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The map label for this gene is sucB [H]

Identifier: 126207938

GI number: 126207938

Start: 522560

End: 523789

Strand: Reverse

Name: sucB [H]

Synonym: APL_0454

Alternate gene names: 126207938

Gene position: 523789-522560 (Counterclockwise)

Preceding gene: 126207939

Following gene: 126207937

Centisome position: 23.03

GC content: 45.2

Gene sequence:

>1230_bases
ATGACTATTGAAATTTTAACTCCCGTGCTACCCGAATCCGTCGCGGATGCTACCGTTGCGACTTGGCACAAAAAAGTAGG
CGATACGGTTAAACGTGACGAAGTACTTGTTGAAATTGAAACGGATAAAGTCGTTTTAGAAGTGCCGGCACCGAATGACG
GCGTATTAGCGGAAATCAGCCAAGAGCAAGGCGCAACCGTTACTTCTAAACAGTTACTTGGGAAAATCTCAACCGTTCAA
GCGGGAGATTTTACGCAAGAAACTATCAAACAAACTAACGAAGCGACTCCCGCCGATCGTAAATCCGCCGCCATCGAATA
TGATCACTCGGATGCCGACTCTCAAGGTCCGGCAATTCGTCGTTTATTAGCGGAACATAATATCGAAGCGCATTTAGTCA
AAGGTACCGGTGTCGGCGGTCGTATTACCCGTGAAGATATCGAACACTATCTTGCACAACGTCAGGTTCAAGAAACCAAA
CAAGCGATGGCAAGCGAACACAATACGGTAAGTACCGTAGCTTACTCCGCTCGTAGCGAAAAACGCGTGCCGATGACACG
TTTGCGTAAACGTATTGCCGAACGTCTGTTAGAAGCGAAAAACACTACTGCAATGCTGACTACCTTCAACGAAGTGGATA
TGCAGCCGATTATGAGCTTACGTAAAACTTACGGTGAGAAATTTGAAAAACAACACGGCGTGCGTTTAGGCTTTATGTCA
TTCTATATTAAAGCGGTGGTAGAAGCGTTAAAACGTTATCCGGAAATCAATGCTTCGATTGACGGCGATGATGTGGTATA
TCACAACTACTTCGATATTAGTATTGCGGTATCCACTCCGCGCGGATTAGTCACGCCGGTGATTCGTAACTGCGATAAAT
TATCGATGGCGGATATTGAGAAAACCATTAAAGCACTTGCCGAAAAAGGACGTGACGGTAAACTAACCGTAGAAGACCTA
ACCGGCGGTAACTTTACCATTACTAACGGCGGCGTATTCGGTTCGTTAATGTCCACTCCGATTATCAATCCGCCGCAAAG
TGCGATTTTAGGTATGCATGCGATTAAAGATCGCCCTGTCGCAGTAAACGGTCAAGTGGTGATTCGTCCAATGATGTATC
TTGCCTTGTCTTATGACCACCGCTTAATTGATGGACGTGAATCTGTTGGTTTCTTAGTTGCGGTCAAAGATTTATTAGAA
GACCCGACCCGTTTACTACTTGAAATCTAA

Upstream 100 bases:

>100_bases
TTTTAATAAACTTTTGCAAAAAATCTTACGAAAACGACCGCTTACTCAAGTATCGGTTTAGGTAAAACCTATACCGGCAA
ATTAAGAAAGGAACCAAACC

Downstream 100 bases:

>100_bases
TTGCCGTTTTACACTCATTTTTAACCGCTTGTACTTAAACAAGCGGTTATTTTTTGCAAGAAAGTAATATTCACCGAAAT
TTGTAAGATATGTAAACTTA

Product: dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (E2)

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTIEILTPVLPESVADATVATWHKKVGDTVKRDEVLVEIETDKVVLEVPAPNDGVLAEISQEQGATVTSKQLLGKISTVQ
AGDFTQETIKQTNEATPADRKSAAIEYDHSDADSQGPAIRRLLAEHNIEAHLVKGTGVGGRITREDIEHYLAQRQVQETK
QAMASEHNTVSTVAYSARSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNEVDMQPIMSLRKTYGEKFEKQHGVRLGFMS
FYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIAVSTPRGLVTPVIRNCDKLSMADIEKTIKALAEKGRDGKLTVEDL
TGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPVAVNGQVVIRPMMYLALSYDHRLIDGRESVGFLVAVKDLLE
DPTRLLLEI

Sequences:

>Translated_409_residues
MTIEILTPVLPESVADATVATWHKKVGDTVKRDEVLVEIETDKVVLEVPAPNDGVLAEISQEQGATVTSKQLLGKISTVQ
AGDFTQETIKQTNEATPADRKSAAIEYDHSDADSQGPAIRRLLAEHNIEAHLVKGTGVGGRITREDIEHYLAQRQVQETK
QAMASEHNTVSTVAYSARSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNEVDMQPIMSLRKTYGEKFEKQHGVRLGFMS
FYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIAVSTPRGLVTPVIRNCDKLSMADIEKTIKALAEKGRDGKLTVEDL
TGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPVAVNGQVVIRPMMYLALSYDHRLIDGRESVGFLVAVKDLLE
DPTRLLLEI
>Mature_408_residues
TIEILTPVLPESVADATVATWHKKVGDTVKRDEVLVEIETDKVVLEVPAPNDGVLAEISQEQGATVTSKQLLGKISTVQA
GDFTQETIKQTNEATPADRKSAAIEYDHSDADSQGPAIRRLLAEHNIEAHLVKGTGVGGRITREDIEHYLAQRQVQETKQ
AMASEHNTVSTVAYSARSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNEVDMQPIMSLRKTYGEKFEKQHGVRLGFMSF
YIKAVVEALKRYPEINASIDGDDVVYHNYFDISIAVSTPRGLVTPVIRNCDKLSMADIEKTIKALAEKGRDGKLTVEDLT
GGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPVAVNGQVVIRPMMYLALSYDHRLIDGRESVGFLVAVKDLLED
PTRLLLEI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=60.6837606837607, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=29.3598233995585, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=29.3598233995585, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI31711992, Length=439, Percent_Identity=29.1571753986333, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI110671329, Length=422, Percent_Identity=28.1990521327014, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=35.7142857142857, Blast_Score=109, Evalue=6e-24,
Organism=Escherichia coli, GI1786946, Length=410, Percent_Identity=72.9268292682927, Blast_Score=596, Evalue=1e-171,
Organism=Escherichia coli, GI1786305, Length=406, Percent_Identity=31.5270935960591, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=407, Percent_Identity=42.7518427518428, Blast_Score=321, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=31.0502283105023, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=411, Percent_Identity=27.4939172749392, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=325, Percent_Identity=29.5384615384615, Blast_Score=138, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6320352, Length=405, Percent_Identity=45.1851851851852, Blast_Score=339, Evalue=5e-94,
Organism=Saccharomyces cerevisiae, GI6324258, Length=447, Percent_Identity=28.1879194630872, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24645909, Length=225, Percent_Identity=60.8888888888889, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI18859875, Length=420, Percent_Identity=29.7619047619048, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=34.4978165938865, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=34.4978165938865, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45119; Mature: 44988

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEILTPVLPESVADATVATWHKKVGDTVKRDEVLVEIETDKVVLEVPAPNDGVLAEIS
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCCCCEEEEEH
QEQGATVTSKQLLGKISTVQAGDFTQETIKQTNEATPADRKSAAIEYDHSDADSQGPAIR
HCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHH
RLLAEHNIEAHLVKGTGVGGRITREDIEHYLAQRQVQETKQAMASEHNTVSTVAYSARSE
HHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECCCCC
KRVPMTRLRKRIAERLLEAKNTTAMLTTFNEVDMQPIMSLRKTYGEKFEKQHGVRLGFMS
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHH
FYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIAVSTPRGLVTPVIRNCDKLSMADIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHCCCCCHHHHHH
KTIKALAEKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPV
HHHHHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCE
AVNGQVVIRPMMYLALSYDHRLIDGRESVGFLVAVKDLLEDPTRLLLEI
EECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
TIEILTPVLPESVADATVATWHKKVGDTVKRDEVLVEIETDKVVLEVPAPNDGVLAEIS
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCCCCEEEEEH
QEQGATVTSKQLLGKISTVQAGDFTQETIKQTNEATPADRKSAAIEYDHSDADSQGPAIR
HCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHH
RLLAEHNIEAHLVKGTGVGGRITREDIEHYLAQRQVQETKQAMASEHNTVSTVAYSARSE
HHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECCCCC
KRVPMTRLRKRIAERLLEAKNTTAMLTTFNEVDMQPIMSLRKTYGEKFEKQHGVRLGFMS
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHH
FYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIAVSTPRGLVTPVIRNCDKLSMADIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHCCCCCHHHHHH
KTIKALAEKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPV
HHHHHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCE
AVNGQVVIRPMMYLALSYDHRLIDGRESVGFLVAVKDLLEDPTRLLLEI
EECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]