Click here to switch to the map view.

The map label for this gene is lpxA

Identifier: 126207891

GI number: 126207891

Start: 464325

End: 465119

Strand: Reverse

Name: lpxA

Synonym: APL_0407

Alternate gene names: 126207891

Gene position: 465119-464325 (Counterclockwise)

Preceding gene: 126207892

Following gene: 126207888

Centisome position: 20.45

GC content: 44.65

Gene sequence:

>795_bases
ATGCGATTAATTGATTCAACTGCAAAAATCAGTCCTTTAGCCGTCGTTGAAGAAGGGGCGCAAATTGGCGCTCATGTCGA
AATCGGCCCGTTTTCCGTTATCGGCAAAAATGTAAAAATCGGCGCAAAAACAGTTATTCACTCTCATGTTGTGATTAACG
GTCATACCGAAATCGGCGAACAAAATCAAATTTTCCAATTCGCCAGTATCGGGGAAATTAACCAAGATTTAAAATATCAA
GGCGAACCGACTAAAGTGATTATCGGCGATCGTAACCGTATTCGCGAAAACGTTACCATCCACCGCGGAACCGTGCAAGG
CGGCGGTGTTACACGTATCGGCAATGACAACCTGTTTATGATCAACACTCATATTGCGCACGACTGCTCTATCGGTAATC
GTTGTATTATTGCCAATAACGGTACTTTAGCCGGTCATGTTACGTTAGACGATTTCGTTATCGTAGGCGGAATGTCGGCG
ATCCACCAATTCGTCGTTATCGGCTCACACGTAATGTTAGGCGGCGGTTCAATGGTTAGCCAAGACGTACCGCCATACGT
AATGGCACAAGGAAACCATGCTCAACCGTTCGGGGTGAATTTGGAAGGTTTAAAACGTCGCGGCTTTGATAAGCCAGCGA
TGCACGCCATTCGTAATGCGTATAAATTAATTTATCGTAGCGGTAAGACAATTGAAGAAGCAATTCCGGAAATCGAACAA
TTTGCAGTAAACGAACCTGCCGTTCAGCTATTCCTAGATTTCTTTAAACGCTCGACTCGCGGTATTATTCGCTAA

Upstream 100 bases:

>100_bases
TAAATTTACCGGAAAAGCTTTTGTTGATGGAAAACTAGTGTGCGAAGCCGATTTAATGTGTGCACGTAAATAATTAAACT
CCAACCTCACAGGAGAAACT

Downstream 100 bases:

>100_bases
TCGACCGAATAGACAAGCGGTGTAATTTGCAAAGTTTTTAACAAATTACACCGCTTTTTTTATAAATTAATGACCGCTTG
TTCCGCCAATTCTCGTGCCA

Product: UDP-N-acetylglucosamine acyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine acyltransferase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRLIDSTAKISPLAVVEEGAQIGAHVEIGPFSVIGKNVKIGAKTVIHSHVVINGHTEIGEQNQIFQFASIGEINQDLKYQ
GEPTKVIIGDRNRIRENVTIHRGTVQGGGVTRIGNDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPAMHAIRNAYKLIYRSGKTIEEAIPEIEQ
FAVNEPAVQLFLDFFKRSTRGIIR

Sequences:

>Translated_264_residues
MRLIDSTAKISPLAVVEEGAQIGAHVEIGPFSVIGKNVKIGAKTVIHSHVVINGHTEIGEQNQIFQFASIGEINQDLKYQ
GEPTKVIIGDRNRIRENVTIHRGTVQGGGVTRIGNDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPAMHAIRNAYKLIYRSGKTIEEAIPEIEQ
FAVNEPAVQLFLDFFKRSTRGIIR
>Mature_264_residues
MRLIDSTAKISPLAVVEEGAQIGAHVEIGPFSVIGKNVKIGAKTVIHSHVVINGHTEIGEQNQIFQFASIGEINQDLKYQ
GEPTKVIIGDRNRIRENVTIHRGTVQGGGVTRIGNDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPAMHAIRNAYKLIYRSGKTIEEAIPEIEQ
FAVNEPAVQLFLDFFKRSTRGIIR

Specific function: Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell

COG id: COG1043

COG function: function code M; Acyl-[acyl carrier protein]--UDP-N-acetylglucosamine O-acyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxA subfamily

Homologues:

Organism=Escherichia coli, GI1786378, Length=262, Percent_Identity=69.4656488549618, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1786376, Length=209, Percent_Identity=32.5358851674641, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXA_ACTP2 (A3MZC5)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053116.1
- ProteinModelPortal:   A3MZC5
- SMR:   A3MZC5
- STRING:   A3MZC5
- GeneID:   4850034
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0407
- eggNOG:   COG1043
- HOGENOM:   HBG659295
- OMA:   DLKYQGE
- PhylomeDB:   A3MZC5
- ProtClustDB:   PRK05289
- BioCyc:   APLE416269:APL_0407-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00387
- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR010137
- InterPro:   IPR011004
- PIRSF:   PIRSF000456
- TIGRFAMs:   TIGR01852

Pfam domain/function: PF00132 Hexapep; SSF51161 Trimer_LpxA_like

EC number: =2.3.1.129

Molecular weight: Translated: 28736; Mature: 28736

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIDSTAKISPLAVVEEGAQIGAHVEIGPFSVIGKNVKIGAKTVIHSHVVINGHTEIGE
CCCCCCCCCCCCEEEEECCCCCCCEEEECCCEEECCCEEECCEEEEEEEEEEECCCCCCC
QNQIFQFASIGEINQDLKYQGEPTKVIIGDRNRIRENVTIHRGTVQGGGVTRIGNDNLFM
CCCEEEEECCCCCCCCCEECCCCCEEEECCCHHHHCCEEEEECEECCCCEEEECCCCEEE
INTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSAIHQFVVIGSHVMLGGGSMVS
EEECEEECCCCCCEEEEECCCEEEEEEEECCEEEECCHHHHHHHHHHCCEEEECCCCCCC
QDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPAMHAIRNAYKLIYRSGKTIEEAIPEIEQ
CCCCCEEEECCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
FAVNEPAVQLFLDFFKRSTRGIIR
HHCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRLIDSTAKISPLAVVEEGAQIGAHVEIGPFSVIGKNVKIGAKTVIHSHVVINGHTEIGE
CCCCCCCCCCCCEEEEECCCCCCCEEEECCCEEECCCEEECCEEEEEEEEEEECCCCCCC
QNQIFQFASIGEINQDLKYQGEPTKVIIGDRNRIRENVTIHRGTVQGGGVTRIGNDNLFM
CCCEEEEECCCCCCCCCEECCCCCEEEECCCHHHHCCEEEEECEECCCCEEEECCCCEEE
INTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSAIHQFVVIGSHVMLGGGSMVS
EEECEEECCCCCCEEEEECCCEEEEEEEECCEEEECCHHHHHHHHHHCCEEEECCCCCCC
QDVPPYVMAQGNHAQPFGVNLEGLKRRGFDKPAMHAIRNAYKLIYRSGKTIEEAIPEIEQ
CCCCCEEEECCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
FAVNEPAVQLFLDFFKRSTRGIIR
HHCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA