGVITADDSTFKAVVIPTNEELVIAQDTAKLAL
CCHHHHCCCCCEEEECCCCEEEEEEECCCCEEEEECCHHHCC
>Mature Secondary Structure 
SKNLILILNCGSSSLKFAVLDPKTGDEKLSGLAEAFNLEDARIKWKLHGEKGNADLGAG
CCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCC
AAHSEALTFIANELLSEELRSSIGAIGHRIVHGGEQFTSSVVINDDVVKGIEHAIQFAPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCEEEECHHHHHHHHHHHHCCCC
HNPAHLIGIKEAFRIFPELKEKNVAVFDTAFHQTMPEEAFLYALPYKLYKEHGIRRYGAH
CCCHHEEEHHHHHHHHHHHHHCCEEEEEHHHHHCCCCHHEEEECCHHHHHHCCCEEECCC
GTSHLFITSQVAELAGKPVDQTNAIICHLGNGGSVSVVRNGKCIDTSMGLTPLEGLVMGT
CCEEEEEEHHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCEEEECCCCCCCCCCEEEEC
RSGDIDPAIVFYLYKNLGMSMEQIEDTLVKKSGLLGLTEVTSDCRYAEDNYEDASKPEAK
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCHHH
RALDVYSYRLAKYIGAYMAILGDDHLDAIAFTGGIGENSGHVRELALNHLKLFGVKLDVE
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHEEEEEEEECC
RNLAARFGKSGVITADDSTFKAVVIPTNEELVIAQDTAKLAL
CCHHHHCCCCCEEEECCCCEEEEEEECCCCEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA