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The map label for this gene is gdhA [H]

Identifier: 126207911

GI number: 126207911

Start: 483824

End: 485164

Strand: Reverse

Name: gdhA [H]

Synonym: APL_0427

Alternate gene names: 126207911

Gene position: 485164-483824 (Counterclockwise)

Preceding gene: 126207912

Following gene: 126207910

Centisome position: 21.33

GC content: 45.34

Gene sequence:

>1341_bases
ATGAGCGACTTAAACGCACTGTTCCAAAAAATCAAACAACGTGACCCTGACCAAGCGCCTTTCCACCAAGCGGTGGAAGA
AGTTTTCGGTTCATTAGCACCATTTCTTGCCAAAAATCCTAAATATACGCAACAAGGTATTTTAGAACGTATCGTTGAGC
CGGAGCGTGTAATTACTTTCCGTGTCACTTGGGTCGATGATAAAGGTCAAGTGCAAGTAAACCGCGGTTATCGTATTCAA
ATGAACTCTGCGATCGGTCCTTATAAAGGCGGTATTCGTTTCCATCCTTCCGTTGATTTAGGGGTTTTAAAATTCTTAGC
GTTTGAACAAGTTTTCAAAAATGCATTAACTACATTACCGATGGGCGGTGGTAAAGGCGGTTCGGATTTCGATCCTAAAG
GTAAATCTGATGCGGAAGTCATGCGTTTCTGCCAAGCATTTATGACGGAATTATCTCGCCATATCGGTGCGGATACCGAC
GTACCGGCGGGCGACATCGGTGTCGGCGGTCGTGAAATCGGTTATATGTACGGTCAATACAAAAAATTACGTAATGAATT
TACCTCGGTCTTAACCGGTAAAAGTTTAACTTGGGGCGGTAGCTTAATTCGTCCGGAAGCAACCGGTTACGGTGCGGTTT
ACTTTGCCGACGCAATGTTAGCAACTCACGGTCAAGGCTTTGACGGCAAACGCGTCGTGATTTCAGGTTCCGGTAACGTA
GCGCAATATGCGGCGGAAAAAGCGATTCAAAAAGGTGCGAAAGTCTTAACCGTATCCGATTCAAACGGTTATGTATTATT
CCCAGATTCGGGTATGACCGAAAAACAATTAGCGGATTTATTAGTACTGAAAAACGAACGCCGTGAACGCTTATCCGTAT
ATGCGAAAGAACAAGGCGTCCAATACTTTGAAGGTCAAAAACCATGGGGCGTTAAATGTGATATTGCGCTGCCGTGTGCG
ACACAAAATGAATTAGACGGCGAAGCGGCGAAAGAATTGGTTAAAAACGGCTGTTTCTGCGTAGCGGAAGGTGCGAATAT
GCCGACCACGCTTGAAGGTGTTGAAGTGTTTATCGCAGCGAAAATTCTTTATGCTCCGGGTAAAGCGGCAAATGCCGGCG
GTGTAGCGACTTCAGGTTTAGAAATGAGCCAAAACGCAATGCGCTTAGCTTGGACTCGTGAAGAAGTGGATCAACGTTTA
TTCAACATTATGACCGCCATTCACCAAAACTGCGTGGAAAACGGTACGGAAACGGACGGTTATGTGAACTATGTGAACGG
TGCGAACATCGCAGGCTTCAAAAAAGTGGCTACCGCAATGTTAGAACAAGGTATCGTATAA

Upstream 100 bases:

>100_bases
TTTCAAATCCAAAATAGCTTGATAGACTCCTTGCCATGCTAAACACACTACTATTTGATAAATATTTTTCATTTTAAATA
TAAATACGCAGGAGTACTTT

Downstream 100 bases:

>100_bases
CAATTTTATATAATATAAACCCTCTTCGGAGGGTTTCTTTTTGCGCAAAATAAAAAGTTTATGCTATGCTGTGACAAACA
GATATAGGAATAAATATGAT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSDLNALFQKIKQRDPDQAPFHQAVEEVFGSLAPFLAKNPKYTQQGILERIVEPERVITFRVTWVDDKGQVQVNRGYRIQ
MNSAIGPYKGGIRFHPSVDLGVLKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELSRHIGADTD
VPAGDIGVGGREIGYMYGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFADAMLATHGQGFDGKRVVISGSGNV
AQYAAEKAIQKGAKVLTVSDSNGYVLFPDSGMTEKQLADLLVLKNERRERLSVYAKEQGVQYFEGQKPWGVKCDIALPCA
TQNELDGEAAKELVKNGCFCVAEGANMPTTLEGVEVFIAAKILYAPGKAANAGGVATSGLEMSQNAMRLAWTREEVDQRL
FNIMTAIHQNCVENGTETDGYVNYVNGANIAGFKKVATAMLEQGIV

Sequences:

>Translated_446_residues
MSDLNALFQKIKQRDPDQAPFHQAVEEVFGSLAPFLAKNPKYTQQGILERIVEPERVITFRVTWVDDKGQVQVNRGYRIQ
MNSAIGPYKGGIRFHPSVDLGVLKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELSRHIGADTD
VPAGDIGVGGREIGYMYGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFADAMLATHGQGFDGKRVVISGSGNV
AQYAAEKAIQKGAKVLTVSDSNGYVLFPDSGMTEKQLADLLVLKNERRERLSVYAKEQGVQYFEGQKPWGVKCDIALPCA
TQNELDGEAAKELVKNGCFCVAEGANMPTTLEGVEVFIAAKILYAPGKAANAGGVATSGLEMSQNAMRLAWTREEVDQRL
FNIMTAIHQNCVENGTETDGYVNYVNGANIAGFKKVATAMLEQGIV
>Mature_445_residues
SDLNALFQKIKQRDPDQAPFHQAVEEVFGSLAPFLAKNPKYTQQGILERIVEPERVITFRVTWVDDKGQVQVNRGYRIQM
NSAIGPYKGGIRFHPSVDLGVLKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELSRHIGADTDV
PAGDIGVGGREIGYMYGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFADAMLATHGQGFDGKRVVISGSGNVA
QYAAEKAIQKGAKVLTVSDSNGYVLFPDSGMTEKQLADLLVLKNERRERLSVYAKEQGVQYFEGQKPWGVKCDIALPCAT
QNELDGEAAKELVKNGCFCVAEGANMPTTLEGVEVFIAAKILYAPGKAANAGGVATSGLEMSQNAMRLAWTREEVDQRLF
NIMTAIHQNCVENGTETDGYVNYVNGANIAGFKKVATAMLEQGIV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=369, Percent_Identity=30.6233062330623, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI4885281, Length=369, Percent_Identity=29.810298102981, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1788059, Length=443, Percent_Identity=65.9142212189616, Blast_Score=606, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI17544676, Length=460, Percent_Identity=27.1739130434783, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324951, Length=451, Percent_Identity=53.6585365853659, Blast_Score=431, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=52.2026431718062, Blast_Score=423, Evalue=1e-119,
Organism=Drosophila melanogaster, GI45549226, Length=363, Percent_Identity=30.0275482093664, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45553475, Length=363, Percent_Identity=30.0275482093664, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24649283, Length=441, Percent_Identity=26.984126984127, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24649460, Length=297, Percent_Identity=29.96632996633, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48494; Mature: 48363

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLNALFQKIKQRDPDQAPFHQAVEEVFGSLAPFLAKNPKYTQQGILERIVEPERVITF
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEE
RVTWVDDKGQVQVNRGYRIQMNSAIGPYKGGIRFHPSVDLGVLKFLAFEQVFKNALTTLP
EEEEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCC
MGGGKGGSDFDPKGKSDAEVMRFCQAFMTELSRHIGADTDVPAGDIGVGGREIGYMYGQY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFADAMLATHGQGFDGKRVVISGSGNV
HHHHHHHHHHHCCCCEECCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCH
AQYAAEKAIQKGAKVLTVSDSNGYVLFPDSGMTEKQLADLLVLKNERRERLSVYAKEQGV
HHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
QYFEGQKPWGVKCDIALPCATQNELDGEAAKELVKNGCFCVAEGANMPTTLEGVEVFIAA
HHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHH
KILYAPGKAANAGGVATSGLEMSQNAMRLAWTREEVDQRLFNIMTAIHQNCVENGTETDG
HHHCCCCCCCCCCCCCCCCCHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YVNYVNGANIAGFKKVATAMLEQGIV
EEEECCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDLNALFQKIKQRDPDQAPFHQAVEEVFGSLAPFLAKNPKYTQQGILERIVEPERVITF
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEE
RVTWVDDKGQVQVNRGYRIQMNSAIGPYKGGIRFHPSVDLGVLKFLAFEQVFKNALTTLP
EEEEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCC
MGGGKGGSDFDPKGKSDAEVMRFCQAFMTELSRHIGADTDVPAGDIGVGGREIGYMYGQY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFADAMLATHGQGFDGKRVVISGSGNV
HHHHHHHHHHHCCCCEECCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCH
AQYAAEKAIQKGAKVLTVSDSNGYVLFPDSGMTEKQLADLLVLKNERRERLSVYAKEQGV
HHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
QYFEGQKPWGVKCDIALPCATQNELDGEAAKELVKNGCFCVAEGANMPTTLEGVEVFIAA
HHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHH
KILYAPGKAANAGGVATSGLEMSQNAMRLAWTREEVDQRLFNIMTAIHQNCVENGTETDG
HHHCCCCCCCCCCCCCCCCCHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YVNYVNGANIAGFKKVATAMLEQGIV
EEEECCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]