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The map label for this gene is hemA

Identifier: 126207888

GI number: 126207888

Start: 459206

End: 460516

Strand: Reverse

Name: hemA

Synonym: APL_0404

Alternate gene names: 126207888

Gene position: 460516-459206 (Counterclockwise)

Preceding gene: 126207891

Following gene: 126207887

Centisome position: 20.25

GC content: 42.79

Gene sequence:

>1311_bases
ATGACGATTTTAGCACTTGGTATTAACCATAAAACGGCCTCGGTAAATTTGCGAAGTAAGGTGGCGTTTGATGATCAAAA
ACGTCAGCTTGCTTTTGAGCAAATTCAACACCGCGCACTTGCTGAAAGCGTTGTCATTCTTTCAACGTGTAACCGAACAG
AGCTTTATTTTCATAATGCGGAAATTACGCCGCGAGAAGAACATGAAGATAACATCGCATGGCGTGAACAATGTTTTGAA
TGGTTTGCCGAAATTCATCAACTTGAACATAATGAACTACGCGAATGTATTTATTTCAAACAAAATATGGAATCCGCTCG
TCATTTAATGCGAGTAGCGTGCGGTTTAGATTCGCTTATCTTGGGCGAGCCGCAAATTCTAGGTCAGGTAAAACAAGCTT
ATCAATATAGCGAGAATTTTTACCAATCGCAGAACAGCCATATTTCTACTAAATTATCCCGTCTGTTCCAACGTACCTTT
TCGACGGCAAAACGTGTGCGTTCCGAAACGGAAATCGGTTCAAGTGCGGTATCGGTCGCTTATGCCGCTTGCGGTTTGGC
ACGCCAGATTTTCGATAATTTCGGTAAATTGCGTTTCTTGTTGGTCGGTGCCGGCGAAACCATCGAATTAGTCGCGCGTT
ATTTAATTCAGCACGGCGCGAAAAACATTATGATCGCCAACCGTACGCCGCAACGTGCGGAAACGTTAGCGGAGCGTTTA
AACACGCCGATGCAAATTTTATCATTAAGCGCATTGCAAATCGGTTTAAACCAAGCGGATATCGTTATCAGTTCTACCGG
CAGTCCGGATATGCTGATTAGCAAAGAAATGGTGGAAATCGCACAGAAACAACGCCAGTTTGATCCGATGTTATTGATTG
ATATTGCCGTTCCTCGCGATATTGACGAAAATGCCGGCGAATTGGATGCGGTATATTCTTACAGCGTAGATGATTTACAA
CATATCATTCAACGTAATATGGCGCAACGAGAACAAGCTGCAGAACAAGCGGCACAAATTGTGGATGAAGAATGTAAAGC
GTTCTTTGAATGGCTGAAACAACAGCAATCCAGCGATTTGATTAAACGTTATCGCCAAGATGCCGAGCAAACTCGTCAAG
AGCTATTAGCGAAAGCATTAGTTGCCTTAACCAGTGGACAAGATAGTGAAAAAGTCCTTAACGAGCTAAGTTATAAACTG
ACCAACAGCTTATTACACGTGCCGACCCAAGCGTTACAGGCTATGGCGAAGAGCGGAAATTCTCAAGGACTTCAGAGTTT
TTCTAAAGCCTTAAAATTAGAAGAACAATAA

Upstream 100 bases:

>100_bases
AACCAATCTCTACAATTATTTGCGAGAGCCGATTCTATTCGTTAGAATAGGTCGGTTTCAACGGCATTTTGCCGTTTTTT
GTTTTTAGCGTGGGATGTAA

Downstream 100 bases:

>100_bases
CCCAAAAAACTCAATAAACAAGCGGTCGATTTTCGGTAAAATTTTGCAGAAAATCGACCGCTTTTTTTGATCCTTTTTCC
GTTCTCAACGCTTTTAGTAT

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTILALGINHKTASVNLRSKVAFDDQKRQLAFEQIQHRALAESVVILSTCNRTELYFHNAEITPREEHEDNIAWREQCFE
WFAEIHQLEHNELRECIYFKQNMESARHLMRVACGLDSLILGEPQILGQVKQAYQYSENFYQSQNSHISTKLSRLFQRTF
STAKRVRSETEIGSSAVSVAYAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQHGAKNIMIANRTPQRAETLAERL
NTPMQILSLSALQIGLNQADIVISSTGSPDMLISKEMVEIAQKQRQFDPMLLIDIAVPRDIDENAGELDAVYSYSVDDLQ
HIIQRNMAQREQAAEQAAQIVDEECKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLAKALVALTSGQDSEKVLNELSYKL
TNSLLHVPTQALQAMAKSGNSQGLQSFSKALKLEEQ

Sequences:

>Translated_436_residues
MTILALGINHKTASVNLRSKVAFDDQKRQLAFEQIQHRALAESVVILSTCNRTELYFHNAEITPREEHEDNIAWREQCFE
WFAEIHQLEHNELRECIYFKQNMESARHLMRVACGLDSLILGEPQILGQVKQAYQYSENFYQSQNSHISTKLSRLFQRTF
STAKRVRSETEIGSSAVSVAYAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQHGAKNIMIANRTPQRAETLAERL
NTPMQILSLSALQIGLNQADIVISSTGSPDMLISKEMVEIAQKQRQFDPMLLIDIAVPRDIDENAGELDAVYSYSVDDLQ
HIIQRNMAQREQAAEQAAQIVDEECKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLAKALVALTSGQDSEKVLNELSYKL
TNSLLHVPTQALQAMAKSGNSQGLQSFSKALKLEEQ
>Mature_435_residues
TILALGINHKTASVNLRSKVAFDDQKRQLAFEQIQHRALAESVVILSTCNRTELYFHNAEITPREEHEDNIAWREQCFEW
FAEIHQLEHNELRECIYFKQNMESARHLMRVACGLDSLILGEPQILGQVKQAYQYSENFYQSQNSHISTKLSRLFQRTFS
TAKRVRSETEIGSSAVSVAYAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQHGAKNIMIANRTPQRAETLAERLN
TPMQILSLSALQIGLNQADIVISSTGSPDMLISKEMVEIAQKQRQFDPMLLIDIAVPRDIDENAGELDAVYSYSVDDLQH
IIQRNMAQREQAAEQAAQIVDEECKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLAKALVALTSGQDSEKVLNELSYKLT
NSLLHVPTQALQAMAKSGNSQGLQSFSKALKLEEQ

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=435, Percent_Identity=49.4252873563218, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_ACTP2 (A3MZC2)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053113.1
- ProteinModelPortal:   A3MZC2
- SMR:   A3MZC2
- STRING:   A3MZC2
- GeneID:   4849853
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0404
- eggNOG:   COG0373
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- PhylomeDB:   A3MZC2
- ProtClustDB:   PRK00045
- BioCyc:   APLE416269:APL_0404-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 49468; Mature: 49337

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 118-118 BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILALGINHKTASVNLRSKVAFDDQKRQLAFEQIQHRALAESVVILSTCNRTELYFHNA
CEEEEEECCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECC
EITPREEHEDNIAWREQCFEWFAEIHQLEHNELRECIYFKQNMESARHLMRVACGLDSLI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LGEPQILGQVKQAYQYSENFYQSQNSHISTKLSRLFQRTFSTAKRVRSETEIGSSAVSVA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
YAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQHGAKNIMIANRTPQRAETLAERL
HHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHH
NTPMQILSLSALQIGLNQADIVISSTGSPDMLISKEMVEIAQKQRQFDPMLLIDIAVPRD
CCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
IDENAGELDAVYSYSVDDLQHIIQRNMAQREQAAEQAAQIVDEECKAFFEWLKQQQSSDL
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKRYRQDAEQTRQELLAKALVALTSGQDSEKVLNELSYKLTNSLLHVPTQALQAMAKSGN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
SQGLQSFSKALKLEEQ
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TILALGINHKTASVNLRSKVAFDDQKRQLAFEQIQHRALAESVVILSTCNRTELYFHNA
EEEEEECCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECC
EITPREEHEDNIAWREQCFEWFAEIHQLEHNELRECIYFKQNMESARHLMRVACGLDSLI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LGEPQILGQVKQAYQYSENFYQSQNSHISTKLSRLFQRTFSTAKRVRSETEIGSSAVSVA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
YAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQHGAKNIMIANRTPQRAETLAERL
HHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHH
NTPMQILSLSALQIGLNQADIVISSTGSPDMLISKEMVEIAQKQRQFDPMLLIDIAVPRD
CCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
IDENAGELDAVYSYSVDDLQHIIQRNMAQREQAAEQAAQIVDEECKAFFEWLKQQQSSDL
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKRYRQDAEQTRQELLAKALVALTSGQDSEKVLNELSYKLTNSLLHVPTQALQAMAKSGN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
SQGLQSFSKALKLEEQ
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA