Click here to switch to the map view.

The map label for this gene is ecfE [C]

Identifier: 126207896

GI number: 126207896

Start: 470070

End: 471383

Strand: Reverse

Name: ecfE [C]

Synonym: APL_0412

Alternate gene names: 126207896

Gene position: 471383-470070 (Counterclockwise)

Preceding gene: 126207897

Following gene: 126207895

Centisome position: 20.72

GC content: 40.87

Gene sequence:

>1314_bases
ATGACCTCTGTTATTGCATTTTTCATCTTAATTTGCGTACTGGTTTTTGTACATGAATACGGTCACTTTTGGGCTGCTCG
TAAATGCGGCGTCAAAGTGATCCGTTTTTCGATCGGCTTTGGAAAAGTGCTGTTTAAAAAGACTGATAAACACGGCACCG
AATTTGCGTTTTCATTAATTCCGCTCGGCGGCTATGTACAAATGTATAACGGAGAAAACGAACATCAAGCTCGCGCAGAT
CAAACTTTAGCAAGTAAATCCGTTTTACAACGTGCCTTTATTATTGTTGCCGGTCCGTTAGCCAATTTTATATTTGCGAT
ATTGGCTTACTGGTTGGTATTTGCGAACGGCATACCGACTCTAAAACCGGTTACCGGACAGATTTTACCGGATACGATTG
CCTCACAAGCAAAGTTACCGACCGAATTTGAAATTAAACGCGTCGCTTCGCACAACGTACAAGATTGGGAGGAAACGACG
CTTGCGTTAATCGGTTATGTCGGTTCCGATCGTGTAGAAGTTGAAGGCAGTCTGGTCGGTGAAGACCGTTTACAACGTTT
CTATTTGGATTTATCCAATTGGAATGTGGACGGAAATAAAGAAAATCCATTAACTACGCTCGGAATCAGAACAAAAAGCA
GTATCGTTAAACCGGAAATTAAACAAGTTATCGAAAATTCACCGGCGGCAAAAGCCGGATTACAAGCCGGTGATAAAATA
GTCAGCGTGAATCAAACACCTTTCGATTGGGCAGATTTGGTTAAACAGGTTCAAACCGGTCAAATACTCGAATTAACCGT
CGAGAAGTCCGATAATACTTATCGATATTCTCTGCAACCGGATAAAAAAGATGATCGCTATTTTATCGGTATCGTCCCGA
GCTACGAGCCTCTAGCGGATAAGTATCGAACCGAATTAAAATATGATATTCTAACCGCTTTATGGAAAAGCGTTGAAAAA
GTAGGCGCATTGGTTAAAACCATTCTACAATTTATCGGGAACTTAATTACCGGCGAATTATCATTAAAAAATATGGGCGG
TCCTATTTCAATGGCTAAAGGTGCGGGAGCAACCGCCGAAATAGGTTGGGTTTACTATATCAGTTTTATGGCGCTGATTA
GTGTCAATCTTGGCGTAATGAATTTATTCCCAATCTTACCGCTAGACGGAGGACAACTTATTTTACTTGGTGCGGAAGCC
GTTAGAGGAAAACCGCTCGCTGAAAAATTCCAATTACGTTTTCAACAAATCGGTGTATTCTTCGTACTAAGTTTAATGGC
TTTTGCCTTTATGAATGATCTCATTCACTTTTAA

Upstream 100 bases:

>100_bases
TTTAATTCCGGGACACGGCGGTATTTTAGATCGAATTGACAGCTTAACTGCGGCGATTCCGTTCTTTACCACTTTCTTTT
TCTTTGTATTATAGGATGTT

Downstream 100 bases:

>100_bases
TTCCAGGTTATAAGCGGTCGATTCCGCTGATAATTTTGCAAACATTCATAGGATAATTTCAATGCAAAAATTCTTACTTT
CTTCTCTTTTAATTGCAAAC

Product: putative zinc metalloprotease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTSVIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVLFKKTDKHGTEFAFSLIPLGGYVQMYNGENEHQARAD
QTLASKSVLQRAFIIVAGPLANFIFAILAYWLVFANGIPTLKPVTGQILPDTIASQAKLPTEFEIKRVASHNVQDWEETT
LALIGYVGSDRVEVEGSLVGEDRLQRFYLDLSNWNVDGNKENPLTTLGIRTKSSIVKPEIKQVIENSPAAKAGLQAGDKI
VSVNQTPFDWADLVKQVQTGQILELTVEKSDNTYRYSLQPDKKDDRYFIGIVPSYEPLADKYRTELKYDILTALWKSVEK
VGALVKTILQFIGNLITGELSLKNMGGPISMAKGAGATAEIGWVYYISFMALISVNLGVMNLFPILPLDGGQLILLGAEA
VRGKPLAEKFQLRFQQIGVFFVLSLMAFAFMNDLIHF

Sequences:

>Translated_437_residues
MTSVIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVLFKKTDKHGTEFAFSLIPLGGYVQMYNGENEHQARAD
QTLASKSVLQRAFIIVAGPLANFIFAILAYWLVFANGIPTLKPVTGQILPDTIASQAKLPTEFEIKRVASHNVQDWEETT
LALIGYVGSDRVEVEGSLVGEDRLQRFYLDLSNWNVDGNKENPLTTLGIRTKSSIVKPEIKQVIENSPAAKAGLQAGDKI
VSVNQTPFDWADLVKQVQTGQILELTVEKSDNTYRYSLQPDKKDDRYFIGIVPSYEPLADKYRTELKYDILTALWKSVEK
VGALVKTILQFIGNLITGELSLKNMGGPISMAKGAGATAEIGWVYYISFMALISVNLGVMNLFPILPLDGGQLILLGAEA
VRGKPLAEKFQLRFQQIGVFFVLSLMAFAFMNDLIHF
>Mature_436_residues
TSVIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVLFKKTDKHGTEFAFSLIPLGGYVQMYNGENEHQARADQ
TLASKSVLQRAFIIVAGPLANFIFAILAYWLVFANGIPTLKPVTGQILPDTIASQAKLPTEFEIKRVASHNVQDWEETTL
ALIGYVGSDRVEVEGSLVGEDRLQRFYLDLSNWNVDGNKENPLTTLGIRTKSSIVKPEIKQVIENSPAAKAGLQAGDKIV
SVNQTPFDWADLVKQVQTGQILELTVEKSDNTYRYSLQPDKKDDRYFIGIVPSYEPLADKYRTELKYDILTALWKSVEKV
GALVKTILQFIGNLITGELSLKNMGGPISMAKGAGATAEIGWVYYISFMALISVNLGVMNLFPILPLDGGQLILLGAEAV
RGKPLAEKFQLRFQQIGVFFVLSLMAFAFMNDLIHF

Specific function: Unknown

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=438, Percent_Identity=43.1506849315069, Blast_Score=355, Evalue=3e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 48545; Mature: 48414

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS50106 PDZ ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVLFKKTDKHGTEFAFSLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHEECCCCCCEEEEEEE
PLGGYVQMYNGENEHQARADQTLASKSVLQRAFIIVAGPLANFIFAILAYWLVFANGIPT
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHCCCCC
LKPVTGQILPDTIASQAKLPTEFEIKRVASHNVQDWEETTLALIGYVGSDRVEVEGSLVG
CCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHEECCCCEEEECCCCCC
EDRLQRFYLDLSNWNVDGNKENPLTTLGIRTKSSIVKPEIKQVIENSPAAKAGLQAGDKI
HHHHHHHHEECCCCCCCCCCCCCEEEEEECCCHHHCCHHHHHHHCCCCCHHHHCCCCCEE
VSVNQTPFDWADLVKQVQTGQILELTVEKSDNTYRYSLQPDKKDDRYFIGIVPSYEPLAD
EECCCCCCCHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCHHHH
KYRTELKYDILTALWKSVEKVGALVKTILQFIGNLITGELSLKNMGGPISMAKGAGATAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEECCCCCCCHH
IGWVYYISFMALISVNLGVMNLFPILPLDGGQLILLGAEAVRGKPLAEKFQLRFQQIGVF
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHH
FVLSLMAFAFMNDLIHF
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSVIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVLFKKTDKHGTEFAFSLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHEECCCCCCEEEEEEE
PLGGYVQMYNGENEHQARADQTLASKSVLQRAFIIVAGPLANFIFAILAYWLVFANGIPT
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHCCCCC
LKPVTGQILPDTIASQAKLPTEFEIKRVASHNVQDWEETTLALIGYVGSDRVEVEGSLVG
CCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHEECCCCEEEECCCCCC
EDRLQRFYLDLSNWNVDGNKENPLTTLGIRTKSSIVKPEIKQVIENSPAAKAGLQAGDKI
HHHHHHHHEECCCCCCCCCCCCCEEEEEECCCHHHCCHHHHHHHCCCCCHHHHCCCCCEE
VSVNQTPFDWADLVKQVQTGQILELTVEKSDNTYRYSLQPDKKDDRYFIGIVPSYEPLAD
EECCCCCCCHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCHHHH
KYRTELKYDILTALWKSVEKVGALVKTILQFIGNLITGELSLKNMGGPISMAKGAGATAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEECCCCCCCHH
IGWVYYISFMALISVNLGVMNLFPILPLDGGQLILLGAEAVRGKPLAEKFQLRFQQIGVF
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHH
FVLSLMAFAFMNDLIHF
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]