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The map label for this gene is guaA

Identifier: 126208074

GI number: 126208074

Start: 675066

End: 676637

Strand: Reverse

Name: guaA

Synonym: APL_0592

Alternate gene names: 126208074

Gene position: 676637-675066 (Counterclockwise)

Preceding gene: 126208075

Following gene: 126208072

Centisome position: 29.75

GC content: 43.45

Gene sequence:

>1572_bases
ATGACCAACATTCACAATCATAAAATTTTAATTTTGGACTTCGGTTCACAATACACGCAATTAATCGCACGTCGTGTGCG
TGAGATTGGGGTTTATTGCGAGCTTTGGGCGTGGGATGTCACCGAAGAACAAATCCGTGAATTTAACCCGACCGGGATCA
TTCTTTCAGGTGGTCCTGAAAGTACCACTGAAGAAAACAGCCCTCGTGCGCCGGAGTATGTATTTAACGCAGGCGTGCCA
GTACTTGGTATTTGCTATGGTATGCAAACCATGGCGATGCAATTAGGCGGTTTAACTGAAACTTCTGATCATCGTGAATT
CGGTTATGCTTCCGTTGATTTACAAGCGACCGATGCGTTATTTGCAAAATTAAACGATAATTTGACCGCTTCCGAGCCGA
AATTAGACGTTTGGATGAGCCACGGCGATAAAGTAACTCGTTTACCGCAAGGTTTCCAAGTAACAGGTATAACGCCAACT
TGCCCGATTGCAGCAATGTCAGACGAAAGCCGTCGTTTCTACGGTGTGCAATTCCACCCGGAAGTAACTCATACGAAAAG
CGGTTTAGAGTTATTAACTAACTTCGTAGTAGGCATTTGTGGTTGCGAATGTAAATGGACGGCAGAAAATATTATCGAAC
GCCGTGTCGCTCGTATTAAAGAGCAAGTAGGCGATGACGAAGTCATTTTAGGTTTATCAGGCGGCGTGGATTCATCAGTA
ACCGCACTTCTTCTACATCGTGCAATCGGCAAAAACTTACACTGCGTATTCGTGGATAACGGTTTACTTCGTTTAAACGA
AGGCGATCAAGTGATGGAAATGTTCGGCGATAAATTCGGTTTAAATATCATTCGTGTAAATGCCGAAGACCGTTTCTTAG
ATGCGTTAAAAGGTATTGATGAACCGGAAGCAAAACGTAAAACCATCGGTAAAGTGTTTGTCGATGTATTCGATGATGAG
TCGAAAAAACTCACTTCAGTAAAATGGTTAGCACAAGGCACAATCTACCCTGACGTTATCGAATCTGCTGCGAGCAAAAC
CGGTAAAGCACACGTGATTAAATCTCACCATAACGTAGGCGGCTTACCGGATTATATGAAACTCGGTTTAGTGGAACCGT
TACGTGAATTATTCAAAGATGAAGTACGTAAAATTGGTTTAGCACTTGGTTTACCGGCAGAAATGTTAAATCGCCACCCA
TTCCCGGGTCCGGGTTTAGGTGTGCGTGTATTAGGTGAAATCAAGAAAGAATACTGCGATTTACTGCGTAAAGCGGATGC
AATCTTTATCGAAGAGCTCTACAAAGCGGATTGGTATTACAAAGTAAGCCAAGCGTTTACCGTATTCCTTCCGGTAAAAT
CGGTGGGTGTAATGGGTGACGGTCGTAAATACGACTGGGTTGTTTCACTCCGTGCGGTAGAAACTATCGACTTTATGACC
GCACATTGGGCGCATTTACCATACGATTTACTCGGTAAAATCTCAAACCGTATTATCAATGAAGTAAACGGCATTTCTCG
CGTGGTATATGACGTTTCAGGTAAACCGCCTGCAACGATTGAGTGGGAATAA

Upstream 100 bases:

>100_bases
GTAATCATACCGAAAAATTTGATATTTATTATATTGCTGATCCCAAAGGTGGAACTTACCTTTCAATAAAACCAATTAAA
CAATAAAACAGAGAAACAAA

Downstream 100 bases:

>100_bases
AAAATCCGCTTTTCAGTTAATTTCAATTCATTTCAAAAATAGCTCAAAGCCCTGTAAACACAGGGCTTTTCTTTATGCTA
ATTAGCAATCTTGTTTGCCG

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MTNIHNHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQIREFNPTGIILSGGPESTTEENSPRAPEYVFNAGVP
VLGICYGMQTMAMQLGGLTETSDHREFGYASVDLQATDALFAKLNDNLTASEPKLDVWMSHGDKVTRLPQGFQVTGITPT
CPIAAMSDESRRFYGVQFHPEVTHTKSGLELLTNFVVGICGCECKWTAENIIERRVARIKEQVGDDEVILGLSGGVDSSV
TALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFGDKFGLNIIRVNAEDRFLDALKGIDEPEAKRKTIGKVFVDVFDDE
SKKLTSVKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLPAEMLNRHP
FPGPGLGVRVLGEIKKEYCDLLRKADAIFIEELYKADWYYKVSQAFTVFLPVKSVGVMGDGRKYDWVVSLRAVETIDFMT
AHWAHLPYDLLGKISNRIINEVNGISRVVYDVSGKPPATIEWE

Sequences:

>Translated_523_residues
MTNIHNHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQIREFNPTGIILSGGPESTTEENSPRAPEYVFNAGVP
VLGICYGMQTMAMQLGGLTETSDHREFGYASVDLQATDALFAKLNDNLTASEPKLDVWMSHGDKVTRLPQGFQVTGITPT
CPIAAMSDESRRFYGVQFHPEVTHTKSGLELLTNFVVGICGCECKWTAENIIERRVARIKEQVGDDEVILGLSGGVDSSV
TALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFGDKFGLNIIRVNAEDRFLDALKGIDEPEAKRKTIGKVFVDVFDDE
SKKLTSVKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLPAEMLNRHP
FPGPGLGVRVLGEIKKEYCDLLRKADAIFIEELYKADWYYKVSQAFTVFLPVKSVGVMGDGRKYDWVVSLRAVETIDFMT
AHWAHLPYDLLGKISNRIINEVNGISRVVYDVSGKPPATIEWE
>Mature_522_residues
TNIHNHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQIREFNPTGIILSGGPESTTEENSPRAPEYVFNAGVPV
LGICYGMQTMAMQLGGLTETSDHREFGYASVDLQATDALFAKLNDNLTASEPKLDVWMSHGDKVTRLPQGFQVTGITPTC
PIAAMSDESRRFYGVQFHPEVTHTKSGLELLTNFVVGICGCECKWTAENIIERRVARIKEQVGDDEVILGLSGGVDSSVT
ALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFGDKFGLNIIRVNAEDRFLDALKGIDEPEAKRKTIGKVFVDVFDDES
KKLTSVKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLPAEMLNRHPF
PGPGLGVRVLGEIKKEYCDLLRKADAIFIEELYKADWYYKVSQAFTVFLPVKSVGVMGDGRKYDWVVSLRAVETIDFMTA
HWAHLPYDLLGKISNRIINEVNGISRVVYDVSGKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=568, Percent_Identity=34.6830985915493, Blast_Score=287, Evalue=2e-77,
Organism=Escherichia coli, GI1788854, Length=522, Percent_Identity=78.544061302682, Blast_Score=858, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71992710, Length=575, Percent_Identity=35.304347826087, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI71992717, Length=575, Percent_Identity=35.304347826087, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI133901714, Length=575, Percent_Identity=35.304347826087, Blast_Score=288, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6323873, Length=524, Percent_Identity=48.6641221374046, Blast_Score=513, Evalue=1e-146,
Organism=Drosophila melanogaster, GI281365319, Length=562, Percent_Identity=35.5871886120996, Blast_Score=288, Evalue=6e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_ACTP2 (A3MZV8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053299.1
- ProteinModelPortal:   A3MZV8
- SMR:   A3MZV8
- STRING:   A3MZV8
- GeneID:   4849920
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0592
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- PhylomeDB:   A3MZV8
- ProtClustDB:   PRK00074
- BioCyc:   APLE416269:APL_0592-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 58319; Mature: 58187

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 85-85 ACT_SITE 179-179 ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIHNHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQIREFNPTGIILSGGPE
CCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEEECCCHHHHHCCCCCEEEEECCCC
STTEENSPRAPEYVFNAGVPVLGICYGMQTMAMQLGGLTETSDHREFGYASVDLQATDAL
CCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHH
FAKLNDNLTASEPKLDVWMSHGDKVTRLPQGFQVTGITPTCPIAAMSDESRRFYGVQFHP
HHHHCCCCCCCCCCEEEEECCCCCEEECCCCCEEECCCCCCCEEECCCCCCEEEEEEECC
EVTHTKSGLELLTNFVVGICGCECKWTAENIIERRVARIKEQVGDDEVILGLSGGVDSSV
CCCCCHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
TALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFGDKFGLNIIRVNAEDRFLDALKGID
HHHHHHHHHCCCEEEEEEECCEEEECCCHHHHHHCCHHHCCEEEEECCCHHHHHHHCCCC
EPEAKRKTIGKVFVDVFDDESKKLTSVKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVG
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCC
GLPDYMKLGLVEPLRELFKDEVRKIGLALGLPAEMLNRHPFPGPGLGVRVLGEIKKEYCD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LLRKADAIFIEELYKADWYYKVSQAFTVFLPVKSVGVMGDGRKYDWVVSLRAVETIDFMT
HHHHHHHHHHHHHHHCCCEEEECCEEEEEEEHHHCCCCCCCCCEEEEEEEEHHHHHHHHH
AHWAHLPYDLLGKISNRIINEVNGISRVVYDVSGKPPATIEWE
HHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEECC
>Mature Secondary Structure 
TNIHNHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQIREFNPTGIILSGGPE
CCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEEECCCHHHHHCCCCCEEEEECCCC
STTEENSPRAPEYVFNAGVPVLGICYGMQTMAMQLGGLTETSDHREFGYASVDLQATDAL
CCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHH
FAKLNDNLTASEPKLDVWMSHGDKVTRLPQGFQVTGITPTCPIAAMSDESRRFYGVQFHP
HHHHCCCCCCCCCCEEEEECCCCCEEECCCCCEEECCCCCCCEEECCCCCCEEEEEEECC
EVTHTKSGLELLTNFVVGICGCECKWTAENIIERRVARIKEQVGDDEVILGLSGGVDSSV
CCCCCHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
TALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFGDKFGLNIIRVNAEDRFLDALKGID
HHHHHHHHHCCCEEEEEEECCEEEECCCHHHHHHCCHHHCCEEEEECCCHHHHHHHCCCC
EPEAKRKTIGKVFVDVFDDESKKLTSVKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVG
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCC
GLPDYMKLGLVEPLRELFKDEVRKIGLALGLPAEMLNRHPFPGPGLGVRVLGEIKKEYCD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LLRKADAIFIEELYKADWYYKVSQAFTVFLPVKSVGVMGDGRKYDWVVSLRAVETIDFMT
HHHHHHHHHHHHHHHCCCEEEECCEEEEEEEHHHCCCCCCCCCEEEEEEEEHHHHHHHHH
AHWAHLPYDLLGKISNRIINEVNGISRVVYDVSGKPPATIEWE
HHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA